Maternal epigenetic pathways control parental contributions to Arabidopsis early embryogenesis.

Defining the contributions and interactions of paternal and maternal genomes during embryo development is critical to understand the fundamental processes involved in hybrid vigor, hybrid sterility, and reproductive isolation. To determine the parental contributions and their regulation during Arabidopsis embryogenesis, we combined deep-sequencing-based RNA profiling and genetic analyses. At the 2-4 cell stage there is a strong, genome-wide dominance of maternal transcripts, although transcripts are contributed by both parental genomes. At the globular stage the relative paternal contribution is higher, largely due to a gradual activation of the paternal genome. We identified two antagonistic maternal pathways that control these parental contributions. Paternal alleles are initially downregulated by the chromatin siRNA pathway, linked to DNA and histone methylation, whereas transcriptional activation requires maternal activity of the histone chaperone complex CAF1. Our results define maternal epigenetic pathways controlling the parental contributions in plant embryos, which are distinct from those regulating genomic imprinting.

Epigenetic control of plant development: new layers of complexity.

Important aspects of plant development are under epigenetic control, that is, under the control of heritable changes in gene expression that are not associated with alterations in DNA sequence. It is becoming clear that RNA molecules play a key role in epigenetic gene regulation by providing sequence specificity for the targeting of developmentally important genes. RNA-based control of gene expression can be exerted posttranscriptionally by interfering with transcript stability or translation. Moreover, RNA molecules also appear to direct developmentally relevant gene regulation at the transcriptional level by modifying chromatin structure and/or DNA methylation.