Visual and morphometric description of feral horse hooves from Sable Island National Park Reserve.

Objective: To visually evaluate and morphometrically describe a sample of abnormal hooves from Sable Island horses that died between 2007 and 2013. Animal: Equine. Procedure: An opportunistic sample of 356 abnormal hooves from horses inhabiting Sable Island National Park Reserve (Canada) was collected postmortem between 2007 and 2013 from 101 Sable Island horses. These hooves were assessed for abnormalities in conformation through visual and morphometric evaluation. Results: On visual and morphometric evaluation, 100% of horses (101/101) had abnormal hoof conformation when compared to a domestic horse hoof model. Specific abnormalities from visual evaluation included long heels (85%); medial, lateral, and dorsal flares (45, 78, and 46% of horses, respectively); and cracking (62%). Specific abnormalities determined from morphometry of the hooves included contracted heels (87%), hoof imbalances including mediolateral imbalance (61%), and hoof length greater than width (100%). Conclusion: Sable Island horses had various hoof abnormalities such as hoof capsule flaring; long, contracted heels; and mediolateral imbalance. These abnormalities have been observed in other feral horse populations living on soft substrates. Given the potential impact of hoof abnormalities on the welfare status of the Sable Island horses, further studies are warranted, including estimation of the prevalence of hoof abnormalities and direct observation of horse gait and behavior. Clinical relevance: The significance of the hoof abnormalities for the health of the Sable Island horses is difficult to determine but the issue highlights the importance of further study to investigate the impact on the horses' welfare.

Description visuelle et morphométrique des sabots de chevaux sauvages de la réserve de parc national de l'Île-de-Sable. Objectif: Évaluer visuellement et décrire morphométriquement un échantillon de sabots anormaux de chevaux de l'île de Sable décédés entre 2007 et 2013. Animal: Cheval. Procédure: Un échantillon opportuniste de 356 sabots anormaux provenant de chevaux habitant la réserve de parc national de l'Île-de-Sable (Canada) a été prélevé post-mortem entre 2007 et 2013 sur 101 chevaux de l'île-de-Sable. Ces sabots ont été évalués pour déceler des anomalies de conformation par une évaluation visuelle et morphométrique. Résultats: Lors de l'évaluation visuelle et morphométrique, 100 % des chevaux (101/101) présentaient une conformation de sabot anormale par rapport à un modèle de sabot de cheval domestique. Les anomalies spécifiques de l'évaluation visuelle comprenaient les talons longs (85 %); poussées médiales, latérales et dorsales (respectivement 45, 78 et 46 % des chevaux); et fissuration (62 %). Les anomalies spécifiques déterminées à partir de la morphométrie des sabots comprenaient des talons contractés (87 %), des déséquilibres des sabots, notamment un déséquilibre médiolatéral (61 %) et une longueur du sabot supérieure à la largeur (100 %). Conclusion: Les chevaux de l'île-de-Sable présentaient diverses anomalies aux sabots, telles qu'un évasement de la boîte cornée; talons longs et contractés; et déséquilibre médiolatéral. Ces anomalies ont été observées chez d'autres populations de chevaux sauvages vivant sur des substrats mous. Compte tenu de l'impact potentiel des anomalies des sabots sur le bien-être des chevaux de l'Île-de-Sable, d'autres études sont justifiées, notamment l'estimation de la prévalence des anomalies des sabots et l'observation directe de la démarche et du comportement des chevaux. Pertinence clinique: L'importance des anomalies des sabots pour la santé des chevaux de l'Île-de-Sable est difficile à déterminer, mais la question souligne l'importance d'une étude plus approfondie pour étudier l'impact sur le bien-être des chevaux.(Traduit par Dr Serge Messier).

Veterinarians' Self-Reported Needs and Attitudes on the Atlantic Canada Veterinary College and Associated Teaching Hospital in Relation to Continuing Education, Research, and Clinical Referrals.

The relationship between veterinary colleges (VCs), associated veterinary teaching hospital referral centers (VTHs), and referring veterinarians (rVets) is important and complex. Despite this, there has been minimal research on rVets with respect to their regional VC and VTH. Our objectives were to evaluate rVets' attitudes and self-reported needs on their regional VC's VTH surrounding (a) continuing education, (b) collaborative research, and (c) referrals for specialty consultation and care. Participants (rVets) were invited to complete an online questionnaire (2018-2019). Respondents (n = 56) indicated multiple (24) unmet continuing education topic needs, of which dermatology and behavior were most selected. Many rVets (70%) reported an interest in collaborative research participation. Communication was frequently selected by respondents when asked what could be improved with respect to the VTH referral center process. Our work provides a benchmark attitudes and needs assessment, identifies areas for improvement, and may aid initiatives regionally, and likely externally, as adapted to other rVet-VC-VTH relationships.

Impact of a Spectrum of Care Elective Course on Third-Year Veterinary Students' Self-Reported Knowledge, Attitudes, and Competencies.

Veterinary services' rising cost is an increasing barrier to pet care. Spectrum of care (SpOC) refers to evidence-based veterinary medicine options along the socioeconomic spectrum. To meet growing pet owner financial constraints and pet care needs, training to equip veterinarians with competencies to provide SpOC as Day One graduates is argued to be added as part of the veterinary curriculum. Objectives of our prospective pre- and post-survey study were to (a) determine baseline self-reported knowledge, attitudes, and competencies (KACs) surrounding SpOC in third-year DVM students; (b) develop and assess impact of a SpOC course on student self-reported SpOC KACs; and (c) obtain student feedback on the course and future SpOC training. Enrolled students (n = 35) completed the pre-survey (n = 35) and post-survey (n = 33). Results indicated that students were aware of the need for SpOC training within the veterinary curriculum, and positive changes occurred in self-reported KAC from pre- to post-survey. Students tended (p = .08) to predict better outcomes in SpOC cost-barrier scenarios from pre- (34%) to post-survey (76%), such as reduced perceived likelihood of euthanasia (63%-39%) and unsuccessful outcomes (40%-27%). Most students (31/33, 94%) predicted the course would benefit them in clinical practice and had preferred future training preferences (online modules [70%], seminars [60%], webinars [58%]). Data indicate benefits in student self-reported KACs following the SpOC course, warranting formal course inclusion, with tracking of students into clinical practice to document objective KAC impacts and perhaps similar course rollout to other institutions.

Companion animal preventive care at a veterinary teaching hospital - Knowledge, attitudes, and practices of clients.

Preventive care is the cornerstone of health. However, veterinary staff to client (pet owner) communication of disease prevention may be limited resulting in increased pet risk. Our objectives were to evaluate knowledge, attitudes, and practices of clients regarding vaccination and parasite control and describe information sources influencing client preventive care. Over a 6-week period, clients visiting a veterinary teaching hospital in Prince Edward Island, Canada, were invited to complete a written questionnaire. Of those invited, 81% (105/129) completed the questionnaire. Respondents reported low (19 to 33%) to moderate (66 to 79%) coverage for canine "lifestyle" and core vaccines, respectively. Half of the participants reported that they had concern for their pet's health from endo/ectoparasites compared to concern for their/household member's health (27%), despite 45% reporting a person at increased zoonotic risk in their household. Veterinarians (89 to 92%) and online information (39 to 51%) were the highest client-reported resources for vaccine and parasite education. Our work provides a baseline for preventive care practices and highlights a need for improvement.

Soins préventifs des animaux de compagnie dans un hôpital d'enseignement vétérinaire ­ Connaissances, attitudes et pratiques des clients. Les soins préventifs sont la pierre angulaire de la santé. Cependant, la communication entre le personnel vétérinaire et le client (propriétaire de l'animal) concernant la prévention des maladies peut être limitée, ce qui augmente le risque pour les animaux de compagnie. Les objectifs de l'étude étaient d'évaluer les connaissances, les attitudes et les pratiques des clients en matière de vaccination et de lutte antiparasitaire et de décrire les sources d'information influençant les soins préventifs prodigués par les clients. Sur une période de 6 semaines, les clients visitant un hôpital d'enseignement vétérinaire de l'Île-du-Prince-Édouard, au Canada, ont été invités à remplir un questionnaire écrit. Parmi les invités, 81 % (105/129) ont rempli le questionnaire. Les répondants ont rapporté une couverture faible (19 à 33 %) à modérée (66 à 79 %) pour les vaccins « style de vie ¼ canin et de base, respectivement. La moitié des participants ont déclaré s'inquiéter pour la santé de leur animal de compagnie relativement aux endo/ectoparasites comparativement à des inquiétudes pour leur santé ou celle des membres de leur famille (27 %), bien que 45 % aient déclaré qu'une personne présentait un risque zoonotique accru dans leur ménage. Les vétérinaires (89 à 92 %) et les renseignements en ligne (39 à 51 %) étaient les ressources les plus importantes déclarées par les clients pour l'éducation sur les vaccins et les parasites. Notre travail fournit une base pour les pratiques de soins préventifs et met en évidence un besoin d'amélioration.(Traduit par Dr Serge Messier).

Veterinary-Prescribed Buprenorphine Ingestion in a 2-Year-Old Girl.

INTRODUCTION: Ingestion of buprenorphine by young children is on the rise and can lead to life-threatening consequences and death. Exposure most often occurs when a child acquires the medication intended for adult use. However, buprenorphine is also prescribed by veterinarians and may be sent home, typically in non-child-resistant packaging, to be administered to the family pet. CASE: A previously healthy 2-year-old girl weighing 11.36 kg was found with a 1-mL syringe containing 0.6 mg/mL of buprenorphine in her mouth. The syringe had been in a plastic bag provided to the family by their veterinarian for the family dog. She was hospitalized for 24 hours but remained asymptomatic and was discharged healthy. This type of exposure to buprenorphine has not previously been described in the literature. CONCLUSIONS: Having this unsecured medication in the home increases the potential risk of exposure for young children and associated health consequences. Pediatricians should be aware of the potential dangers that veterinary pharmaceuticals can pose and educate parents about proper storage of medications. In addition, veterinarians should take extra precautions when dispensing these medications to pet owners with children.

Clients' knowledge, attitudes, and practices related to pet nutrition and exercise at a teaching hospital.

Nutrition and exercise are key components of an animal's overall health; however, these topics may not be discussed with clients during veterinary visits resulting in suboptimal practices. This study sought to i) estimate small animal clientele knowledge, attitudes, and practices on nutrition and exercise; and ii) evaluate client recall of (and interest in) veterinary recommendations related to nutrition and exercise. Clients visiting the Atlantic Veterinary College Small Animal Teaching Hospital over a 1-month period were invited to complete a 5-minute written questionnaire. Of the clients invited to participate, 71% (101/143) completed the questionnaire. An unexpectedly high proportion of clients reported feeding homemade (37%) and raw meat-based diets and products (30%). Most participants indicated an interest in veterinary directed discussions on nutrition (63%) and exercise (63%). Our study provides a benchmark for needed ongoing (and targeted) client education and has led to expansion of our hospital infection control policy.

Connaissances des clients, attitudes et pratiques reliées à la nutrition animale et l'exercice à un hôpital vétérinaire d'enseignement. La nutrition et l'exercice sont des composantes clés de la santé globale d'un animal; toutefois, ces sujets peuvent ne pas être discutés avec les clients lors des visites vétérinaires ce qui résulte en des pratiques suboptimales. La présente étude visait à i) estimer les connaissances de la clientèle des animaux de compagnie, les attitudes et les pratiques sur la nutrition et l'exercice; et ii) évaluer les souvenirs des clients (et leur intérêt) sur les recommandations vétérinaires relatives à la nutrition et l'exercice. Les clients visitant l'hôpital d'enseignement pour animaux de compagnie de l'Atlantic Veterinary College sur une période de 1 mois furent invités à compléter un questionnaire écrit de 5 minutes. Parmi les clients invités à participer, 71 % (101/143) complétèrent le questionnaire. Une proportion élevée inattendue des clients rapporta nourrir les animaux avec une diète maison (37 %) et une diète et des produits à base de viande crue (30 %). La majorité des participants indiqua un intérêt pour des discussions vétérinaires dirigées sur la nutrition (63 %) et l'exercice (63 %). Notre étude fournit une mesure étalon sur la nécessité de poursuivre (et cibler) l'éducation des clients et a mené à une augmentation des mesures de prévention des infections dans notre hôpital.(Traduit par Dr Serge Messier).

Development of an OIE Harmonized Day 1 Competency-Based Veterinary School Curriculum in Ethiopia: A Partnership Model for Curriculum Evaluation and Implementation.

The University of Gondar College of Veterinary Medicine and Animal Sciences (UoG-CVMASc) and the Ohio State University College of Veterinary Medicine (OSU-CVM) developed an objective methodology to assess the curriculum of veterinary institutions and implement changes to create a curriculum that is harmonized with OIE standards while also covering the needs and realities of Gondar and Ethiopia. The process, developed under the sponsorship of the World Organisation for Animal Health (OIE) Veterinary Education Twinning Programme, is outlined in this article with the hope that it can be applied by other countries wishing to improve national veterinary services (VS) through the improvement of their academic programs. The plan created by the UoG-OSU Twinning team consisted of an in-depth curriculum assessment and development process, which entailed three consecutive stages. Stage 1 (Curriculum Assessment) included the design and development of an Evaluation Tool for OIE Day 1 Graduating Veterinarian Competencies in recent graduates, and the mapping and evaluation of the current UoG-CVMASc curriculum based on the OIE Veterinary Education Core Curriculum. Stage 2 (Curriculum Development) consisted of the identification and prioritization of possible solutions to address identified curriculum gaps as well as the development of an action plan to revise and update the curriculum. Finally, Stage 3 (Curriculum Implementation) focused on the process to launch the new curriculum. In September 2017, 53 first-year students started the professional program at the UoG-CVMASc as the first cohort to be accepted into the newly developed OIE Harmonized Curriculum, the first of its kind in Africa.

A cross-sectional study of environmental, dog, and human-related risk factors for positive canine leptospirosis PCR test results in the United States, 2009 to 2016.

BACKGROUND: Canine leptospirosis is a reemerging zoonotic disease concern in North America, and a better understanding of its epidemiology is needed. Wide-scale use and subsequent analyses of polymerase chain reaction (PCR) testing may provide additional insight into leptospirosis. This study aimed to describe temporal trends, to descriptively map, and to identify environmental, dog, and human-level factors associated with positive canine leptospirosis PCR test results in the United States. RESULTS: Data obtained from IDEXX Laboratories, Inc. on 40,118 canine leptospirosis PCR tests run in the United States between 2009 and 2016 were evaluated. Climate and socioeconomic (e.g. urban influence, income) data were obtained from public databases. Choropleth maps were created to identify high test-positive proportion areas and a cross-sectional analysis was completed with generalized (univariable, followed by multivariable) mixed logistic regression models accounting for county within state to identify significant predictors for a positive test. Overall test-positive proportion was 5.4% across the United States, with the regional point estimate highest in the southwest (8.1%). In the final multivariable model, the odds of a positive test were greater for male dogs (Odds Ratio [OR] = 1.28) and dogs 0-4 years of age (ORs ranged from 0.35-0.71 for the other age groups). The odds of a positive test were greater for dogs living in areas with wet environmental conditions (OR = 1.24). Season and temperature, as well as the interaction between them, were significant predictors of a positive test. Dogs had a greater probability of testing positive during cool temperatures (< 4 °C) compared to the other temperature categories in the fall season. CONCLUSIONS: These findings based on PCR testing allow for an improved understanding of factors influencing a positive canine leptospirosis PCR test and will assist targeted education and prevention efforts.

Maintenance of Carbapenemase-Producing Enterobacteriaceae in a Farrow-to-Finish Swine Production System.

Carbapenemase-producing Enterobacteriaceae (CPE) threaten both agriculture and public health. While carbapenems are restricted in food-producing animals, other ß-lactams, such as ceftiofur, are frequently applied in livestock. While the relationship is not fully elucidated, ceftiofur use may provide selective pressure that promotes carbapenem resistance. Recently reported in U.S. livestock, plasmid-mediated CPE are also present in livestock in Europe and Asia. We previously reported the rare carbapenemase gene, blaIMP-64, in the environment of a large farrow-to-finish swine operation. To better understand CPE in this swine production system, in 2016 we followed a cohort of 350+ pigs over 5 months from late sow gestation to the final finishing phase. We screened both environmental and fecal samples for CPE using our selective enrichment protocol, with resulting phenotypic CPE isolates further characterized. Of 55 environmental and 109 sow fecal samples collected from a farrowing barn on our initial visit, 35 (64%) environmental and 15 (14%) sow fecal samples yielded isolates of multiple Enterobacteriaceae species carrying the metallo-ß-lactamase gene blaIMP-64 on an IncQ plasmid. The frequency of IMP-64-positive environmental (n = 32), sow fecal (n = 30), and piglet fecal swab (n = 120) samples was highest for all groups when the market pig cohort was between 1 and 10 days, with observed prevalence of 97%, 28%, and 18%, respectively. After weaning, blaIMP-64 was detected in a single environmental sample from a nursery pen, with no CPE recovered in the finishing phase. Used in U.S. swine production to treat and control disease, ceftiofur is administered to piglets on this farm at birth, with males receiving a second dose at castration (≈day 7). Once introduced into animal agriculture, the common use of ceftiofur may provide the selection pressure required for CPE dissemination throughout large, intensively managed food animal populations housed in animal-dense environments.

Health Benefits and Risks of Pets in Nursing Homes: A Survey of Facilities in Ohio.

Pets are encountered in nursing homes and although they provide health benefits to individuals, they are also a source of health risks. The current study aimed to determine the frequency and types of animals in nursing homes, perceived benefits, and content of policies addressing health risks. Ninety-five administrators from unique nursing homes in Ohio completed an online survey addressing perceived benefits and risks of animals and policies in place. Animals were permitted in 99% of nursing homes, with dogs (95%), cats (85%), birds (71%), fish (55%), and farm animals (40%) most frequently reported. Respondents perceived animal interactions resulted in high health benefits for residents. Most facilities (70/75; 93%) reported having an animal policy, yet important gaps were frequently identified in the content of policies. Most respondents (75%) did not report health and safety concerns with animals in facilities. Best practice guidelines and policies should be developed and implemented in nursing homes to address requirements for different animal ownership models, range of animal species, and staff knowledge. [Journal of Gerontological Nursing, 44(5), 39-45.].

Carbapenemase-Producing Enterobacteriaceae Recovered from the Environment of a Swine Farrow-to-Finish Operation in the United States.

Carbapenem-resistant Enterobacteriaceae (CRE) present an urgent threat to public health. While use of carbapenem antimicrobials is restricted for food-producing animals, other ß-lactams, such as ceftiofur, are used in livestock. This use may provide selection pressure favoring the amplification of carbapenem resistance, but this relationship has not been established. Previously unreported among U.S. livestock, plasmid-mediated CRE have been reported from livestock in Europe and Asia. In this study, environmental and fecal samples were collected from a 1,500-sow, U.S. farrow-to-finish operation during 4 visits over a 5-month period in 2015. Samples were screened using selective media for the presence of CRE, and the resulting carbapenemase-producing isolates were further characterized. Of 30 environmental samples collected from a nursery room on our initial visit, 2 (7%) samples yielded 3 isolates, 2 sequence type 218 (ST 218) Escherichia coli and 1 Proteus mirabilis, carrying the metallo-ß-lactamase gene blaIMP-27 on IncQ1 plasmids. We recovered on our third visit 15 IMP-27-bearing isolates of multiple Enterobacteriaceae species from 11 of 24 (46%) environmental samples from 2 farrowing rooms. These isolates each also carried blaIMP-27 on IncQ1 plasmids. No CRE isolates were recovered from fecal swabs or samples in this study. As is common in U.S. swine production, piglets on this farm receive ceftiofur at birth, with males receiving a second dose at castration (≈day 6). This selection pressure may favor the dissemination of blaIMP-27-bearing Enterobacteriaceae in this farrowing barn. The absence of this selection pressure in the nursery and finisher barns likely resulted in the loss of the ecological niche needed for maintenance of this carbapenem resistance gene.

Canine oral papillomavirus outbreak at a dog daycare facility.

This report describes an outbreak at a dog daycare facility where 13 of 52 dogs developed suspected canine papillomavirus (CPV) infection. Based on contact tracing, subclinical CPV shedding was speculated. Active surveillance, exclusion of animals with active or recent infection and cohort formation may have been effective in stopping the outbreak.

Éclosion du papillomavirus oral canin dans une garderie canine. Ce rapport décrit l'éclosion dans une garderie canine où 13 des 52 chiens ont développé une infection suspectée par le virus du papillome canin (VPC). En se basant sur le retraçage des contacts, on a émis la supposition d'une excrétion subclinique du VPC. Une surveillance active, l'exclusion des animaux avec une infection active ou récente et la formation d'une cohorte ont pu être efficaces pour freiner l'éclosion.(Traduit par Isabelle Vallières).

Laparoscopic placement and urodynamic effects of an artificial urethral sphincter in cadaveric dogs.

OBJECTIVE: To describe a laparoscopic approach for placement of a percutaneously controlled artificial urethral sphincter (AUS) in female cadaver dogs and compare the change in urethral pressure and lumen diameter after filling the device. STUDY DESIGN: Experimental study. ANIMALS: Canine female cadavers (n = 10). METHODS: A laparoscopic technique was used to implant the AUS in 10 cadaver dogs. Maximum urethral closure pressure (MUCP), cystourethral leak point pressure (CLPP), and urethral luminal area were measured at 0, 25, 50, and 75% cuff inflation. Necropsy was performed after urethral pressure profilometry and cystoscopy data collection to assess for trauma caused by the procedure. RESULTS: Laparoscopic implantation was performed successfully in all 10 cadavers with no evidence of inadvertent trauma. Median MUCP at 0% AUS fill (48.9 cmH2 O) was significantly lower than 75% fill (243.5 cmH2 O). Median CLPP at 0% fill (5.0 cmH2 O) was significantly lower than 75% fill (23.2 cmH2 O). Significant differences were also found comparing urodynamic values 25 to 50%, 25 to 75%, and 50 to 75%. Cystoscopic evaluation revealed progressive decreases in urethral lumen area and significant differences between the urethral luminal area values as the AUS cuff was inflated. CONCLUSION: Laparoscopic placement of an AUS can be performed successfully in cadaver dogs, improved urethral pressure profile parameters, and visibly occluded the urethral lumen. Further studies of laparoscopic placement in clinical cases affected by urethral sphincter mechanism incompetence are warranted.

Knowledge, attitudes, and practices related to pet contact by immunocompromised children with cancer and immunocompetent children with diabetes.

OBJECTIVE: To compare knowledge, attitudes, and risks related to pet contact in households with and without immunocompromised children. STUDY DESIGN: A questionnaire was distributed to parents of children diagnosed with cancer (immunocompromised; n=80) or diabetes (immunocompetent; n=251) receiving care at the Children's Hospital of Eastern Ontario. Information was collected on knowledge of pets as sources of disease, concerns regarding pet-derived pathogens, and pet ownership practices. Data were analyzed with multivariable logistic regression. RESULTS: The questionnaire was completed by 65% (214 of 331) of the individuals to whom it was given. Pet ownership was common; 45% of respondents had a household pet when their child was diagnosed, and many (households with a child with diabetes, 49%; households with a child with cancer, 20%) acquired a new pet after diagnosis. Most households that obtained a new pet had acquired a pet considered high risk for infectious disease based on species/age (diabetes, 73%; cancer, 77%). Parents of children with cancer were more likely than parents of children with diabetes to recall being asked by a physician/staff member if they owned a pet (OR, 5.9) or to recall receiving zoonotic disease information (OR, 5.3), yet these interactions were reported uncommonly (diabetes, ≤13%; cancer, ≤48%). Greater knowledge of pet-associated pathogens was associated with recalled receipt of previous education on this topic (OR, 3.9). Pet exposure outside the home was reported frequently for children in non-pet-owning households (diabetes, 48%; cancer, 25%). CONCLUSION: Improved zoonotic disease education is needed for pet-owning and non-pet-owning households with immunocompromised children, with ongoing provision of information while the children are at increased risk of disease. Additional efforts from pediatric and veterinary healthcare professionals are required.

Pet husbandry and infection control practices related to zoonotic disease risks in Ontario, Canada.

BACKGROUND: Many human infections are transmitted through contact with animals (zoonoses), including household pets. Despite this concern, there is limited knowledge of the public's pet husbandry and infection control practices. The objective of this study was to characterize zoonotic disease related-husbandry and infection preventive practices in pet-owning households in Ontario, Canada. METHODS: A self-administered questionnaire was distributed to individuals at two multi-physician clinics in Waterloo, Ontario, Canada during 2010. One adult from each household was invited to participate in the study. RESULTS: Four hundred one pet-owners completed the questionnaire. Households reported ownership of dogs (68%), cats (48%), fish (13%), exotic mammals (7%), such as hamsters, and reptiles and birds (each 6%). Across all species, individuals at higher risk of infections (i.e. < 5 yrs, ≥ 65 yrs, immunocompromised) were often (46-57%) present in households. Children < 16 yrs of age had close pet contact, as households reported dogs (13%) and cats (30%) usually slept in a child's bed and dogs often licked a child's face (24%). Household husbandry practices that increase zoonotic disease risk were frequently identified; some fed high-risk foods (i.e. raw eggs, raw meat, or raw animal product treats) to their dogs (28%) or cats (3%); 14% of reptile-owning households allowed the pet to roam through the kitchen or washed it in the kitchen sink. Reported hand washing by children was high for all species (> 76% washed hands sometimes or greater after touching the pet, its feces, or housing), although fewer reported children always washed their hands (3-57%; by species). With a few exceptions, practices were not associated with the presence of higher risk members in the household or recall of having previously received zoonotic disease education. CONCLUSIONS: The results suggest there is a need for education on zoonotic disease prevention practices for pet-owning households with individuals at higher risk of infection and those with high-risk species (e.g., reptiles). Further research is needed to determine the role of education in altering higher risk pet practices.

Respiratory disease outbreak in a veterinary hospital associated with canine parainfluenza virus infection.

A cluster of canine parainfluenza virus infections was identified in a veterinary referral hospital. While hospital-associated outbreaks of canine parainfluenza virus infection have not been previously reported, veterinary hospitals possess some of the same risk factors that may be present in traditional high-risk sites such as kennels. Hospital-associated transmission of canine respiratory pathogens, therefore, must be considered.

Éclosion de maladies respiratoires dans une clinique vétérinaire associée à l'infection par le virus parainfluenza canin. Un groupe d'infections par le virus parainfluenza canin a été identifié dans une clinique vétérinaire spécialisée. Même si des éclosions d'infections par le virus parinfluenza canin n'ont pas été signalées antérieurement, les cliniques vétérinaires présentent certains des mêmes facteurs de risque qui peuvent être présents dans des lieux à risque traditionnellement élevé, comme les chenils. Par conséquent, il est nécessaire de considérer comme possible la transmission d'agents pathogènes respiratoires canins associée aux cliniques.(Traduit par Isabelle Vallières).

Impact on canine neutrophil preservation with the addition of bovine serum albumin to K3 -EDTA whole blood samples.

BACKGROUND: Cellular deterioration occurs with blood sample aging, impacting white blood cell (WBC) identification and differential accuracy. This may be exacerbated in samples from patients experiencing inflammation. Previously, bovine serum albumin (BSA) has been shown to improve cellular preservation of blood and other samples, but the effect on cell preservation in canine blood has not been assessed. OBJECTIVES: We aimed to determine the effects of BSA on neutrophil nuclear area when added to potassium ethylene diamine tetra-acetic acid (K3 -EDTA)-anticoagulated canine blood prior to blood smear preparation. We evaluated the impact of inflammatory leukograms, sample storage temperatures (4° and 20°C), and time on outcomes. MATERIALS AND METHODS: Canine K3 -EDTA-anticoagulated blood samples stored at 4° and 20°C were used from unique patients, 10 with and 10 without inflammatory leukograms. Blood smears were prepared from aliquots with or without the addition of 22% BSA at 0, 4, 8, 24, 48, and 72 h. The nuclear area was measured for 25 randomly selected neutrophils per slide using Fiji software. Mixed-effect linear regression modeling was performed (significance: P < 0.05). RESULTS: Nuclear area increased over time with and without added BSA. Both sample storage temperatures and the presence or absence of an inflammatory leukogram significantly impacted neutrophil nuclear area. Samples with added BSA had slightly higher predicted nuclear areas than those without BSA, but this difference was not statistically significant. CONCLUSIONS: BSA did not significantly impact neutrophil nuclear area and did not improve neutrophil preservation in canine blood samples.

Infrequent intrahousehold transmission of Clostridioides difficile between pet owners and their pets.

Companion animals have been shown to carry Clostridioides difficile strains that are similar or identical to strains found in people, and a small number of studies have shown that pets carry genetically identical C. difficile isolates as their owners, suggesting inter-species transmission. However, the directionality of transmission is ultimately unknown, and the frequency with which animals acquire C. difficile following their owners' infection is unclear. The goal of this study was to assess how often pets belonging to people with C. difficile infection carry genetically related C. difficile isolates. We enrolled pet owners from two medical institutions (University of Pennsylvania Health System (UPHS) and The Ohio State University Wexner Medical Center (OSUWMC)) who had diarrhoea with or without positive C. difficile assays and tested their faeces and their pets' faeces for C. difficile using both anaerobic culture and PCR assays. When microorganisms were obtained from both the owner and pet and had the same toxin profile or ribotype, isolates underwent genomic sequencing. Faecal samples were obtained from a total of 59 humans, 72 dogs and 9 cats, representing 47 complete households (i.e. where a sample was available from the owner and at least one pet). Of these, C. difficile was detected in 30 humans, 10 dogs and 0 cats. There were only two households where C. difficile was detected in both the owner and pet. In one of these households, the C. difficile isolates were of different toxin profiles/ribotypes (A+/B+ / RT 499 from the owner, A-/B- / RT PR22386 from the dog). In the other household, the isolates were genetically identical (one SNP difference). Interestingly, the dog from this household had recently received a course of antibiotics (cefpodoxime and metronidazole). Our findings suggest that inter-species transmission of C. difficile occurs infrequently in households with human C. difficile infections.

Household knowledge, attitudes and practices related to pet contact and associated zoonoses in Ontario, Canada.

BACKGROUND: Many human infections are transmitted through contact with animals (zoonoses), including household pets. Although pet ownership is common in most countries and non-pet owners may have frequent contact with pets, there is limited knowledge of the public's pet contact practices and awareness of zoonotic disease risks from pets. The objective of this study was to characterize the general public's knowledge, attitudes and risks related to pet ownership and animal contact in southern Ontario, Canada. METHODS: A self-administered questionnaire was distributed to individuals at two multi-physician clinics in Waterloo, Ontario, Canada during 2010. A single adult from each household was invited to participate in the study. RESULTS: Seventy five percent (641/853) of individuals approached completed the questionnaire. Pet ownership and contact were common; 64% of participants had a pet in their household and 37% of non-pet owning households had a member with at least weekly animal contact outside the home. Pet ownership was high (55%) for households with individuals at higher risk for infections (i.e., < 5 yrs, ≥ 65 yrs, immunocompromised). Most respondents (64%) indicated that they had never received information regarding pet-associated disease risks. When given a list of 11 infectious pathogens, respondents were only able to correctly classify just over half on their potential to be transmitted from pets to people (mean 6.4); independently, pet owners and those who recalled receiving information in the past about this topic were able to make significantly more correct identifications. Pet (36%) and non-pet owning households (10%) reported dog or cat bites or scratches during the preceding year. Households with individuals at higher risk for an infection did not differ from the remaining households regarding their perceived disease risk of pets, zoonotic disease knowledge, recall of being asked by their medical provider if they owned any pets, or recall of having received information regarding pet-associated disease risks and preventive measures. CONCLUSIONS: These results suggest that there is a need for accessible zoonotic disease information for both pet and non-owning households, with additional efforts made by veterinary, human and public health personnel. Immediate educational efforts directed toward households with individuals at higher risk to infections are especially needed.

Methicillin-resistant Staphylococcus aureus in a neonatal alpaca.

A 6-hour-old alpaca was presented for evaluation of respiratory difficulty. As part of routine surveillance, methicillin-resistant Staphylococcus aureus (MRSA) was identified from a nasal swab taken upon admission to the hospital. No signs of MRSA infection were noted. The MRSA strain recovered was a human epidemic clone that has been associated with horses. Methicillin-resistant S. aureus colonization can occur in camelids, and the potential animal and public health risks require consideration.