Dissecting the genetic basis of heterosis in elite super-hybrid rice.

Y900 is one of the top hybrid rice (Oryza sativa) varieties, with its yield exceeding 15 t·hm-2. To dissect the mechanism of heterosis, we sequenced the male parent line R900 and female parent line Y58S using long-read and Hi-C technology. High-quality reference genomes of 396.41 Mb and 398.24 Mb were obtained for R900 and Y58S, respectively. Genome-wide variations between the parents were systematically identified, including 1,367,758 single-nucleotide polymorphisms, 299,149 insertions/deletions, and 4,757 structural variations. The level of variation between Y58S and R900 was the lowest among the comparisons of Y58S with other rice genomes. More than 75% of genes exhibited variation between the two parents. Compared with other two-line hybrids sharing the same female parent, the portion of Geng/japonica (GJ)-type genetic components from different male parents increased with yield increasing in their corresponding hybrids. Transcriptome analysis revealed that the partial dominance effect was the main genetic effect that constituted the heterosis of Y900. In the hybrid, both alleles from the two parents were expressed, and their expression patterns were dynamically regulated in different tissues. The cis-regulation was dominant for young panicle tissues, while trans-regulation was more common in leaf tissues. Overdominance was surprisingly prevalent in stems and more likely regulated by the trans-regulation mechanism. Additionally, R900 contained many excellent GJ haplotypes, such as NARROW LEAF1, Oryza sativa SQUAMOSA PROMOTER BINDING PROTEIN-LIKE13, and Grain number, plant height, and heading date8, making it a good complement to Y58S. The fine-tuned mechanism of heterosis involves genome-wide variation, GJ introgression, key functional genes, and dynamic gene/allele expression and regulation pattern changes in different tissues and growth stages.

Applications of optical property measurement for quality evaluation of agri-food products: a review.

Spectroscopic techniques coupled with chemometric approaches have been widely used for quality evaluation of agricultural and food (agri-food) products due to the nondestructive, simple, fast, and easy characters. However, these techniques face the issues or challenges of relatively weak robustness, generalizability, and applicability in modeling and prediction because they measure the aggregate amount of light interaction with tissues, resulting in the combined effect of absorption and scattering of photons. Optical property measurement could separate absorption from scattering, providing new insights into more reliable prediction performance in quality evaluation, which is attracting increasing attention. In this review, a brief overview of the currently popular measurement techniques, in terms of light transfer principles and data analysis algorithms, is first presented. Then, the emphases are put on the recent advances of these techniques for measuring optical properties of agri-food products since 2000. Corresponding applications on qualitative and quantitative analyses of quality evaluation, as well as light transfer simulations within tissues, were reviewed. Furthermore, the leading groups working on optical property measurement worldwide are highlighted, which is the first summary to the best of our knowledge. Finally, challenges for optical property measurement are discussed, and some viewpoints on future research directions are also given.

HighlightsEmerging techniques for measuring optical properties are briefly introducedQualitative analyses of maturity evaluation and defect detection are reviewedQuantitative analyses of attribute prediction and microstructure estimation are presentedLight transfer simulations based on optical properties are comprehensively discussedLeading groups are summarized for the first time, to the best of our knowledgeChallenges and prospects for optical property measurement are given.

Photoredox-catalyzed trifluoromethylation of 2H-indazoles using TT-CF3+OTf- in ionic liquids.

A protocol for metal and oxidant free photoredox catalyzed trifluoromethylation of 2H-indazoles was developed by using Eosin Y as the photocatalyst and recoverable ionic liquids as the solvents. A series of trifluoromethylated products were obtained in moderate to good yields in this protocol under mild conditions. The reaction proceeded via a free-radical mechanism with a broad substrate range, excellent regioselectivity, and good functional group tolerance. Furthermore, the utility of this protocol was demonstrated by the synthesis of a highly selective ligand for estrogen receptor beta (ERß) and the drug granisetron. The protocol provides a mild and environmentally friendly solution for trifluoromethylation reaction.

Novel Salinity-Tolerant Third-Generation Hybrid Rice Developed via CRISPR/Cas9-Mediated Gene Editing.

Climate change has caused high salinity in many fields, particularly in the mud flats in coastal regions. The resulting salinity has become one of the most significant abiotic stresses affecting the world's rice crop productivity. Developing elite cultivars with novel salinity-tolerance traits is regarded as the most cost-effective and environmentally friendly approach for utilizing saline-alkali land. To develop a highly efficient green strategy and create novel rice germplasms for salt-tolerant rice breeding, this study aimed to improve rice salinity tolerance by combining targeted CRISPR/Cas9-mediated editing of the OsRR22 gene with heterosis utilization. The novel alleles of the genic male-sterility (GMS) and elite restorer line (733Srr22-T1447-1 and HZrr22-T1349-3) produced 110 and 1 bp deletions at the third exon of OsRR22 and conferred a high level of salinity tolerance. Homozygous transgene-free progeny were identified via segregation in the T2 generation, with osrr22 showing similar agronomic performance to wild-type (733S and HZ). Furthermore, these two osrr22 lines were used to develop a new promising third-generation hybrid rice line with novel salinity tolerance. Overall, the results demonstrate that combining CRISPR/Cas9 targeted gene editing with the "third-generation hybrid rice system" approach allows for the efficient development of novel hybrid rice varieties that exhibit a high level of salinity tolerance, thereby ensuring improved cultivar stability and enhanced rice productivity.

Allelic Diversification of the Wx and ALK Loci in Indica Restorer Lines and Their Utilisation in Hybrid Rice Breeding in China over the Last 50 Years.

Hybrid rice technology has been used for more than 50 years, and eating and cooking quality (ECQ) has been a major focus throughout this period. Waxy (Wx) and alkaline denaturation (ALK) genes have received attention owing to their pivotal roles in determining rice characteristics. However, despite significant effort, the ECQ of restorer lines (RLs) has changed very little. By contrast, obvious changes have been seen in inbred rice varieties (IRVs), and the ECQ of IRVs is influenced by Wx, which reduces the proportion of Wxa and increases the proportion of Wxb, leading to a decrease in amylose content (AC) and an increase in ECQ. Meanwhile, ALK is not selected in the same way. We investigated Wx alleles and AC values of sterile lines of female parents with the main mating combinations in widely used areas. The results show that almost all sterile lines were Wxa-type with a high AC, which may explain the low ECQ of hybrid rice. Analysis of hybrid rice varieties and RLs in the last 5 years revealed serious homogenisation among hybrid rice varieties.

A visible-light-induced cascade reaction of etherification/C-C cyclization: efficient synthesis of dibenzo[b,d]oxepin-7(6H)-ones.

A visible-light-induced palladium-catalyzed cascade reaction was developed by etherification/C-C coupling cyclization of α-bromoacetophenones with phenols. A series of dibenzo[b,d]oxepin-7(6H)-one derivatives were efficiently synthesized by using this method in good yields. Furthermore, this method was applied to the synthesis of protosappanin A. The protocol has advantages such as simple reaction conditions, wide range of substrates and high reaction efficiency.

Visible-Light-Induced Decarboxylation Coupling/Intramolecular Cyclization: A One-Pot Synthesis for 4-Aryl-2-quinolinone Derivatives.

A visible-light-induced decarboxylation coupling/intramolecular cyclization is reported. The one-pot synthesis system provides mild, efficient, and atom economical access to the synthesis of 4-aryl-2-quinolinone derivatives. It is notable that the necessary oxidant in the traditional decarboxylation coupling is replaced by the visible-light irradiation in this paper. In addition, the HBV inhibitor is synthesized by the one-pot synthesis system in an atom economical manner.

Synthesis of 8-azaprotosappanin A derivatives via intramolecular palladium-catalyzed ortho C-H activation/C-C cyclization and their antibacterial activity.

A novel synthetic protocol for the construction of eight-membered heterocycles by intramolecular palladium-catalyzed ortho C-H activation/C-C cyclization was proposed. With protosappanin A as the lead compound, 25 derivatives of 8-azaprotosappanin A were prepared in good yields by this protocol. Besides, a plausible reaction mechanism of the intramolecular cyclization was proposed. This strategy could be widely used in the synthesis of some natural products and drugs with large heterocycles due to the fast reaction rate and the mild conditions. In vitro antimicrobial activities of the synthesized compounds were assessed against the strains of Gram-positive bacteria and linezolid and ciprofloxacin were selected as the standard drugs. Some of the synthesized compounds were found to have excellent antibacterial activities.

ZNT1 Regulates the Proliferation, Migration and Invasion of HaCaT Cells Infected with HPV Through the PI3K/Akt Pathway.

Background: Condyloma acuminatum (CA), which is a highly contagious sexually transmitted illness generated by human papillomavirus (HPV) infection, is characterized by abnormal proliferation of keratinocytes resulting in verrucous lesions. Although solute carrier family 30 member 1 (ZNT1) is highly expressed in CA tissues, the function of ZNT1 in CA remains unclear. Methods: HPV transfection was performed in HaCaT to simulate the CA pathological environment. The mRNA and protein levels were monitored using RT-qPCR and immunoblotting. Cell viability was found using the MTT test. Cell invasion and migration were probed using the transwell and wound healing. Results: ZNT1 expression was up-regulated in CA tissues, and HPV transfection increased the expression of ZNT1. Overexpression of ZNT1 promoted the proliferation, migration and invasion of Human immortalized keratinocyte (HaCaT) transfected with HPV. Meanwhile, ZNT1 knockdown repressed the proliferation, migration and invasion of HaCaT that HPV transfected. Further research displayed that ZNT1 promoted the proliferation, migration and invasion of HaCaT transfected with HPV through the PI3K/Akt pathway. Conclusion: Our research confirmed that ZNT1 regulated the proliferation, migration and invasion of HaCaT transfected with HPV through the PI3K/Akt pathway, providing a new target for the effective remedy of CA.

Integrating deep learning and data fusion for enhanced oranges soluble solids content prediction using machine vision and Vis/NIR spectroscopy.

The visible/near infrared (Vis/NIR) spectrum will become distorted due to variations in sample color, thereby reducing the prediction accuracy of fruit composition. In this study, we aimed to develop a deep learning model with color correction capability to predict oranges soluble solids content (SSC) based on multi-source data fusion. Initially, a machine vision and Vis/NIR spectroscopy online acquisition device was designed to collect and analyze color images and transmission spectra. Subsequently, data fusion methods were proposed for color features and spectral data. Finally, color-correction one-dimensional convolutional neural network (1D-CNN) models base on multi-source data were constructed. The results showed that, the RMSEP of optimal color-correction model was decreased by 36.4 % and 16.1 % compared to partial least squares model and conventional 1D-CNN model, respectively. The multi-source data fusion of machine vision and Vis/NIR spectroscopy has the potential to improve the accuracy of food composition prediction.

Functional connectivity density aberrance in type 2 diabetes mellitus with and without mild cognitive impairment.

Purpose: The objective of this study was to investigate alterations in functional connectivity density (FCD) mapping and their impact on functional connectivity (FC) among individuals diagnosed with Type 2 diabetes mellitus (T2DM) across different cognitive states. Moreover, the study sought to explore the potential association between aberrant FCD/FC patterns and clinical or cognitive variables. Methods: A total of 211 participants were recruited for this study, consisting of 75 healthy controls (HCs), 89 T2DM patients with normal cognitive function (DMCN), and 47 T2DM patients with mild cognitive impairment (DMCI). The study employed FCD analysis to pinpoint brain regions exhibiting significant FCD alterations. Subsequently, these regions showing abnormal FCD served as seeds for FC analysis. Exploratory partial correlations were conducted to explore the relationship between clinical biochemical indicators, neuropsychological test scores, and altered FCD or FC. Results: The FCD analysis revealed an increased trend in global FCD (gFCD), local FCD (lFCD), and long-range FCD (lrFCD) within the bilateral supramarginal gyrus (SMG) among individuals with DMCN. Additionally, significant lFCD alterations were observed in the right inferior frontal gyrus and left precuneus when comparing DMCN to HCs and DMCI. Conclusion: When comparing individuals with T2DM and healthy controls (HCs), it was revealed that DMCN exhibited significant improvements in FCD. This suggests that the brain may employ specific compensatory mechanisms to maintain normal cognitive function at this stage. Our findings provide a novel perspective on the neural mechanisms involved in cognitive decline associated with T2DM.

Altered Subcortical Brain Volume and Cortical Thickness Related to Insulin Resistance in Type 2 Diabetes Mellitus.

PURPOSE: The objective of this study is to examine the alterations in subcortical brain volume and cortical thickness among individuals diagnosed with Type 2 diabetes mellitus (T2DM) through the application of morphometry techniques and, additionally, to investigate the potential association between these modifications and insulin resistance (IR). MATERIALS AND METHODS: The present cross-sectional study comprised a total of 121 participants (n = 48 with healthy controls [HCs] and n = 73 with T2DM) who were recruited and underwent a battery of cognitive testing and structural magnetic resonance imaging (MRI). FreeSurfer was used to process the MRI data. Analysis of covariance compared discrepancies in cortical thickness and subcortical brain volume between T2DM and HCs, adjusting for the potential confounding effects of gender, age, education, and body mass index (BMI). Exploratory partial correlations investigated links between IR and brain structure in T2DM participants. RESULTS: Compared with HCs, individuals with T2DM demonstrated a cortical thickness decrease in the right caudal middle frontal gyrus, right pars opercularis, left precentral gyrus, and bilateral superior frontal gyrus. Furthermore, this study for T2DM found that the severity of IR was inversely related to the volume of the left putamen and left hippocampus, as well as the thickness of the left pars orbitalis, left pericalcarine, right entorhinal area, and right rostral anterior cingulate gyrus. CONCLUSION: The evidence for structural brain changes in T2DM was observed, and alterations in cortical thickness were concentrated in the frontal lobes. Correlations between IR and frontal cortical thinning may serve as a potential neuroimaging marker of T2DM and lead to various diabetes-related brain complications.

Crystal structure and highly luminescent properties studies of bis-ß-diketonate lanthanide complexes.

A new bis(ß-diketonate), 1,3-bis(4,4,4-trifluoro-1,3-dioxobutyl)phenyl (BTP), which contains a trifluorinated alkyl group, has been prepared for the synthesis of two series of dinuclear lanthanide complexes with the general formula Ln2(BTP)3L2 [Ln(3+) = Eu(3+), L = DME(1), bpy(2), and phen(3); Ln(3+) = Sm(3+), L = DME(4), bpy(5), and phen(6); DME = ethylene glycol dimethyl ether, bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline]. The crystal structure of the free ligand has been determined and shows a twisted arrangement of the two binding sites around the 1,3-phenylene spacer. X-ray crystallographic analysis reveals that complexes 1, 2, 4, and 5 are triple-stranded dinuclear structures formed by three bis-bidentate ligands with two lanthanide ions. The room-temperature photoluminescence (PL) spectra of complexes 1-6 show that this bis-ß-diketonate can effectively sensitize rare earths (Sm(3+) and Eu(3+)) and produce characteristic emissions of the corresponding Eu(3+) and Sm(3+) ions. In addition, two bidentate nitrogen ancillary ligands, 2,2'-bipyridine (bpy) and 1,10-phenanthroline (phen), have been employed to enhance the luminescence quantum yields and lifetimes of both series of Eu(3+) and Sm(3+) complexes.

What drives horizontal logistics collaboration? A grounded theory analysis of Chinese logistics service providers.

Horizontal logistics collaboration is characterized by the highly professional cooperation of logistics service providers at the same level of the supply chain, which is an effective way to optimize the development and sustainability of modern logistics. Conducting a coding analysis of data from 41 in-depth interviews, this study constructs a theoretical framework of the driving force of horizontal logistics collaboration among Chinese logistics service providers to synchronously improve service, market, efficiency, and emergency competitiveness through cooperation with peer competitors to enhance sustainable comprehensive competitiveness. The four driving forces encourage logistics service providers to adjust their thoughts in the planning stage, change their behavior in the implementation stage, and transform their strategy in the evaluation stage. The results also indicate differences between China and other countries in the driving force, effective utilization of logistics workers, cross-institutional collaboration of state-owned enterprises, and integration of artificial intelligence technology. Finally, we propose relevant recommendations for managers.

N-Cyano-2,2'-biphenyldicarboimide as a Cyanation Reagent for Co(III)-Catalyzed C-H Cyanation of Indoles in Ionic Liquids.

A mild strategy for Co(III)-catalyzed C(sp2)-H cyanation of indoles was developed by using NCBLD as an electrophilic cyanation reagent and 1-butyl-3-acetylimidazole ditrifluoromethylsulfonimide ([BAIM]NTf2) as an environmentally friendly and recyclable solvent, and a series of 2-cyano products were obtained at room temperature. Adopting this strategy, the unnatural nucleotide fragment precursor of Remdesivir, which was a drug for COVID-19, was synthesized through cyano transformation, further proving the practicability of this cyanation method.

N-Trifluoromethyl Succinimide as a New Reagent for Direct C-H Trifluoromethylation of Free Anilines.

N-trifluoromethylsuccinimide (NTFS) as a new trifluoromethylation reagent was designed and prepared via Ag-CF3 , and applied to the direct trifluoromethylation of free aniline, and a series of trifluoromethyl products were obtained with good yields. The practicability of the protocol was verified by a gram-level experiment and the synthesis of the antiasthmatic drug Mabuterol. In addition, a possible radical mechanism was proposed and verified by related experiments. The protocol provided a new solution for C-H trifluoromethylation of free anilines.

Systemic lupus erythematosus Association between Osteomyelitis: A two-sample Mendelian randomization study in European population.

Background: Systemic lupus erythematosus (SLE) has been correlated with osteomyelitis (OM), yet the underlying causal relationship remains poorly understood. This study aims to investigate the causal association between SLE and OM using Mendelian randomization (MR) analysis. Methods: Genetic instrumental variables (IVs) correlated with SLE were extracted from a comprehensive genome-wide association study (GWAS) summary database (5201 cases and 9066 controls). OM was considered a SLE phenotype, and summary data from the fast GWA data portal were utilized for the analysis. Eligible IVs were extracted following rigorous quality control measures (P < 5 × 10-8, LD r2>0.001, distance 1 Mb, and F > 10). MR analysis was conducted using the Inverse Variance Weighted (IVW), MR-Egger, and Weighted Median (WM) methods after excluding potential confounders. Cochran's Q was applied for heterogeneity test. Pleiotropy was evaluated through MR-Egger intercept, MR-Pleiotropy Residual Sum and Outlier (MR-PRESSO) method, and Leave-one-SNP-out analysis. Result: A total of 40 eligible IVs were included for MR analysis. IVW results demonstrated a positive causal association between SLE and OM (P = 0.049, OR = 1.167). Heterogeneity analysis reveal no significant heterogeneity in the IVW analysis (P = 0.5503). Pleiotropy tests, including MR-PRESSO global test and MR-Egger intercept, indicated no evidence of pleiotropy in our findings (P > 0.05). Additionally, the Leave-one-SNP-out analysis showed no substantial deviations when removing individual SNPs, thus supporting the robustness of our results. Conclusion: This study establishes a genetic causal relationship between SLE and OM, indicating an increased risk of developing OM in individuals with SLE. Therefore, proactive management of SLE is advised to mitigate the risk of developing OM.

Analysis of Light Penetration Depth in Apple Tissues by Depth-Resolved Spatial-Frequency Domain Imaging.

Spatial-frequency domain imaging (SFDI) has been developed as an emerging modality for detecting early-stage bruises of fruits, such as apples, due to its unique advantage of a depth-resolved imaging feature. This paper presents theoretical and experimental analyses to determine the light penetration depth in apple tissues under spatially modulated illumination. Simulation and practical experiments were then carried out to explore the maximum light penetration depths in 'Golden Delicious' apples. Then, apple experiments for early-stage bruise detection using the estimated reduced scattering coefficient mapping were conducted to validate the results of light penetration depths. The results showed that the simulations produced comparable or a little larger light penetration depth in apple tissues (~2.2 mm) than the practical experiment (~1.8 mm or ~2.3 mm). Apple peel further decreased the light penetration depth due to the high absorption properties of pigment contents. Apple bruises located beneath the surface peel with the depth of about 0-1.2 mm could be effectively detected by the SFDI technique. This study, to our knowledge, made the first effort to investigate the light penetration depth in apple tissues by SFDI, which would provide useful information for enhanced detection of early-stage apple bruising by selecting the appropriate spatial frequency.

2,9,10-Trimeth-oxy-dibenzo[b,d]oxepin-7(6H)-one.

The title compound, C(17)H(16)O(5), was prepared through a cyclization reaction of 2-(3',4',5-trimeth-oxy-biphenyl-2-yl-oxy)acetyl chloride. The two benzene rings form a dihedral angle of 34.55 (5)°. The crystal structure does not feature any hydrogen bonds.