Fabrication of multilevel metalenses using multiphoton lithography: from design to evaluation.

We present a procedure for the design of multilevel metalenses and their fabrication with multiphoton-based direct laser writing. This work pushes this fast and versatile fabrication technique to its limits in terms of achievable feature size dimensions for the creation of compact high-numerical aperture metalenses on flat substrates and optical fiber tips. We demonstrate the design of metalenses with various numerical apertures up to 0.96, and optimize the fabrication process towards nanostructure shape reproducibility. We perform optical characterization of the metalenses towards spot size, focusing efficiency, and optical functionality with a fiber beam collimation design, and compare their performance with refractive and diffractive counterparts fabricated with the same technology.

Wide-Field-of-View Multispectral Camera Design for Continuous Turfgrass Monitoring.

Sustainably using resources, while reducing the use of chemicals, is of major importance in agriculture, including turfgrass monitoring. Today, crop monitoring often uses camera-based drone sensing, offering an accurate evaluation but typically requiring a technical operator. To enable autonomous and continuous monitoring, we propose a novel five-channel multispectral camera design suitable for integrating it inside lighting fixtures and enabling the sensing of a multitude of vegetation indices by covering visible, near-infrared and thermal wavelength bands. To limit the number of cameras, and in contrast to the drone-sensing systems that show a small field of view, a novel wide-field-of-view imaging design is proposed, featuring a field of view exceeding 164°. This paper presents the development of the five-channel wide-field-of-view imaging design, starting from the optimization of the design parameters and moving toward a demonstrator setup and optical characterization. All imaging channels show an excellent image quality, indicated by an MTF exceeding 0.5 at a spatial frequency of 72 lp/mm for the visible and near-infrared imaging designs and 27 lp/mm for the thermal channel. Consequently, we believe our novel five-channel imaging design paves the way toward autonomous crop monitoring while optimizing resource usage.

Miniaturized cost-effective broadband spectrometer employing a deconvolution reconstruction algorithm for resolution enhancement.

We demonstrate a miniaturized broadband spectrometer employing a reconstruction algorithm for resolution enhancement. We use an opto-digital co-design approach, by firstly designing an optical system with certain residual aberrations and then correcting these aberrations with a digital algorithm. The proposed optical design provides an optical resolution less than 1.7 nm in the VIS-channel (400-790 nm) and less than 3.4 nm in the NIR-channel (760-1520 nm). Tolerance analysis results show that the components are within a commercial class, ensuring a cost-efficient design. We build the prototype with a size of 37x30x26 mm3 and demonstrate that by applying a restoration algorithm, the optical resolution can be further improved to less than 1.3 nm (VIS-channel) and less than 2.3 nm (NIR-channel).

Design and demonstration of a six-channel multiresolution imaging system.

Multichannel imaging systems consist of multiple channels that have different imaging characteristics (fields-of-view and angular resolutions). We design and demonstrate a six-channel multiresolution imaging system that can achieve a relative magnification ratio of up to 10 times between the channels and at the same time result in different depths-of-field. The imaging system consists of two double-sided lens arrays made of PMMA material, a baffle to eliminate possible cross talk between neighboring channels, and a Sony full-frame image sensor. The imaging system was tested by capturing images of stationary and moving objects. The acquired images exhibit different resolutions, fields-of-view, and levels of blur proving our concept.

Freeform Wide Field-of-View Spaceborne Imaging Telescope: From Design to Demonstrator.

Wide field-of-view imaging optics offer a huge potential for space-based Earth observation enabling the capture of global data. Reflective imaging telescopes are often favored, as they do not show chromatic aberrations and are less susceptible to radiation darkening than their refractive counterparts. However, the main drawback of reflective telescopes is that they are limited with respect to field-of-view while featuring large dimensions. We propose the use of freeform optics to maximize the field of view while maintaining diffraction-limited image quality and minimizing system dimensions. In this paper, we present a novel freeform wide field-of-view reflective telescope, starting from the optical design, and continuing to tolerancing analysis and manufacture, towards a proof-of-concept demonstrator. The novel telescope features a full field-of-view of 120° while showing an exceptional spatial resolution of 2.6 km and fitting within 1 CubeSat unit. To the best of our knowledge, this is the widest field-of-view that has ever been realized for a space-based telescope, nearly reaching Earth observation from limb to limb from an altitude of about 700 km. We hope this design paves the way for future space missions enabling improved Earth observation and leading to enhanced monitoring of climate and climate change.

Automated freeform imaging system design with generalized ray tracing and simultaneous multi-surface analytic calculation.

Recently, freeform optics has been widely used due to its unprecedented compactness and high performance, especially in the reflective designs for broad-wavelength imaging applications. Here, we present a generalized differentiable ray tracing approach suitable for most optical surfaces. The established automated freeform design framework simultaneously calculates multi-surface coefficients with merely the system geometry known, very fast for generating abundant feasible starting points. In addition, we provide a "double-pass surface" strategy with desired overlap (not mutually centered) that enables a component reduction for very compact yet high-performing designs. The effectiveness of the method is firstly demonstrated by designing a wide field-of-view, fast f-number, four-mirror freeform telescope. Another example shows a two-freeform, three-mirror, four-reflection design with high compactness and cost-friendly considerations with a double-pass spherical mirror. The present work provides a robust design scheme for reflective freeform imaging systems in general, and it unlocks a series of new 'double-pass surface' designs for very compact, high-performing freeform imaging systems.

Miniature freeform flow-line lightguide for sensing: from design to fabrication.

We present the development of a compact (about 1.3 × 2.0 × 20 mm3) freeform optical lightguide for sensing applications, from the conceptual design to the fabrication through injection molding. The design of the optic is based on the flow-line method from Nonimaging Optics, which allows the desired optical functionalities (45° half-acceptance and 40° beam steering) while meeting particularly tight mechanical and geometrical constraints. An extensive analysis of the effects of fabrication parameters on the performances demonstrates the importance of minimizing the fillet radius. This requisite inspired a special procedure for designing the mold, which is built as a "3D puzzle" assembly of separate pieces, each one dedicated to one specific side surface of the lightguide. This technique enables uniform optical quality on all the optic's surfaces and removes the need of a fillet radius in the mold. At present, the first lightguide prototypes have been fabricated; after the coating phase, they will be ready for the validation stage.

Increasing the Microfabrication Performance of Synthetic Hydrogel Precursors through Molecular Design.

Implementation of hydrogel precursors in two-photon polymerization (2PP) technology provides promising opportunities in the tissue engineering field thanks to their soft characteristics and similarity to extracellular matrix. Most of the hydrogels, however, are prone to post-fabrication deformations, leading to a mismatch between the computer-aided design and the printed structure. In the present work, we have developed novel synthetic hydrogel precursors to overcome the limitations associated with 2PP processing of conventional hydrogel precursors such as post-processing deformations and a narrow processing window. The precursors are based on a poly(ethylene glycol) backbone containing urethane linkers and are, on average, functionalized with six acrylate terminal groups (three on each terminal group). As a benchmark material, we exploited a precursor with an identical backbone and urethane linkers, albeit functionalized with two acrylate groups, that were reported as state-of-the-art. An in-depth characterization of the hexafunctional precursors revealed a reduced swelling ratio (<0.7) and higher stiffness (>36 MPa Young's modulus) compared to their difunctional analogs. The superior physical properties of the newly developed hydrogels lead to 2PP-based fabrication of stable microstructures with excellent shape fidelity at laser scanning speeds up to at least 90 mm s-1, in contrast with the distorted structures of conventional difunctional precursors. The hydrogel films and microscaffolds revealed a good cell interactivity after functionalization of their surface with a gelatin methacrylamide-based coating. The proposed synthesis strategy provides a one-pot and scalable synthesis of hydrogel building blocks that can overcome the current limitations associated with 2PP fabrication of hydrogel microstructures.

Monitoring of Torque Induced Strain in Composite Shafts with Embedded and Surface-Mounted Optical Fiber Bragg Gratings.

In this study, silica glass, optical fiber Bragg gratings (FBGs) are used for torque-induced strain monitoring in carbon fiber reinforced polymer (CFRP) hollow shafts toward the development of a methodology for structural load monitoring. Optical fibers with gratings are embedded during shaft manufacturing, by an industrial filament winding process, along different orientations with respect to its central axis and surface mounted after production. Experimental results are supported by numerical modeling of the shaft with appropriate boundary conditions and homogenized material properties. For an applied torque up to 800 Nm, the strain sensitivity of an embedded grating positioned along the reinforcing fibers' direction winded under 55° is in the order of 3.6 pm/Nm, while this value is more than 4× times higher than the other examined orientations. The study also shows that surface-mounted optical fiber Bragg gratings along the reinforcing carbon fibers' direction perform equally well in monitoring strains in composite shafts under torque.

Compact conical beam shaper and freeform segmented reflector for SERS analysis.

We present a Raman spectroscopy setup containing a conical beam shaper in combination with a freeform segmented reflector for surface enhanced Raman scattering (SERS) analysis. The freeform segmented reflector and the conical beam shaper are designed by numerical approaches and fabricated by means of ultra-precision diamond tooling. The segmented reflector has a numerical aperture of 0.984 and a working distance of 1mm for SERS measurements. We perform systematic simulations using non-sequential ray tracing to assess the detecting abilities of the designed SERS-based system. We implement a proof-of-concept setup and demonstrate the confocal behavior by measuring the SERS signal of 10µM rhodamine B solution. The experimental results agree well with the simulations concerning the misalignment tolerances of the beam shaper with respect to the segmented reflector and the misalignment tolerances of the collecting fiber. In addition, we conduct benchmark SERS measurements by using a 60× objective lens with a numerical aperture of 0.85. We find that the main Raman intensity of rhodamine B at 1502 cm-1 obtained by our segmented reflector working together with the conical beam shaper is approximately 30% higher compared to the commercial objective lens.

3D direct laser writing of microstructured optical fiber tapers on single-mode fibers for mode-field conversion.

We present a design and fabrication approach for 3D printed polymer microstructured optical fiber tapers on standard single-mode glass fibers for efficient and compact mode-field conversion. This paves the way towards complex functionalized fiber tips for various applications, like sensors and beam shaping components, currently limited by the mode-field size and distribution of standard optical fibers. In this paper, we demonstrate the potential of mode-field converting tapers for relaxing the misalignment tolerance in fiber-to-fiber connections and maximizing the coupling efficiency in fiber-to-chip connections. We demonstrate a mode-field diameter expansion ratio of 1.7 and reduction ratio of 3 and show that our microstructured tapers achieve a comparable performance in coupling efficiency as their step-index counterparts, while providing greater robustness.

High-Resolution 3D Bioprinting of Photo-Cross-linkable Recombinant Collagen to Serve Tissue Engineering Applications.

Various biopolymers, including gelatin, have already been applied to serve a plethora of tissue engineering purposes. However, substantial concerns have arisen related to the safety and the reproducibility of these materials due to their animal origin and the risk associated with pathogen transmission as well as batch-to-batch variations. Therefore, researchers have been focusing their attention toward recombinant materials that can be produced in a laboratory with full reproducibility and can be designed according to specific needs (e.g., by introducing additional RGD sequences). In the present study, a recombinant protein based on collagen type I (RCPhC1) was functionalized with photo-cross-linkable methacrylamide (RCPhC1-MA), norbornene (RCPhC1-NB), or thiol (RCPhC1-SH) functionalities to enable high-resolution 3D printing via two-photon polymerization (2PP). The results indicated a clear difference in 2PP processing capabilities between the chain-growth-polymerized RCPhC1-MA and the step-growth-polymerized RCPhC1-NB/SH. More specifically, reduced swelling-related deformations resulting in a superior CAD-CAM mimicry were obtained for the RCPhC1-NB/SH hydrogels. In addition, RCPhC1-NB/SH allowed the processing of the material in the presence of adipose tissue-derived stem cells that survived the encapsulation process and also were able to proliferate when embedded in the printed structures. As a consequence, it is the first time that successful HD bioprinting with cell encapsulation is reported for recombinant hydrogel bioinks. Therefore, these results can be a stepping stone toward various tissue engineering applications.

A Tunable Freeform-Segmented Reflector in a Microfluidic System for Conventional and Surface-Enhanced Raman Spectroscopy.

We present a freeform-segmented reflector-based microfluidic system for conventional Raman and Surface-Enhanced Raman Scattering (SERS) analysis. The segmented reflector is directly designed by a numerical approach. The polymer-based Raman system strongly suppresses the undesirable background because it enables confocal detection of Raman scattering through the combination of a freeform reflector and a microfluidic chip. We perform systematic simulations using non-sequential ray tracing with the Henyey-Greenstein model to assess the Raman scattering behavior of the substance under test. We fabricate the freeform reflector and the microfluidic chip by means of ultra-precision diamond turning and laser cutting respectively. We demonstrate the confocal behavior by measuring the Raman spectrum of ethanol. Besides, we calibrate the setup by performing Raman measurements on urea and potassium nitrate solutions with different concentrations. The detection limit of our microfluidic system is approximately 20 mM according to the experiment. Finally, we implement a SERS microfluidic chip and discriminate 100 µM urea and potassium nitrate solutions.

Technological advancements for the development of stem cell-based models for hepatotoxicity testing.

Stem cells are characterized by their self-renewal capacity and their ability to differentiate into multiple cell types of the human body. Using directed differentiation strategies, stem cells can now be converted into hepatocyte-like cells (HLCs) and therefore, represent a unique cell source for toxicological applications in vitro. However, the acquired hepatic functionality of stem cell-derived HLCs is still significantly inferior to primary human hepatocytes. One of the main reasons for this is that most in vitro models use traditional two-dimensional (2D) setups where the flat substrata cannot properly mimic the physiology of the human liver. Therefore, 2D-setups are progressively being replaced by more advanced culture systems, which attempt to replicate the natural liver microenvironment, in which stem cells can better differentiate towards HLCs. This review highlights the most recent cell culture systems, including scaffold-free and scaffold-based three-dimensional (3D) technologies and microfluidics that can be employed for culture and hepatic differentiation of stem cells intended for hepatotoxicity testing. These methodologies have shown to improve in vitro liver cell functionality according to the in vivo liver physiology and allow to establish stem cell-based hepatic in vitro platforms for the accurate evaluation of xenobiotics.

IR femtosecond pulsed laser-based fiber Bragg grating inscription in a photonic crystal fiber using a phase mask and a short focal length lens.

Fiber Bragg grating inscription with infrared femtosecond pulsed lasers in photonic crystal fiber is far from being trivial due to the presence of air holes in the cladding region and the non-linear nature of the absorption process inducing the required refractive index changes. We have studied this problem numerically and experimentally for a phase mask-based writing setup equipped with short focal length cylindrical lenses, which are often used for through-coating and high temperature stable grating writing. We have shown that for a cylindrical lens with a focal length f of 10 mm, the hexagonal lattice PCF needs to be translated away from the beam waist position by around 15 µm to efficiently deliver the energy to the core region. We have also investigated the importance of the PCF's angular orientation and we have shown that for some optimal positions the same behavior is observed for cylindrical lenses with different focal lengths. Finally, we have succeeded in writing a 4 dB strong grating in a photonic crystal fiber with a 1030 nm femtosecond pulsed laser in around 4 seconds, using an acylindrical lens with f = 10 mm.

Highly Reactive Thiol-Norbornene Photo-Click Hydrogels: Toward Improved Processability.

In the present work, gelatin type B is modified with highly reactive norbornene functionalities (Gel-NB) following a one-pot synthesis approach to enable subsequent thiol-ene photo-click crosslinking. The modification strategy displays close control over the amount of introduced functionalities. Additionally, Gel-NB exhibits considerably improved processing capabilities in terms of two-photon polymerization when benchmarked to earlier-reported crosslinkable gelatin derivatives (e.g., gelatin-methacrylamide (Gel-MOD) and gelatin-methacrylamide-aminoethylmethacrylate (Gel-MOD-AEMA)). The improvement is especially apparent in terms of minimally required laser power (20 mW vs ≥60 mW (Gel-MOD) vs ≥40 mW (Gel-MOD-AEMA) at 100 mm s-1 scan speed) and processable concentration range (≥5 w/v% vs ≥10 w/v% (Gel-MOD/Gel-MOD-AEMA)). Furthermore, the proposed functionalization scheme maintains the excellent biocompatibility and cell interactivity of gelatin. Additionally, the norbornene functionalities have potential for straightforward postprocessing "thiol-ene" surface grafting of active molecules. As a consequence, a very promising material toward tissue engineering applications and more specifically, biofabrication, is presented.

Compact illumination optic with three freeform surfaces for improved beam control.

Multi-chip and large size LEDs dominate the lighting market in developed countries these days. Nevertheless, a general optical design method to create prescribed intensity patterns for this type of extended sources does not exist. We present a design strategy in which the source and the target pattern are described by means of "edge wavefronts" of the system. The goal is then finding an optic coupling these wavefronts, which in the current work is a monolithic part comprising up to three freeform surfaces calculated with the simultaneous multiple surface (SMS) method. The resulting optic fully controls, for the first time, three freeform wavefronts, one more than previous SMS designs. Simulations with extended LEDs demonstrate improved intensity tailoring capabilities, confirming the effectiveness of our method and suggesting that enhanced performance features can be achieved by controlling additional wavefronts.

Opportunities for visible supercontinuum light generation in integrated diamond waveguides.

We numerically show the advantages of using diamond-on-insulator (DOI) waveguides to design compact supercontinuum (SC) light sources for the visible (VIS) wavelength range. We conclude that the DOI platform is more suitable than silicon nitride waveguides for tailoring the dispersion in such a way that a zero-dispersion wavelength (ZDW) is obtained in the VIS, as is required to achieve efficient VIS SC generation (SCG). After designing a DOI waveguide that features a ZDW at ∼600 nm, we exploit it to numerically obtain a smooth SC ranging from 453 nm to 1030 nm above the -30 dB point after propagation over 4 mm. Our result extends beyond the state-of-the-art shortest VIS wavelengths induced by SCG in integrated waveguides, while using ∼26 times lower input energy and a shorter waveguide length, thus showcasing the potential of the DOI platform for on-chip VIS SC light sources.

Polarization dynamics induced by parallel optical injection in a single-mode VCSEL.

We report an experimental study of the polarization nonlinear dynamics in a 1550 nm single-mode vertical-cavity surface-emitting laser (VCSEL) subject to parallel optical injection. Experimentally measured stability maps identifying regions of different nonlinear dynamics for various values of bias current are reported. We show that VCSELs with more than a 35 dB polarization mode suppression ratio can have rich nonlinear dynamics in both linear polarizations, including periodic and chaotic behaviors appearing simultaneously in both polarization modes.

Cross-Linkable Gelatins with Superior Mechanical Properties Through Carboxylic Acid Modification: Increasing the Two-Photon Polymerization Potential.

The present work reports on the development of photo-cross-linkable gelatins sufficiently versatile to overcome current biopolymer two-photon polymerization (2PP) processing limitations. To this end, both the primary amines as well as the carboxylic acids of gelatin type B were functionalized with photo-cross-linkable moieties (up to 1 mmol/g) resulting in superior and tunable mechanical properties (G' from 5000 to 147000 Pa) enabling efficient 2PP processing. The materials were characterized in depth prior to and after photoinduced cross-linking using fully functionalized gelatin-methacrylamide (gel-MOD) as a benchmark to assess the effect of functionalization on the protein properties, cross-linking efficiency, and mechanical properties. In addition, preliminary experiments on hydrogel films indicated excellent in vitro biocompatibility (close to 100% viability) both in the presence of MC3T3 preosteoblasts and L929 fibroblasts. Moreover, 2PP processing of the novel derivative was superior in terms of applied laser power (≥40 vs ≥60 mW for gel-MOD at 100 mm/s) as well as post-production swelling (0-20% vs 75-100% for gel-MOD) compared to those of gel-MOD. The reported novel gelatin derivative (gel-MOD-AEMA) proves to be extremely suitable for direct laser writing as both superior mimicry of the applied computer-aided design (CAD) was obtained while maintaining the desired cellular interactivity of the biopolymer. It can be anticipated that the present work will also be applicable to alternative biopolymers mimicking the extracellular environment such as collagen, elastin, and glycosaminoglycans, thereby expanding current material-related processing limitations in the tissue engineering field.