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1.
HIV Med ; 25(3): 332-342, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38012059

RESUMEN

BACKGROUND: The transition between inpatient and outpatient care for hospitalized people with HIV represents an opportunity for linkage and re-engagement in care. We evaluated whether attendance at a post-hospitalization visit ('discharge clinic') within 1-2 weeks of discharge would reduce readmissions and improve retention in care (RIC) among people with HIV in San Diego, California, USA. METHODS: This was a retrospective cohort study of people with HIV hospitalized between June 2020 and November 2021. Our primary outcome was 30-day readmissions among people with HIV who did or did not attend a discharge clinic visit. Secondary outcomes included the effect of discharge clinic attendance on RIC, along with the impact of attendance at any HIV clinic visit within 30 days of discharge on readmissions and RIC. RESULTS: We evaluated 114 people with HIV, of whom 77 (67.5%) and 90 (78.9%) attended a discharge clinic visit or any HIV clinic visit within 30 days of discharge, respectively. Active substance use disorder (SUD) was associated with failing to attend a discharge clinic visit (odds ratio 0.31; 95% confidence interval 0.13-0.77). We observed no significant differences in readmissions between people with HIV who did or did not attend a discharge clinic visit; however, the former had significantly higher 6-month RIC (79.2% vs. 35.1%, p < 0.001). People with HIV attending any HIV clinic visit within 30 days of discharge had significantly fewer 30-day readmissions (8.9% vs. 29.2%, p = 0.02) and better 6-month RIC (75.6% vs. 25%, p < 0.001) than those who did not attend. CONCLUSION: Early hospital follow-up care was associated with a reduction in readmissions among people with HIV. Active SUD was a significant barrier to linkage to outpatient follow-up and RIC.


Asunto(s)
Infecciones por VIH , Retención en el Cuidado , Humanos , Readmisión del Paciente , Alta del Paciente , Estudios de Seguimiento , Estudios Retrospectivos , Hospitales
2.
Subst Use Misuse ; 59(5): 699-706, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38170177

RESUMEN

BACKGROUND: Nicotine and tobacco product (NTP) and cannabis use are common in adolescence/young adulthood and increase risk for negative psychosocial outcomes. This study investigated associations among adolescent/young adults' initial experiences with NTPs, lifetime frequency of substance use, substance-related problems, and mental health symptoms. METHOD: Adolescents and young adults enrolled in a study on NTP and cannabis use were asked at what age they initiated the use of NTPs and were assigned to groups based on which product or substance(s) they reported using at the earliest age. Participants who reported use of NTPs (in isolation, without cannabis) first (N = 78, "NTP-only"), simultaneous use of NTPs and cannabis first (e.g., blunt or bowl; N = 25, "Simult-only"), use of both NTPs in isolation and simultaneous use at the same age (N = 48, "NTP + Simult"), and no NTP use (N = 53, "NTP-naïve") were compared on substance use, substance-related problems, and mental health symptoms. RESULTS: Groups differed on lifetime frequency of NTP, simultaneous, and cannabis use, with NTP users reporting more substance use episodes and substance-related problems than the NTP-naïve group. The lifetime frequency of cannabis use did not differ across NTP use groups. NTP use was associated with increased anxiety and depression, with no significant differences between groups. CONCLUSIONS: Adolescents and young adults who use nicotine may be at increased risk for greater nicotine use and mental health consequences, but initiating NTP use simultaneously with cannabis may not increase the risk of negative outcomes above and beyond nicotine initiation. Prospective longitudinal research is needed to establish temporal associations between first-used NTP/cannabis products and relevant outcomes.


Asunto(s)
Cannabis , Sistemas Electrónicos de Liberación de Nicotina , Alucinógenos , Fumar Marihuana , Trastornos Relacionados con Sustancias , Tabaquismo , Adulto Joven , Humanos , Adolescente , Adulto , Nicotina/efectos adversos , Fumar Marihuana/psicología , Estudios Prospectivos , Trastornos Relacionados con Sustancias/psicología , Tabaquismo/psicología , Productos de Tabaco
3.
Environ Geochem Health ; 45(2): 333-342, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-35246781

RESUMEN

Residents and advocacy groups began voicing concerns over the environmental quality located in the neighborhoods of Kashmere Gardens, Fifth Ward, and Denver Harbor in Houston, TX, following the confirmation of a cancer cluster in 2019 and another in 2021. These neighborhoods are in close proximity to a railyard and former wood treatment plant known to have utilized coal tar creosote and contain polycyclic aromatic hydrocarbons (PAHs). This research took core soil samples in September and October 2020 from 46 sites to assess for the presence and concentration of the U.S. Environmental Protection Agency's (USEPA) 7 Carcinogenic PAHs. Results showed the cumulative concentration of these PAHs in each sample was variable with a range of 13,767 ng/g to 328 ng/g and a mean of 2,517.2 ng/g ± 3122. A regional soil screening evaluation revealed that 40 of the 46 soil samples were in excess of the USEPAs most conservative screening levels of 1.0 × 10-6 increased cancer risk, but none exceeding levels considered actionable for remediation. This study is a fundamental first step for quantifying the environmental pollutants in this minority-majority community. Findings revealed a low risk of cancer risk based on current PAH concentrations alone but cannot assess contributions from other contaminants or from past, possibly higher, levels of contamination. Further research is needed to identify the potential casual pathways of the observed cancer cluster and to explore possible remediation needs.


Asunto(s)
Neoplasias , Hidrocarburos Policíclicos Aromáticos , Contaminantes del Suelo , Humanos , Suelo , Carbón Mineral/análisis , Contaminantes del Suelo/toxicidad , Contaminantes del Suelo/análisis , Monitoreo del Ambiente/métodos , Hidrocarburos Policíclicos Aromáticos/toxicidad , Hidrocarburos Policíclicos Aromáticos/análisis , Texas/epidemiología , Justicia Ambiental , Neoplasias/inducido químicamente , Neoplasias/epidemiología , Medición de Riesgo , China
4.
Int J Behav Nutr Phys Act ; 19(1): 113, 2022 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-36050778

RESUMEN

BACKGROUND: Food literacy is theorised to improve diet quality, nutrition behaviours, social connectedness and food security. The definition and conceptualisation by Vidgen & Gallegos, consisting of 11 theoretical components within the four domains of planning and managing, selecting, preparing and eating, is currently the most highly cited framework. However, a valid and reliable questionnaire is needed to comprehensively measure this conceptualisation. Therefore, this study draws on existing item pools to develop a comprehensive food literacy questionnaire using item response theory. METHODS: Five hundred Australian adults were recruited in Study 1 to refine a food literacy item pool using principal component analysis (PCA) and item response theory (IRT) which involved detailed item analysis on targeting, responsiveness, validity and reliability. Another 500 participants were recruited in Study 2 to replicate item analysis on validity and reliability on the refined item pool, and 250 of these participants re-completed the food literacy questionnaire to determine its test-retest reliability. RESULTS: The PCA saw the 171-item pool reduced to 100-items across 19 statistical components of food literacy. After the thresholds of 26 items were combined, responses to the food literacy questionnaire had ordered thresholds (targeting), acceptable item locations (< -0.01 to + 1.53) and appropriateness of the measurement model (n = 92% expected responses) (responsiveness), met outfit mean-squares MSQ (0.48-1.42) (validity) and had high person, item separation (> 0.99) and test-retest (ICC 2,1 0.55-0.88) scores (reliability). CONCLUSIONS: We developed a 100-item food literacy questionnaire, the IFLQ-19 to comprehensively address the Vidgen & Gallegos theoretical domains and components with good targeting, responsiveness, reliability and validity in a diverse sample of Australian adults.


Asunto(s)
Alimentos , Alfabetización en Salud , Adulto , Australia , Humanos , Psicometría , Reproducibilidad de los Resultados , Encuestas y Cuestionarios
5.
Public Health Nutr ; 25(11): 3235-3239, 2022 11.
Artículo en Inglés | MEDLINE | ID: mdl-35942634

RESUMEN

Our dominant food system is a primary driver of worsening human and planetary health. Held in March 2022, the Public Health Association of Australia's Food Futures Conference was an opportunity for people working across the food system to connect and advocate for a comprehensive, intersectoral, whole-of-society food and nutrition policy in Australia to attenuate these issues. Conference themes included food systems for local and global good; ecological nutrition; social mobilisation for planetary and public good; food sovereignty and food equity. Students and young professionals are integral in transforming food systems, yet they are under-represented in the academic workforce, across publishing, scientific societies and conference plenaries. A satellite event was held to platform initiatives from early career researchers (ECR) in areas integral for improving planetary and public good. The research topics discussed in this commentary reflect sub-themes of the conference under investigation by ECR: food systems governance and regulation; local food policies; commercial determinants of health; sustainable healthy diets; and food equity and sovereignty.


Asunto(s)
Política Nutricional , Salud Pública , Australia , Humanos
6.
Med Humanit ; 48(4): 421-430, 2022 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34759026

RESUMEN

Literary and medical historical scholars have long explored the work of physician-writers and the cross-pollination of literature and medicine. However, few scholars have considered how these interactions have shaped medical manuscripts and the echoes they contain of the emotional contours of the medical encounter. This essay uses the papers of Southern physician Andrew Bowles Holder (1860-1896) to explore how the emotions of the physician were managed at the bedside and in the aftermath of medical encounters through recourse to literary thinking. Holder, like many 19th-century physicians, was an avid reader with an interest in literary endeavours, and his manuscripts reveal the influences of literature on his work as a physician. This article frames the bedside as a theatre of emotions, in which Holder's performance and management of his emotions was key to his professional identity. His literary interests thus provided him with two tools: first, literature provided him with models for how to respond to and record different kinds of medical encounters, particularly deaths, near-death experiences and childbirth; second, his mode of keeping these records, which included the production of poetry as well as medical prose, served as a technology of coping, further allowing him to manage his emotions by exorcising them on the page.


Asunto(s)
Médicos , Escritura , Masculino , Humanos , Emociones
7.
Bull Hist Med ; 96(2): 182-210, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35912618

RESUMEN

While nineteenth-century regular physicians were expected to project a circumscribed affect in the exercise of their duties, they were not always successful in maintaining this performance. Archival sources, particularly the manuscript casebook for private practice, reveal the slippage between the performance of appropriate affect and the felt, interior emotions of the physician. This essay frames the casebook as an affective genre, building on Gianna Pomata's concept of epistemic genre. I argue that the nineteenth-century casebook, particularly when compared with the published case narrative, can reveal the disjunction or slippage between the expected performance of affect-that which Osler and others wished to prescribe for medical practitioners-and the felt reality or interiority of the physician. This article proposes the concept of affective genre and then explores its utility through close analysis of the casebook of a single practitioner, Andrew Bowles Holder, and selected examples from the casebooks of his contemporaries.


Asunto(s)
Médicos , Humanos
8.
Infection ; 49(6): 1203-1211, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-34368941

RESUMEN

PURPOSE: Overlapping clinical features of cutaneous leishmaniasis (CL) with ulcers caused by fungi and mycobacteria necessitate confirmatory diagnostic testing. We evaluated a handheld battery-operated device for detection of CL and common fungal and mycobacterial causes of ulcers. METHODS: We validated Palm PCR™ for detection of common ulcerative skin pathogens using ATCC® reference and clinical strains of Leishmania, mycobacteria, and fungi in the lab and field. Amplified products were Sanger sequenced. Performance characteristics were calculated using conventional PCR as a reference standard. RESULTS: Palm PCR™ detected 100% of ATCC® strains of Leishmania, fungi, and mycobacteria, with sensitivity and specificity of 90% and 91.7%, respectively. In the field, the sensitivity for detection of Leishmania in patients with suspected CL was 100%. In 61% of CL patients, co-colonization with genera such as Malassezia, Aspergillus, Candida, and Cladosporium was detected. In 50% of CL patients with an inflammatory (secondarily infected) phenotype, detected fungal species had known associations with human cutaneous disease. CONCLUSIONS: Palm PCR™ performs comparably to conventional PCR for detection of Leishmania, fungi, and mycobacteria. This work has implications for the diagnostic approach to tropical ulcers, and has the potential to improve field detection of ulcerative pathogens in resource constrained areas.


Asunto(s)
Leishmania , Leishmaniasis Cutánea , Mycobacterium , Hongos , Humanos , Leishmania/genética , Leishmaniasis Cutánea/diagnóstico , Perú , Sistemas de Atención de Punto , Sensibilidad y Especificidad , Úlcera
9.
Public Health Nutr ; : 1-11, 2021 Nov 08.
Artículo en Inglés | MEDLINE | ID: mdl-34743775

RESUMEN

OBJECTIVE: Food literacy is the knowledge, skills and behaviours needed to meet food needs and determine intake and is conceptualised as eleven components under four domains of planning and managing, selecting, preparing, and eating. Previous measures of food literacy vary in their adherence to the conceptualisation and ability to capture totality of eating. This study aimed to determine items for inclusion and exclusion in a food literacy item pool and capture the general public's interpretation of everyday food literacy practices. DESIGN: Beginning with an item pool from previous studies, cognitive interviews were conducted using think-aloud and verbal probing methods. Data were first analysed for applicability, clarity, ambiguity and logic, then for emergent themes to ensure items captured the totality of the participant's eating. SETTING: Australia. PARTICIPANTS: Australian residents over 18 years of age recruited via Facebook residential groups (n 20). RESULTS: Of the original 116 items, 11 items had limited applicability; 13 items had unclear references; 32 items had lexical problems and 11 items had logical problems. In total, 29 items were deleted, 31 retained and 56 revised. Thematic analysis revealed participants limited their responses to consider only conventional practices such as grocery shopping, cooking and planned meals rather than the totality of their eating. An additional eighty-four items were developed to address eating out, incidental eating occasions and inconsistencies between participants assumed correct knowledge and that of public health guidelines. This resulted in a refined 171-item pool. CONCLUSIONS: This study progresses the development towards a comprehensive, validated food literacy questionnaire.

10.
Public Health Nutr ; 24(7): 1608-1618, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-32383425

RESUMEN

OBJECTIVE: This study aimed to validate a nutrition knowledge questionnaire appropriate for use in Australia. DESIGN: Nutrition knowledge is essential in establishing and maintaining strategies that reduce the burden of disease and promote wellbeing. The General Nutrition Knowledge Questionnaire (GNKQ) was developed in the United Kingdom in 1999 and validated for Australia in 2008. Changes in national nutrition recommendations and food availability prompted the redevelopment and revalidation of the UK questionnaire in 2016. However, the Australian questionnaire had not been subsequently updated. SETTING: Australia. PARTICIPANTS: Content validity was determined using a sample of academic dietitians in Australia (n 8). Face validity was undertaken with retail employees (n 11) whose highest level of education was secondary school. Ninety-three undergraduate nutrition and engineering students at Queensland University of Technology completed the questionnaire for construct validity, and nineteen students were contacted a week later for test-retest reliability. RESULTS: In the 117-scored questionnaire, nutrition students scored consistently higher in each of the four sections and overall (87 %, M 102, IQR 95, 107) compared with engineering students (77 %, M 82, IQR 76, 87·25, P < 0·01). Internal reliability of the questionnaire was high (α = 0·92) as was test-retest reliability (rs = 0·96, ICC2,1 = 0·99). AUS-R NKQ determined significant differences between individuals with known higher levels of nutrition knowledge and obtained high validity, reliability and consistency within an Australian sample. CONCLUSIONS: AUS-R NKQ refined through this research is valid and would be an appropriate questionnaire for assessing the effectiveness of nutrition knowledge-based interventions for public health programmes, clinicians and researchers.


Asunto(s)
Conocimientos, Actitudes y Práctica en Salud , Estudiantes , Australia , Humanos , Reproducibilidad de los Resultados , Encuestas y Cuestionarios
11.
Altern Lab Anim ; 49(5): 197-208, 2021 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-34836462

RESUMEN

Across multiple sectors, including food, cosmetics and pharmaceutical industries, there is a need to predict the potential effects of xenobiotics. These effects are determined by the intrinsic ability of the substance, or its derivatives, to interact with the biological system, and its concentration-time profile at the target site. Physiologically-based kinetic (PBK) models can predict organ-level concentration-time profiles, however, the models are time and resource intensive to generate de novo. Read-across is an approach used to reduce or replace animal testing, wherein information from a data-rich chemical is used to make predictions for a data-poor chemical. The recent increase in published PBK models presents the opportunity to use a read-across approach for PBK modelling, that is, to use PBK model information from one chemical to inform the development or evaluation of a PBK model for a similar chemical. Essential to this process, is identifying the chemicals for which a PBK model already exists. Herein, the results of a systematic review of existing PBK models, compliant with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) format, are presented. Model information, including species, sex, life-stage, route of administration, software platform used and the availability of model equations, was captured for 7541 PBK models. Chemical information (identifiers and physico-chemical properties) has also been recorded for 1150 unique chemicals associated with these models. This PBK model data set has been made readily accessible, as a Microsoft Excel® spreadsheet, providing a valuable resource for those developing, using or evaluating PBK models in industry, academia and the regulatory sectors.


Asunto(s)
Modelos Biológicos , Programas Informáticos , Animales , Cinética , Medición de Riesgo
12.
J Interprof Care ; 35(6): 963-966, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33784925

RESUMEN

Interprofessional education (IPE), as preparation for interprofessional practice, is considered essential for quality, coordinated, outcome-focussed patient care. To develop capacity in our future healthcare practitioners, IPE needs to be developed within curricula, and opportunities provided to practise within the placement setting. The aim of this study was to examine the effect of a structured IPE placement program on students' perceptions of IPE within an authentic healthcare setting. This paper reports on changes in students' attitudes toward IPE, as measured by the SPICE-R2 instrument, in response to program involvement. Thirty-six students from six health professions participated in the study and reported significantly improved perceptions toward IPE, particularly in their understanding of roles and responsibilities, teamwork, and patient outcomes. The outcomes reinforce the importance of offering intentional and structured IPE activities during placement and the value provided to health students, preparing them for future collaborative practice.


Asunto(s)
Educación Interprofesional , Relaciones Interprofesionales , Actitud del Personal de Salud , Empleos en Salud , Personal de Salud/educación , Humanos
13.
Risk Anal ; 40(5): 1079-1091, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31971285

RESUMEN

Evacuation is frequently used by emergency managers and other officials as part of an overall approach to reducing the morbidity and mortality associated with hurricane landfall. In this study, the evacuation shelter capacity of the Houston-Galveston Metropolitan Statistical Area (MSA) was spatially assessed and shelter deficits in the region were estimated. These data provide essential information needed to eliminate shelter deficits and ensure a successful evacuation from a future storm. Spatial statistical methods-Global Moran's I, Anselin Local Moran's I (Local Indicators of Spatial Association [LISA]), and Hot Spot Analysis (Getis-Ord Gi*) were used to assess for regional spatial autocorrelation and clustering of evacuation shelters in the Houston-Galveston MSA. Shelter deficits were estimated in four ways-the aggregate deficit for the Houston-Galveston MSA, by evacuation Zip-Zone, by county, and by distance or radii of evacuation Zip-Zone. Evacuation shelters were disproportionately distributed in the region, with lower capacity shelters clustered closer to evacuation Zip-Zones (50 miles from the Coastal Zip-Zone), and higher capacity shelters clustered farther away from the zones (120 miles from the Coastal Zip-Zone). The aggregate shelter deficit for the Houston-Galveston MSA was 353,713 persons. To reduce morbidity and mortality associated with future hurricanes in the Houston-Galveston MSA, authorities should consider the development and implementation of policies that would improve the evacuation shelter capacity of the region. Eliminating shelter deficits, which has been done successfully in the state of Florida, is an essential element of protecting the public from hurricane impacts.

15.
J Hist Med Allied Sci ; 74(4): 416-439, 2019 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-31553441

RESUMEN

This essay explores the uses of phrenological theory in the realm of jurisprudence between the mid-1830s and 1850s, focusing in particular on the adoption and circulation of phrenological language within medico-legal circles through this period. The article begins by contextualizing medical jurisprudence in early America; at the same time that phrenology was gaining ground in the United States, theories of medical jurisprudence were in flux. I next turn to the concept of the propensities in phrenological theory and their relationship to theories of moral insanity developed in the same period. This article concludes with an exploration of explicit and implicit uses of phrenology, focusing on court cases featuring phrenological expertise or language. The article thus suggests both the uses of phrenology for the building of medico-legal expertise and the extent to which phrenological language around the propensities inflected lay and medico-legal discourse around criminal responsibility and insanity.


Asunto(s)
Defensa por Insania/historia , Jurisprudencia/historia , Frenología/historia , Historia del Siglo XIX , Humanos , Defensa por Insania/estadística & datos numéricos , Estados Unidos
16.
BMC Health Serv Res ; 17(1): 341, 2017 05 09.
Artículo en Inglés | MEDLINE | ID: mdl-28486980

RESUMEN

BACKGROUND: HIV treatment models in Africa are labour intensive and require a high number of skilled staff. In this context, task-shifting is considered a feasible alternative for ART service delivery. In 2006, a lay health cadre of expert patients (EPs) at a tertiary referral HIV clinic in Zomba, Malawi was capacitated. There are few evaluations of EP program efficacy in this setting. Triage is the process of prioritizing patients in terms of the severity of their condition and ensures that no harmful delays occur to treatment and care. This study evaluates the safety of task-shifting triage, in an ambulatory low resource setting, to EPs. METHODS: As a quality improvement exercise in April 2010, formal triage training was conducted by adapting the World Health Organization Emergency Triage Assessment and Treatment Triage Module Guidelines. A cross sectional observation study was conducted 2 years after the intervention. Triage assessments performed by EPs were repeated by a clinical officer (gold standard) to assess sensitivities, specificities, positive and negative predictive values for EP triage scores. Proportions were calculated for categories of disposition by stratifying by EP and clinician triage scores. RESULTS: A total of 467 patients were triaged by 7 EPs and re-triaged by clinical officers. With combined triage scores for emergency and priority patients we report a sensitivity of 85% and specificity of 74% for the EP scoring, with a low positive predictive value (41%) and a high negative predictive value (96%). We calculate a serious miss rate of EP scoring (i.e. missed priority or emergency patients) as 2.2%. Admission rates to hospital were highest among those patients triaged as emergency cases either by the EP's (21%) or the clinicians (83%). Fewer patients triaged as priority by either EPs (5%) or clinicians (15%) were admitted to hospital, however these patients had the highest prevalence of same day lab testing and/or specialty referral. CONCLUSIONS: Our study provides reassurance that in the context of adequate training and ongoing supervision, task-shifting triage to lay health care workers does not necessarily lead to less accurate triaging. EPs have a tendency to be more conservative in over-triaging patients.


Asunto(s)
Infecciones por VIH , Personal de Salud , Mejoramiento de la Calidad , Triaje , Adulto , Instituciones de Atención Ambulatoria/organización & administración , Estudios Transversales , Femenino , Infecciones por VIH/tratamiento farmacológico , Personal de Salud/educación , Hospitalización/estadística & datos numéricos , Humanos , Malaui , Masculino , Persona de Mediana Edad , Evaluación de Programas y Proyectos de Salud , Triaje/métodos , Triaje/normas
17.
Inorganica Chim Acta ; 466: 442-450, 2017 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-29217867

RESUMEN

We report the synthesis and characterization of new ruthenium(II) and iridium(III) complexes of a new bidentate chelate, NHCR'-pyOR (OR = OMe, OtBu, OH and R' = Me, Et). Synthesis and characterization studies were done on the following compounds: four ligand precursors (1-4); two silver complexes of these NHCR'-pyOR ligands (5-7); six ruthenium complexes of the type [η6-(p-cymene)Ru(NHCR'-pyOR)Cl]X with R' = Me, Et and R = Me, tBu, H and X = OTf-, PF6- and PO2F2- (8-13); and two iridium complexes, [Cp*Ir(NHCMe-pyOtBu)Cl]PF6 (14) and [Cp*Ir(NHCMe-pyOH)Cl]PO2F2 (15). The complexes are air stable and were isolated in moderate yield. However, for the PF6- salts, hydrolysis of the PF6- counter anion to PO2F2- during t-butyl ether deprotection was observed. Most of the complexes were characterized by 1H and 13C-NMR, MS, IR, and X-ray diffraction. The ruthenium complexes [η6-(p-cymene)Ru(NHCMe-pyOR)Cl]OTf (R = Me (8) and tBu (9)) were tested for their ability to accelerate CO2 hydrogenation and formic acid dehydrogenation. However, our studies show that the complexes transform during the reaction and these complexes are best thought of as pre-catalysts.

18.
Am J Physiol Endocrinol Metab ; 308(7): E592-602, 2015 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-25648831

RESUMEN

Human islet research is providing new insights into human islet biology and diabetes, using islets isolated at multiple US centers from donors with varying characteristics. This creates challenges for understanding, interpreting, and integrating research findings from the many laboratories that use these islets. In what is, to our knowledge, the first standardized assessment of human islet preparations from multiple isolation centers, we measured insulin secretion from 202 preparations isolated at 15 centers over 11 years and noted five distinct patterns of insulin secretion. Approximately three quarters were appropriately responsive to stimuli, but one quarter were dysfunctional, with unstable basal insulin secretion and/or an impairment in stimulated insulin secretion. Importantly, the patterns of insulin secretion by responsive human islet preparations (stable Baseline and Fold stimulation of insulin secretion) isolated at different centers were similar and improved slightly over the years studied. When all preparations studied were considered, basal and stimulated insulin secretion did not correlate with isolation center, biological differences of the islet donor, or differences in isolation, such as Cold Ischemia Time. Dysfunctional islet preparations could not be predicted from the information provided by the isolation center and had altered expression of genes encoding components of the glucose-sensing pathway, but not of insulin production or cell death. These results indicate that insulin secretion by most preparations from multiple centers is similar but that in vitro responsiveness of human islets cannot be predicted, necessitating preexperimental human islet assessment. These results should be considered when one is designing, interpreting, and integrating experiments using human islets.


Asunto(s)
Insulina/metabolismo , Islotes Pancreáticos/metabolismo , Investigación , Donantes de Tejidos , Obtención de Tejidos y Órganos , Adolescente , Adulto , Anciano , Niño , Femenino , Humanos , Secreción de Insulina , Masculino , Persona de Mediana Edad , Manejo de Especímenes , Donantes de Tejidos/estadística & datos numéricos , Donantes de Tejidos/provisión & distribución , Obtención de Tejidos y Órganos/estadística & datos numéricos , Adulto Joven
19.
Theor Appl Genet ; 127(7): 1583-91, 2014 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24821525

RESUMEN

KEY MESSAGE: A point mutation in the AHAS1 gene leading to resistance to imidazolinone in chickpea was identified. The resistance is inherited as a single gene. A KASP marker targeting the mutation was developed. Weed control in chickpea (Cicer arietinum L.) is challenging due to poor crop competition ability and limited herbicide options. A chickpea genotype with resistance to imidazolinone (IMI) herbicides has been identified, but the genetic inheritance and the mechanism were unknown. In many plant species, resistance to IMI is caused by point mutation(s) in the acetohydroxyacid synthase (AHAS) gene resulting in an amino acid substitution preventing herbicide attachment to the molecule. The main objective of this research was to characterize the resistance to IMI herbicides in chickpea. Two homologous AHAS genes namely AHAS1 and AHAS2 sharing 80 % amino acid sequence similarity were identified in the chickpea genome. Cluster analysis indicated independent grouping of AHAS1 and AHAS2 across legume species. A point mutation in the AHAS1 gene at C675 to T675 resulting in an amino acid substitution from Ala205 to Val205 confers the resistance to IMI in chickpea. A KASP marker targeting the point mutation was developed and effectively predicted the response to IMI herbicides in a recombinant inbred (RI) population of chickpea. The RI population was used in molecular mapping where the major locus for the reaction to IMI herbicide was mapped to chromosome 5. Segregation analysis across an F2 population and RI population demonstrated that the resistance is inherited as a single gene in a semi-dominant fashion. The simple genetic inheritance and the availability of KASP marker generated in this study would speed up development of chickpea varieties with resistance to IMI herbicides.


Asunto(s)
Cicer/genética , Genes de Plantas , Herbicidas/farmacología , Proteínas de Plantas/genética , Acetolactato Sintasa/genética , Secuencia de Aminoácidos , Cicer/clasificación , Análisis por Conglomerados , ADN de Plantas/genética , Resistencia a la Enfermedad , Genotipo , Datos de Secuencia Molecular , Mutación , Proteínas de Plantas/metabolismo , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN
20.
J Nutr Educ Behav ; 2024 Aug 20.
Artículo en Inglés | MEDLINE | ID: mdl-39162676

RESUMEN

OBJECTIVE: This study aimed to explore the preprofessional identity of undergraduate nutrition and dietetic students to guide curriculum development to better support the expectations of students and promote career readiness in a changing profession. DESIGN: Qualitative focus group discussions in March, 2021. SETTING: An Australian university. PARTICIPANTS: First-year students enrolled in the Bachelor of Nutrition (n = 50) or Bachelor of Dietetics (n = 58) at the University of the Sunshine Coast. MAIN OUTCOME MEASURES: Student sociodemographics, motivations for and influences on career choice and preprofessional identity, expectations of professional competency and practice, degree, and career expectations. ANALYSIS: Descriptive statistics were conducted, and focus group discussions were analyzed using the Framework Approach. RESULTS: Motivations and skills were consistent across both cohorts, centering on an interest in nutrition and respectful, professional conduct and communication. Expectations were similar across both degrees, with a focus on placement, real-world learning experiences, and staff support. Career expectations for both cohorts included business ownership. CONCLUSIONS AND IMPLICATIONS: This research provided an understanding of students' preprofessional identity, which was similar for both nutrition and dietetics students. Motivations identified in this research can be used to inform activities across nutrition and dietetic programs that support career readiness.

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