Mapping of cytosol-facing organelle outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells.

The cytosol-facing outer membrane (OM) of organelles communicates with other cellular compartments to exchange proteins, metabolites, and signaling molecules. Cellular surveillance systems also target OM-resident proteins to control organellar homeostasis and ensure cell survival under stress. However, the OM proximity proteomes have never been mapped in plant cells since using traditional approaches to discover OM proteins and identify their dynamically interacting partners remains challenging. In this study, we developed an OM proximity labeling (OMPL) system using biotin ligase-mediated proximity biotinylation to identify the proximity proteins of the OMs of mitochondria, chloroplasts, and peroxisomes in living Arabidopsis (Arabidopsis thaliana) cells. Using this approach, we mapped the OM proximity proteome of these three organelles under normal conditions and examined the effects of the ultraviolet-B (UV-B) or high light (HL) stress on the abundances of OM proximity proteins. We demonstrate the power of this system with the discovery of cytosolic factors and OM receptor candidates potentially involved in local protein translation and translocation. The candidate proteins that are involved in mitochondrion-peroxisome, mitochondrion-chloroplast, or peroxisome-chloroplast contacts, and in the organellar quality control system are also proposed based on OMPL analysis. OMPL-generated OM proximity proteomes are valuable sources of candidates for functional validation and suggest directions for further investigation of important questions in cell biology.

Creating perfect composite vortex beams with a single all-dielectric geometric metasurface.

Optical vortex beam carrying orbit angular momentum has been extensively researched and applied recently. Among which a perfect vortex beam (PVB) has attracted much attention owing to its topological charge (TC)-irrelevant intensity profile. However, the morphology singularity, as well as implementation complexity of the PVB tie the degree of freedom for multiplexing. Herein, by introducing the concept of a composite vortex beam, we originally propose a novel kind of PVB - perfect composite vortex beam (PCVB) - which possesses a rosette-like intensity pattern that is exactly correlated with the TC and can be directly generated using a single all-dielectric geometric metasurface rather than bulky optical systems. We numerically simulate the broadband generation of the proposed PCVB with various TCs, sizes, and rotation angles. To further explore the potential of our design in practical applications, we demonstrated the coaxial array of the PCVBs and detected their optical angular force for manipulating nanoparticles. We believe that our fruitage may pave a desirable avenue for optical communication, information processing, and optical manipulation.