RESUMEN
Evaluation of growth hormone dependent IGFBP3, stable in time and with no circadian variations is a useful parameter for diagnosing growth hormone deficit. The IGFBP3 was evaluated using sera, collected and kept at -20 degrees C, from subjects classified according to auxologic characters and response sup. or inf. to 8 ng/ml GH after two stimulation tests and median nocturnal GH sup. or inf. to 3 ng/ml. Two groups were studied: 1) Small stature GHD (growth hormone deficiency): 14 cases; 2) Constitutional small stature (RCC,BSF): 12 cases. A third group composed of 8 normal height, weight and disease-free children formed the control group. The IGFBP3 values were below the 5th percentile in 86% of GHD cases, between the 5th and 95th percentile in 66.6% of constitutionally short stature children and in all normal controls was about the 50th percentile. The IGFBP3 also shows a statistically significant correlation between median nocturnal GH, both in deficient and constitutionally short stature groups (p < 0.01).
Asunto(s)
Proteínas Portadoras/sangre , Trastornos del Crecimiento/diagnóstico , Hormona del Crecimiento/deficiencia , Somatomedinas/análisis , Adolescente , Niño , Preescolar , Femenino , Trastornos del Crecimiento/sangre , Trastornos del Crecimiento/etiología , Humanos , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina , Masculino , Valores de Referencia , Análisis de RegresiónRESUMEN
Serum IGFBP3 levels were tested in 7 GHD children before the rGH therapy, at the end of the same therapy and 1, 2, 4, and 8 weeks later. The IGFBP3 concentration returned to the pre-therapy levels only 6 weeks after the end of the therapy.
Asunto(s)
Proteínas Portadoras/sangre , Trastornos del Crecimiento/tratamiento farmacológico , Hormona del Crecimiento/administración & dosificación , Inhibidores de Crecimiento/sangre , Somatomedinas/análisis , Niño , Preescolar , Femenino , Estudios de Seguimiento , Hormona del Crecimiento/deficiencia , Humanos , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina , Masculino , Radioinmunoensayo , Proteínas Recombinantes/administración & dosificación , Factores de TiempoRESUMEN
Growth Hormone (GH) and serum Bone GLA-Protein (BGP or Osteocalcin), a sensitive and specific marker of bone turnover, were measured in 5 children with growth retardation, during 12 h period from 8 p.m. every 30'. A nocturnal periodicity in Osteocalcin was found: BGP rose slightly during sleep in the patients studied, maximum concentration being reached between 3 and 5 a.m. There were no consistent correlations between Osteocalcin concentration and circulating levels of GH in the subjects tested. BGP determination may be of interest in the evaluation of children with short stature but standardized analytical conditions remain to be determined.