RESUMEN
Extraction of Furcellaria lumbricalis by potassium hydroxide solution shows that the maximum gel strength of polysaccharides separated is attained by extraction at concentration 0.16 M KOH. Crude extract from this algal species has a complicated monosaccharide composition; a drastic increase of gel strength by alkaline extraction of the polysaccharide mixture cannot be explained through the composition of a major (kappa) fraction described earlier. Alkaline treatment is also suitable for accelerating the extraction of polysaccharides from Coccotylus truncatus overlaid with thick epithelium. Viscous galactans from C. truncatus are more stable to alkali, their composition is simpler and close to iota carrageenan. The formation of mixed kappa and iota carrageenan gels as a result of simultaneous extraction of both seaweeds causes levelling of theological properties of different extraction fractions.
Asunto(s)
Eucariontes/química , Galactanos/química , Estonia , Geles , Espectroscopía de Resonancia Magnética , Microscopía Electrónica de Rastreo , Monosacáridos/análisis , Océanos y Mares , Relación Estructura-ActividadRESUMEN
The effect of the number of active groups of new affinity supports--odigose and odifil (ethylsulfo-activated agarose) on the retention of the specific activity of muscle aldolase was investigated. The active center of the enzyme includes lysine able to react with activated supports. The aldolase completely retained the specific activity after immobilization on the abovementioned relatively high-substituted supports, on which other enzymes, e.g. phosphorylase B, NAD-kinase from pigeon heart, were partially or completely inactivated. The aldolase was inactivated when being immobilized on more substituted supports. The enzyme specific activity completely retained if the high substituted supports were preliminary incubated at 37 degrees to destroy some diazo-groups.