Evaluation of a Novel Immunochromatographic Assay Using Monoclonal Antibodies against the Matrix Protein of Human Metapneumovirus.

BACKGROUND: The aim of this study was to determine the sensitivity and specificity of a novel immunochromatographic (IC) assay (APD1806) using monoclonal antibodies against the matrix (M) protein of human metapneumovirus (hMPV) for detection of hMPV from nasopharyngeal swab samples based on the results of real-time RT-PCR. METHODS: Nasopharyngeal swab samples taken from 189 patients aged 0 - 5 years who were suspected of having respiratory tract infections associated with hMPV were used in this study. The samples were tested both by the IC assay and by real-time RT-PCR for detection of hMPV. RESULTS: The sensitivity and specificity of the IC assay for detection of hMPV were 88.8% (95/107) and 92.7% (76/82), respectively. CONCLUSIONS: The IC assay using monoclonal antibodies against the M protein of hMPV is an accurate and fast assay that is suitable as a diagnostic tool for hMPV infection. The optimal timing of the IC assay is 12 hours or more after the onset of fever due to hMPV infection.

Clinical effectiveness of four neuraminidase inhibitors (oseltamivir, zanamivir, laninamivir, and peramivir) for children with influenza A and B in the 2014-2015 to 2016-2017 influenza seasons in Japan.

The clinical effectiveness of four neuraminidase inhibitors (NAIs) (oseltamivir, zanamivir, laninamivir, and peramivir) for children aged 0 months to 18 years with influenza A and B were investigated in the 2014-2015 to 2016-2017 influenza seasons in Japan. A total of 1207 patients (747 with influenza A and 460 with influenza B) were enrolled. The Cox proportional-hazards model using all of the patients showed that the duration of fever after administration of the first dose of the NAI was shorter in older patients (hazard ratio = 1.06 per 1 year of age, p < 0.001) and that the duration of fever after administration of the first dose of the NAI was shorter in patients with influenza A infection than in patients with influenza B infection (hazard ratio = 2.21, p < 0.001). A logistic regression model showed that the number of biphasic fever episodes was 2.99-times greater for influenza B-infected patients than for influenza A-infected patients (p < 0.001). The number of biphasic fever episodes in influenza A- or B-infected patients aged 0-4 years was 2.89-times greater than that in patients aged 10-18 years (p = 0.010), and the number of episodes in influenza A- or B-infected patients aged 5-9 years was 2.13-times greater than that in patients aged 10-18 years (p = 0.012).

Caregiver treatment satisfaction is improved together with children's asthma control: Prospective study for budesonide monotherapy in school-aged children with uncontrolled asthma symptoms.

BACKGROUND: If asthmatic children cannot obtain sufficient control of their disease, not only do they suffer from asthma symptoms, but the daily life activities of their caregivers are also disrupted. We investigated the effectiveness of an inhaled corticosteroid (ICS) for symptom control in previously ICS-untreated school-aged asthmatic children as well as caregiver treatment satisfaction (CTS). METHODS: A multicenter, open-label, single-arm study on 12-week ICS (budesonide Turbuhaler®) monotherapy was undertaken in subjects aged 5-15 years with bronchial asthma not treated with ICS during the previous 3 months. At 0, 4, 8, and 12 weeks after start of ICS administration, Japanese Pediatric Asthma Control Program (JPAC) scores, and CTS scores were summated and lung function measured. At weeks 0 and 12, questionnaires on caregiver anxiety were also assessed. RESULTS: Seventy-five patients were enrolled, and 69 assessed. Ninety percent of subjects had been treated with asthma controller medication except ICS before study enrollment. JPAC score and CTS score were improved significantly at weeks 4, 8, and 12 (p < 0.001). With regard to CTS, more than half of caregivers showed a perfect score at weeks 8 and 12. There was a significant correlation between JPAC score and CTS score. Lung function and caregiver anxiety were also improved, and good compliance with treatment was observed during the intervention. CONCLUSIONS: If treating ICS-untreated school-aged asthmatic children with uncontrolled symptoms, ICS monotherapy can improve CTS along with improving asthma control.

P1 gene of Mycoplasma pneumoniae isolated from 2016 to 2019 and relationship between genotyping and macrolide resistance in Hokkaido, Japan.

We characterized 515 Mycoplasma pneumoniae specimens in Hokkaido. In 2013 and 2014, the p1 gene type 1 strain, mostly macrolide-resistant, was dominant and the prevalence of macrolide resistance was over 50 %. After 2017, the p1 gene type 2 lineage, mostly macrolide-sensitive, increased and the prevalence of macrolide resistance became 31.0 % in 2017, 5.3 % in 2018 and 16.3 % in 2019.

Drying dissipative patterns of the colloidal crystals of silica spheres in an dc-electric field.

Drying dissipative structural patterns of the colloidal crystals of silica spheres were studied under an dc-electric field. Platinum plate electrodes of anode and cathode were set on a cover glass. The broad hills accumulated with the spheres were observed at the outer edges of the dried film without and also with the electric fields. The column-like structures were formed by the electric flux, and movement of the spheres took place toward anode. The dried film kept colloidal crystal structure, where the nearest-neighbored spheres contact each other more compactly in the areas closer to the anode. Drying times needed for the complete dryness of the suspensions decreased as the strength of the electric field increased. Addition of sodium chloride to the suspensions retarded the movement of spheres toward the anode substantially.

Rheological properties of deionized Chinese ink.

Rheological properties for Chinese ink in exhaustively deionized aqueous media were carefully examined. In the steady shear measurement, the shear viscosities of the ink could be well explained by considering the "effective" volume fraction of the particles in the ink including the electrical double layers and by using Einstein's equation for dilute suspension viscosity, when the particle volume fraction was substantially low. In the case that the volume fraction was higher, the shear viscosities showed extremely higher than those from Einstein's prediction, though the ink remained a Newtonian liquid. In the stress-strain measurement, the shear moduli were observed at strain smaller than 0.2. The "weak" aggregation among the particles in the ink under no shear or low shear rates was supported. It should be noted that the glue in the suspension plays an important role for the good liquidity of the ink and for the "weak" bridges among the particles resulting its good dispersion stability.

Colloidal crystals of core-shell type spheres with poly(styrene) core and poly(ethylene oxide) shell.

Elastic modulus and crystal growth kinetics have been studied for colloidal crystals of core-shell type colloidal spheres (diameter=160-200 nm) in aqueous suspension. Crystallization properties of three kinds of spheres, which have poly(styrene) core and poly(ethylene oxide) shell with different oxyethylene chain length (n=50, 80 and 150), were examined by reflection spectroscopy. The suspensions were deionized exhaustively for more than 1 year using mixed bed of ion-exchange resins. The rigidities of the crystals range from 0.11 to 120 Pa and from 0.56 to 76 Pa for the spheres of n=50 and 80, respectively, and increase sharply as the sphere volume fraction increase. The g factor, parameter for crystal stability, range from 0.029 to 0.13 and from 0.040 to 0.11 for the spheres of n=50 and 80, respectively. These g values indicate the formation of stable crystals, and the values were decreased as the sphere volume fraction increased. Two components of crystal growth rate coefficients, fast and slow, were observed in the order from 10(-3) to 10(1)s(-1). This is due to the secondary process in the colloidal crystallization mechanism, corresponding to reorientation from metastable crystals formed in the primary process and/or Ostwald-ripening process. There are no distinct differences in the structural, kinetic and elastic properties among the colloidal crystals of the different core-shell size spheres, nor difference between those of core-shell spheres and silica or poly(styrene) spheres. The results are very reasonably interpreted by the fact that colloidal crystals are formed in a closed container owing to long-range repulsive forces and the Brownian movement of colloidal spheres surrounded by extended electrical double layers, and their formation is not influenced by the rigidity and internal structure of the spheres.

Dish-like drying patterns of the water-soluble gelatin sheet wetted by an aqueous droplet.

Drying dissipative patterns of the water-soluble gelatin sheet wetted by an aqueous droplet were observed as a function of time elapsed. The arrayed clusters of dishes formed at the broad ring area and grew outward toward multiple arrays with time. The drying patterns formed by the cooperative contribution of wetting, swelling, dissolving, evaporative, convectional, sedimentary and solidifying processes. Drying patterns were studied also for aqueous ethanol, aqueous NaCl solutions and dispersions of colloidal silica and poly(methyl methacrylate) spheres. The dish patterns were observed for the water-soluble substrates, for the first time, in this work.

Microgravity effects on thermodynamic and kinetic properties of colloidal dispersions.

Microgravity experiments on the physicochemical properties of colloidal dispersions reported hitherto have been reviewed. In microgravity, reliability and reproducibility of experimental data have been improved significantly by the elimination of convection in the suspension. Vanishment of the excess downward diffusion in microgravity has also produced a significant effect on properties of colloidal suspensions. For example, colloidal crystallization rates decreased in microgravity, whereas colloidal alloy crystallization rates increased. These results demonstrate the important role of the segregation effect in normal gravity. Colloidal silica formation reactions are retarded in microgravity, a phenomenon that is correlated deeply with disappearance of the downward diffusion of heavy products.

Evaluation of prognostic factors and clinical outcome in elderly patients in whom expansive laminoplasty is performed for cervical myelopathy due to multisegmental spondylotic canal stenosis. A retrospective comparison with younger patients.

OBJECT: It remains unclear whether elderly patients with compressive cervical myelopathy can be expected to experience a promising surgery-related outcome after undergoing expansive laminoplasty. The purposes of this study were to evaluate the efficacy of expansive laminoplasty in elderly patients with cervical myelopathy due to multisegmental spondylotic canal stenosis and to analyze the effect of preoperative prognostic factors on outcome in elderly compared with younger patients. METHODS: The authors reviewed the cases of 22 elderly (> 70 years of age) and 39 younger patients in whom expansive open-door laminoplasty was performed for cervical myelopathy due to multisegmental spondylotic canal stenosis. The pre- and 12-month postoperative clinical symptoms were evaluated using the Japanese Orthopaedic Association (JOA) disability scale. Factors affecting the clinical outcome were statistically analyzed by evaluating the recovery rate calculated from the JOA scale. There were no significant differences in the mean value of the preoperative factors, especially preoperative duration of symptoms and severity of preoperative disease, between the elderly and younger patient groups. In all patients, age at the time of the operation was shown to exert no significant influence on clinical outcome. The mean recovery rate was 58.8% in the elderly group and 61.8% in the younger group, and there was no significant intergroup difference. Improvement or attenuation in impaired upper- and lower-leg motor function was shown in all patients as was an absence in decline of sensory impairment of the extremities. In the elderly group, both the duration of symptoms and the severity of canal stenosis significantly (p < 0.05) affected the clinical outcome. In the younger group, the severity of preoperative symptoms had a significant (p < 0.05) influence on clinical outcome, whereas duration of the symptoms did not appreciably affect clinical improvement. CONCLUSIONS: Open-door expansive laminoplasty showed a promising effect on clinical outcome in elderly and younger patients with multisegmental cervical canal stenosis. Significant predictive factors for clinical outcome in the elderly patients were the duration of symptoms and the severity of stenosis, which may involve the static factor causing the cervical myelopathy. To improve the elderly patients' disability, surgery must be performed as early as possible before irreversible changes in the spinal cord develop.

Dissipative crystallization of potassium salt of poly(riboadenylic acid).

Dissipative patterns during the course of dryness of aqueous solution of potassium salt of poly(riboadenylic acid) (KPolyA) in the presence of potassium chloride were studied on a cover glass, a watch glass and a glass dish. Accumulation of KPolyA polymers forming the broad ring area and the drastic change in size and shape of the polymer single crystals depending on the location of the dried film, which are the typical effects of the dissipative crystallization, took place. Polymer crystals formed were spherulites, dendritic and rod-like assemblies, which are composed of the single or double helical chains depending on the pH-value of the initial solution.

Electro-optic effects of colloidal crystals.

Many kinds of electro-optic effects of colloidal crystals are observed and discussed on the basis of the fundamental properties of colloidal crystals themselves. Several electro-optic effects of colloidal crystals have been found by the authors mainly by use of light-scattering, reflection- and transmitted-light intensity measurements in an electric field, (a) waveform deformation, (b) phase-shift effects, (c) second-order harmonics generation, (d) self-resonance frequency generation (characteristic frequency and harmonic oscillation), (e) peak wavelength-shift effects and (f) waveform transformation. These electro-optic responses are explained successfully by the resonance-, visco-elastic- and structural relaxation-parameters of colloidal crystals.

Drying dissipative structures of lycopodium spore particles in aqueous dispersion.

Drying dissipative structures of aqueous dispersions of lycopodium particles (31 microm in average diameter) from the spores of Lycopodium clavatum were studied as a function of the particle concentrations in the presence and the absence of sodium chloride. The drying patterns formed on a cover glass, a watch glass and a Petri glass dish were observed macroscopically and microscopically. Lycopodium particles were the combination of hemisphere and tetrahedron in their shape and possessed the weakly acidic groups on their surfaces. The vague broad ring structure was observed even for the very large colloidal particles, and their size on a cover glass decreased as particle concentration decreased. Microscopic drying patterns almost disappeared except the short chain-like patterns. These observations support that the convectional contribution on the dissipative drying pattern formation is still effective in the lycopodium suspensions, though the convectional forces in the suspension are very weak compared with the sedimentation forces.

Drying dissipative structures of nonionic surfactants in aqueous solution.

Macroscopic and microscopic dissipative structural patterns form in the course of drying a series of aqueous solutions of polyoxyethylenealkyl ethers. The shift from the single round hill with accumulated surfactant molecules to the broad ring patterns of the hill in a macroscopic scale occurs as the HLB (hydrophile-liophile balance) of the surfactant molecules increases. The patterns correlate intimately with the HLB values of the surfactants. Microscopic patterns of small blocks, starlike patterns, and branched strings are formed. The size and shape of the surfactant molecules themselves influence the drying patterns in part. The pattern area and the time to dryness have been discussed as a function of surfactant concentration and HLB of the surfactants. The convection flow of water accompanying the surfactant molecules, the change in the contact angles at the drying frontier between solution and substrate in the course of dryness, and interactions among the surfactants and substrate are important for the macroscopic pattern formation. Microscopic patterns are determined in part by the shape and size of the molecules, translational Brownian movement of the surfactant molecules, and the electrostatic and hydrophobic interactions between surfactants and/or between the surfactant and substrate in the course of solidification.

Irsogladine malate up-regulates gap junctional intercellular communication between pancreatic cancer cells via PKA pathway.

INTRODUCTION: Gap junctions (GJs) are intercellular channels that aid communication between coupling cells and may play a critical role in cell differentiation and growth. Connexins (Cxs) are structural proteins of GJs. Though several reports have demonstrated that Cx expression decreases in various malignant tumors, a pancreatic cancer cell line, PANC-1, was reported to express Cx43 mRNA. It is known that irsogladine malate (IM) can up-regulate gap junctional intercellular communication (GJIC). We examined the effects of IM on GJ between pancreatic cancer cells (PC cells) and the mechanism of GJ up-regulation. METHODOLOGY: GJIC between PC cells (PANC-1) was evaluated by dye transfer methods. The expression of Cx43 was estimated by Western blot analysis with immunoprecipitation sample and immunohistochemical analysis. Intracellular cAMP level was estimated by enzyme-linked immunoassay. RESULTS: IM increased cell coupling in a dose-dependent manner (0M-10 ). Western blot analysis of Cx43 revealed that PANC-1 cells expressed Cx43 protein. Treatment with IM was found to move localization of Cx43 immunoreactive spots from the cytoplasm to boundary lesions with neighboring cells, but no major change was seen in the phosphorylation state of Cx43. Intracellular cAMP level was increased by IM. The PKA inhibitor H-89 and adenylyl cyclase inhibitor SQ22536 inhibited the effects of IM. CONCLUSION: These results suggest that IM up-regulates GJIC between PC cells via regulation of the PKA pathway. It also suggests a useful adjuvant of IM to pancreatic cancer therapy.

Screening of genes specifically activated in the pancreatic juice ductal cells from the patients with pancreatic ductal carcinoma.

Pancreatic ductal carcinoma (PDC) is one of the most intractable human malignancies. Surgical resection of PDC at curable stages is hampered by a lack of sensitive and reliable detection methods. Given that DNA microarray analysis allows the expression of thousands of genes to be monitored simultaneously, it offers a potentially suitable approach to the identification of molecular markers for the clinical diagnosis of PDC. However, a simple comparison between the transcriptomes of normal and cancerous pancreatic tissue is likely to yield misleading pseudopositive data that reflect mainly the different cellular compositions of the specimens. Indeed, a microarray comparison of normal and cancerous tissue identified the INSULIN gene as one of the genes whose expression was most specific to normal tissue. To eliminate such a "population-shift" effect, the pancreatic ductal epithelial cells were purified by MUC1-based affinity chromatography from pancreatic juice isolated from both healthy individuals and PDC patients. Analysis of these background-matched samples with DNA microarrays representing 3456 human genes resulted in the identification of candidate genes for PDC-specific markers, including those for AC133 and carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM7). Specific expression of these genes in the ductal cells of the patients with PDC was confirmed by quantitative real-time polymerase chain reaction analysis. Microarray analysis with purified pancreatic ductal cells has thus provided a basis for the development of a sensitive method for the detection of PDC that relies on pancreatic juice, which is routinely obtained in the clinical setting.