RESUMEN
Isolated perfused rat hearts exposed to increased calcium concentrations demonstrated a marked activation of phosphorylase, the maximum occurring about 15 seconds after the peak of the positive inotropic response. These observations provide an explanation for the failure of earlier investigations to demonstrate a significant calcium-induced increase in the active form of phosphorylase in the intact heart.
Asunto(s)
Calcio/farmacología , Miocardio/enzimología , Fosfotransferasas/metabolismo , Animales , Perros , Femenino , Perfusión , RatasRESUMEN
Administration of estradiol to ovariectomized rats induced new synthesis of uterine hexokinase which was prevented by actinomycin D, 5-fluorouracil, cycloheximide, or ethionine. The estradiol-induced increase in uterine hexokinase activity was detectable as early as 4 hours. The increase in enzyme activity was dependent upon the dose of the hormone. The evidence indicates that the increased hexokinase activity induced by 17beta-estradiol may entail an acceleration of the synthesis of certain RNA species.
Asunto(s)
Inducción Enzimática , Estradiol/farmacología , Hexoquinasa/biosíntesis , Útero/enzimología , Animales , Castración , Cicloheximida/farmacología , Dactinomicina/farmacología , Antagonistas de Estrógenos , Etionina/farmacología , Femenino , Fluorouracilo/farmacología , Cinética , Ovario/cirugía , Fosfofructoquinasa-1/metabolismo , ARN/biosíntesis , Ratas , Estimulación Química , Útero/efectos de los fármacosAsunto(s)
Corazón/efectos de los fármacos , Hormonas Hipofisarias/farmacología , Hormonas Tiroideas/farmacología , Animales , Fluoroacetatos/farmacología , Fluorometría , Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo , Glucólisis/efectos de los fármacos , Hormona del Crecimiento/farmacología , Hexoquinasa/metabolismo , Hipofisectomía , Hipotiroidismo/metabolismo , Miocardio/enzimología , Fosfofructoquinasa-1/metabolismo , Hipófisis/fisiología , Piruvato Quinasa/metabolismo , RatasAsunto(s)
Estrógenos/farmacología , Glucosa-6-Fosfato Isomerasa , Progesterona/farmacología , Testosterona/farmacología , Útero/enzimología , Animales , Castración , Dactinomicina/farmacología , Dietilestilbestrol/farmacología , Estradiol/farmacología , Estriol/farmacología , Antagonistas de Estrógenos , Estrógenos/administración & dosificación , Estrona/farmacología , Etionina/farmacología , Femenino , Ovario/fisiología , Ratas , Estimulación Química , Factores de TiempoAsunto(s)
Estradiol/farmacología , Glicerofosfatos/biosíntesis , Lactatos/biosíntesis , Útero/metabolismo , Animales , Castración , Cicloheximida/farmacología , ADN/análisis , Dactinomicina/farmacología , Etanol/farmacología , Femenino , Glucosa/análisis , Glucosa/metabolismo , Glicerofosfatos/análisis , Glucólisis/efectos de los fármacos , Lactatos/análisis , Tamaño de los Órganos , Ovario/fisiología , Fosfatos/metabolismo , Progesterona/farmacología , Pirrolidinas/farmacología , Ratas , Factores de Tiempo , Útero/análisis , Útero/efectos de los fármacos , Útero/enzimologíaAsunto(s)
DDT/farmacología , Útero/enzimología , Animales , Castración , Cicloheximida/farmacología , DDT/antagonistas & inhibidores , Dactinomicina/farmacología , Inducción Enzimática , Estradiol/farmacología , Femenino , Fructosa-Bifosfato Aldolasa/biosíntesis , Glucosafosfato Deshidrogenasa/biosíntesis , Glucógeno/metabolismo , Hexoquinasa/biosíntesis , Cinética , Tamaño de los Órganos , Ovario/fisiología , Fosfofructoquinasa-1/biosíntesis , Fosfogluconato Deshidrogenasa/biosíntesis , Progesterona/farmacología , Piruvato Quinasa/biosíntesis , Ratas , Espectrofotometría , Estereoisomerismo , Estimulación Química , Factores de Tiempo , Útero/anatomía & histología , Útero/efectos de los fármacos , Útero/metabolismoAsunto(s)
Dactinomicina/farmacología , Estradiol/farmacología , Glucosa-6-Fosfato Isomerasa/metabolismo , Fosfofructoquinasa-1/metabolismo , Útero/enzimología , Glándulas Suprarrenales/fisiología , Adrenalectomía , Animales , Femenino , Tamaño de los Órganos , Ovario/fisiología , Útero/análisis , Útero/efectos de los fármacosAsunto(s)
Antagonistas de Estrógenos , Fenobarbital/farmacología , Útero/enzimología , Animales , Castración , Femenino , Fructosa-Bifosfato Aldolasa/metabolismo , Glucosafosfato Deshidrogenasa/metabolismo , Hexoquinasa/metabolismo , Ovario , Fosfogluconato Deshidrogenasa/metabolismo , Piruvato Quinasa/metabolismo , Ratas , Útero/efectos de los fármacosRESUMEN
1. The hormonal regulation of phosphofructokinase was investigated in the accessory reproductive organs of the orchidectomized rat. 2. Phosphofructokinase activities declined to 51% and 47% in the prostate and 9% and 6% of the normal values in seminal vesicles 4 and 8 weeks after castration respectively. Administration of testosterone (100mug./100g. body wt.) for 3 days reversed substantially the effects of orchidectomy, and phosphofructokinase activity increased to 173% in the prostate and 536% in seminal vesicles as compared with the values of castrated controls. 3. Time-course studies demonstrated that after a single injection of testosterone (5mg./100g. body wt.) phosphofructokinase activity was maximally elevated to 236% in the prostate and 342% in seminal vesicles at 24hr. 4. Dose-response studies revealed that 2.5mg. of testosterone propionate/100g. body wt. was the minimal amount necessary to induce significant increases in enzyme activity in both accessory sex organs; maximal increases were obtained with a dose of 5mg./100g. body wt. 5. The observed enzyme increases induced by testosterone were inhibited by the simultaneous administration of oestradiol-17beta, and phosphofructokinase activity in this group of rats remained at 97% in the prostate and 137% of the control values in seminal vesicles. Oestradiol-17beta by itself failed to produce any significant effect on enzyme activity in either of these secondary sexual tissues. 6. The nature of the testosterone-induced increases in phosphofructokinase activity was studied by using a variety of inhibitors of RNA and protein synthesis. Cycloheximide, 5-fluorouracil and ethionine largely blocked the androgen-stimulated rise in enzyme activity observed 24hr. after steroid injection. The inhibitory effect of ethionine was completely reversed by the simultaneous administration of methionine. 7. Actinomycin, which is known to inhibit the synthesis of messenger RNA as well as the synthesis of other cellular RNA fractions, when given simultaneously with the hormone, also inhibited the testosterone-induced increases in prostatic and seminal-vesicular phosphofructokinase. However, when the antibiotic was given 6 or 12hr. after injection of the steroid, practically no inhibition of phosphofructokinase induction was obtained. This indicates that, once the enzyme-forming machinery is turned on and allowed to operate for a few hours, actinomycin is incapable of reversing the hormone-induced enzyme responses. 8. The results presented suggest that new RNA and protein synthesis may be involved in the observed androgen-induced increases in phosphofructokinase activity in the prostate and seminal vesicles of the orchidectomized rat.