Design, synthesis, biological evaluation of benzoyl amide derivatives containing nitrogen heterocyclic ring as potential VEGFR-2 inhibitors.

For the purpose of synthesizing drug candidates with desirable bioactivity, a class of benzoyl amide containing nitrogen heterocyclic ring derivatives targeting VEGFR-2 was designed and screened out using Discovery Studio. Eighteen target compounds were synthesized and then selected by some biological trials sequentially including inhibition of VEGFR-2, anti-proliferation in vitro, flow cytometry. Among them, compound 8h showed the best inhibitory activity (IC50 = 0.34 ±â€¯0.02 µM against VEGFR-2, IC50 = 1.08 ±â€¯0.06 µM and 2.44 ±â€¯0.15 µM against MCF-7 and HepG-2, respectively, which were at the same inhibitory level with the commercially antitumor drug: vandetanib). In addition, flow cytometry demonstrated that compound 8h induced MCF-7 cell apoptosis through a cell membrane-mediated pathway. This research highlights the therapeutic potential of novel VEGFR-2 inhibitors in treating cancers and provides a promising strategy for drug discovery.

Design, synthesis, and biological evaluation of pyrazole derivatives containing acetamide bond as potential BRAFV600E inhibitors.

With the increasingly acquired resistance, relapse and side effects of known marketed BRAFV600E inhibitors, it's significant to design the more effective and novel drugs. In this study, a series of novel pyrazole derivatives containing acetamide bond had been designed and synthesized on the basis of analysis of the endogenous ligands extracted from the known B-Raf co-crystals in the PDB database. Then, the compounds were evaluated for biological activities as potential BRAFV600E inhibitors. The bioassay results in vitro against three human tumor cell lines revealed that some of the compounds showed very impressed antiproliferative property. Among them, the compound 5r with IC50 values of 0.10 ±â€¯0.01 µM against BRAFV600E and 0.96 ±â€¯0.10 µM against A375 cell line, showed the most potent inhibitory effect, compared with the positive-controlled agents vemurafenib (IC50 = 0.04 ±â€¯0.004 µM for BRAFV600E, IC50 = 1.05 ±â€¯0.10 µM against A375). Further investigation confirmed that the compound 5r could induce A375 cell apoptosis, induce A375 cell death through changing mitochondrial membrane potential, and result in A375 cell arrest at the G1 phase of the cell cycle. Docking simulations results indicated that the compound 5r could bind tightly at the BRAFV600E active site. Meanwhile, 3D-QSAR model suggested that these compounds may be potential anticancer inhibitors. Overall, the article provided some new molecular scaffolds for the further BRAFV600E inhibitors.

[Characteristics of maxillary anterior teeth of 191 individuals living in Fujian Province].

PURPOSE: To investigate the anatomical characteristics of upper anterior teeth of residents in Fujian province using cone-beam CT (CBCT). METHODS: The length and width of 1146 maxillary anterior teeth (central incisors, lateral incisors, and canines) from 191 patients were measured. SPSS 19.0 software package was used to analyze the data. RESULTS: The width and length of males' maxillary central incisors and canine were significantly greater than those of females(P<0.05). No significant difference was found in the width and length of maxillary lateral incisors between genders (P>0.05). No significant difference was found in the width-length ratio of maxillary anterior teeth between genders(P>0.05). Significant difference was found in the width-width ratio of maxillary anterior teeth between genders(P<0.05). CONCLUSIONS: Varied anatomical characteristics of the maxillary anterior teeth of residents in Fujian Province should be observed between genders.