[Transformation Path of Clinical Research Achievements and Its Application in Medical Equipment Management under Background of Multi-agent Collaborative Innovation].

By studying the current situation of multi-agent collaborative innovation and clinical achievements transformation at home and abroad, it is clear that multi-agent collaborative innovation is the only way for clinical research achievements transformation under the current background. This paper proposes a set of transformation path of clinical research achievements based on the multi-agent collaborative innovation platform of "production, teaching, research and medicine", which is supported by policy guidance and innovation management, and explore the role of equipment management department in achievement transformation.

Inhibition of Usp14 ameliorates renal ischemia-reperfusion injury by reducing Tfap2a stabilization and facilitating mitophagy.

Mitochondrial dysfunction is recognized as a pivotal contributor to the pathogenesis of renal ischemia-reperfusion (IR) injury. Mitophagy, the process responsible for removing damaged protein aggregates, stands as a critical mechanism safeguarding cells against IR injury. Currently, the role of deubiquitination in regulating mitophagy still needs to be completely elucidated. This study aimed to evaluate the impact of ubiquitin-specific peptidase 14 (Usp14), a deubiquitinase, in IR injury by influencing mitophagy. Utilizing a murine model of renal IR injury, Usp14 silencing was found to ameliorate kidney injury, leading to decreased levels of serum creatinine and blood urea nitrogen, alongside diminished oxidative stress and inflammation. In renal epithelial cells subjected to hypoxia/reoxygenation (H/R), Usp14 knockdown increased cell viability and reduced apoptosis. Further mechanistic studies revealed that Usp14 interacted with and deubiquitinated transcription factor AP-2 alpha (Tfap2a), thereby suppressing its downstream target gene, TANK binding kinase 1 (Tbk1), to influence mitophagy. Tfap2a overexpression or Tbk1 inhibition reversed the protective effects of Usp14 silencing on renal tubular cell injury and its facilitation of mitophagy. In summary, our study demonstrated the renoprotective role of Usp14 knockdown in mitigating renal IR injury by promoting Tfap2a-mediated Tbk1 upregulation and mitophagy. These findings advocate for exploring Usp14 inhibition as a promising therapeutic avenue for mitigating IR injury, primarily by enhancing the clearance of damaged mitochondria through augmented mitophagy.

Robust quantitative single-exposure laser speckle imaging with true flow speckle contrast in the temporal and spatial domains.

A systematic and robust laser speckle contrast imaging (LSCI) method and procedure is presented covering the LSCI system calibration, static scattering removal, and measurement noise estimation and correction to obtain a true flow speckle contrast K f 2 and the flow speed from single-exposure LSCI measurements. We advocate the use of K 2 as the speckle contrast instead of the conventional contrast K, as the former relates simply to the flow velocity and is with additive noise alone. We demonstrate the efficacy of the proposed true flow speckle contrast by imaging phantom flow at varying speeds, showing that (1) the proposed recipe greatly enhances the linear sensitivity of the flow index (inverse decorrelation time) and the linearity covers the full span of flow speeds from 0 to 40 mm/s; and (2) the true flow speed can be recovered regardless of the overlying static scattering layers and the type of speckle statistics (temporal or spatial). The fundamental difference between the apparent temporal and spatial speckle contrasts is further revealed. The flow index recovered in the spatial domain is much more susceptible to static scattering and exhibit a shorter linearity range than that obtained in the temporal domain. The proposed LSCI analysis framework paves the way to estimate the true flow speed in the wide array of laser speckle contrast imaging applications.

Correlation analysis of metabolic syndrome and its components with thyroid nodules.

OBJECTIVE: This study aimed to analyze the relationship between the metabolic syndrome (MetS) and its components with the occurrence of thyroid nodules. METHODS: A total of 2719 volunteers from some areas of Gansu Province, China, who participated in the national survey of thyroid diseases and iodine nutrition status (Tide) and diabetes prevalence, were selected. Their height, weight, waist circumference (WC), systolic blood pressure (SBP), and diastolic blood pressure were recorded. The fasting plasma glucose (FPG), 2-h plasma postprandial glucose (2hPG), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and glycosylated hemoglobin (HbA1C) levels were measured. The prevalence of MetS and thyroid nodules was evaluated, and the correlation between each component of MetS and thyroid nodules was studied. RESULTS: The prevalence of MetS and thyroid nodules was 15.4% and 17.2%, respectively. WC, SBP, body mass index, FPG, 2hPG, TG, TC, and thyroid-stimulating hormone levels were significantly higher in the thyroid nodule group. The prevalence of thyroid nodules was significantly higher in the MetS group. A positive correlation was found between the degree of metabolic disorder and the occurrence of thyroid nodules. WC was found to be a risk factor for the occurrence of thyroid nodules. For WC≥90 cm, an increase in the independent variables led to a significant rise in the incidence of thyroid nodules. CONCLUSION: The prevalence of thyroid nodules was higher in the MetS group. The WC of the MetS components might be an independent risk factor for the occurrence of thyroid nodules.

Colorimetric recognition of 6-benzylaminopurine in environmental samples by using thioglycolic acid functionalized silver nanoparticles.

A simple and selective colorimetric sensor thioglycolic acid capped silver nanoparticles (TGA-AgNPs) was developed for the detection of 6-benzylaminopurine (6-BAP). The synthesized TGA-AgNPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), and transmission electron microscopic (TEM) techniques. The TGA-AgNPs as a sensor for binding 6-BAP through hydrogen-bonding and π-π bonding that causes large conjugate clusters, resulting in a color change from yellow to reddish orange. The surface plasmon resonance (SPR) band of TGA-AgNPs at 397nm is red-shifted to 510nm, which confirms that 6-BAP induces the aggregation of TGA-AgNPs. Under the optimized conditions, a linear relationship between the absorption ratio (A510nm/A397nm) and 6-BAP concentration was found in the range of 4-26µM. The detection limit of 6-BAP was 0.2µM, which is lower than the other analytical techniques. Moreover, the proposed sensor was successfully applied for the detection of 6-BAP in environmental samples with good recoveries. The proposed assay provides a simple and cost-effective method for the analysis of 6-BAP in vegetable and water samples.

Green synthesis of carbon dots functionalized silver nanoparticles for the colorimetric detection of phoxim.

In this work, Lycii Fructus as raw materials for green synthesis of fluorescent carbon dots (CDs) reduce AgNO3. The CDs-AgNPs were synthesized by one-step method. CDs were applied to stabilize AgNPs due to abundant functional groups on the surface of CDs. In presence of phoxim, the dispersed CDs-AgNPs get aggregated and the absorption peak with red shift from 400 nm to 525 nm, resulting in the color changed from yellow to red. Under optimized conditions, the absorbance ratio at A525 nm/A400 nm was related linearly to the concentrations of phoxim in the range of 0.1-100 µM. The detection limit was calculated to 0.04 µM, which is lower than maximum residue limits of phoxim in samples in China. The colorimetric sensor was successfully utilized to monitoring phoxim in environmental and fruit samples with good recoveries ranges from 87% to 110.0%. These results showed the sensor had a promising application prospect in real samples.

Silver nanoparticles-based colorimetric array for the detection of Thiophanate-methyl.

A simple and selective colorimetric sensor based on citrate capped silver nanoparticles (Cit-AgNPs) is proposed for the detection of Thiophanate-methyl (TM) with high sensitivity and selectivity. The method based on the color change of Cit-AgNPs from yellow to cherry red with the addition of TM to Cit-AgNPs that caused a red-shift on the surface plasmon resonance (SPR) band from 394nm to 525nm due to the hydrogen-bonding and substitution. The density functional theory (DFT) method was also calculated the interactions between the TM and citrate ions. Under the optimized conditions, a linear relationship between the absorption ratio (A525nm/A394nm) and TM concentration was found in the range of 2-100µM with correlation coefficient (R2) of 0.988. The detection limit of TM was 0.12µM by UV-vis spectrometer. Moreover, the applicability of colorimetric sensor is successfully verified by the detection of TM in environmental samples with good recoveries.

In vivo real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light.

We present the real-time single snapshot multiple frequency demodulation - spatial frequency domain imaging (SSMD-SFDI) platform implemented with a visible digital mirror device that is capable of imaging and monitoring dynamic turbid medium and processes over a large field of view. One challenge in quantitative imaging of biological tissue such as the skin is the complex structure rendering techniques based on homogeneous medium models to fail. To address this difficulty we have also developed a novel method that maps the layered structure to a homogeneous medium for spatial frequency domain imaging. The varying penetration depth of spatially modulated light on its wavelength and modulation frequency is used to resolve the layered structure. The efficacy of the real-time SSMD-SFDI platform and this two-layer model is demonstrated by imaging forearms of 6 healthy subjects under the reactive hyperemia protocol. The results show that our approach not only successfully decouples light absorption by melanin from that by hemoglobin and yields accurate determination of cutaneous hemoglobin concentration and oxygen saturation, but also provides reliable estimation of the scattering properties, the melanin content and the epidermal thickness in real time. Potential applications of our system in imaging skin physiological and functional states, cancer screening, and microcirculation monitoring are discussed at the end.