RESUMEN
Chronoamperometry was used to study the dynamics of Pt nanoparticle (NP) collision with an inert ultramicroelectrode via electrocatalytic amplification (ECA) in the hydrogen evolution reaction. ECA and dynamic light scattering (DLS) results reveal that the NP colloid remains stable only at low proton concentrations (1.0â mm) under a helium (He) atmosphere, ensuring that the collision events occur at genuinely single NP level. Amperometry of single NP collisions under a He atmosphere shows that each discrete current profile of the collision event evolves from spike to staircase at more negative potentials, while a staircase response is observed at all of the applied potentials under hydrogen-containing atmospheres. The particle size distribution estimated from the diffusion-controlled current in He agrees well with electron microscopy and DLS observations. These results shed light on the interfacial dynamics of the single nanoparticle collision electrochemistry.
RESUMEN
This study developed a novel electrochemical sensor containing nitrogen-doped ordered mesoporous carbon (NOMC) for the sensitive and selective quantification of l-tryptophan (Trp). The electro-oxidation mechanism of Trp on the NOMC/Nafion/glass carbon electrode (GCE) was first investigated, and was found to follow a two-electron/two-proton transfer mechanism. Subsequently, the analytical operation conditions were optimized. Under the optimum testing conditions, the oxidation current was found to increase linearly with Trp concentration in the ranges 0.5-70.0 µM and 70.0-200.0 µM (different slopes in each range), with the limit of detection determined to be 35.0 nM (S/N = 3). In addition, the sensor was highly selective for Trp and showed good repeatability and long-term stability. Studies of Trp in real world systems, such as an 18 amino acid mixture and an enzymatic protein hydrolysate, showed excellent recoveries (99.30-103.60%). Results suggest that NOMC/Nafion/GCE sensor has excellent performance characteristics for routine Trp analysis.