RESUMEN
Polymer hydrogels find extensive application in biomedicine, serving specific purposes such as drug delivery, biosensing, bioimaging, cancer therapy, tissue engineering, and others. In response to the growing threat of bacterial infections and the escalating resistance to conventional antibiotics, this research introduces a novel injectable, self-healing antimicrobial hydrogel comprising bioactive aldolized hyaluronic acid (AHA) and quaternized chitosan (QCS). This designed QCS/AHA hydrogel incorporates self-assembling peptide nanofibers (PNFs) and small-sized silver nanoparticles (AgNPs) for tailored functionality. The resulting hybrid QCS/AHA/PNF/AgNPs hydrogel demonstrates impressive rheological characteristics, broad-spectrum antimicrobial efficacy, and high biocompatibility. Notably, its antimicrobial effectiveness against Escherichia coli and S. aureus surpasses 99.9%, underscoring its potential for treating infectious wounds. Moreover, the rheological analysis confirms its excellent shear-thinning and self-healing properties, enabling it to conform closely to irregular wound surfaces. Furthermore, the cytotoxicity assessment reveals its compatibility with human umbilical vein endothelial cells, exhibiting no significant adverse effects. The combined attributes of this bioactive QCS/AHA/PNF/AgNPs hydrogel position it as a promising candidate for antimicrobial applications and wound healing.
Asunto(s)
Antibacterianos , Escherichia coli , Células Endoteliales de la Vena Umbilical Humana , Hidrogeles , Nanopartículas del Metal , Pruebas de Sensibilidad Microbiana , Nanofibras , Péptidos , Plata , Staphylococcus aureus , Plata/química , Plata/farmacología , Hidrogeles/química , Hidrogeles/farmacología , Hidrogeles/síntesis química , Antibacterianos/química , Antibacterianos/farmacología , Nanopartículas del Metal/química , Escherichia coli/efectos de los fármacos , Nanofibras/química , Humanos , Péptidos/química , Péptidos/farmacología , Staphylococcus aureus/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Quitosano/química , Cicatrización de Heridas/efectos de los fármacosRESUMEN
Amphiphilic peptides have garnered significant attention due to their highly designable and self-assembling behaviors. Self-assembled peptides hold excellent potential in various fields such as biosensing, environmental monitoring, and drug delivery, owing to their remarkable biological, physical, and chemical properties. While nanomaterials formed by peptide self-assembly have found widespread use in biomedical applications, the development of 2D peptide nanosheets based on the self-assembly of amphiphilic peptides remains challenging in terms of rational design and morphology modulation. In this study, rationally designed amphiphilic peptide molecules are self-assembled into peptide nanosheets (PNS) under specific conditions to encapsulate gold nanoparticles (AuNPs), resulting in the formation of AuNPs/PNS hybrid materials with high photothermal conversion efficiency. The findings demonstrate that 2D PNS enhances the overall photothermal therapy effect of the nanohybrid materials due to their larger hosting area for AuNPs and higher biocompatibility. The well-designed amphiphilic peptides in this study offer insights into the structural design and functional modulation of self-assembled molecules. In addition, the constructed biomimetic-functional 2D inorganic/organic nanohybrid materials hold potential applications in biomedical engineering.
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Biomimetic synthesis provides potential guidance for the synthesis of bio-nanomaterials by mimicking the structure, properties and functions of natural materials. Behavioral studies of biological surfaces with specific micro/nano structures are performed to explore the interactions of various molecules or organisms with biological surfaces. These explorations provide valuable inspiration for the development of biomimetic surfaces with similar effects. This work reviews some conventional preparation methods and functional modulation strategies for biomimetic interfaces. It aims to elucidate the important role of biomimetic interfaces with antifouling and low-pollution properties that can replace non-environmentally friendly coatings. Thus, biomimetic antifouling interfaces can be better applied in the field of marine antifouling and antimicrobial. In this review, the commonly used fabrication methods for biomimetic interfaces as well as some practical strategies for functional modulation is present in detail. These methods and strategies modify the physical structure and chemical properties of the biomimetic interfaces, thus improving the wettability, adsorption, drag reduction, etc. that they exhibit. In addition, practical applications are presented of various biomimetic interfaces for antifouling and look ahead to potential biomedical applications. By continuously discovering functional surfaces with biomimetic properties and studying their microstructure and macroscopic properties, more biomimetic interfaces will be developed.
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Fluorescent bioimaging and photothermal therapy (PTT) techniques have potential significance in cancer diagnosis and treatment and have been widely applied in biomedical and practical clinical trials. This study proposes the molecular design and biofabrication of a two-dimensional (2D) nanoplatform, exhibiting promising prospects for synergistic bioimaging and PTT of tumors. First, biocompatible 2D peptide nanosheets (PNSs) were designed and prepared through peptide self-assembly. These served as a support matrix for assembling polyethylene glycol-modified Ag2S quantum dots (PEG-Ag2SQDs) to form a 2D nanoplatform (PNS/PEG-Ag2SQDs) with unique fluorescent and photothermal properties. The designed 2D nanoplatform not only showed improved photothermal efficacy and an elevated photothermal conversion efficiency of 52.46 %, but also demonstrated significant lethality against tumors in both in vitro and in vivo cases. Additionally, it displays excellent imaging effects in the near-infrared II region, making it suitable for synergistic fluorescent imaging-guided PTT of tumors. This study not only provides a facile approach for devising and synthesizing 2D peptide assemblies but also presents new biomimetic strategies to create functional 2D organic/inorganic nanoplatforms for biomedical applications.
Asunto(s)
Nanopartículas , Neoplasias , Puntos Cuánticos , Humanos , Fototerapia/métodos , Terapia Fototérmica , Nanopartículas/química , Biomimética , Neoplasias/diagnóstico por imagen , Neoplasias/terapia , Neoplasias/patología , Péptidos , Línea Celular TumoralRESUMEN
Objective: To explore the equivalence of an easier and more convenient lumbar multifidus (LM) muscle exercise pattern among standing back-extension, static standing, and superman training. Methods: A total of 26 healthy young volunteers were enrolled, including 14 males and 12 females, aged from 22 to 44 years with an average of 31.77 ± 7.06 years. Ultrasonography was used to measure the thickness of the left LM of the transverse process of the L5 vertebra during static standing, static prone decubitus, standing back-extension, and prone superman training. In this study, measurement data were expressed as Mean ± SD and compared using the t-test. Results: The left LM thickness of the L5 vertebra was 2.92 ± 0.46 cm during static standing and 2.78 ± 0.39 cm during static prone decubitus, showing no statistical difference between the two groups (P > 0.05). The left LM thickness of the L5 vertebra was 3.16 ± 0.51 cm during standing back-extension and 3.33 ± 0.41 cm during the prone superman training, indicating no statistical difference between the two groups (P > 0.05). Conclusions: There is no significant statistical difference in the LM thickness between static standing and static prone decubitus and between standing back-extension and prone superman training, indicating the equivalence of the two methods in LM exercise, providing a simpler and easier way for clinical exercise of lumbodorsal muscles.
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Ejercicio Físico , Músculos Paraespinales , Adulto , Ejercicio Físico/fisiología , Femenino , Humanos , Región Lumbosacra/diagnóstico por imagen , Masculino , Músculos Paraespinales/diagnóstico por imagen , Músculos Paraespinales/fisiología , Posición de Pie , Ultrasonografía , Adulto JovenRESUMEN
ABSTRACT: The influencing factors in closed reduction internal fixation with cannulated screw of femoral neck fractures have not been well investigated. This study evaluated these factors in patients with femoral neck fractures.Fifty-seven patients (36 males and 21 females) diagnosed with femoral neck fracture with the average age of 52.44â±â15.04 years who underwent closed reduction internal fixation with cannulated screw were included in this study. Data were collected through case report reviews, phone call follow-ups, and outpatient follow-ups to evaluate pre- and postoperative radiograph images. Statistical analysis was performed using Garden classification, binary and multinomial logistic regression analysis by including factors such as patient's age, gender, fracture type, time to fixation, reduction quality, functional recovery period, removal of cannulated screw, and preoperative traction. Logistic regression analysis revealed that age and reduction quality was statistically significant (Pâ<â.05) to clinical outcome and other factors were not statistically significant.The main factors affecting clinical outcomes were functional recovery and reduction quality. The biomechanical effects of fixation provide a good foundation for fracture healing. Patient's conditions should be carefully evaluated before selecting reduction procedures to reach an optimal surgical outcome.