ACC1-mediated fatty acid biosynthesis intrinsically controls thymic iNKT cell development.

To meet the energetic requirements associated with activation, proliferation, and survival, T cells switch their metabolic signatures from energetically quiescent to activated. However, little is known about the role of metabolic pathway controlling the development of invariant natural killer T (iNKT) cells. In the present study, we found that acetyl-CoA carboxylase 1 (ACC1), a rate-limiting enzyme for the fatty acid biosynthesis pathway, plays an essential role in the development of iNKT cells in the thymus. Mice lacking T-cell specific ACC1 showed a reduced number of iNKT cells with an increased proportion of iNKT cells at immature stages 0 and 1. Furthermore, mixed bone marrow (BM) chimera experiments revealed that T-cell intrinsic ACC1 expression was selectively important for the development of thymic iNKT cells, especially for the differentiation of the NKT1 cell subset. Our single-cell RNA-sequencing (scRNA-seq) data and functional analysis demonstrated that ACC1 is responsible for survival of developing iNKT cells. Thus, these findings highlighted a novel role of ACC1 in controlling thymic iNKT cell development mediated by the control of cell survival.

[Development of a scale to evaluate the rest levels of male workers].

Objectives　"Rest" has two aspects: "repose," to recover from physical or mental fatigue, and "restore," to re-establish physical, mental, and social health. Many male workers work for prolonged hours, and despite stress, they do not apply coping behaviors, unlike female workers. Consequently, taking sufficient rest is difficult for male workers. The aim of this study was to develop a scale incorporating multiple factors to evaluate the state in which male workers take rest.Methods　A questionnaire survey was conducted involving 330 male workers aged 20-59 years who had been engaged in two manufacturing industries. The questionnaire items were created with reference to an interview survey of male workers in the literature. After discussing with industrial hygiene experts and conducting a pretest to obtain surface validity, 70 items were used for analyses. In the exploratory factor analysis, promax rotation was used with the maximum likelihood method. In the confirmatory factor analysis, goodness of fit was confirmed with the covariance structure analysis. Health-related quality of life, job stress, work engagement, sleep, and self-assessment of rest were used to examine concurrent validity.Results　With the exploratory factor analysis, we introduced a scale containing 15 items, including three subscales: filling with spirit, enthusiasm for work, and recovery from fatigue. The reliability coefficient (Cronbach's alpha) of the three subscales and the overall scale ranged from 0.79 to 0.88 and 0.89, ensuring internal consistency. The confirmatory factor analysis showed a generally good fit. Their scores were correlated with many items of the health-related quality of life, job stress, work engagement, sleep, and self-assessment of rest.Conclusions　A 15-item "Rest Evaluation Scale" was created to evaluate the state in which male workers in the manufacturing industries take rest. Since the validity, reliability, and concurrent validity of this scale were satisfactory, this scale was considered to be an index evaluating the state of rest of male workers.

[The associations of Japan's specific medical checkup with a health promotion lifestyle, health literacy, and social capital by healthcare coverage type].

Objectives　The goal of this study was to determine whether experiencing Japan's specific medical checkup is associated with a health promotion lifestyle, health literacy (HL), and social capital (SC) by type of healthcare coverage.Methods　The subjects were 1,048 residents of A City aged 40 through 74 years. Individuals who had experienced more than one medical checkup during the past two years were categorized as "Consultation." Individuals without that experience were categorized as "No consultation." Data were collected via an anonymous self-administered mail questionnaire survey. Health promotion lifestyles were assessed using a Japanese version of the Health-Promoting Lifestyle Profile (HPLP II). HL was measured with the 14-item Health Literacy Scale. The SC was measured following Hanibuchi's six-item scale.Results　The response rates were 34.4% for males and 39.6% for females. Of the males covered by National Health Insurance (NHI), 68.8% were Consultation. Of the females covered by NHI, 79.4% were Consultation. Of the males covered by social insurance, 91.7% were Consultation, and 72.6% of the females covered by social insurance were Consultation. The consultation ratio of males covered by NHI was significantly lower among those without a co-residential spouse than among those living with a spouse. The physical activity score (a HPLP II subscale) of the females covered by NHI was significantly lower among those without compared to those with consultation. Among the males covered by social insurance, the nutrition score (a HPLP II subscale) was significantly higher among those without compared to those with consultation, and the number of neighbors providing companionship (an item in the SC scale) was larger. Among the females covered by social insurance, the health responsibility score (a HPLP II subscale) was significantly lower among those without than among those with consultation.Conclusion　There were significant relationships between experiencing more than one medical checkup during the past two years and health promotion lifestyles and between that experience and social capital, except for males covered by NHI. Policies based on these results are encouraged to increase participation in medical checkups. Regarding males covered by NHI, support of their participation in medical checkups by people close to them would be an effective way to increase participation.

[Characteristics of health literacy, social capital, and health behavior acquired through experiences by health promotion volunteers].

Objectives　In order to promote health in the community, collaborative activities with community organizations are, in addition to individual health guidance, considered to be effective. Health promotion volunteers (HPVs) are now gaining attention as one such community organization. The purposes of this research were to clarify the characteristics of health literacy, social capital, and health behavior acquired through experience by HPVs and to obtain findings with which to examine ways to foster HPVs.Methods　An anonymous, self-administered questionnaire survey was conducted through the postal system in a town with 87 currently active HPVs, 158 former HPVs who served between April 2009 and March 2015, and 299 people with no experience as HPVs. The respondents were 54 active HPVs (response rate, 62.1%); 69 former HPVs (43.7%); and 136 people with no experience (45.5%). Participants were asked about their sociodemographic characteristics; activity status as an HPV; self-rated health; whether they had undergone a medical examination (recently) or not; health literacy; social capital; health behaviors; etc.Results　Health literacy, social capital, and health behavior scores of active HPVs showed no significant difference among the three groups. Many of the active HPVs felt that the work was "of help to the local government." The number of participants who tried to encourage others and who self-rated their health statuses as healthy in the active HPV group were significantly higher compared with the former HPVs and the group with no experience.Conclusion　In fostering HPVs, it is necessary to support them in order to ensure that they proactively engage in activities promoting health.

Enzymatic and structural characterization of an archaeal thiamin phosphate synthase.

Studies on thiamin biosynthesis have so far been achieved in eubacteria, yeast and plants, in which the thiamin structure is formed as thiamin phosphate from a thiazole and a pyrimidine moiety. This condensation reaction is catalyzed by thiamin phosphate synthase, which is encoded by the thiE gene or its orthologs. On the other hand, most archaea do not seem to have the thiE gene, but instead their thiD gene, coding for a 2-methyl-4-amino-5-hydroxymethylpyrimidine (HMP) kinase/HMP phosphate kinase, possesses an additional C-terminal domain designated thiN. These two proteins, ThiE and ThiN, do not share sequence similarity. In this study, using recombinant protein from the hyperthermophile archaea Pyrobaculum calidifontis, we demonstrated that the ThiN protein is an analog of the ThiE protein, catalyzing the formation of thiamin phosphate with the release of inorganic pyrophosphate from HMP pyrophosphate and 4-methyl-5-ß-hydroxyethylthiazole phosphate (HET-P). In addition, we found that the ThiN protein can liberate an inorganic pyrophosphate from HMP pyrophosphate in the absence of HET-P. A structure model of the enzyme-product complex of P. calidifontis ThiN domain was proposed on the basis of the known three-dimensional structure of the ortholog of Pyrococcus furiosus. The significance of Arg320 and His341 residues for thiN-coded thiamin phosphate synthase activity was confirmed by site-directed mutagenesis. This is the first report of the experimental analysis of an archaeal thiamin synthesis enzyme.

Effects of bifidobacterial supplementation to pregnant women and infants in the prevention of allergy development in infants and on fecal microbiota.

BACKGROUND: Probiotic administration may be a useful method for preventing allergies in infants; however, there have been controversial results about the efficacy. We investigated the effects of bifidobacterial supplementation on the risk of developing allergic diseases in the Japanese population. METHODS: In an open trial, we gave Bifidobacterium breve M-16V and Bifidobacterium longum BB536 prenatally to 130 mothers beginning 1 month prior to delivery and postnatally to their infants for 6 months. Another 36 mother-infant pairs served as controls and did not receive the bifidobacterial supplementation. Development of allergic symptoms in the infants was assessed at 4, 10 and 18 months of age. Fecal samples were collected from the mothers and infants. RESULTS: The risk of developing eczema/atopic dermatitis (AD) during the first 18 months of life was significantly reduced in infants in the probiotic group (OR: 0.231 [95% CI: 0.084-0.628] and 0.304 [0.105-0.892] at 10 and 18 months of age, respectively). Pyrosequencing analyses indicated an altered composition of the fecal microbiota at 4 months for infants who developed eczema/AD at 4 and 10 months of age. The proportion of Proteobacteria was significantly lower (P = 0.007) in mothers at the time of delivery who received the supplementation when compared with the control group and was positively correlated (r = 0.283, P = 0.024) with that of infants at 4 months of age. No adverse effects were related to the use of probiotics. CONCLUSIONS: These data suggest that the prenatal and postnatal supplementation of bifidobacteria is effective in primary preventing allergic diseases. Some limited changes in the composition of fecal microbiota by the bifidobacterial supplementation were observed.

Association between teaching and support skills and subjective effectiveness of nutritional guidance of registered dietitians at hospitals in a Japanese prefecture.

OBJECTIVE: The aim of this study is to clarify the association between teaching and support skills and the subjective effectiveness of nutritional guidance of registered dietitians working at hospitals. METHODS: We carried out a questionnaire survey of registered dietitians at hospitals in a Japanese prefecture. The utilization of nutritional teaching skills in nutritional guidance was investigated using a self-produced 36-item questionnaire that was designed to be mainly used for diabetic patients in 4 settings: first guidance, first assessment, contemplation stage, and preparation stage. The support skills were evaluated by Kikuchi's Scale of Social Skills: 18 items. The subjective effectiveness of nutritional guidance was defined by the behavioral change of the patients after nutritional guidance as evaluated by a registered dietitian. RESULTS: There were 75 respondents (response rate 46.6 %). Among the teaching skills, basic skills in an interview were often used, but some related to coaching skills were not in common use in nutritional guidance. Based on the results of principal component analysis, we created a scale for scoring the utilization of nutritional teaching skills in each setting. Multiple linear regression analysis illustrated that high subjective effectiveness of nutritional guidance was associated with high score of teaching skills in the preparation stage setting and high score of support skills. CONCLUSIONS: These results show that, in addition to frequent use of nutritional teaching skills, improvement of support skills is also necessary to enhance the effectiveness of nutritional guidance.

Social development conditions and related factors for children in Japanese kindergarten in Shanghai, China.

OBJECTIVE: The aim of this study is to clarify the social development conditions and related factors for children in Japanese kindergarten in Shanghai, China. METHODS: The target schools were three Japanese kindergartens in Shanghai, three in Wakayama, Japan, and three in Osaka, Japan. The target kindergarten children were all 5- or 6-year-olds. We used an anonymous questionnaire to the guardian of the child. The question items related to social development, lifestyle and daily activities of the child, and rearing awareness and conditions of the guardian. Social development was scored by Strength and Difficulties Questionnaires (SDQ). RESULTS: The number of respondents was 246 (Shanghai group 72, Wakayama group 86, Osaka group 88). The median value of the score of total difficulties and prosocial behavior, SDQ subscale, and the proportion of children judged as low need showed no significant difference. Results of multiple logistic regression analysis showed that the frequency of eating fast-foods and the rearing condition "I become anxious or lose enthusiasm for childrearing when the child is recalcitrant" were the factors relevant to prosocial behavior in the Shanghai group. In the Wakayama group, the hours spent daily watching TV was designated as the relevant factor. In the Osaka group, hours spent daily playing outdoors was found to be the relevant factor. CONCLUSIONS: Social development of children in Japanese kindergarten in Shanghai was the same as that of those living in Japan. The factors relevant to prosocial behavior in the Shanghai group were different from those in the two cities in Japan. This study may show the important points to keep in mind for childrearing abroad.

Clinical practice in management of hydration for lung cancer patients receiving cisplatin-based chemotherapy in Japan: a questionnaire survey.

A questionnaire survey was performed to investigate the actual hydration methods used with cisplatin-containing regimens at various institutions in Japan to gain an overview of the varieties employed. Replies were received from 368 of 686 institutions board-certified by the Japanese Respiratory Society. In 233 institutions (63%), new lung cancer patients were treated regularly with regimens containing cisplatin at ≥60 mg/m2. In 172 institutions (48%), hydration with <3000 ml of intravenous saline was performed on day 1. In 225 institutions (65%), hydration was performed for up to 3 days at most, but no more than 48 (14%) of the institutions that responded did so on day 1 only. Two to three weeks of hospitalization was needed for the initial course at most institutions (76%). Thirteen institutions (4%) treated patients as outpatients after the second course, whereas none did so from the beginning of treatment. Despite inconsistencies among the methods used by the various institutions, 84% of those surveyed considered their approaches to be appropriate. Some useful objective indices for deciding the volume or duration of hydration are needed.

[Physical and mental features of elderly persons who experienced group exercise for care prevention].

OBJECTIVES: This study was conducted to clarify the physical and mental features of elderly persons who experienced group exercise for care prevention. METHOD: A group consisting of 92 elderly persons who had finished the group exercise program, one of the care prevention projects, in a town was surveyed (the experienced group) by anonymous self-administrative questionnaire. One hundred twenty-three elderly persons who had been attending community meetings were used as the control group. RESULT: Valid responses were obtained from 66 persons in the experienced group (valid response rate: 71.7%) and 102 persons in the control group (valid response rate: 82.9%). Fifty-nine sex-and age-matched persons from each of the groups were selected for the analysis. Significant differences were not observed in the attributes between the two groups. In 4 of 5 items of Instrumental Activities of Daily Living, the percentages of respondents who answered "possible to do alone" were significantly higher in the experienced group. In 6 of 14 items of "painful activities of daily life", the percentages of respondents were significantly lower in the experienced group. In 3 of 20 items on "relation with society", the scores were significantly higher in the experienced group. More persons actively went out or participated in social events in the experienced group. In the items of depression, the scores were significantly lower in the experienced group. There were significantly more persons who exercised daily in the experienced group. CONCLUSIONS: It was easy to expect that the experienced group can maintain their functions in daily life and their physical and mental activities. Thus, it is suggested that group exercise has good effects on elderly persons in terms of care prevention.

[Trends of Implementation of Nonsmoking at Eating and Drinking Establishments One Year before and after April 2020: Consideration of the Status of Implementation of the Amendment Bill for the Health Promotion Act of Japan].

OBJECTIVE: In this study, we aimed to clarify the transition to the implementation of smoking prohibition at eating and drinking establishments one year before and after April 2020, the time when they became "nonsmoking" in principle following the implementation of the amendment bill for the Health Promotion Act of Japan. METHODS: The total number of nonsmoking/smoking eating and drinking establishments by industry were obtained using the data from "Tabelog®." The number of people who tested positive for COVID-19 by the polymerase chain reaction test on the day of the survey nationwide and the bankruptcy status of the companies (eating and drinking establishments, etc.) for each month were ascertained. RESULTS: In 2020, a state of emergency was declared owing to the increase in the number of people positive for COVID-19, and many eating and drinking establishments went bankrupt. Despite these circumstances, the number of nonsmoking eating and drinking establishments exceeded that of smoking establishments in March 2020 and continued to increase thereafter. Additionally, the number of nonsmoking "restaurants" increased and exceeded that of smoking restaurants in June 2020. The number of nonsmoking "cafes" already exceeded that of smoking "cafes" at the beginning of this survey and continued to increase. The number of nonsmoking "bars" increased, but that of smoking "bars" remained high. CONCLUSION: It is necessary to promote measures against passive smoking while paying attention to the trends for different types of eating and drinking establishments, rather than considering all establishments together.

[Association of Annual Transition of Implementation of Nonsmoking at Eating and Drinking Establishments with Indices on Population/Household and Economy/Labor: Examination Using Prefectural Data].

OBJECTIVES: The purpose of this study was to clarify the relationship of the annual transition of implementation of nonsmoking at eating and drinking establishments with indices of population/household and economy/labor by prefecture. METHODS: The prefectural rates of eating and drinking establishments implementing nonsmoking (hereafter, nonsmoking rate) were computed in a year using the data from "Tabelog®". Forty-seven prefectures were classified by hierarchical cluster analysis into "prefecture clusters" 1 to 5 in descending order of the median of nonsmoking rates. The indices of population/household (e.g., percentage of the population aged 65 years and over and percentage of nuclear family household) and economy/labor (e.g., prefectural income per capita and percentage of construction and mining workers) were classified by hierarchical cluster analysis into 11 "index clusters", and the representative index in each index cluster was extracted from the results of the Jonckheere-Terpstra test. An ordinal logistic regression analysis was performed using the numbers 1 to 5 of prefecture clusters as dependent variables and the indices representing the index clusters as independent variables. RESULTS: The percentage of the population aged 65 years and over and the percentage of construction and mining workers were positively related to the order of prefectural clusters. CONCLUSIONS: To promote implementation of nonsmoking in eating and drinking establishments in prefectures especially in those with larger numbers of elderly people and construction and mining workers, it is important to inform the persons in charge that implementation of nonsmoking does not affect the number of customers.

Comparison of long-term changes in size and longevity of bee colonies in mid-west Japan and Maui with and without exposure to pesticide, cold winters, and mites.

Four long-term field experiments in mid-west Japan (Shika) made it clear that extinction of colonies exposed to neonicotinoid was much higher than for colonies exposed to organophosphates. The incidence of hive death for of organophosphate-exposed and control (pesticide-free) colonies was similar. We conducted a field experiment in Maui for 271 days using the same pesticides (dinotefuran: 0.2 ppm, clothianidin: 0.08 ppm, fenitrothion: 1 ppm) as used in Shika with the honeybee, Apis mellifera, colonies without mites. Numbers of adult bees, capped brood, mites and other hive parameters were accurately counted on photographs of combs and on the inside of the hives. All six neonicotinoid (dinotefuran & clothianidin)-exposed colonies failed during the experiment. One of three organophosphate (fenitrothion)-exposed colonies and one of the three control colonies also failed. The findings from Maui, where colonies displayed no mites, provides evidence from Shika, with mites, that neonicotinoids are more hazardous to honeybee colonies than organophosphates. The apparent longevity of honeybee colonies on Maui was estimated by numbers of adult bees and capped brood using a mathematical model previously proposed. Seasonal changes in longevity on Maui differ greatly from changes at Shika, the latter showing distinct seasonal variation. Longevity on Maui remains nearly constant throughout the year with wide variations. At Shika, it increases drastically in winter, by six- to ten fold more than the other seasons. Differences seem to depend on the existence of cold winters and the length of flowering seasons. In a perpetually hospitable environment, small changes in conditions can be sensitively reflected in apparent longevity. Examining wide variations in apparent longevity that are seemingly incoherent, we recognized several differences in apparent longevity between neonicotinoid-exposed and organophosphate-exposed colonies: The colony that failed in after organophosphate-exposure colony group exhibited the longest apparent longevity and the fewest number of newly capped brood, as also was the case in control colonies. Extended longevity when few brood are newly produced is reasonable to maintain the colony from a physiological point of view. Extension of apparent longevity is not seen in neonicotinoid-exposed colonies when the number of newly capped brood is fewer. This finding suggests that neonicotinoid pesticides may inhibit normal apian physiology.

A mathematical model to estimate the seasonal change in apparent longevity of bee colony.

The longevity of a honeybee colony is far more significant than the lifespan of an individual honeybee, a social insect. The longevity of a honeybee colony is integral to the fate of the colony. We have proposed a new mathematical model to estimate the apparent longevity defined in the upper limit of an integral equation. The apparent longevity can be determined only from the numbers of adult bees and capped brood. By applying the mathematical model to a honeybee colony in Japan, seasonal changes in apparent longevity were estimated in three long-term field experiments. Three apparent longevities showed very similar season-changes to one another, increasing from early autumn, reaching a maximum at the end of overwintering and falling approximately plumb down after overwintering. The influence of measurement errors in the numbers of adult bees and capped brood on the apparent longevity was investigated.

[Factors Related to Salt-Reduction Cognizance and Salt Intake in Women Aged 40-59 Years: Characteristics of Guardians of Medical University Students].

OBJECTIVES: In our previous study in which we aimed to clarify the factors related to salt intake in women aged 40-59 years, salt intake was found to be not related to salt-reduction cognizance. The aim of this research was to clarify factors related to salt intake in those who were cognizant of the importance of reducing their salt intake. METHODS: Two hundred and forty-seven female guardians (effective rate, 32.2%) in a medical university, aged 40-59 years old, participated in this study. The participants were divided into three groups according to their salt-reduction cognizance. RESULTS: There was no significant difference in salt intake between the three groups who were salt-reduction cognizant. Intakes of potassium (mg/1,000 kcal), vegetables, and fruits were higher in those who were cognizant of the importance of reducing their salt intake. The frequencies of consuming stewed foods, miso soup, and vinegared and marinated dishes were also higher. Those who were salt-reduction cognizant were knowledgeable about salt consumption, had experienced making low-salt dishes, used low-sodium seasoning, and made light-tasting dishes by regulating ingredients when cooking. However, when it came to eating, there was no difference in the percentage of those who left most of the broth when eating noodle soups and the frequency with which they added seasoning in terms of salt-reduction cognizance. CONCLUSION: Salt-reduction cognizant women aged 40-59 years made conscious efforts to use less salt at the time of cooking, but made no efforts when eating, even though they were cognizant of the importance of reducing their salt intake.

Health-promoting lifestyles of Japanese expatriates residing in the Philippines and Thailand.

BACKGROUND: The number of Japanese expatriates has increased, many of whom undertake lifestyle changes to improve their health. PURPOSE: We aim to clarify the health-promoting lifestyles of middle and older age Japanese expatriates. METHODS: This is a cross-sectional study. Participants comprised 95 long-term Japanese expatriates residing in popular destinations: the Philippines and Thailand. Health behavior was evaluated using a Japanese version of Health-Promoting Lifestyle Profile II and participants were compared with a group living in Japan. Multiple linear regression analyses were used to examine the factors that correlate with the score of physical activity, a Health-Promoting Lifestyle Profile II subscale. RESULTS: The expatriates had significantly higher scores of physical activity and nutrition than the group living in Japan. Factors positively related to physical activity were communicative literacy, information on health management before leaving Japan, no history of hypertension, and sufficient English proficiency. CONCLUSION: The Japanese expatriates we studied were physically active and were careful about nutrition. To further increase their physical activity, better access to health information and enhancement of communicative literacy and of English proficiency are desired.

[Association of the Status of Implementation of Nonsmoking at Eating and Drinking Establishments with Prevalence of Persons with Subjective Symptoms, Prevalence of Persons with Diseases under Treatment, Medical Expenses, and Mortality Rate: Examination Using Prefectural Data].

OBJECTIVES: The purpose of this study was to confirm the association of the status of implementation of nonsmoking at eating and drinking establishments with the prevalence of persons with subjective symptoms, the prevalence of persons with diseases under treatment, medical expenses, and mortality rate using prefectural data. METHODS: The prefectural rate of eating and drinking establishments implementing nonsmoking (hereafter, nonsmoking rate) was calculated using the data from "Tabelog®". The variables of interest were the prevalence of persons with subjective symptoms, the prevalence of persons with diseases under treatment, medical expenses (total, hospitalization and nonhospitalization expenses), and the mortality rates of malignant neoplasms (lung cancer, stomach cancer, and colon cancer), heart disease, acute myocardial infarction, cerebrovascular disease, cerebral infarction, and pneumonia in each prefecture. The partial correlation coefficient was estimated between the nonsmoking rate and the variable of interest using the smoking rate by prefectural as the control variable. RESULTS: The nonsmoking rate showed a significantly negative correlation with the medical expenses. When eating and drinking establishments were divided into "restaurant", "café", and "bar", the nonsmoking rate also indicated a significantly negative correlation with the medical expenses in any category. It was negatively related to the mortality rates of cerebrovascular disease, cerebral infarction, and pneumonia. The negative correlation was stronger in females than in males. CONCLUSIONS: These results suggest that the implementation of nonsmoking at eating and drinking establishments may reduce the mortality rates of diseases, such as cerebrovascular disease, cerebral infarction, and pneumonia, and medical expenses. Thus, it is important to implement nonsmoking at eating and drinking establishments in line with the Revised Health Promotion Act.

The direct precursor of the pyrimidine moiety of thiamin is not urocanic acid but histidine in Saccharomyces cerevisiae.

The biosynthetic route of the pyrimidine moiety of thiamin is different in prokaryotes and eukaryotes. In prokaryotes, the pyrimidine moiety is synthesized from aminoimidazole ribonucleotide, an intermediate of purine biosynthesis, while in eukaryotes, we have reported that the N-1, C-2, and N-3 atoms of the imidazole ring of histidine are incorporated into N-3, C-4, and the amino group attached to the C-4 atoms of the pyrimidine moiety, respectively, as a unit; the rest of the atoms of the pyrimidine moiety originate from pyridoxine as a unit. It has been reported that urocanic acid, the deaminated compound of histidine, is the direct precursor of the pyrimidine moiety. In the present report, we have investigated whether histidine or urocanic acid is the direct precursor of the pyrimidine moiety in Saccharomyces cerevisiae, using tracer experiments with 1) (13)C-formate and urocanic acid, 2) (15)N-NH(4)Cl and urocanic acid, 3) (15)N-NH(4)Cl and histidine, and 4) (13)C-histidine and urocanic acid. The GC-MS analysis revealed that the incorporation of the (15)N atom of (15)NH(4)Cl was not affected by the presence of urocanic acid, although it was affected by histidine, and the incorporation of (13)C-histidine was not affected by the presence of urocanic acid. These results confirm that histidine is the direct precursor of the pyrimidine moiety of thiamin in S. cerevisiae.

[Conditions of Food Consumption Related to Salt Intake in Women Aged 40-59 Years: Characteristics in Working Mothers of Medical University Students].

OBJECTIVES: The aims of this study were to clarify the conditions of food consumption related to salt intake in women aged 40-59 years and to determine the characteristics of working mothers. METHODS: Among all guardians in a medical university, 247 (effective response rate, 32.2%) mothers of 40-59 years of age participated in this study. RESULTS: Factors related to salt intake were the frequency of consuming Japanese dishes (stewed foods, Japanese style rice, pickles, miso soup, noodles, vinegared dishes, marinated foods) and dietary habit (use of low-sodium seasoning when cooking, the amount of broth left unconsumed when eating noodle soup, the use of seasoning at the table). In comparison with the nonworking mothers, a greater number of the working mothers purchased smaller amount of fresh food and prepared meals for dinner in less than 30 minutes. Furthermore, the frequency of consuming Japanese dishes was also lower in the working mothers. This was not related to their state of mind or knowledge about salt consumption in relation to health and conscious effort to eat less salt, but rather to their longer work hours and shorter meal preparation time. Dietary habits are related to the state of mind, flavor, and knowledge and efforts to eat less salt. Working mothers, as well as nonworking mothers had dietary habits. CONCLUSION: For women in this age group, to reduce salt consumption, adopting ways to eat less salt at the table, which are feasible for everyone, are effective.

[Health Effects Accompanying the Transition from Cigarettes to Heat-not-burn Tobacco: Nicotine Dependence, Nicotine Withdrawal Symptoms, and Changes in Smoking Behaviors].

OBJECTIVES: The purposes of this research were to investigate the hazardous effects of heat-not-burn tobacco and to clarify the health effects accompanying the transition from cigarettes to heat-not-burn tobacco. METHODS: The concentrations of carbon monoxide, ammonia, formaldehyde, acetaldehyde, and dust (hazardous substances) were measured in the smoke of heat-not-burn tobacco. Twenty-nine smokers were used as the subjects. The concentrations of hazardous substances were measured in exhalation of heat-not-burn tobacco. The concentration of cotinine in saliva was also measured after the transition. A questionnaire survey was performed before and after the transition to evaluate nicotine dependence, nicotine withdrawal symptoms, and smoking behaviors. RESULTS: In the mainstream smoke, all hazardous substances investigated were detected. Carbon monoxide and dust were detected in the exhalation of heat-not-burn tobacco. The concentration of cotinine in the saliva of heat-not-burn tobacco users corresponded to that of cigarette smokers. Cigarette smoking was significantly positively related to the score of Fagerström Test for Nicotine Dependence (FTND). Heat-not-burn tobacco was significantly positively related to FTND and Minnesota Nicotine Withdrawal Scale (MNWS) scores. The group in which the number of heat-not-burn tobacco sticks consumed increased after transition showed a smaller number of cigarettes consumed and a higher MNWS score before transition than the group in which it decreased after transition. These two factors were significantly related to the difference between the numbers of cigarettes and heat-not-burn tobacco sticks in multiple linear regression analysis. CONCLUSIONS: The mainstream smoke of heat-not-burn tobacco contains harmful substances. There were the possibilities that nicotine dependence and nicotine withdrawal symptoms appear after transition and that the number of heat-not-burn tobacco sticks consumed increases.