RESUMEN
Objective: To investigate the clinicopathologic features, immunophenotype, pathological diagnosis and treatment of malignant mixed tumor (MMT). Methods: Clinical and pathological features including immunohistochemical phenotypes were analyzed in a case of MMT accompanied with eccrine porocarcinoma (EP) involving both hands, diagnosed definitely in January 2018 along with review of relevant literature. Results: A 64-year-old man presented with multiple rash on both hands for 4 years. Three lesions of 0.5 to 2.2 cm were removed for pathological evaluation. The pathological changes on little finger of left and right hands were MMT with EP, whereas that removed from the right ring finger was EP. MMT showed infiltrative growth with vascular wall invasion and consisted of epithelial (glandular or tube differentiation) and mesenchymal components (mucinous and/or cartilage stroma). The endothelial cells showed moderate to severe cytological atypia, nuclear pleomorphism and increased mitotic activity. The glandular component had histological characteristics of syringocarcinoma with moderately atypical chondrocytes but without myoepithelium. EP was composed of basal cells with visible vacuoles in cytoplasm and the presence of tubular and squamous differentiation, along with obvious atypia. Immunohistochemically cavosurface epithelium of glandular differentiation of MMT showed positivity for CK7, EMA and CD117. Myoepithelium showed S-100, CK5/6 and p63 positivity and stromal cells were positive for S-100. Differential diagnoses included metaplastic carcinoma, malignant myoepithelioma and atypical mixed tumor of skin. Conclusions: MMT with EP is extremely rare.The diagnosis of MMT depends on the morphologic features. Immunohistochemical staining is helpful for differential diagnosis. Surgical excision with safety margins is the treatment of choice. Complementary radiotherapy and/or chemotherapy is still controversial. The clinical course of MMT is deemed unpredictable and long-term follow-up is necessary.
Asunto(s)
Porocarcinoma Ecrino/patología , Tumor Mixto Maligno/patología , Neoplasias Cutáneas/patología , Neoplasias de las Glándulas Sudoríparas/patología , Diagnóstico Diferencial , Porocarcinoma Ecrino/química , Epitelio , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Tumor Mixto Maligno/química , Mioepitelioma/química , Mioepitelioma/patología , Proteínas Proto-Oncogénicas c-kit/análisis , Neoplasias Cutáneas/química , Neoplasias de las Glándulas Sudoríparas/químicaRESUMEN
Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The 20, 54, and 87th generations of CEK-adapted IBV-Sczy3 strains were used to infect CEK cells, and then nucleic copy numbers in the samples of supernatant collected at 12, 24, 36, 48, 60, and 72 h were detected. The results showed that the rapid growth period of the virus load of all the 3 generations was approximately 12-36 h post-infection; the peak of the virus load appeared at 36 h post-infection and then decreased gradually in the order of 20th > 54th > 87th for the 3 generations of CEK-adapted strains; the dynamic change curve of the IBV load in the supernatant of primary CEK cells showed a single peak. The results of this study provide a useful reference for CEK-adapted IBV field strains and the production of CEK-attenuated IBV vaccine.
Asunto(s)
Infecciones por Coronaviridae/inmunología , Virus de la Bronquitis Infecciosa/inmunología , Enfermedades de las Aves de Corral/inmunología , Vacunas Atenuadas/inmunología , Vacunas Virales/inmunología , Animales , Embrión de Pollo/inmunología , Embrión de Pollo/virología , Infecciones por Coronaviridae/prevención & control , Infecciones por Coronaviridae/virología , Virus de la Bronquitis Infecciosa/patogenicidad , Riñón/inmunología , Riñón/virología , Enfermedades de las Aves de Corral/prevención & control , Enfermedades de las Aves de Corral/virología , Cultivo Primario de Células , Vacunas Atenuadas/uso terapéutico , Carga Viral/inmunología , Vacunas Virales/uso terapéuticoRESUMEN
The grass carp, Ctenopharyngodon idella (Valenciennes), is one of the most extensively aquacultured freshwater fish in China. However, because of the lack of effective control measures and the high-density culture environment, considerable economic losses are caused by infection of C. idella with the parasitic ciliate, Ichthyophthirius multifiliis. The major histocompatibility (MH) DAB gene belongs to antigen-presented genes in the class II genomic region, which is associated with parasite resistance. To understand the relationship of the DAB gene with I. multifiliis infection in grass carp, the expression profiles of MH II-DAB were studied in tissues using real-time quantitative polymerase chain reaction. The results showed that expression of the MH II-DAB gene was up-regulated in head kidney after I. multifiliis infection, and the expression peak appeared earlier in the study (case) group than in the control group. The obvious up-regulation peak of MH II-DAB gene was found at days 2 and 4 in skin; at 12 h to day 4 in spleen; at 12 h and days 1 and 6 in gill; and at day 10 in blood, whereas the MH II-DAB gene was down-regulated in liver and intestines after I. multifiliis infection. These results have implications for better understanding C. idella resistance to I. multifiliis infection.
Asunto(s)
Carpas/fisiología , Infecciones por Cilióforos/veterinaria , Enfermedades de los Peces/inmunología , Proteínas de Peces/genética , Regulación de la Expresión Génica/inmunología , Animales , Carpas/genética , Carpas/inmunología , Infecciones por Cilióforos/inmunología , Infecciones por Cilióforos/fisiopatología , Enfermedades de los Peces/fisiopatología , Hymenostomatida/fisiología , Complejo Mayor de Histocompatibilidad , Factores de TiempoRESUMEN
To evaluate whether parvovirus B19, a common human pathogen, was also involved in papillary thyroid carcinoma (PTC), 112 paraffin-embedded thyroid specimens of benign nodules, papillary, medullary and follicular carcinomas, and normal controls were examined for B19 DNA and capsid protein by nested PCR, in situ hybridisation (ISH) and immunohistochemistry (IHC). The expression of the nuclear factor-kappaB (NF-kappaB) was investigated by IHC. The results showed B19 DNA commonly exists in human thyroid tissues; however, there were significant differences between PTC group and normal controls, and between PTC and nonneoplastic adjacent tissues (P<0.001). The presence of viral DNA in PTC neoplastic epithelium was confirmed by laser-capture microdissection and sequencing of nested PCR products. B19 capsid protein in PTC group was significantly higher than that of all the control groups and nonneoplastic adjacent tissues (PAsunto(s)
Carcinoma Papilar/virología
, Parvovirus B19 Humano/aislamiento & purificación
, Neoplasias de la Tiroides/virología
, Adulto
, Anciano
, Proteínas de la Cápside/análisis
, ADN Viral/análisis
, Femenino
, Humanos
, Hibridación in Situ
, Masculino
, Persona de Mediana Edad
, FN-kappa B/análisis
, Reacción en Cadena de la Polimerasa
, Estudios Retrospectivos
, Enfermedades de la Tiroides/virología