RESUMEN
OBJECTIVE: MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in carcinogenesis. The present study investigated the role of miR-425-5p in the development and progression of gastric cancer (GC). PATIENTS AND METHODS: The miR-425-5p level in GC tissues and cells was assayed by qRT-PCR. Then, the effects of miR-425-5p expression on the biological behavior of GC cells were investigated. Analysis of target protein expression was determined by Western blotting. Bioinformatic prediction and luciferase assays were employed to identify the predicted miRNA which regulates CYLD. RESULTS: miR-425-5p was found to be up-regulated in GC tissues and cell lines. Knockdown of miR-425-5p in GC cells attenuated migration and invasion of GC cells, whereas overexpression of miR-425-5p promoted cell migration and invasion. The luciferase assay demonstrated that CYLD was a direct target of miR-425-5p. Furthermore, the miR-425-5p level was inversely correlated with levels of CYLD in Western blotting assay. CONCLUSIONS: Our findings indicate that miR-425-5p may contribute to the progression of GC through a mechanism involving CYLD, suggesting that miR-425-5p may have the potential to be a novel important alternative therapeutic target for GC.
Asunto(s)
Enzima Desubiquitinante CYLD/genética , MicroARNs/genética , Neoplasias Gástricas/genética , Carcinogénesis/genética , Movimiento Celular , Técnicas de Silenciamiento del Gen , HumanosRESUMEN
OBJECTIVE: To explore the therapeutic effect of neuronavigation-assisted minimally invasive operation on hypertensive basal ganglia hemorrhage patients with hematoma volume less than 30 mL. PATIENTS AND METHODS: 25 hypertensive basal ganglia hemorrhage patients with hematoma volume varied from 15 to 30 mL were enrolled. 13 patients were recuited to undertook puncture aspiration and catheter drainage under real-time neuronavigation. The operations were carried out under CT imaging guidance. Twelve patients with conservative treatment were recruited as control. RESULTS: Neuronavigation operation group was superior to the conservative treatment group in terms of hematoma clearance time, duration of hospitalization, 6-month Glasgow coma score (GCS) scores and neurological deficiency scores. CONCLUSIONS: Neuronavigation-assisted minimally invasive operation is suitable for low volume hypertensive basal ganglia hemorrhage and improves the prognosis of these patients significantly.