Spatial infiltration and redistribution of light crude oil in heterogeneous water-bearing soil layers under different hydrogeological processes.

Two physical models were used to simulate the infiltration and redistribution process of light crude oil after leakage in a heterogeneous soil layer following water level variation and rainfall. Migration fronts and redistribution characteristics of oil during gravity seepage, water level variation, and rainfall were obtained using charge-coupled device (CCD) camera shooting and cyan-magenta-yellow‒black (CMYK)-based gray analysis, which were employed efficiently and at a low cost. Then, the influencing factors and migration mechanisms were examined. Finally, the soil water and oil contents were measured to verify the simulation results. The results are as follows: (1) the geologic lens and fine-coarse interface can intercept oil, resulting in a local highly contaminated area. (2) The crude oil infiltration path and velocity varied greatly with the different soil types and initial water contents. Within a certain range, the higher the initial water content is, the higher the lateral and vertical infiltration speeds. (3) The oil redistribution process was dominated by vertical infiltration under the condition of water level variation or rainfall, but oil-water displacement and the capillary pressure caused some oil to move horizontally near the geologic lens and fine-coarse interface. (4) Water level variation resulted in a synchronous rise or fall of the oil accumulation area, but rainfall caused it to move up. (5) Water level variation and rainfall imposed a certain influence on the periodic accumulation and release of crude oil in heterogeneous soil, especially in the presence of geologic lenses and lithologic interfaces.

Deep learning assisted plenoptic wavefront sensor for direct wavefront detection.

Traditional plenoptic wavefront sensors (PWFS) suffer from the obvious step change of the slope response, leading to poor wavefront detection performance. In order to solve this problem, in this paper, a deep learning model is proposed to restore phase maps directly from slope measurements of PWFS. Numerical simulations are employed to demonstrate our approach, and the statistical residual wavefront root mean square error (RMSE) of our method is 0.0810 ± 0.0258λ, which is much superior to those of modal algorithm (0.2511 ± 0.0587λ) and zonal approach (0.3584 ± 0.0487λ). The internal driving force of PWFS-ResUnet is investigated, and the slope response differences between sub-apertures and directions are considered as a probably key role to help our model to accurately restore the phase map. Additionally, the robustness of our model to turbulence strength and signal-to-noise ratio (SNR) level is also tested. The proposed method provides a new direction to solve the nonlinear problem of traditional PWFS.

Direct wavefront sensing with a plenoptic sensor based on deep learning.

Traditional plenoptic wavefront sensors (PWS) suffer from the obvious step change of the slope response which leads to the poor performance of phase retrieval. In this paper, a neural network model combining the transformer architecture with the U-Net model is utilized to restore wavefront directly from the plenoptic image of PWS. The simulation results show that the averaged root mean square error (RMSE) of residual wavefront is less than 1/14λ (Marechal criterion), proving the proposed method successfully breaks through the non-linear problem existed in PWS wavefront sensing. In addition, our model performs better than the recently developed deep learning models and traditional modal approach. Furthermore, the robustness of our model to turbulence strength and signal level is also tested, proving the good generalizability of our model. To the best of our knowledge, it is the first time to perform direct wavefront detection with a deep-learning-based method in PWS-based applications and achieve the state-of-the-art performance.

Identification of a primary antigenic target of epitope spreading in endemic pemphigus foliaceus.

Epitope spreading is an important mechanism for the development of autoantibodies (autoAbs) in autoimmune diseases. The study of epitope spreading in human autoimmune diseases is limited due to the major challenge of identifying the initial/primary target epitopes on autoantigens in autoimmune diseases. We have been studying the development of autoAbs in an endemic human autoimmune disease, Brazilian pemphigus foliaceus (or Fogo Selvagem (FS)). Our previous findings demonstrated that patients before (i.e. preclinical) and at the onset of FS have antibody (Ab) responses against other keratinocyte adhesion molecules in addition to the main target autoantigen of FS, desmoglein 1 (Dsg1), and anti-Dsg1 monoclonal Abs (mAbs) cross-reacted with an environmental antigen LJM11, a sand fly saliva protein. Since sand fly is prevalent in FS endemic regions, individuals in these regions could develop Abs against LJM11. The anti-LJM11 Abs could recognize different epitopes on LJM11, including an epitope that shares the structure similarity with an epitope on Dsg1 autoantigen. Thus, Ab response against this epitope on LJM11 could be the initial autoAb response detected in individuals in FS endemic regions, including those who eventually developed FS. Accordingly, this LJM11 and Dsg1 cross-reactive epitope on Dsg1 could be the primary target of the autoimmune response in FS. This investigation aimed to determine whether the autoAb responses against keratinocyte adhesion molecules are linked and originate from the immune response to LJM11. The anti-Dsg1 mAbs from preclinical FS and FS individuals were employed to determine their specificity or cross-reactivity to LJM11 and keratinocyte adhesion molecules. The cross-reactive epitopes on autoantigens were mapped. Our results indicate that all tested mAbs cross-reacted with LJM11 and keratinocyte adhesion molecules, and we identified an epitope on these keratinocyte adhesion molecules which is mimicked by LJM11. Thus, the cross-reactivity could be the mechanism by which the immune response against an environmental antigen triggers the initial autoAb responses. Epitope spreading leads to the pathogenic autoAb development and ensuing FS among genetically susceptible individuals.

Methylphenidate and Morphine Combination Therapy in a Rat Model of Chronic Pain.

BACKGROUND: The incremental dose of opioids used in chronic pain management often leads to a reduced opioid analgesic effect, opioid misuse, and addiction. Central dopamine (DA) dysfunction contributes to the chronicity of pain and a decreased opioid analgesic effect. Methylphenidate (MPH/Ritalin) enhances central DA function by inhibiting DA reuptake. In this study, we used a rat model of chronic pain to examine whether combination of MPH with morphine (MOR) would improve the MOR analgesic effect under a chronic pain condition. METHODS: Tibiotarsal joint Complete Freund's Adjuvant (CFA) injection in rats was utilized to induce chronic nociception. The analgesic effect of low-dose MPH (0.25 mg/kg), low-dose MOR (2.5 mg/kg), and their combination was examined in CFA rats. Nociceptive behavior was assessed by von Frey test. Conditioned place preference (CPP) and open field tests (OFTs) were used to examine the rewarding behavior and locomotor activity in rats, respectively. RESULTS: Our findings are as follows: (1) in CFA rats with chronic pain, 2.5 mg/kg of MOR had less analgesic effect than 10 mg/kg of MOR at 28 days after injury (95% confidence intervals [CIs] for difference of means of von Frey threshold in gram: -11.9 [-6.5 to -17.3]); (2) in the 1-hour time window of 30-90 minutes after injection, the combination of MPH (0.25 mg/kg) with MOR (2.5 mg/kg) increased synergistically and prolonged the analgesic effect in CFA rats as compared with MPH or MOR alone (P = .01 for MPH by MOR interaction, and 95% CIs for difference of means of von Frey threshold in gram: 3.3 [1.37-6.12] for the combination versus MPH and 3.2 [1.35-5.74] for the combination versus MOR); (3) at the low dose (0.25 mg/kg), MPH did not increase locomotor activity (MOR + MPH versus MOR, P = .13) nor significantly enhanced MOR reward behavior (MOR + MPH versus MOR, P = .63) in CFA rats. CONCLUSIONS: Our data suggest that a combination therapy using low-dose MPH and MOR may produce a MOR-sparing effect in chronic pain management.

Macrodiversity Reception with Distributed Hard-Decision Receivers for Maritime Wireless Sensor Networks.

Maritime wireless sensor networks are considered to be the primary means of monitoring methods in the marine environment. The transmission between sensor node and sink node in maritime wireless sensor networks is usually unreliable due to the harsh propagation environment. To extend the transmission range or to enhance the transmission reliability between sensor nodes and sink node, we propose a macrodiversity reception scheme in the sink node equipped with distributed multiple hard-decision receivers. Multiple receivers are divided into several clusters and placed at different locations to receive different signal copies suffering from different fadings. Furthermore, a cascaded combining strategy based on hard-decision information is used to reduce the overall complexity of receiving side. The experimental results in the ocean scenarios show that the macrodiversity reception scheme with two antenna clusters has a transmission gain of 3-4 dB compared with the single antenna reception when the package loss rate is 10 - 2 . The study casts a new method for reliable transmission in maritime wireless sensor networks using commercial transceivers which can only output hard-decision results.

Simulation of Nanopore Sequencing Signals Based on BiGRU.

Oxford Nanopore sequencing is an important sequencing technology, which reads the nucleotide sequence by detecting the electrical current signal changes when DNA molecule is forced to pass through a biological nanopore. The research on signal simulation of nanopore sequencing is highly desirable for method developments of nanopore sequencing applications. To improve the simulation accuracy, we propose a novel signal simulation method based on Bi-directional Gated Recurrent Units (BiGRU). In this method, the signal processing model based on BiGRU is built to replace the traditional low-pass filter to post-process the ground-truth signal calculated by the input nucleotide sequence and nanopore sequencing pore model. Gaussian noise is then added to the filtered signal to generate the final simulated signal. This method can accurately model the relation between ground-truth signal and real-world sequencing signal through experimental sequencing data. The simulation results reveal that the proposed method utilizing the powerful learning ability of the neural network can generate the simulated signal that is closer to the real-world sequencing signal in the time and frequency domains than the existing simulation method.

Walnut antigens can trigger autoantibody development in patients with pemphigus vulgaris through a "hit-and-run" mechanism.

BACKGROUND: Environmental factors, as well as genetic predisposition, are known to be critical for the development of autoimmunity. However, the environmental agents that trigger autoimmune responses have remained elusive. One possible explanation is the "hit-and-run" mechanism in which the inciting antigens that initiate autoimmune responses are not present at the time of overt autoimmune disease. OBJECTIVE: After our previous findings that some allergens can incite autoimmune responses, we investigated the potential role of environmental allergens in triggering autoantibody development in patients with an autoimmune skin disease, pemphigus vulgaris (PV). METHODS: Revertant/germline mAbs (with mutations on variable regions of heavy and light chains reverted to germline forms) of 8 anti-desmoglein (Dsg) 3 pathogenic mAbs from patients with PV were tested for reactivity against a panel of possible allergens, including insects, pollens, epithelia, fungi, and food antigens. RESULTS: All the PV germline mAbs were reactive to antigens from walnut, including the well-known allergen Jug r 2 and an uncharacterized 85-kDa protein component. Sera from patients with PV contained significantly greater levels of anti-Dsg3 autoantibodies than walnut-specific antibodies, suggesting that the autoreactive B-cell response in patients with PV might be initially triggered by walnut antigens but is subsequently driven by Dsg3. CONCLUSION: Our findings suggest that walnut antigens/allergens can initiate autoantibody development in patients with PV through a "hit-and-run" mechanism. The revertant/germline mAb approach might provide a paradigm for the etiological study of other allergic and autoimmune diseases.

Persistent Nociception Facilitates the Extinction of Morphine-Induced Conditioned Place Preference.

BACKGROUND: As opioid abuse and addiction have developed into a major national health crisis, prescription of opioids for pain management has become more controversial. However, opioids do help some patients by providing pain relief and improving the quality of life. To better understand the addictive properties of opioids under chronic pain conditions, we used a conditioned place preference (CPP) paradigm to examine the rewarding properties of morphine in rats with persistent nociception. METHODS: Spared nerve injury (SNI) model was used to induce persistent nociception in rats. Nociceptive behavior was assessed by von Frey test. CPP test was used to examine the rewarding properties of morphine. RESULTS: Our findings are as follows: (1) SNI rats did not show a difference compared with sham rats in magnitude of morphine-induced CPP 1 day after last morphine injection (2-way analysis of variance; for SNI versus sham, F[1,42] = 0.014, P = .91; and 95% confidence intervals for difference of means, -5.9 [-58 to 46], 0.76 [-51 to 53], and 0.90 [-51 to 53] for 2.5, 5, and 10 mg/kg, respectively); (2) increasing morphine dosage (2.5, 5, and 10 mg/kg) did not further increase the magnitude of CPP in both sham and SNI rats (for dosage: F[2,42] = 0.94, P = .40); and (3) morphine-induced CPP persisted in sham rats but extinguished in SNI rats when tested at 8 days after last morphine injection (for sham versus SNI: Bonferroni correction, P < .006 for both 5 and 10 mg/kg doses; and 95% confidence intervals for difference of means, 80.3 [19.7-141] and 87.0 [26.3-148] for 5 and 10 mg/kg, respectively). CONCLUSIONS: Our data provide new evidence supporting the notion that the brain's reward circuitry changes in the context of persistent pain. This observational study suggests that future investigation into the neurobiology of opioid reward requires consideration of the circumstances in which opioid analgesics are administered.

Improving Retinal Image Quality Using Registration with an SIFT Algorithm in Quasi-Confocal Line Scanning Ophthalmoscope.

When high-magnification images are taken with a quasi-confocal line scanning ophthalmoscope (LSO), the quality of images always suffers from Gaussian noise, and the signal to noise ratio (SNR) is very low for a safer laser illumination. In addition, motions of the retina severely affect the stabilization of the real-time video resulting in significant distortions or warped images. We describe a scale-invariant feature transform (SIFT) algorithm to automatically abstract corner points with subpixel resolution and match these points in sequential images using an affine transformation. Once n images are aligned and averaged, the noise level drops by a factor of [Formula: see text] and the image quality is improved. The improvement of image quality is independent of the acquisition method as long as the image is not warped, particularly severely during confocal scanning. Consequently, even better results can be expected by implementing this image processing technique on higher resolution images.

Optical Design of Adaptive Optics Confocal Scanning Laser Ophthalmoscope with Two Deformable Mirrors.

We describe the optical design of a confocal scanning laser ophthalmoscope with two deformable mirrors. Spherical mirrors are used for pupil relay. Defocus aberration of the human eye is corrected by a Badal focusing structure and astigmatism aberration is corrected by a deformable mirror. The main optical system achieves a diffraction-limited performance through the entire scanning field (6 mm pupil, 3 degrees on pupil plane). The performance of the optical system, with correction of defocus and astigmatism, is also evaluated.

Multi-Source Cooperative Data Collection with a Mobile Sink for the Wireless Sensor Network.

The multi-source cooperation integrating distributed low-density parity-check codes is investigated to jointly collect data from multiple sensor nodes to the mobile sink in the wireless sensor network. The one-round and two-round cooperative data collection schemes are proposed according to the moving trajectories of the sink node. Specifically, two sparse cooperation models are firstly formed based on geographical locations of sensor source nodes, the impairment of inter-node wireless channels and moving trajectories of the mobile sink. Then, distributed low-density parity-check codes are devised to match the directed graphs and cooperation matrices related with the cooperation models. In the proposed schemes, each source node has quite low complexity attributed to the sparse cooperation and the distributed processing. Simulation results reveal that the proposed cooperative data collection schemes obtain significant bit error rate performance and the two-round cooperation exhibits better performance compared with the one-round scheme. The performance can be further improved when more source nodes participate in the sparse cooperation. For the two-round data collection schemes, the performance is evaluated for the wireless sensor networks with different moving trajectories and the variant data sizes.

Guasha improves the rating of perceived exertion scale score and reduces heart rate variability in male weightlifters: a randomized controlled trial.

OBJECTIVE: To evaluate the effects of Guasha therapy on the rating of perceived exertion (RPE) scale score, and heart rate variability (HRV). METHODS: A randomized controlled trial of Guasha (skin scraping) was compared with a sham scraping group and control group. Sixteen sessions within an 8-week period were completed. Sixty-five male weightlifters who had undergone normal weightlifting training for a mean of 5 years before study commencement were recruited. The RPE scale score of "snatch", "clean and jerk" maneuvers (85% of one-repetition maximum), and HRV were measured before and after the intervention. RESULTS: The RPE scale score for snatch, clean and jerk were reduced significantly after intervention in the Guasha group and sham group. However, there was a significant difference in the low frequency (LF) domain and LF/high frequency (HF) ratio (P < 0.05): the LF domain decreased, and the LF/HF ratio decreased. CONCLUSION: Guasha could be used to reduce the RPE scale score, and increase the response to HRV. Guasha could be considered as an alternative to some types of recovery from sports training.

Design of a Compact, Bimorph Deformable Mirror-Based Adaptive Optics Scanning Laser Ophthalmoscope.

We have designed, constructed and tested an adaptive optics scanning laser ophthalmoscope (AOSLO) using a bimorph mirror. The simulated AOSLO system achieves diffraction-limited criterion through all the raster scanning fields (6.4 mm pupil, 3° × 3° on pupil). The bimorph mirror-based AOSLO corrected ocular aberrations in model eyes to less than 0.1 µm RMS wavefront error with a closed-loop bandwidth of a few Hz. Facilitated with a bimorph mirror at a stroke of ±15 µm with 35 elements and an aperture of 20 mm, the new AOSLO system has a size only half that of the first-generation AOSLO system. The significant increase in stroke allows for large ocular aberrations such as defocus in the range of ±600° and astigmatism in the range of ±200°, thereby fully exploiting the AO correcting capabilities for diseased human eyes in the future.

Effect of acupuncture treatment on smoking cessation in smokers from Hong Kong.

OBJECTIVE: To analyze the characteristics of smokers treated with acupuncture for smoking cessation in Hong Kong. METHODS: A total of 2051 subjects were recruited in a clinical pilot research project "acupuncture for smoking cessation", which was conducted jointly by Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences and Hong Kong Pok Oi Hospital from January of 2011 to December of 2013. The characteristics of study subjects, including baseline information, smoking background, intention to quit and influencing factors were analyzed. RESULTS: The majority of subjects treated with acupuncture for smoking cessation in Hong Kong was male (66.7% ), but the proportion of female smokers in this study (33.3%) was higher than that of female smokers in Hong Kong population (13.8%, P < 0.05). Subjects were at the mean age of 43.83 years old, of which the percentage of females aged 31-40 years was the highest (38.8% , P < 0.05). The mean duration of smoking was 25.49 years. The number of cigarettes smoked per day was 17.57 cigarettes. Fagerstrom Test for Nicotine Dependence (FTND) was 5.29 points. Most of the subjects had attempted quitting smoking (81.42% ). The confidence index (7.44 points) and the readiness to quit smoking (8.13 points) were high. Subjects quitting smoking were mostly due to health cause (81.91%). The majority of subjects were at the level of middle school (61.63%). The higher the educational level was, the lower the tobacco dependence was and the higher the confidence in successfully quitting smoking was. 50.27% of subjects chose acupuncture for smoking cessation mainly through friends, television and network publicity. Subjects who had received acupuncture had the highest confidence index, while those who wanted to try a new method had the lower confidence index. smoking cessation mainly through friends, television and network publicity. Subjects who had received acupuncture had the highest confidence index, while those who wanted to try a new method had the lower confidence index. CONCLUSION: Acupuncture for smoking cessation was more popular in female smokers, especially those aged 31-40 years. The effectiveness of acupuncture-smoking cessation was most significant in the smokers over 60.

[Absorption characteristics of alkaloids in Fuzheng Xiaozheng Fang by rat everted intestinal sac models].

Everted intestinal sac models were used to investigate the intestinal absorption of the 4 alkaloids(berberine, palmatine, coptisine, and epiberberine) in Fuzheng Xiaozheng Fang(FZ) at different intestine segments. The absorption parameters of each component were calculated; SPSS 20.0 software was used to analyze the data and evaluate the absorption characteristics at different intestinal segments. The results showed that all the four active ingredients conformed to zero-order absorption rate. There was significant difference in absorption rate constant (Ka) between the four ingredients at low dose and medium and high dose groups(P<0.05), but there was no significant difference in Ka between medium dose and high dose. The absorption mechanization of four ingredients presented two absorption manners： positive diffusion and passive absorption. The absorptive amount of 4 alkaloids in ileum was slightly greater than that of jejunum, but no significant differences were observed, which indicated that these four alkaloids had no specific absorption windows in intestinal segment.

Extension of the modal wave-front reconstruction algorithm to non-uniform illumination.

Attempts are made to eliminate the effects of non-uniform illumination on the precision of wave-front measurement. To achieve this, the relationship between the wave-front slope at a single sub-aperture and the distributions of the phase and light intensity of the wave-front were first analyzed to obtain the relevant theoretical formulae. Then, based on the principle of modal wave-front reconstruction, the influence of the light intensity distribution on the wave-front slope is introduced into the calculation of the reconstruction matrix. Experiments were conducted to prove that the corrected modal wave-front reconstruction algorithm improved the accuracy of wave-front reconstruction. Moreover, the correction is conducive to high-precision wave-front measurement using a Hartmann wave-front sensor in the presence of non-uniform illumination.

MRI reveals differential effects of amphetamine exposure on neuroglia in vivo.

How amphetamine affects the neuroglia in living brains is not well understood. In an effort to elucidate this effect, we investigated neuroglia in response to amphetamine exposure using antisense (AS) or sense (S) phosphorothioate-modified oligodeoxynucleotide (sODN) sequences that correspond to glial fibrillary acidic protein (GFAP) mRNA (AS-gfap or S-gfap, respectively) expression. The control is a random-sequence sODN (Ran). Using cyanine 5.5-superparamagnetic iron oxide nanoparticle (Cy5.5-SPION) labeling and fluorescent microscopy, we demonstrated that living neural progenitor cells (PC-12.1), as well as the cells in fresh brain slices and intact brains of male C57BL6 mice, exhibited universal uptake of all of the sODNs but rapidly excluded all sODN-Ran and most S-gfap. Moreover, transmission electron microscopy revealed electron-dense nanoparticles only in the neuroglia of normal or transgenic mice [B6;DBA-Tg(Fos-tTA, Fos-EGFP*)1MmayTg(tetO-lacZ,tTA*)1Mmay/J] that had been administered AS-gfap or Cy5.5-SPION-gfap. Subtraction R2* maps from mice with acute and chronic amphetamine exposure demonstrated, validated by postmortem immunohistochemistry, a reduction in striatal neuroglia, with gliogenesis in the subventricular zone and the somatosensory cortex in vivo. The sensitivity of our unique gene transcript targeted MRI was illustrated by a positive linear correlation (r(2)=1.0) between in vivo MRI signal changes and GFAP mRNA copy numbers determined by ex vivo quantitative RT-PCR. The study provides direct evidence for targeting neuroglia by antisense DNA-based SPION-gfap that enables in vivo MRI of inaccessible tissue with PCR sensitivity. The results enable us to conclude that amphetamine induces toxicity to neuroglia in vivo, which may cause remodeling or reconnectivity of neuroglia.

Effect of scraping therapy on weightlifting ability.

OBJECTIVE: To verify the effects of scraping therapy on the weightlifting ability by measuring the subjective sensation, and changes of biomarkers. METHODS: Five students, who have been trained for 3 years in a sport school in China were participated in this study. A course of scraping therapy was applied to intervene during the normal 7-week of weightlifting training programme. The ability of weightlifting, the scale of rating perceived exertion and serum biochemical markers were measured before and after the intervention. RESULTS: Scraping therapy caused a significant increase in weightlifting ability (P < 0.05). The level of rating perceived exertion remained stable with the increase in the training volume. Immuno-globulin A was significantly increased (P < 0.05), and creatine kinase and blood urea nitrogen were significantly decreased (P < 0.05). No significant changes were observed in white blood cell, neutrophil, and testosterone. CONCLUSION: Scraping therapy may facilitate weightlifting ability mainly by decreasing weight sensation and improving serum biochemical parameters.

Use of laser speckle contrast imaging to reveal changes in temperature and blood perfusion in the skin of healthy subjects after administration of heated moxa sticks and daiwenjiu ointment.

OBJECTIVE: To investigate the influence of heated moxa sticks ("moxibustion") and Daiwenjiu ointment (DO) on changes in temperature and blood perfusion volume on the skin of the backs of healthy subjects. METHODS: DO was spread on the left side of the body, and the right side of the body was treated with a heated moxa stick. Images denoting blood perfusion and body temperature were collected 7-8 cm lateral to the spinous process of the sixth thoracic vertebra using laser speckle contrast imaging (LSCI). Data obtained from eight-frame images were analyzed and used to calculate the mean blood perfusion volume. Simultaneously, blood-perfusion images were collected from the body surface and used to compare the change in blood flow on the body surface and the actual position of imaging. RESULTS: After moxibustion, a rapid increase in blood perfusion volume and body temperature was noted in the local skin surface. The maximum blood perfusion volume and body temperature was noted at 20 min (P < 0.05). At 80-110 min after the spreading of DO, a gradual increase was noted in blood perfusion volume (P < 0.05) and body temperature (P < 0.05) compared with the baseline level. The maximum blood perfusion volume was at 110 min. CONCLUSION: Using LSCI, these data revealed a rapid and sharp increase in blood perfusion volume and body temperature after treatment with moxibustion, but the respective changes seen in the DO group were gradual and moderate.