RESUMEN
BACKGROUND: To provide information for umbilical cord blood (UCB) banks to adopt optimal collection strategies and to make UCB banks operate efficiently, we investigated the reasons for exclusion of UCB units in a 3-year recruitment period. STUDY DESIGN AND METHODS: We analyzed records of the reasons for exclusion of the potential UCB donation from 2004 to 2006 in the Tzu-Chi Cord Blood Bank and compared the results over 3 years. We grouped these reasons for exclusion into five phases, before collection, during delivery, before processing, during processing, and after freezing according to the time sequence and analyzed the reasons at each phase. RESULTS: Between 2004 and 2006, there were 10,685 deliveries with the intention of UCB donation. In total, 41.2% of the UCB units were considered eligible for transplantation. The exclusion rates were 93.1, 48.4, and 54.1% in 2004, 2005, and 2006, respectively. We excluded 612 donations from women before their child birth, 133 UCB units during delivery, 80 units before processing, 5010 units during processing, and 421 units after freezing. There were 24 UCB units with unknown reasons of ineligibility. Low UCB weight and low cell count were the first two leading causes of exclusion (48.6 and 30.9%). The prevalence of artificial errors, holiday or transportation problem, low weight, and infant problems decreased year after year. CONCLUSION: The exclusion rate was high at the beginning of our study as in previous studies. Understanding the reasons for UCB exclusion may help to improve the efficiency of UCB banking programs in the future.
Asunto(s)
Almacenamiento de Sangre/métodos , Donantes de Sangre , Sangre Fetal , Selección de Paciente , Bancos de Sangre/organización & administración , Bancos de Sangre/estadística & datos numéricos , Donantes de Sangre/estadística & datos numéricos , Eficiencia Organizacional , Femenino , Humanos , Recién Nacido , Recien Nacido Prematuro/sangre , Meconio/fisiología , Embarazo , Complicaciones del Embarazo/sangre , Complicaciones del Embarazo/epidemiología , Sector PúblicoRESUMEN
One nucleotide substitution in codon 131 of HLA-C*03:04:01:01 results in a novel allele, HLA-C*03:04:89.
Asunto(s)
Antígenos HLA-C , Humanos , Secuencia de Bases , Antígenos HLA-C/genética , Alelos , Prueba de Histocompatibilidad , Codón , Taiwán , Análisis de Secuencia de ADN/métodosRESUMEN
One nucleotide substitution in codon 217 of HLA-DRB1*09:01:02:01 results in a novel allele HLA-DRB1*09:01:15.
Asunto(s)
Cadenas HLA-DRB1 , Humanos , Cadenas HLA-DRB1/genética , Secuencia de Bases , Alelos , Exones/genética , Codón , Análisis de Secuencia de ADN , Taiwán , Prueba de HistocompatibilidadRESUMEN
A nucleotide mutation in codon 171 of HLA-A*11:01:01:01 results in a novel allele HLA-A*11:127N.
Asunto(s)
Alelos , Codón , Exones , Antígeno HLA-A11 , Prueba de Histocompatibilidad , Humanos , Taiwán , Antígeno HLA-A11/genética , Exones/genética , Prueba de Histocompatibilidad/métodos , Secuencia de Bases , Análisis de Secuencia de ADN/métodos , Pueblo Asiatico/genéticaRESUMEN
One nucleotide substitution in codon 81 of HLA-B*40:01:01 results in a novel allele, HLA-B*40:400.
Asunto(s)
Alelos , Exones , Antígeno HLA-B40 , Haplotipos , Prueba de Histocompatibilidad , Humanos , Taiwán , Antígeno HLA-B40/genética , Secuencia de Bases , Codón , Análisis de Secuencia de ADN/métodos , Pueblo Asiatico/genética , Polimorfismo de Nucleótido Simple , Alineación de Secuencia , Antígenos HLA-B/genéticaRESUMEN
One nucleotide substitution in codon 264 of HLA-C*07:02:01:01 results in a novel allele, HLA-C*07:359.
Asunto(s)
Antígenos HLA-C , Polimorfismo de Nucleótido Simple , Humanos , Secuencia de Bases , Antígenos HLA-C/genética , Alelos , Sustitución de Aminoácidos , Exones/genética , Análisis de Secuencia de ADN/métodos , Prueba de Histocompatibilidad , Donantes de Tejidos , TaiwánRESUMEN
Two nucleotide substitutions in exon 3 of HLA-A*02:07:01:01 result in the novel allele, HLA-A*02:840.
Asunto(s)
Pueblo Asiatico , Antígenos HLA-A , Nucleótidos , Humanos , Alelos , Pueblo Asiatico/genética , Exones/genética , Antígenos HLA-A/genética , TaiwánRESUMEN
Nucleotide substitutions in codons -1 and 84 of HLA-B*40:01:01 result in a novel allele, HLA-B*40:01:35.
Asunto(s)
Pueblo Asiatico , Genes MHC Clase I , Humanos , Alelos , Pueblo Asiatico/genética , Antígenos HLA-B/genética , NucleótidosRESUMEN
Nucleotide substitution in codon 129 of HLA-B*15:01:01:01 results in a novel allele, HLA-B*15:01:17.
Asunto(s)
Genes MHC Clase I , Antígenos HLA-B , Humanos , Alelos , Antígenos HLA-B/genética , Codón , Pueblo Asiatico/genética , Taiwán , Análisis de Secuencia de ADN/métodos , Prueba de Histocompatibilidad/métodosRESUMEN
One nucleotide substitution in codon 214 of HLA-C*03:04:01:01 results in a novel allele, HLA-C*03:649.
Asunto(s)
Antígenos HLA-C , Humanos , Secuencia de Bases , Antígenos HLA-C/genética , Alelos , Exones/genética , Codón , Taiwán , Análisis de Secuencia de ADN , Prueba de HistocompatibilidadRESUMEN
One nucleotide substitution in codon 130 of HLA-DQB1*03:03:02:01 results in a novel allele HLA-DQB1*03:96.
Asunto(s)
Alelos , Codón , Exones , Cadenas beta de HLA-DQ , Prueba de Histocompatibilidad , Humanos , Cadenas beta de HLA-DQ/genética , Taiwán , Secuencia de Bases , Pueblo Asiatico/genética , Análisis de Secuencia de ADN/métodos , Polimorfismo de Nucleótido SimpleRESUMEN
Two nucleotide substitutions in codon 152 of HLA-C*08:01:01:01 result in a novel allele HLA-C*08:66.
Asunto(s)
Exones , Antígenos HLA-C , Prueba de Histocompatibilidad , Humanos , Alelos , Secuencia de Bases , Codón , Prueba de Histocompatibilidad/métodos , Antígenos HLA-C/genética , Alineación de Secuencia , Análisis de Secuencia de ADN/métodos , TaiwánRESUMEN
A nucleotide deletion in the residue 371 of HLA-A*11:01:01:01 results in a novel allele HLA-A*11:466N.
Asunto(s)
Exones , Antígeno HLA-A11 , Prueba de Histocompatibilidad , Humanos , Alelos , Secuencia de Bases , Codón , Antígeno HLA-A11/genética , Alineación de Secuencia , Análisis de Secuencia de ADN , Eliminación de Secuencia , TaiwánRESUMEN
Nucleotide substitution in codon 238 of HLA-C*12:02:02:01 results in a novel allele, HLA-C*12:02:53.
Asunto(s)
Alelos , Secuencia de Bases , Codón , Exones , Antígenos HLA-C , Prueba de Histocompatibilidad , Humanos , Sustitución de Aminoácidos , Pueblo Asiatico/genética , Antígenos HLA-C/genética , Polimorfismo de Nucleótido Simple , Alineación de Secuencia , Análisis de Secuencia de ADN , TaiwánRESUMEN
Objectives: It is thought that Taiwanese indigenous people were the "first people" to populate Taiwan (Formosa) having been there for over 5000 years, preceding the Dutch colonization (from 1624 to 1662) and Spanish colonization (from 1626 to 1642). Taiwan's indigenes, represented by Austronesian language speakers, currently constitute approximately 2% of the total population in Taiwan. It is unknown whether they evolved from Taiwan's Paleolithic or Neolithic cultures, arrived during or after the Neolithic period from China or Southeast Asia or both. HLA studies on the Taiwanese indigenous population have found several intriguing genetic information showing one or two relatively frequently observed alleles and a small number of relatively less frequently observed ones. We report here a relatively frequently observed HLA-C*07:359 allele in the Taiwanese indigenous population, its linkage with HLA-B*39:01, and its probable associated HLA haplotype in two Taiwanese indigenous families. HLA-C*07:359 is a rarely observed allele in the HLA-C locus in the world populations. The objective of this study is to report the allele HLA-C*07:359 that is more frequently found in the Taiwanese population, especially in the Taiwanese indigenous people, to demonstrate that it has a close linkage with HLA-B*39:01 allele in the HLA-B locus and to show the plausible deduced HLA-A-C-B-DRB1-DQB1 haplotypes in association with HLA-C*07:359 in two families of Taiwanese indigenous unrelated individuals. Materials and Methods: The samples were peripheral whole blood, with dipotassium ethylenediaminetetraacetic acid and/or acid citrate dextrose anticoagulation additives. The sequence-based typing method was employed to confirm the low incidence of the allele of HLA-C*07:359 observed in Taiwanese. Polymerase chain reaction was carried out to amplify exons 2, 3, and 4 of the HLA-A,-B,-C,-DRB1 and-DQB1 loci with group-specific primer sets. Amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions according to the manufacturer's protocol. Results: C*07:359 is an uncommon allele in the HLA-C locus in the world general population, according to our literature review. However, in this study, it is observed in the general Taiwanese population (frequency 0.41%), especially in the Taiwanese indigenous people at a frequency of 0.23%. In addition, we deduced two probable HLA haplotypes in association with C*07:359 in two indigenous families: A*24:02-C*07:359-B*39:01-DRB1*04:36 and A*24:02-C*07:359-B*39:01-DRB1*04:04. Conclusion: The two deduced HLA haplotypes associated with the uncommon C*07:359 allele that we report here are valuable for HLA tissue typing laboratories for reference purposes and for stem cell transplantation donor search coordinators to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries for patients bearing the uncommon HLA allele. Since C*07:359 was found mostly in the Taiwanese indigenous population, we think the allele and its haplotypes we report here are important in population and anthropological studies.
RESUMEN
Hematopoietic stem cell (HSC) transplantation, using either bone marrow (BM) or peripheral blood stem cells (PBSC), is a well-established therapy for various hematologic and non-hematologic diseases. However, the long-term health outcomes after HSC donation remain a major concern for several potential donors. Thus, we aimed to conduct a matched cohort study of 5003 unrelated donors (1099 BM and 3904 PBSC) and randomly selected 50,030 matched controls based on age, sex, and resident area from the donor registry between 1998 and 2018. The medical insurance claims of all the participants were retrieved from the Taiwan National Health and Welfare Data Science Center after de-identification. Our findings revealed no differences in the incidence of cancer, death, and catastrophic diseases between HSC donors and matched healthy participants during long-term follow-up. Kaplan-Meier curves depicting the cumulative incidence of cancer and overall mortality throughout the follow-up period also demonstrated similar outcomes between donors and non-donors. In conclusion, our results indicate that HSC donation, whether through BM or PBSC, is safe and not associated with an increased risk of cancer, death, or catastrophic diseases. These findings provide valuable information for counseling potential HSC donors and for long-term management of HSC donor health.
Asunto(s)
Trasplante de Células Madre Hematopoyéticas , Neoplasias , Humanos , Neoplasias/terapia , Masculino , Femenino , Estudios de Seguimiento , Adulto , Trasplante de Células Madre Hematopoyéticas/métodos , Persona de Mediana Edad , Estudios de Cohortes , Enfermedad Catastrófica , Taiwán/epidemiología , Donantes de TejidosRESUMEN
One nucleotide substitution in codon 317 of HLA-B*57:01:01:01 results in the novel allele, HLA-B*57:164.
Asunto(s)
Antígenos HLA-B , Humanos , Secuencia de Bases , Alelos , Prueba de Histocompatibilidad , Antígenos HLA-B/genética , Taiwán , Análisis de Secuencia de ADNRESUMEN
One nucleotide substitution in codon 227 of HLA-C*03:04:01:01 results in a novel allele, HLA-C*03:04:20.
Asunto(s)
Antígenos HLA-C , Humanos , Secuencia de Bases , Antígenos HLA-C/genética , Alelos , Prueba de Histocompatibilidad , Análisis de Secuencia de ADN , TaiwánRESUMEN
One nucleotide substitution in codon 140 of HLA-DRB1*04:05:01:01 results in a novel allele, HLA-DRB1*04:358.
Asunto(s)
Secuencia de Bases , Humanos , Alelos , Exones/genética , Codón , Cadenas HLA-DRB1/genética , Análisis de Secuencia de ADN , Taiwán , Prueba de HistocompatibilidadRESUMEN
One nucleotide substitution in codon 25 of HLA-DQB1*03:01:01:01 results in a novel allele HLA-DQB1*03:151.