m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma.

BACKGROUND: Lung adenocarcinoma (LUAD) is a common type of lung cancer with a high risk of metastasis, but the exact molecular mechanisms of metastasis are not yet understood. METHODS: This study acquired single-cell transcriptomics profiling of 11 distal normal lung tissues, 11 primary LUAD tissues, and 4 metastatic LUAD tissues from the GSE131907 dataset. The lung multicellular ecosystems were characterized at a single-cell resolution, and the potential mechanisms underlying angiogenesis and metastasis of LUAD were explored. RESULTS: We constructed a global single-cell landscape of 93,610 cells from primary and metastatic LUAD and found that IGF2BP2 was specifically expressed both in a LUAD cell subpopulation (termed as LUAD_IGF2BP2), and an endothelial cell subpopulation (termed as En_IGF2BP2). The LUAD_IGF2BP2 subpopulation progressively formed and dominated the ecology of metastatic LUAD during metastatic evolution. IGF2BP2 was preferentially secreted by exosomes in the LUAD_IGF2BP2 subpopulation, which was absorbed by the En_IGF2BP2 subpopulation in the tumor microenvironment. Subsequently, IGF2BP2 improved the RNA stability of FLT4 through m6A modification, thereby activating the PI3K-Akt signaling pathway, and eventually promoting angiogenesis and metastasis. Analysis of clinical data showed that IGF2BP2 was linked with poor overall survival and relapse-free survival for LUAD patients. CONCLUSIONS: Overall, these findings provide a novel insight into the multicellular ecosystems of primary and metastatic LUAD, and demonstrate that a specific LUAD_IGF2BP2 subpopulation is a key orchestrator promoting angiogenesis and metastasis, with implications for the gene regulatory mechanisms of LUAD metastatic evolution, representing themselves as potential antiangiogenic targets.

Natural variation in the HAN1 gene confers chilling tolerance in rice and allowed adaptation to a temperate climate.

Rice (Oryza sativa L.) is a chilling-sensitive staple crop that originated in subtropical regions of Asia. Introduction of the chilling tolerance trait enables the expansion of rice cultivation to temperate regions. Here we report the cloning and characterization of HAN1, a quantitative trait locus (QTL) that confers chilling tolerance on temperate japonica rice. HAN1 encodes an oxidase that catalyzes the conversion of biologically active jasmonoyl-L-isoleucine (JA-Ile) to the inactive form 12-hydroxy-JA-Ile (12OH-JA-Ile) and fine-tunes the JA-mediated chilling response. Natural variants in HAN1 diverged between indica and japonica rice during domestication. A specific allele from temperate japonica rice, which gained a putative MYB cis-element in the promoter of HAN1 during the divergence of the two japonica ecotypes, enhances the chilling tolerance of temperate japonica rice and allows it to adapt to a temperate climate. The results of this study extend our understanding of the northward expansion of rice cultivation and provide a target gene for the improvement of chilling tolerance in rice.

Organ-Specific Metabolic Shifts of Flavonoids in Scutellaria baicalensis at Different Growth and Development Stages.

Scutellaria baicalensis Georgi is a traditional Chinese herbal medicine mainly containing flavonoids that contribute to its bioactivities. In this study, the distributions and dynamic changes of flavonoid levels in various organs of S. baicalensis at different development stages were investigated by UHPLC-QTOF-MS/MS and HPLC-DAD methods. The results indicated that the metabolic profiles of S. baicalensis changed with growth and development. During the initial germination stage, the seeds mainly contained flavonols. With growth, the main kinds of flavonoids in S. baicalensis changed from flavonols to flavanones and flavones. The results also revealed that the accumulation of flavonoids in S. baicalensis is organ-specific. The flavones without 4'-OH groups mainly accumulate in the root and the flavanones mainly accumulate in aerial organs. Dynamic accumulation analysis showed that the main flavonoids in the root of S. baicalensis accumulated rapidly before the full-bloom stage, then changed to a small extent. The results suggested the proper harvest time for the aerial parts was at the initial stage of reproductive growth and the flower buds should be collected before flowering. This study deepening the knowledge of S. baicalensis should provide valuable information for guiding the scientific cultivation of this plant and the development and utilization of S. baicalensis.

Effects of graded emergency nursing on resuscitation outcomes, prognosis, and nursing satisfaction in patients with acute myocardial infarction.

OBJECTIVE: This study was conducted to explore the effect of graded emergency nursing on the resuscitation outcomes, prognosis and nursing satisfaction of patients with acute myocardial infarction (AMI). METHOD: Ninety-five patients with AMI admitted to the emergency department of our hospital from May 2018 to May 2020 were enrolled as the study subjects and were randomly divided into the control group (n=47) and the experimental group (n=48). Patients in the control group received routine care, and patients in the experimental group received graded nursing. The two groups were compared in terms of resuscitation outcomes, prognosis and nursing satisfaction. RESULTS: After intervention, the waiting time, door-to-triage time and treatment duration of the experimental group were significantly shorter than those of the control group (P<0.05). Patients in the experimental group had fewer complications such as shock, cardiac arrhythmia, chest pain, etc.; higher scores of Karnofsky Performance Scale (KPS) and quality of life (QOL) (P<0.05); and higher scores of nursing satisfaction than the control group (P<0.05). CONCLUSION: Graded nursing for AMI patients during emergency care can shorten the waiting and triage time, improve the success rate of resuscitation, reduce the incidence of complications, improve the prognosis and nursing satisfaction of patients.

Influence of targeted motivational interviewing on self-care level and prognosis during nursing care of chronic heart failure.

OBJECTIVE: To analyze the effects of targeted motivational interviewing (TMI) during the nursing care for chronic heart failure (CHF). METHODS: A total of 93 patients with CHF admitted to our hospital were selected as study subjects, and were divided into Group A (n=47) and Group B (n=46) in accordance with a randomized double-blind method. Group A was treated with TMI, while Group B was treated with the conventional nursing care for heart failure (HF). The self-care levels (self-care of heart failure index), medication compliance (Morisky's medication compliance scale), quality of life (Minnesota Living with Heart Failure Questionnaire), and prognosis (the readmission and mortality rates) were compared between the 2 groups. RESULTS: After nursing care, patients in Group A were higher than those in Group B in their scores of self-care maintenance, management and confidence, medication frequency, time, dose, and type as instructed by the physician, and drug withdrawal or discontinuation of drugs, and forgetting medication, and body, emotion, and other fields (P < 0.05). Group A was superior to Group B in the rates of medication compliance and medication non-compliance (61.70% and 6.38% vs. 41.30% and 23.91%, P < 0.05); rate of hospital readmission (6.38% vs. 23.91%, P < 0.05), and mortality rate (0% vs. 6.52%, P > 0.05). CONCLUSION: TMI is conducive to improving the self-care level, medication compliance, quality of life, and prognosis in patients with CHF.