Spectral entropy as a measure of the metaproteome complexity.

The diversity and complexity of the microbiome's genomic landscape are not always mirrored in its proteomic profile. Despite the anticipated proteomic diversity, observed complexities of microbiome samples are often lower than expected. Two main factors contribute to this discrepancy: limitations in mass spectrometry's detection sensitivity and bioinformatics challenges in metaproteomics identification. This study introduces a novel approach to evaluating sample complexity directly at the full mass spectrum (MS1) level rather than relying on peptide identifications. When analyzing under identical mass spectrometry conditions, microbiome samples displayed significantly higher complexity, as evidenced by the spectral entropy and peptide candidate entropy, compared to single-species samples. The research provides solid evidence for the complexity of microbiome in proteomics indicating the optimization potential of the bioinformatics workflow.

Sulfonate Derivatives Containing a Kakuol Moiety as Potential Fungicidal Candidates: Design, Synthesis and Antifungal Activity Evaluation.

As a part of our continuing exploration to discover new potential promising fungicide candidates, eighteen sulfonate derivatives (3a-3r) containing a kakuol moiety were designed and synthesized. Synthetic sulfonate derivatives were tested comprehensively for antifungal activities against four plant pathogenic fungi (Botrytis (B.) cinerea, Valsa (V.) mali, Fusarium (F.) graminearum, Sclerotinia (S.) sclerotiorum), and their structure activity relationships were summarized. Especially, derivatives 3i and 3j exhibited remarkable activity against V. mali, with the inhibition rates of 99.8 and 100%, which were slightly superior to that of carbendazim (98.9%), a reference fungicide. Moreover, derivatives 3a, 3k and 3q possess the broader antifungal spectrum against three tested plant pathogenic fungi with inhibition rates over 60%. Structure-activity relationship (SAR) analysis indicated that the introduction of 2-F or 3-F into the benzene ring would give rise to a remarkable increase of the antifungal activity against V. mali.

Effects of simulated presence therapy on agitated behavior, cognition, and use of protective constraint among patients with senile dementia.

The research was conducted to investigate the improvement of agitated behaviors, cognitive functions, and negative emotions among patients with senile dementia and the burden of caregivers after simulated presence therapy (SPT) intervention. 85 Patients with senile dementia were included as the research subjects and divided into control group (40 cases performed with routine nursing) and observation group (45 cases undergoing routine nursing combined with SPT) via a random number table method. Cohen-Mansfield agitation inventory (CAMI) and protective constraint were used to assess the improvement of agitated behaviors among patients. Besides, apathy evaluation scale-informant (AES-I), functional independence measure (FIM), self-rating depression scale (SDS), self-rating anxiety scale (SAS), clock drawing test, and caregiver burden inventory (CBI) were adopted to evaluate the differences in apathy, daily living and sociability, depression, anxiety, cognitive functions, and caregiver burden between the two groups. It was demonstrated that CAMI score, the duration of protective constraint use, AES-I score, SDS score, SAS score, and CBI score among patients in observation group all apparently decreased compared with those in control group after the intervention. In contrast, FIM and clock drawing test scores both notably increased (p < 0.05). The above findings suggested that SPT could obviously reduce the incidence of agitated behaviors, improve the level of apathy, daily living and sociability, depression, anxiety, and cognitive functions, and relieve caregiver burden among patients with senile dementia during SPT intervention for patients with senile dementia.

Eating disorders symptoms and depressive symptoms in Chinese Tibetan University students: a network analysis.

BACKGROUND: Depression is being increasingly acknowledged as a global public health concern, and following this trend, attention towards eating disorders (EDs) has surged within China's national consciousness. EDs symptoms frequently coexist with various mental health conditions, including depression. However, research focusing on EDs symptoms and depressive symptoms among Tibetan students in China remains scant. This study employs network analysis to estimate the relational network between EDs and depressive symptoms. METHODS: Tibetan (n = 2,582) and Han (n = 1,743) students from two universities in the Xizang Autonomous Region, China, completed the Eating Attitude Test-26 (EAT-26) and the Patient Health Questionnaire-9 (PHQ-9). We estimated the network structure of EDs symptoms and depressive symptoms, identified central and bridge symptoms, and examined whether network characteristics differed by gender and ethnic. RESULTS: The core symptoms identified within this study were Calorie_awareness, Desire_to_thin and Fatigue. Conversely, bridge symptoms included Appetite, Suicide, Anhedonia, Guilty, Body_fat_awareness, and Food_preoccupation. The study also revealed no significant gender differences within the network model. However, disparities among ethnic groups were observed within the network structure. CONCLUSIONS: Our study examined the correlation between EDs symptoms and depressive symptoms in Tibetan college students. Focusing on the individual's quest for the perfect body shape and some Tibetan students' appetite problems - potentially stemming from transitioning to a new university environment, adapting to the school canteen's diet, or being away from their hometown - could aid in the prevention and management of EDs and depression symptoms. It could reduce the incidence of complications by helping students maintain good physical and mental health. Concurrently, our research provides insights into the relatively higher levels of depression triggered by the unique plateau environment.

Tunable and switchable multi-wavelength actively Q-switched random fiber laser based on an electro-optic modulator and Sagnac loop filter.

In this article, an actively Q-switched multi-wavelength random fiber laser based on a Sagnac loop filter and electro-optic modulator is proposed and demonstrated experimentally. The random distributed feedback media is a section of a 25 km long single-mode fiber. When the pump power is 350 mW, the polarization angle of the Sagnac loop filter can be adjusted by polarization controller to achieve the switching of a single, double, triple, and quadruple channels laser output. In the case of a single laser channel, dual laser channels, and three laser channels output, multiple laser channels can be tuned simultaneously with a fixed wavelength interval. In addition, by changing the waveform of the external signal source, the light and dark pulses can be switched. Owing to the half-open cavity structure and the high gain of the erbium-doped fiber, the laser threshold was reduced to 25 mW, and the light conversion efficiency was 0.67%. The laser is an ideal light source for medical imaging and long-distance sensing.

Secretory expression of mammalian NOTCH tandem epidermal growth factor-like repeats based on increased O-glycosylation.

The Notch pathway represents evolutionarily conserved intercellular signaling essential for cell-to-cell communication during development. Dysregulation of Notch signaling has been implicated in various diseases, and its control represents a potential cancer treatment strategy. Notch signaling is initiated by the interaction of NOTCH receptors with their ligands on neighboring cells. Therefore, the truncated NOTCH ectodomain, composed mainly of tandem repeats of epidermal growth factor-like (EGF) domains, serves as a decoy molecule that competes for ligand binding and thus inhibits ligand-dependent Notch signaling. Although full-length NOTCH EGF repeats exhibited potent Notch inhibitory activity, they were poorly produced in the transfected cells. This study evaluated the effect of EGF domain-modifying glycosyltransferases on the secretion of NOTCH EGF repeats. Our results in HEK293T cells revealed that, unlike the effect on endogenous NOTCH receptors, overexpressed EGF domain-specific O-GlcNAc transferase (EOGT) markedly enhanced the secretion of NOTCH1 EGF repeats in an enzyme activity-dependent manner. The co-expression of protein O-glucosyltransferase 1 further manifested the effect of EOGT. The resultant changes in O-glycosylation of NOTCH3 were evaluated by label-free glycopeptide quantification. This study provides an experimental strategy to efficiently generate NOTCH EGF repeats by manipulating the expression of glycosyltransferases that alter the O-glycosylation of EGF domains.

Tunable and switchable multi-wavelength random distributed feedback fiber laser based on SMF and a cascaded Sagnac ring filter.

A tunable and switchable multi-wavelength random distributed feedback fiber laser based on cascaded Sagnac loops is proposed and experimentally demonstrated. The random distribution feedback of the laser is provided by the Rayleigh scattering generated by the single-mode fiber (SMF). The cascaded Sagnac loops act as a filter and a reflector in the half-open cavity laser. The single-, dual-, three-, and four-wavelength channels can be realized by adjusting the angle of the polarization controller at the pump power of 300 mW. In the single-, dual-, and three-wavelength channels, the wavelength spacing can be maintained, and the laser wavelength position can be changed at the same time. The maximum wavelength tuning ranges of single-, dual-, and three-wavelength outputs are about 4.5 nm, 2.6 nm, and 1 nm, respectively. The proposed multi-wavelength random fiber laser has the advantages of simple structure and low threshold, and it has good application prospects in remote sensing and imaging systems.

Research on the drought tolerance mechanism of Pennisetum glaucum (L.) in the root during the seedling stage.

BACKGROUND: Drought is one of the major environmental stresses resulting in a huge reduction in crop growth and biomass production. Pearl millet (Pennisetum glaucum L.) has excellent drought tolerance, and it could be used as a model plant to study drought resistance. The root is a very crucial part of plant that plays important roles in plant growth and development, which makes it a focus of research. RESULTS: In this study, we explored the mechanism of drought tolerance of pearl millet by comparing physiological and transcriptomic data under normal condition and drought treatment at three time points (1 h, 3 h and 7 h) in the root during the seedling stage. The relative electrical conductivity went up from 1 h to 7 h in both control and drought treatment groups while the content of malondialdehyde decreased. A total of 2004, 1538 and 605 differentially expressed genes were found at 1 h, 3 h and 7 h respectively and 12 genes showed up-regulation at all time points. Some of these differentially expressed genes were significantly enriched into 'metabolic processes', 'MAPK signaling pathway' and 'plant hormone signal transduction' such as the ABA signal transduction pathway in GO and KEGG enrichment analysis. CONCLUSIONS: Pearl millet was found to have a quick drought response, which may occur before 1 h that contributes to its tolerance against drought stress. These results can provide a theoretical basis to enhance the drought resistance in other plant species.

Circular RNA Circ-BANP Regulates Oxidized Low-density Lipoprotein-induced Endothelial Cell Injury Through Targeting the miR-370/Thioredoxin-interacting Protein Axis.

ABSTRACT: Dysfunction of endothelial cells is now recognized as an important contributor to the pathogenesis of atherosclerosis (AS). Circular RNAs (circRNAs) have been demonstrated to be involved in AS pathogenesis. The purpose of this study was to explore the biological action of circRNA BTG3-associated nuclear protein (circ-BANP, hsa_circ_0040824) on the dysfunction of human umbilical vein endothelial cells (HUVECs) induced by oxidized low-density lipoprotein (ox-LDL). The levels of circ-BANP, miR-370, and thioredoxin-interacting protein (TXNIP) were gauged by quantitative real-time polymerase chain reaction or Western blot. The subcellular fractionation assay was used to determine the localization of circ-BANP, and the ribonuclease R assay was performed to evaluate the stability of circ-BANP. Cell viability, apoptosis, migration, invasion, and tube formation abilities were assessed by the Cell Counting Kit-8, flow cytometry, transwell, and tube formation assays. The levels of interleukin-6, tumor necrosis factor-α, and interleukin-1ß were detected by enzyme-linked immunosorbent assay. Targeted relationships among circ-BANP, miR-370, and TXNIP were confirmed by a dual-luciferase reporter assay. Our data showed that circ-BANP expression was upregulated in AS blood and ox-LDL-induced HUVECs. The inhibition of circ-BANP promoted cell viability, migration, invasion, tube formation, and repressed cell inflammation and apoptosis in ox-LDL-induced HUVECs, demonstrating that circ-BANP silencing alleviated ox-LDL-induced HUVEC injury. Mechanistically, circ-BANP directly targeted miR-370. Moreover, miR-370 mediated the regulation of circ-BANP in ox-LDL-induced cell injury in HUVECs. TXNIP was a target of miR-370, and miR-370 overexpression relieved ox-LDL-induced HUVEC injury by downregulating TXNIP. Furthermore, circ-BANP modulated TXNIP expression by targeting miR-370. Our findings demonstrated that circ-BANP regulated ox-LDL-induced cell injury in HUVECs at least in part through targeting the miR-370/TXNIP axis, illuminating circ-BANP as a potential target for AS detection and treatment.

Association of lipid profiles with severity and outcome of acute ischemic stroke in patients with and without chronic kidney disease.

BACKGROUND: Contribution of lipid profiles to stroke severity and outcome was inconclusive, whether chronic kidney disease (CKD) (estimated glomerular filtration rate < 60 mL/min/1.73 m2) affects the association has not been investigated. We aim to evaluate this relationship. METHODS: A retrospective study of consecutive acute ischemic stroke patients was performed. We assessed the risk of severe stroke with the National Institutes of Health Stroke Scale (NIHSS) ≥ 5 at admission and poor outcome with the modified Rankin Scale (mRS) ≥ 3 at discharge. Multivariate stepwise logistic regression models were adopted to study interaction and independent association of lipid components with stroke severity and outcome according to lipid level quartiles by CKD stratification. RESULTS: Among the 875 included patients (mean age 64.9 years, 67.8% males), 213 (24.3%) presented with CKD. Elevated low-density lipoprotein cholesterol (LDL-C) was independently associated with severe stroke in patients with CKD (P for trend = 0.033) than in those without CKD (P for trend = 0.121). The association between the level of LDL-C and stroke severity was appreciably modified by CKD (Pinteraction = 0.013). Compared with without CKD patients in the lowest LDL-C quartile, the multivariable-adjusted risk of severe stroke increased significantly by 2.9-fold (95% CI 1.48-5.74) in patients with CKD in the highest LDL-C quartile. No significant association was observed between lipid components and early outcome in patients with and without CKD. CONCLUSION: LDL-C levels are positively associated with stroke severity in only patients with CKD, with an interactive impact of LDL-C and CKD on ischemic stroke in the acute phase.

Unmet health needs and associated factors among 1727 rural community-dwelling older adults: A cross-sectional study.

AIM: To investigate the needed, unneeded and unmet health care services among rural community-dwelling older adults in China and examine the influencing factors, aiming to facilitate the global development of the home care system for a healthier world. METHODS: This cross-sectional study investigated rural areas in three provinces in Western China. A total of 1727 rural community-dwelling older adults were enrolled. The needed, unneeded and unmet health care services were assessed by the Supply and Demand of Health Care Services (SD-HCS) questionnaire for older adults. RESULTS: Respect (73%, 1265/1727) was the most needed. The other top 9 needed mainly belonged to the divisions of health monitoring and information regarding chronic diseases. Re-employment or part-time jobs (71%, 1230/1727) was the most unneeded. All five protection and safety items were the most unmet but needed. Religion was the main influencing factor of those health care services that were less unmet but needed. CONCLUSIONS: Respect was basic for older adults, and chronic disease management was in great demand among rural community-dwelling older adults. Due to low willingness and the crisis workforce, a more flexible retirement policy is needed in rural China. It is urgent to improve the emergency care system in rural areas. Last but not least, more research is needed to explore the association between religion and health.

Ultrahigh-activity immune inducer from Endophytic Fungi induces tobacco resistance to virus by SA pathway and RNA silencing.

BACKGROUND: Plant viruses cause severe economic losses in agricultural production. An ultrahigh activity plant immune inducer (i.e., ZhiNengCong, ZNC) was extracted from endophytic fungi, and it could promote plant growth and enhance resistance to bacteria. However, the antiviral function has not been studied. Our study aims to evaluate the antiviral molecular mechanisms of ZNC in tobacco. RESULTS: Here, we used Potato X virus (PVX), wild-type tobacco and NahG transgenic tobacco as materials to study the resistance of ZNC to virus. ZNC exhibited a high activity in enhancing resistance to viruses and showed optimal use concentration at 100-150 ng/mL. ZNC also induced reactive oxygen species accumulation, increased salicylic acid (SA) content by upregulating the expression of phenylalanine ammonia lyase (PAL) gene and activated SA signaling pathway. We generated transcriptome profiles from ZNC-treated seedlings using RNA sequencing. The first GO term in biological process was positive regulation of post-transcriptional gene silencing, and the subsequent results showed that ZNC promoted RNA silencing. ZNC-sprayed wild-type leaves showed decreased infection areas, whereas ZNC failed to induce a protective effect against PVX in NahG leaves. CONCLUSION: All results indicate that ZNC is an ultrahigh-activity immune inducer, and it could enhance tobacco resistance to PVX at low concentration by positively regulating the RNA silencing via SA pathway. The antiviral mechanism of ZNC was first revealed in this study, and this study provides a new antiviral bioagent.

Transcriptome analysis of heat stress and drought stress in pearl millet based on Pacbio full-length transcriptome sequencing.

BACKGROUND: Heat and drought are serious threats for crop growth and development. As the sixth largest cereal crop in the world, pearl millet can not only be used for food and forage but also as a source of bioenergy. Pearl millet is highly tolerant to heat and drought. Given this, it is considered an ideal crop to study plant stress tolerance and can be used to identify heat-resistant genes. RESULTS: In this study, we used Pacbio sequencing data as a reference sequence to analyze the Illumina data of pearl millet that had been subjected to heat and drought stress for 48 h. By summarizing previous studies, we found 26,299 new genes and 63,090 new transcripts, and the number of gene annotations increased by 20.18%. We identified 2792 transcription factors and 1223 transcriptional regulators. There were 318 TFs and 149 TRs differentially expressed under heat stress, and 315 TFs and 128 TRs were differentially expressed under drought stress. We used RNA sequencing to identify 6920 genes and 6484 genes differentially expressed under heat stress and drought stress, respectively. CONCLUSIONS: Through Pacbio sequencing, we have identified more new genes and new transcripts. On the other hand, comparing the differentially expressed genes under heat tolerance with the DEGs under drought stress, we found that even in the same pathway, pearl millet responds with a different protein.

Icotinib is as efficacious as gefitinib for brain metastasis of EGFR mutated non-small-cell lung cancer.

BACKGROUND: The prognosis of non-small-cell lung cancer (NSCLC) with brain metastases is very poor. Currently, therapeutic methods for this patient population include whole-brain radiation therapy (WBRT), surgery, radiosurgery and systemic treatment. Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) could be effective on cerebral metastases of mutated NSCLC. However, which EGFR-TKIs is more appropriate is still unknown. METHODS: We conducted a retrospective analysis of advanced NSCLC patients with brain metastases for EGFR targeted therapy from November 2013 to April 2018 at Dongguan People's Hospital, Southern Medical University, China. A total of 43 patients were recruit in this study. Among them, 21 cases received icotinib (125 mg, thrice a day) and 22 cases received gefitinib (250 mg, once a day) until disease progression or unacceptable toxicity. The primary end point of this study was intracranial PFS (iPFS). The relationships between therapeutic arms and patients characteristics were performed using Pearson's chi-square test or Fisher's exact test. The differences in PFS among the two arms were analyzed using Kaplan-Meier curves and log rank tests. RESULTS: There was no significant difference of intracranial ORR (66.6% versus 59.1%, P = 0.62) and DCR (85.7% versus 81.8%, P = 0.73) between the two arms. The median intracranial PFS (iPFS) for icotinib and gefitinib arms were 8.4 months (95% CI, 5.4 to 11.3 months) and 10.6 months (95% CI, 6.3 to 14.8 months), respectively (P = 0.17). Adverse events of the two study arms were generally mild. None of the patients experienced dose reduction of EGFR-TKIs. CONCLUSIONS: Our study showed that icotinib and gefitinib had similar efficacy for brain metastasis of EGFR mutated NSCLC. Large randomized studies are suggested to further illuminate the effect of these two EGFR-TKIs on cerebral lesions of NSCLC.

Evaluation of genetic integrity of pearl millet seeds during aging by genomic-SSR markers.

Seed is an important way to store germplasm resources but its genetic integrity will decrease during long-term preservation. So, it's essential to update seeds according to the aging level of different species. Pearl millet [Cenchrus americanus (L.) Morrone syn., Pennisetum glaucum (L.) R. Br.] is a crucial forage grass, biofuel plant and important crops in the world bringing huge economic and ecological benefits. However, there is no report about the impact of aging on genetic integrity of its seeds. In this study, four genetic diversity indexes (the percentage of polymorphic bands, PPB; the effective number of alleles, Ne; the Nei's gene diversity index, H; the Shannon's information index, I) and 20 pairs of genomic-SSR primers were used to certify the optimal sample volume of pearl millet for molecular study and found that the best sample volume was 60. After the artificial aging test, the germination rate and four genetic diversity parameters (the number of alleles, Na; Ne; H; I) were used to evaluate the change of genetic integrity at different aging levels. The results showed that the germination rate and these four genetic diversity parameters declined with the increase of aging levels. Furthermore, when the germination rate of pearl millet seeds went down to 68.23%, a significant difference in genetic integrity was observed with unaged seeds. In conclusion, the optimal sample size of pearl millet was 60 and the critical point of germination rate to renew germplasm resources was 68.23% and these finds might contribute to the scientific study and the safe conservation of pearl millet.

Facile Synthesis of 3D-Structured NiS as an Excellent Fenton-like Catalyst under Various pH through Different Mechanisms.

Two-dimensional (2D) layer-structured transition-metal sulfide shows excellent Fenton catalytic properties, which has attracted much attention. However, the release of metal cations from the catalyst induces secondary environmental pollution. In the present study, 3D-constructed NiS assembled by 2D nanosheets was synthesized by a hydrothermal method. The NiS displayed high levels of Fenton catalytic activity for the degradation of rhodamine B (RhB) under a wide pH range from 5.60 to 12.21. However, Ni2+ was released in acidic solutions, and the released Ni2+ decreased with increasing pH. The catalytic mechanisms in acidic and alkaline solutions were explored. The high Fenton catalytic activity benefits from the synergistic effect of the Ni2+ and the S2- at pH = 5.60. At pH = 12.21, the S atom accelerated the electron transfer between the Ni(III) and Ni(II) at the NiS surface. The material kept the same catalytic activity for many cycles. These results demonstrate that NiS can be used as a catalyst for the Fenton process in alkaline conditions.

Reference gene selection for real-time quantitative PCR normalization in Hemarthria compressa and Hemarthria altissima leaf tissue.

Hemarthria compressa and Hemarthria altissima are widely used as livestock forage and play important roles in tropical and subtropical grassland agricultural systems promoting healthy ecological environment and the development of animal husbandry. Leaf tissue of "Yaan" limpograss (H. compressa) and "H255" whip grass (H. altissima) were used to test the mRNA expression levels of 12 reference genes using RT-qPCR. The Delta-Ct method, BestKeeper (ver. 1.0), Genorm (ver. 3.5), Normfinder (ver. 0.953) and RefFinder were used to analyze the expression stability of the 12 reference genes under drought, salt, acid-aluminum and cold stresses to provide significant technical support for the study of gene expression under various abiotic stresses in Hemarthria. The results showed that the candidate reference genes showed divergent expression levels under various abiotic stresses. Among the genes that were selected, CL18892 showed the highest expression stability under salt stress in the leaf tissue. eEF-1α was the most stable gene under cold and acid-aluminum stresses and CL16384 was comparatively the most suitable genes under drought stress. As a whole, according to RefFinder analysis, CYP5, BMK.74327 and CL21527 were the most suitable reference genes for studying the effects of abiotic stress in Hemarthria. In general, CL16812 and CL18038 were not suitable reference genes under abiotic stress conditions that were examined in this study.

Mach-Zehnder fiber sensing and positioning system based on common optical path technology.

A Mach-Zehnder interferometer (MZI) fiber sensing and positioning system based on common optical path technology that combines the advantages of the positioning method of the conventional MZI and the common optical path technology is proposed to improve the performance of the system in this paper. The feasibility of the interferometer has been proved by simulation and experiment. In the conventional MZI, the temperature and environment have an effect on the system performance due to the interference light transmitted through different optical fibers. The system proposed in this paper overcomes the disadvantages of the conventional MZI.

Effect of metformin on glycaemic control in patients with type 1 diabetes: A meta-analysis of randomized controlled trials.

BACKGROUND: For type 1 diabetes (T1D) patients, adding metformin to insulin therapies is thought to improve blood glucose levels, but current evidence does not support this clinical benefit. Additional data from large clinical trials are now available; therefore, we conducted a meta-analysis of studies on assessing the efficacy and adverse effects of metformin. METHODS: We searched the MEDLINE, EMBASE, and Cochrane Library databases for data from randomized controlled trials. We performed statistical analyses by using Review Manager 5.2. RESULTS: Thirteen randomized controlled trials that compared metformin versus placebo met our inclusion criteria and were included in the study. The final meta-analysis included a total of 1183 participants with T1D. Metformin was associated with reductions in BMI (-1.14, 95% CI -2.05 to -0.24, P = .01), insulin requirements (-0.47, 95% CI -0.70 to -0.23, P = .0001), total cholesterol (-0.23, 95% CI -0.34 to -0.12, P < .0001), and low-density lipoprotein cholesterol (-0.20, 95% CI -0.29 to -0.11, P < .0001) in T1D patients. No clear evidence indicated that metformin improved HbA1c, triglyceride, or high-density lipoprotein cholesterol levels. A safety analysis showed that metformin slightly increased the risk of severe hypoglycaemia (1.23, 95% CI 1.00 to 1.52, P = .05) and mainly gastrointestinal adverse events (2.67, 95% CI 2.06 to 3.45, P < .00001). No evidence showed that metformin increased diabetic ketoacidosis events. CONCLUSIONS: Compared with placebo, metformin was not associated with glycaemic control in T1D patients. Although it exhibited other benefits, such as lower BMI and reduced insulin requirements, total cholesterol, and low-density lipoprotein cholesterol, negative outcomes, such as gastrointestinal adverse effects and severe hypoglycaemia, should also be considered in the use of metformin for T1D patients.

Interferometric sensor with a PGC-AD-DSM demodulation algorithm insensitive to phase modulation depth and light intensity disturbance.

An interferometric sensor with asymmetric division and a differential-self-multiplication phase-generated carrier (PGC-AD-DSM) demodulation algorithm based on fundamental frequency mixing is proposed and experimentally demonstrated. The effect of carrier modulation depth C on the demodulation result is eliminated via DSM. The influence of light intensity disturbance on the demodulation result is also eliminated by asymmetric division. In experiment, the proposed algorithm eliminates the influence of C on the demodulation result. The signal-to-noise and distortion ratio of the sensor with the proposed algorithm achieves a gain of 7.77 dB over the PGC-Arctan algorithm and 9.48 dB over the PGC-DCM algorithm.