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1.
Beijing Da Xue Xue Bao Yi Xue Ban ; 47(4): 667-73, 2015 Aug 18.
Artículo en Zh | MEDLINE | ID: mdl-26284407

RESUMEN

OBJECTIVE: To compare the diffusion properties of fluorescent probes dextran-tetramethylrhodamine (DT) and lucifer yellow CH (LY) and magnetic probe gadolinium-diethylene triamine pentaacetic acid (Gd-DTPA) in porous media and to screen out a suitable fluorescent probe for optical imaging of brain interstitial space (ISS). METHODS: Agarose gels sample were divided into DT group, LY group and Gd-DTPA group, and the corresponding molecular probes were imported in each group. The dynamic diffusions of DT and LY in agarose gels at different time points (15, 30, 45, 60, 90, and 120 min) were scanned with laser scanning confocal microscope, the dynamic diffusion of Gd-DTPA was imaged with magnetic resonance imaging. The average diffusion speed of LY were demonstrated to be consistent with those of Gd-DTPA. The LY was introduced into caudate putamen of 18 rats, respectively, the diffusion of LY in the sequential slices of rat brain at different time points (0.5, 1, 2, 3, 7, 11 h) were scanned, and the results were compared with those of rats' brain with Gd-DTPA imported and imaged in vivo with magnetic resonance imaging. RESULTS: The diffusions of the three probes were isotropic in the agarose gels, and the average diffusion speeds of DT, LY and Gd-DTPA were: (0.07±0.02)×10(-2) mm2/s, (1.54±0.47)×10(-2) mm2/s, (1.45±0.50)×10(-2) mm2/s, respectively. The speed of DT was more slower than both LY and Gd-DTPA (ANOVA, F=367.15, P<0.001; Post-Hoc LSD, P<0.001), and there was no significant difference between the speeds of LY and Gd-DTPA (Post-Hoc LSD, P=0.091). The variation tendency of diffusion area of DT was different with both that of LY and that of Gd-DTPA (Bonferroni correction, α=0.0125, P<0.001), and there was no significant difference between LY and Gd-DTPA (Bonferroni correction, α=0.0125, P=0.203), in analysis by repeated measures data of ANOVA. The diffusions of LY and Gd-DTPA were anisotropy in rat caudate putamen,and the average diffusion speeds of LY and Gd-DTPA were: (1.03±0.29)×10(-3) mm2/s, (0.81±0.27)×10(-3) mm2/s, respectively, no significant difference was demonstrated (t=0.759, P=0.490); half-time of single intensity of LY and Gd-DTPA was (2.58±0.04) h, (2.46±0.10) h, respectively, no significant difference was found (t=2.025, P=0.113). The diffusion area ratios between LY and Gd-DTPA in rat caudate putamen was not statistically different at hours 0.5, 1, 2, 3 and 7 (t=2.249, P=0.088; t=2.582, P=0.061; t=1.966, P=0.121; t=0.132, P=0.674; t=0.032, P=0.976), while, a slightly difference was found at 11 h (t=2.917, P=0.043,in analysis by t test). CONCLUSION: LY present the same diffusion property with Gd-DTPA in porous media witch including agarose gels and live rat brain tissue, indicates that LY is a suitable fluorescent probe for optical imaging of brain ISS, and it can be used for microscopic, macro and in vitro measure of brain ISS.


Asunto(s)
Medios de Contraste , Colorantes Fluorescentes , Sondas Moleculares , Neuroimagen , Animales , Encéfalo , Difusión , Fluorescencia , Gadolinio DTPA , Imagen por Resonancia Magnética , Microscopía Confocal , Ratas
2.
Thorac Cancer ; 13(6): 858-869, 2022 03.
Artículo en Inglés | MEDLINE | ID: mdl-35142041

RESUMEN

BACKGROUND: Accumulating studies have suggested the airway microbiota in lung cancer patients is significantly different from that of healthy controls. However, little is known about the relationship between airway microbiota and important clinical parameters of lung cancer. In this study, we aimed to explore the association between sputum microbiota and lung cancer stage, lymph node metastasis, intrathoracic metastasis, and epidermal growth factor receptor (EGFR) gene mutation. METHODS: The microbiota of sputum samples from 85 newly-diagnosed NSCLC patients were sequenced via 16S rRNA sequencing of the V3-V4 region. Sequencing reads were filtered using QIIME2 and clustered against UPARSE. RESULTS: Alpha- and ß-diversity was significantly different between patients in stages I to II (early stage, ES) and patients in stages III to IV (advanced stage, AS). Linear discriminant analysis Effect Size (LEfSe) identified that genera Granulicatella and Actinobacillus were significantly enriched in ES, and the genus Actinomyces was significantly enriched in AS. PICRUSt2 identified that the NAD salvage pathway was significantly enriched in AS, which was positively associated with Granulicatella. Patients with intrathoracic metastasis were associated with increased genus Peptostreptococcus and incomplete reductive TCA cycle, which was associated with increased Peptostreptococcus. Genera Parvimonas, Pseudomona and L-valine biosynthesis were positively associated with lymph node metastasis. L-valine biosynthesis was related with increased Pseudomona. Finally, the genus Parvimonas was significantly enriched in adenocarcinoma patients with EGFR mutation. CONCLUSION: The taxonomy structure differed between different lung cancer stages. The tumor stage, intrathoracic metastasis, lymph node metastasis, and EGFR mutation were associated with alteration of specific airway genera and metabolic function of sputum microbiota.


Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas , Neoplasias Pulmonares , Microbiota , Carcinoma de Pulmón de Células no Pequeñas/genética , Carcinoma de Pulmón de Células no Pequeñas/patología , Receptores ErbB/genética , Genes erbB-1 , Humanos , Pulmón/patología , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patología , Mutación , ARN Ribosómico 16S/genética
3.
Huan Jing Ke Xue ; 41(5): 2017-2025, 2020 May 08.
Artículo en Zh | MEDLINE | ID: mdl-32608818

RESUMEN

The hygroscopicity of atmospheric particulate matter can determine its optical behaviors, cloud condensation nuclei activity, and heterogeneous chemical reaction on the surface. However, the effect of secondary organic matter on the hygroscopicity of inorganic salts is still rarely studied. In this study, a 2 L gas-phase flow tube was used to stimulate the oxidation process of α-pinene. NaCl particles at the end of the flow tube served as seeds to absorb the product of α-pinene oxidation. The absorption times were 11, 15, and 20 h. Transmission electron microscopy results show that this system could produce particles with typical core-shell structure, and that the thickness of the organic shell would be thicker with longer collection time. Results from individual particle hygroscopic systems indicate that the organic shell can affect the deliquescent point of NaCl (pure NaCl deliquesces at 77%):for 11 h collection time, the NaCl core began to deliquesce at 75.5% relative humidity, and completely deliquesced at 78%; for 15 h collection time, the deliquescent range of the NaCl core was 75%-78.5%; and the deliquescent range was 76%-83% for the NaCl core with 20 h collection time. Furthermore, the organic shell could affect the hygroscopic growth of the NaCl core:the growth factor of the NaCl core with 11 h, 15 h, and 20 h collection time was 2.5, 2.3, and 1.8, respectively. These results indicate that:① the secondary organic shell could cause water absorption of the NaCl core in advance:② the organic shell could postpone the completely deliquescent point of the NaCl core, and this effect is more obvious with a thicker organic shell; ③ the organic shell could restrain the hygroscopic growth factor of the NaCl core. This study suggests that it is necessary to consider the influence of secondary organic matter on inorganic salts when studying the hygroscopic properties of atmospheric particles in climate models and field observations.

4.
Bioorg Med Chem ; 10(12): 3899-904, 2002 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-12413841

RESUMEN

7-Arylseleno-7-deoxydaunomycinone derivatives 3a-e and 7-thiophenyl-7-deoxydaunomycinones (7 and 8) were synthesized and the antitumor activities of them were evaluated against human stomach cancer SGC-7901 and human leukaemia HL60. The cytotoxic assay show that seleno daunomycinone derivatives are much better inhibitory activity than thiodaunomycinone and the structure-activity relationship was discussed. 7-Deoxydaunomycinone 4 was obtained when selenophenols were used in excess and the possible mechanism was proposed.


Asunto(s)
Antibióticos Antineoplásicos/síntesis química , Daunorrubicina/análogos & derivados , Daunorrubicina/síntesis química , Derivados del Benceno/química , Supervivencia Celular/efectos de los fármacos , Diseño de Fármacos , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Concentración 50 Inhibidora , Compuestos de Organoselenio/química , Relación Estructura-Actividad , Compuestos de Sulfhidrilo/química , Células Tumorales Cultivadas
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