[Effects of laser combined with periodontal basic treatment on periodontal indices, subgingival microflora, adiponectin, MMP-13 and IL-1ß in gingival crevicular fluid in patients with periodontitis].

PURPOSE: To investigate the effects of laser combined with periodontal basic treatment on periodontal indices, subgingival flora, adiponectin, matrix metalloproteinase-13 (MMP-13) and interleukin-1ß (IL-1ß) in patients with periodontitis. METHODS: A retrospective analysis was performed on 100 patients with periodontitis diagnosed and treated in Hengshui People's Hospital from December 2022 to July 2023. According to treatment methods, the patients were divided into control group (n=51) and experimental group (n=49). The control group received periodontal basic treatment, and the experimental group received laser treatment on the basis of the control group. The periodontal indexes, subgingival microflora, adiponectin, MMP-13, IL-1ß and bone metabolic factors of gingival crevicular fluid before and after treatment were compared between the two groups, as well as the clinical therapeutic effect. Statistical analysis was performed with SPSS 22.0 software package. RESULTS: After treatment, probing depth(PD), bleeding on probing(BOP), gingival index(GI) and plaque index (PLI) in the experimental group were lower than before treatment (P＜0.05), PD, BOP and PLI in the control group were lower than before treatment (P＜0.05), and PD, BOP, GI and PLI in the experimental group were significantly lower than those in control group (P＜0.05). After treatment, Lactobacillus, Clostridium and Bacteroides in both groups were significantly lower than before treatment (P＜0.05), and the experimental group was significantly lower than the control group(P＜0.05). After treatment, adiponectin in gingival crevicular fluid increased in both groups compared with before treatment(P＜0.05), and MMP-13 and IL-1ß in gingival crevicular fluid decreased in both groups compared with before treatment (P＜0.05), and adiponectin in gingival crevicular fluid in the experimental group was significantly higher than that in the control group (P＜0.05), MMP-13 and IL-1ß in the experimental group were significantly higher than that in the control group (P＜0.05). After treatment, procollagenⅠtype N-terminal peptide (PINP), cross linked C-telopeptide of type Ⅰ collagen(CXT) and bone glaprotein (BGP) were significantly higher than those before treatment (P＜0.05), and the experimental group was significantly higher than the control group (P＜0.05). The total effective rate of the experimental group was significantly higher than that of the control group (P＜0.05). CONCLUSIONS: Laser combined with periodontal basic treatment can effectively improve periodontal indexes, reduce subgingival flora, increase the levels of adiponectin and bone metabolic factor in gingival crevicular fluid, reduce the levels of MMP-13 and IL-1ß in gingival crevicular fluid, and improve the clinical therapeutic effect in patients with periodontitis.

UV light-induced self-assembly of gold nanocrystals into chains and networks in a solution of silver nitrate.

Controllable assemblies of nanocrystals have attracted considerable interest because they often exhibit unique collective properties that differ from those displayed by individual nanocrystals and bulk samples. Reported approaches to prepare nanocrystal assemblies based on the molecular recognitions of small molecules or biomacromolecules are effective, but often require complicated and time-consuming modification processes of nanocrystals. In this paper, we demonstrate a simple and universal approach to assemble gold nanocrystals (AuNCs) into linear chains and complex networks in aqueous silver nitrate medium under irradiation with UV light without the involvement of any modification step. Due to the strong plasmon resonance coupling verified by finite difference time domain calculation, the assembled structures of AuNCs can be used as excellent surface-enhanced Raman scattering substrates and dark-field light-scattering bioimaging probes.