Effects of autologous SCF- and G-CSF-mobilized bone marrow stem cells on hypoxia-inducible factor-1 in rats with ischemia-reperfusion renal injury.

To explore the mechanism whereby stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) jointly mobilize bone marrow stem cells (BMSCs) and promote kidney repair, male Sprague-Dawley rats were randomly assigned into 4 groups. In the treatment control group, rats were administered SCF (200 µg·kg(-1)·day(-1)) and G-CSF (50 µg·kg-1·day-1) for 5 days. In the treatment group, RIRI models were established, and 6 h later, SCF (200 µg·kg(-1)·day(-1)) and G-CSF (50 µg·kg(-1)·day(-1)) were administered for 5 days. In the model and treatment groups, tubular epithelial cell degeneration and necrosis were noticed, but the extent of repair in the treatment group was significantly better than in the model group. Five days after the operation, renal tissue CD34+ cells significantly increased in the model and treatment groups compared with the control and treatment control groups. HIF-1α, VEGF, and EPO expression in treatment groups increased significantly compared with the other groups. HIF- 1α, VEGF, EPO expression in the treatment control group increased significantly compared with the control group. Joint use of SCF and G-CSF increased the number of BMSCs in damaged kidney tissue and reduced the degree of renal tissue damage. BMSCs promote increased HIF-1α expression in renal tissue. Increased kidney tissue HIF- 1α and its target gene products VEGF and EPO expression possibly induce SCF and G-CSF to promote acute tubular necrosis repair.

[Thyroidectomy and injury of recurrent laryngeal nerve].

OBJECTIVE: To study factors related to recurrent laryngeal nerve (RLN) paralysis, a major complication of thyroidectomy. METHOD: Retrospective study of outcome of patient's medical records in Xijing Hospital and Tumor Hospital of Liaoning Province. Records of 1,563 patients who underwent thyroidectomy by departments of general surgery, ENT and head & neck surgery at these two hospitals were reviewed for RLN paralysis. MAIN OUTCOME MEASURES: RLN injury was analyzed in relation to types of surgery, RLN identification, and histopathology. RESULT: The incidence of RLN paralysis was 7.8%, and significantly related to the histopathologic findings of malignancy (P < 0.01). The positive identification and prevention of RLN would not be in either permanent RLN paralysis or temporary RLN paralysis, and the types of surgery were not found to be significant factors in both paralysis. CONCLUSION: RLNs should be identified to avoid iatrogenic injury and subsequent paralysis, and meticulous surgical technique should be applied in patients whose results of biopsy suggested malignancy.