RESUMEN
Several phosphaquinolinone derivatives have been synthesized and characterized to explore their usefulness in the realm of cell imaging. Solution-state photophysical properties in both aqueous and organic solutions were collected for these derivatives. Additionally, CCK-8 cell viability assays and fluorescent imaging in HeLa cells incubated with the new heterocyclic derivatives show evidence of favorable cell permeability, cell viability, and moderate intracellular localization when appended with the well-known morpholine targeting motif.
Asunto(s)
Colorantes Fluorescentes , Agua , Humanos , Estructura Molecular , Células HeLa , Ionóforos , Concentración de Iones de HidrógenoRESUMEN
We highlight a convenient synthesis to selectively deuterate an aryl C-H hydrogen bond donor in an arylethynyl bisurea supramolecular anion receptor and use the Perrin method of competitive titrations to study the deuterium equilibrium isotope effects (DEIE) of anion binding for HS-, Cl-, and Br-. This work highlights the utility and also challenges in using this method to determine EIE with highly reactive and/or weakly binding anions.