RESUMEN
In this study, we have compared the influence of CsA and pred/aza on the immunocompetence in man. Therefore, kidney-transplant recipients were tested for their primary keyhole limpet hemocyanin and secondary (tetanus and KLH) humoral immune responses and their primary dinitrochlorobenzene and secondary (recall antigens) cellular immune responses. We demonstrate that primary immune responses are inhibited by CsA, whereas secondary immune responses are relatively resistant. Pred/aza therapy seems to inhibit all cellular immune responses, as we demonstrated before, as well as the primary humoral immune responses. Secondary humoral immune responses are only slightly affected by pred/aza. Our results provide a strong argument for starting immunosuppression with CsA, either with or without a low-dose pred.
Asunto(s)
Formación de Anticuerpos/efectos de los fármacos , Azatioprina/uso terapéutico , Ciclosporinas/uso terapéutico , Inmunidad Celular/efectos de los fármacos , Trasplante de Riñón , Prednisona/uso terapéutico , Humanos , Hipersensibilidad Tardía/fisiopatologíaAsunto(s)
Formación de Anticuerpos , Ciclosporinas/uso terapéutico , Inmunidad Celular , Terapia de Inmunosupresión , Trasplante de Riñón , Linfocitos T/inmunología , Antígenos de Diferenciación de Linfocitos T/análisis , Estudios de Seguimiento , Rechazo de Injerto , Humanos , Memoria Inmunológica , Técnicas In Vitro , Recuento de Leucocitos , Activación de Linfocitos , Factores de TiempoRESUMEN
Hybridoma growth factor (HGF) is a 20-25 kD protein, supporting the growth of hybridoma cells in vitro and capable of replacing feeder cells. It was shown to be produced by human monocytes and a number of cultured cell lines. Recently, HGF was found to be identical to interferon-beta 2 or 26 kD protein and BSF-2, and was renamed interleukin 6 (IL-6). Using a sensitive bio-assay we were able to measure IL-6 activity in the serum and urine of healthy volunteers and renal transplant recipients. Low levels of IL-6 were present in the serum but not in the urine of healthy individuals. In contrast, both serum and urine of renal transplant recipients contained high levels of IL-6 directly after transplantation and during acute rejection episodes. On the basis of kinetic studies of the IL-6 response, it is concluded that serial measurement of IL-6, especially in urine, may be of value in monitoring renal transplant recipients. Moreover, the sensitivity of the bioassay will allow for detailed studies as to the biological significance of IL-6 in health and disease.