Precision finishing of South African lambs in feedlots: a review.

In the intensification of sheep production systems, feedlot finishing plays a fundamental role in preparing lambs for slaughter, as well as relieving the grazing pressure on pasture. The profit margins in feedlot operations are often narrow and require the economics of scale to generate a sufficient income. In order to minimise expenses, intensive management and precision rearing of lambs to an ideal slaughter weight is needed to obtain premium carcass prices. The South African sheep industry is made up of wool, dual-purpose as well as meat type breeds, which also vary in terms of maturity. In order to implement precision finishing of South African lamb, a complete understanding of the growth, intake and fat deposition trends of growing lambs of different breed types is needed. This review outlines feedlot lamb production within the Southern African context for the major commercial breeds, while also providing insight in the considerations necessary to develop a decision support system for lamb rearing. Integrating such a decision support system into a lamb feedlot operation can then be used for precision finishing of lambs by predicting the optimal length of the feeding period and ideal slaughter weights of lambs.

The effects of titanium dioxide coatings on light-derived heating and transdermal heat transfer in bovine skin.

The effects of titanium dioxide coatings of bovine hides on light absorption and transdermal transfer of light-derived heat were investigated. Four hair-on rug hides from Holstein cattle were purchased. Twelve samples about 20 cm on a side were cut from each hide; nine from the black-colored areas, and three from the white areas. Samples were randomized and assigned to four coating treatments: (1) white hide with no coating (White), (2) black hide with no coating (Black), (3) black hide with 50% coating (Mid), and (4) black hide with 100% coating (High). Coatings were applied to the black hide samples using a hand sprayer. Lux measurements were taken using a modified lux meter at three light intensities generated with a broad spectrum, cold halogen light source. Reflectance over a wavelength range of 380 to 900 nm was measured using a spectroradiometer. The transdermal transfer of heat derived from absorbed light was measured by applying a broad spectrum, cold halogen light source to the stratum corneum (coated) side of the sample and recording the temperature of the dermis-side using a thermal camera for 10 min at 30-s intervals. At the high light level, the White, Black, Mid, and High coating treatments had different (P < 0.001) lux values of 64,945, 1741, 15,978, and 40,730 lx, respectively. In the visible wavelength range (400 to 750 nm), Black hides reflected 10 to 15% of the light energy, hides with the Mid coating treatment reflected 35 to 40%, and hides with the High coating treatment reflected 70 to 80% of the light energy. The natural White hide samples reflected 60 to 80% of the light energy. The average maximum temperatures at the dermis-side of the hides due to transferred heat were 34.5, 70.1, 55.0, and 31.7, for the White, Black, Mid, and High treatments, respectively. Reflective coatings containing titanium dioxide on cattle hides were effective in reducing light energy absorption and reduced light-derived heat transfer from the skin surface to deeper skin layers.

Molecular and functional identification of organic anion transporter isoforms in cultured bovine mammary epithelial cells (BME-UV).

Mammary epithelial cells express a diversity of membrane transporters including members of organic cation and organic anion (OAT) transporter subfamilies. Four mammal OAT isoforms have been identified: OAT-1, OAT-2, OAT-3, and OAT-4. The pharmacological significance of OAT isoforms has been emphasized because of their role in the movement of a wide variety of substrates across epithelial barriers. The present study identified (molecularly and functionally) bovine OAT isoforms in bovine mammary epithelial (BME-UV) cells. mRNA expression levels of all tested transporters in BME-UV cells were less than expression levels of the corresponding transporters in bovine kidney. Directionality in the flux of P-aminohippuric acid and acetylsalicylate, compounds known to interact with OAT-1 and OAT-2, respectively, across BME-UV monolayers was not observed at the concentrations used in this study. Directionality was, however, observed in the flux of estrone sulfate (EsS). Adding probenecid, penicillin G or nonradiolabeled EsS to the apical donor compartment significantly increased the apical-to-basolateral flux of EsS across the BME-UV monolayer. These results suggest that BME-UV cells express an organic anion transport system, making it a potentially useful model to study the role of this transport system in the mammary epithelial barrier.

Antacids in the initial management of metallic zinc ingestion in dogs.

Zinc poisoning in dogs, following ingestion of post-1982 U.S. one cent coins is an increasingly common toxicological syndrome causing gastrointestinal abnormalities, hemolytic anemia, pancreatitis and renal failure. Thermodynamic laws predict that the rate of the chemical reaction between HCl and metallic zinc, which releases absorbable zinc anions, is dependent on pH. The significance of the relation between pH and dissolution is, however, apparently contradicted by the fact that recent veterinary toxicology texts are silent on the use of antacids in the early management of zinc ingestion in dogs. A series of experiments were conducted to test the hypotheses that the degree to which zinc dissolution is pH dependent is likely to be of clinical importance and that the use of antacids will be of benefit in preventing zinc poisoning in dogs that had ingested metallic zinc. Zinc dissolution was strongly dependent on pH in an exponential manner, indicating that clinically achievable upward adjustment of gastric pH using antacids is likely to have significant effects on the rate of zinc absorption in dogs. These data clearly support the use of antacids during the initial treatment of metallic zinc ingestion in dogs.

Cultured mammary epithelial monolayers (BME-UV) express functional organic anion and cation transporters.

There is ongoing concern about the potential adverse effects of xenobiotic residues in cows' milk to the human consumer. Although drugs that are intentionally administered to lactating dairy cattle are rigorously regulated to prevent harmful residues, there are numerous other potential sources of exposure that are not as easily controlled. For example, cattle may be exposed to mycotoxins, pesticides and/or persistent organic pollutants through feed, water and inhalation of polluted air. Accurate estimates of the rate and extent of excretion of these compounds into milk is important to assess the risk of exposure through cows' milk. In the present study, the expression of carrier mediated transport processes in cultured monolayers of an immortalized bovine mammary epithelial cell line (BME-UV) was determined using a flow-through diffusion cell system, selective substrates and inhibitors of organic cation transporters (OCT) and organic anion transporters (OAT). The basal-to-apical (BL-to-Ap) flux of tetraethylammonium and estrone sulfate significantly exceeded their flux in the opposite direction. The addition of selective inhibitors to the donor compartment significantly decreased the BL-to-Ap flux of either selective substrate. These results suggest that both OCT and OAT are functionally expressed by BME-UV cells.

In vitro anthelmintic, antibacterial and cytotoxic effects of extracts from plants used in South African ethnoveterinary medicine.

Many plants are used for ethnoveterinary purposes in South Africa, particularly in rural areas. Extracts of 17 plant species employed to treat infectious diseases were prepared using three solvents and the antibacterial activity of the extracts was determined against two Gram-positive and two Gram-negative bacteria. Anthelmintic activity was evaluated against the free-living nematode Caenorhabditis elegans and toxicity was determined using the brine shrimp larval mortality test. Most of the plant extracts demonstrated antibacterial activity, with the best minimum inhibitory concentration (MIC) being 0.1 mg mL(-1). More than a third of the extracts displayed anthelmintic activity. Toxic effects against brine shrimp larvae were shown by 30% of extracts, with the lowest LC(50) recorded as 0.6 mg mL(-1). The promising biological activity displayed by a number of plant extracts supports the ethnoveterinary use of these plants but in vivo tests are required to ascertain fully their medicinal properties and potential toxicity.

Planning a Radiotherapy Department.

The master planning of new radiotherapy facilities requires the input and engagement of a range of highly specialised professionals, both in the construction and health sector. Although radiation protection and safety aspects of radiotherapy services are universal, low and middle income countries are often presented with unique challenges that also need to be considered, e.g. competing needs within the health sector, lack of financial and human resources, environmental factors like poor provision of transport or electrical power, inadequate regulatory infrastructure, etc. Efforts to establish, upgrade or expand radiotherapy services should therefore not only focus on the technology that is appropriate and sustainable, but also be mindful of the need for quality, safety and optimal utilisation of technology. The workflow in a radiotherapy department can be facilitated by strategic placement of the main functional areas into the concept design.

Cobalt-60 Machines and Medical Linear Accelerators: Competing Technologies for External Beam Radiotherapy.

Medical linear accelerators (linacs) and cobalt-60 machines are both mature technologies for external beam radiotherapy. A comparison is made between these two technologies in terms of infrastructure and maintenance, dosimetry, shielding requirements, staffing, costs, security, patient throughput and clinical use. Infrastructure and maintenance are more demanding for linacs due to the complex electric componentry. In dosimetry, a higher beam energy, modulated dose rate and smaller focal spot size mean that it is easier to create an optimised treatment with a linac for conformal dose coverage of the tumour while sparing healthy organs at risk. In shielding, the requirements for a concrete bunker are similar for cobalt-60 machines and linacs but extra shielding and protection from neutrons are required for linacs. Staffing levels can be higher for linacs and more staff training is required for linacs. Life cycle costs are higher for linacs, especially multi-energy linacs. Security is more complex for cobalt-60 machines because of the high activity radioactive source. Patient throughput can be affected by source decay for cobalt-60 machines but poor maintenance and breakdowns can severely affect patient throughput for linacs. In clinical use, more complex treatment techniques are easier to achieve with linacs, and the availability of electron beams on high-energy linacs can be useful for certain treatments. In summary, there is no simple answer to the question of the choice of either cobalt-60 machines or linacs for radiotherapy in low- and middle-income countries. In fact a radiotherapy department with a combination of technologies, including orthovoltage X-ray units, may be an option. Local needs, conditions and resources will have to be factored into any decision on technology taking into account the characteristics of both forms of teletherapy, with the primary goal being the sustainability of the radiotherapy service over the useful lifetime of the equipment.

A physiologically based pharmacokinetic model of organophosphate dermal absorption.

The rate and extent of dermal absorption are important in the analysis of risk from dermal exposure to toxic chemicals and for the development of topically applied drugs, barriers, insect repellents, and cosmetics. In vitro flow-through cells offer a convenient method for the study of dermal absorption that is relevant to the initial processes of dermal absorption. This study describes a physiologically based pharmacokinetic (PBPK) model developed to simulate the absorption of organophosphate pesticides, such as parathion, fenthion, and methyl parathion through porcine skin with flow-through cells. Parameters related to the structure of the stratum corneum and solvent evaporation rates were independently estimated. Three parameters were optimized based on experimental dermal absorption data, including solvent evaporation rate, diffusivity, and a mass transfer factor. Diffusion cell studies were conducted to validate the model under a variety of conditions, including different dose ranges (6.3-106.9 microg/cm2 for parathion; 0.8-23.6 microg/cm2 for fenthion; 1.6-39.3 microg/cm2 for methyl parathion), different solvents (ethanol, 2-propanol and acetone), different solvent volumes (5-120 microl for ethanol; 20-80 microl for 2-propanol and acetone), occlusion versus open to atmosphere dosing, and corneocyte removal by tape-stripping. The study demonstrated the utility of PBPK models for studying dermal absorption, which can be useful as explanatory and predictive tools that may be used for in silico hypotheses generation and limited hypotheses testing. The similarity between the overall shapes of the experimental and model-predicted flux/time curves and the successful simulation of altered system conditions for this series of small, lipophilic compounds indicated that the absorption processes that were described in the model successfully simulated important aspects of dermal absorption in flow-through cells. These data have direct relevance to topical organophosphate pesticide risk assessments.

Ethnoveterinary medicine practices among Tsonga speaking people of South Africa.

Rapid Rural Appraisal methods were used to collate and code the indigenous knowledge on animal healthcare of Tsonga speaking people of South Africa. There was a rapport between local disease names as described by their clinical signs by the farmers and the local veterinary services important disease list. The perceived causes of diseases were physico-biological elements and no reference to ancestral guidance was recorded. Males and old people were more knowledgeable but females and young people did show a certain degree of confidence during general discussions. Plants were more frequently used than other non-conventional remedies with cattle being the most treated animals. Farmers reported using 19 plant species belonging to 12 families. Plants were collected from the wild when needed and no specific storage system was used. They were administered as decoctions or infusions of single plants. These remedies were used not only as alternatives to expensive pharmaceutical products but also because in certain diseases or chronic cases, they were thought to be more efficacious.

Microscopic morphology of Dichapetalum cymosum (Hook.) Engl. as an aid in the identification of leaf fragments from the digestive tract of poisoned animals.

Dichapetalum cymosum (Hook.) Engl. (Poison leaf; gifblaar) is a major cause of acute livestock plant poisoning in southern Africa. Microscopic identification of leaf fragments found in the digestive tract of poisoned animals can assist in the diagnosis of poisoning when D. cymosum poisoning is suspected. The microscopic morphology of D. cymosum leaves are described using standard staining and microscopy methods for histopathology samples at many regional diagnostic laboratories. Morphological descriptions included structures in the epidermis and mesophyll that were discernible using H & E staining. The microscopic morphology of D. cymosum was used to differentiate between leaf fragments of D. cymosum and other species from the same habitat with macroscopic features that resemble those of D. cymosum, including Euclea crispa, Combretum zeyheri, Burkea afrikana and Lannea discolor.

Comparative studies on the effects of water, ethanol and water/ethanol mixtures on chemical partitioning into porcine stratum corneum and silastic membrane.

The effects of water and ethanol vehicles on stratum corneum and silastic membrane partitioning of 11 industrial and agricultural compounds were studied to aid in characterizing and assessing risk from skin exposure. Zero percent, 50% and 100% aqueous ethanol solutions were used as solvents for (14)C labeled phenol, 4-nitrophenol, pentachlorophenol, dimethyl parathion, parathion, chloropyrifos, fenthion, triazine, atrazine, simazine and propazine. Compound partitioning between the solvents and porcine stratum corneum/silastic membrane were estimated. Stratum corneum was exposed to aqueous ethanol ranging from 0% to 100% v/v ethanol in 20% increments and Fourier transform infrared spectroscopy (FT-IR) was used to obtain an index of lipid disorder. Gravimetry and FT-IR were used to demonstrate lipid extraction in aqueous ethanol solutions. Partitioning patterns in silastic membranes resembled those in stratum corneum and were correlated with octanol/water partitioning. Partitioning was highest in water and was higher from 50% ethanol than from 100% ethanol, except for parathion, 4-nitrophenol, atrazine and propazine. Correlation existed between molecular weight and partitioning in water, but not in ethanol and ethanol/water mixtures. Lipid order, as reflected in FT-IR spectra, was not altered. These studies suggest that stratum corneum partitioning of the compounds tested is primarily determined by relative compound solubility between the stratum corneum lipids and the donor solvent. Linear relationships existed between octanol/water partitioning and stratum corneum partitioning. Partitioning was also correlated with molecular weight in water solvent systems, but not in ethanol and ethanol/water mixtures. Ethanol and ethanol/water mixtures altered the stratum corneum through lipid extraction, rather than through disruption of lipid order.

Total skin electron therapy: a technique which can be implemented on a conventional electron linear accelerator.

PURPOSE: A technique for treating whole-body skin with an electron linear accelerator with nominal energies in the range 4-8 MeV is presented. Stationary fields at an extended source-skin distance are used with the patient treated in a reclined position. METHODS AND MATERIALS: The relative beam data, absolute dosimetry measurements and the patient setup parameters are presented. The calculations required to correct for patient size are discussed. RESULTS: The technique described uses a six field circumferential cycle, with longitudinally matched fields along the length of the patient. Treatment times are reasonable using the standard dose rate of the machine. The uniformity of the skin dose measured on three patients was found to be comparable to that of other total skin treatment techniques. CONCLUSION: A technique for treating conditions like mycosis fungoides is presented requiring relatively simple supporting dosimetry. No modifications to the unit are required and no sophisticated treatment apparatus is necessary, which makes the technique attractive to smaller Institutions, especially in developing countries, where technical support may be limited.

Management of depressed skull fractures. Selective conservative management of nonmissile injuries.

The management of 319 cases with nonmissible depressed skull fractures is discussed. The majority of these injuries (75%) resulted from assaults and more than 90% were compound fractures. Of these, 35 cases were excluded from the series; these were patients who died before investigation or treatment could be instituted, or whose major injury was an intracranial hematoma or extensive traumatic hemorrhagic necrosis rather than a depressed fracture with underlying localized contusion. In the remaining 284 cases a comparison is made between the outcome in 124 (44%) patients treated by a conventional surgical method and 160 (56%) selected patients whose wound was only inspected, cleaned, and sutured in the emergency room. The nonsurgical group included 21 (9%) patients with simple depressed fractures and also patients without severe wound contamination, established infection, severe comminution of the fracture, or brain or cerebrospinal fluid in the wound. The results of management were assessed in relation to septic complications, the outcome of focal neurological abnormalities, and fatal complications. The group treated conservatively compared favorably with the surgical cases and also with previously reported series. The obvious bias in favor of the conservative group is admitted; however, it is clear that the majority of simple fractures and some compound depressed skull fractures can be managed safely without major surgical intervention. Socioeconomic implications are discussed.

The use of a diffusion test for the detection of antibiotics in the tissues of slaughter stock.

The Brilliant Black Reduction Test Kit (BR Test), which is widely used to detect antimicrobial residues in milk, was adapted to detect residues in the meat and tissues of slaughter stock. The adaptation consisted of placing kidney and muscle tissue samples into 2.5ml diffusion cups containing 0.4ml media plus Bacillus stearothermophilus spores and brilliant black indicator. A preliminary trial undertaken to test the lower limits of sensitivity of the adapted BR Test to a number of the more common antibiotics used in food animals, was followed by a survey involving 943 pigs slaughtered at one abattoir. Samples were tested from 87 suppliers of which 11 regularly marketed pigs with detectable antimicrobial residues. Most of these pigs came from large pig producing units. Three suppliers marketed pigs with suspicious reactions. No residues could be detected in pigs from the remaining 73 suppliers. The BR Test was found to be a quick, inexpensive, practical screening test which could be utilized for the routine detection of antimicrobial residues in slaughter stock at all South African abattoirs.

Use of ethnoveterinary medicinal plants in cattle by Setswana-speaking people in the Madikwe area of the North West Province of South Africa.

Rapid Rural Appraisal (RRA) methods were employed to document the use of ethnoveterinary medicinal plants in cattle by Setswana-speaking people in the Madikwe area of the North West Province of South Africa. The study indicated that Setswana-speaking people in the North West Province have a rich heritage of ethnoveterinary knowledge, which includes all aspects of ethnoveterinary medicinal plant use. Information was gathered from informants through individual interviews, group interviews, guided field walks and observations. Ethnoveterinary uses in cattle of 45 plant species representing 24 families were recorded. Plants were used in 84% of the total number of recorded ethnoveterinary remedies. These plants were used alone (64%) or in mixtures (36%) for 29 indications. The most important indications were retained placenta, diarrhoea, gallsickness, fractures, eye inflammation, general ailments, fertility enhancement, general gastrointestinal problems, heartwater, internal parasites, coughing, redwater and reduction of tick burden. Plant materials were prepared in various ways including infusion, decoction, ground fresh material, sap expressed from fresh material, charred and dried. The most common dosage form was a liquid for oral dosing. Other dosage forms included drops, licks, ointments, lotions and powders. Liquid remedies for oral dosing were always administered using a bottle. Medicinal plant material was preferably stored in a dried form in a cool place out of direct sunlight and wind. Lack of transfer of ethnoveterinary knowledge to younger generations puts this knowledge at risk. RRA was found to be a successful method of investigation for the study of ethnoveterinary medicine.

Evaluation of a commercially available molybdate formulation and zinc oxide boluses in preventing hepatic copper accumulation and thus enzootic icterus in sheep.

The efficacy of a molybdate formulation and a zinc oxide bolus as prophylactic agents for enzootic icterus was evaluated in sheep. Before copper loading, liver biopsies were performed on 12 male, 6-month-old, Mutton Merino sheep to determine hepatic copper (Cu) and zinc (Zn) concentrations. The animals were restrictively randomised according to liver copper concentrations to 3 treatment groups (n = 4) to achieve similar mean liver copper concentrations per group. All sheep received 4 ml/kg of a 0.5 % aqueous solution of CuSO4 5H2O intraruminally 7 days per week for 10 weeks. On Day 0 the sheep in the Mo-group were injected subcutaneously with 42 mg molybdenum (Mo) contained in a commercial molybdate formulation. The animals in the Zn-group each received a zinc oxide bolus, containing 43 g zinc oxide, via a rumen cannula. Treatment was repeated on Day 42. Four animals served as untreated controls. Urinary copper excretion, plasma copper concentration, haematocrit and glutamate dehydrogenase (GLDH) activity were determined throughout the trial. The animals were sacrificed after 10 weeks and liver samples were submitted for histopathological examination. Liver and kidney copper and zinc concentrations were determined. Neither the molybdate treatment nor the zinc oxide boluses prevented hepatic copper accumulation. The urinary copper excretion, plasma copper concentration, haematocrit and GLDH activity were not significantly different (P > 0.05) from the controls.

Evaluations of land cover risk factors for canine leptospirosis: 94 cases (2002-2009).

Associations of land cover/land use variables and the presence of dogs in urban vs. rural address locations were evaluated retrospectively as potential risk factors for canine leptospirosis in Kansas and Nebraska using Geographic Information Systems (GIS). The sample included 94 dogs positive for leptospirosis predominantly based on a positive polymerase chain reaction test for leptospires in urine, isolation of leptospires on urine culture, a single reciprocal serum titer of 12,800 or greater, or a four-fold rise in reciprocal serum titers over a 2-4 weeks period; and 185 dogs negative for leptospirosis based on a negative polymerase chain reaction test and reciprocal serum titers less than 400. Land cover features from 2001 National Land Cover Dataset and 2001 Kansas Gap Analysis Program datasets around geocoded addresses of case/control locations were extracted using 2500m buffers, and the presence of dogs' address locations within urban vs. rural areas were estimated in GIS. Multivariate logistic models were used to determine the risk of different land cover variables and address locations to dogs. Medium intensity urban areas (OR=1.805, 95% C.I.=1.396, 2.334), urban areas in general (OR=2.021, 95% C.I.=1.360, 3.003), and having urban address locations (OR=3.732, 95% C.I.=1.935, 7.196 entire study region), were significant risk factors for canine leptospirosis. Dogs regardless of age, sex and breed that live in urban areas are at higher risk of leptospirosis and vaccination should be considered.