Non-thermal atmospheric pressure gas discharge plasma enhances tendon-to-bone junction repair in a rabbit model.

BACKGROUND: Plasma is a collection of active particles generated by dissociating molecules and ionizing atoms through applying high energy to a gas, such as high-sound heating or electrical shock. Recently, many reports have been published on the effectiveness of non-thermal atmospheric pressure gas discharge plasma (NTAPP) on living organisms. Furthermore, we have reported on the promotion of bone and tendon repair by NTAPP irradiation. We hypothesized that irradiation of NTAPP would promote the repair of the tendon-bone junction in a rotator cuff repair. This study investigated the effect of NTAPP irradiation on the healing process of the tendon-bone junction. METHODS: Among 36 Japanese white rabbits, the infraspinatus tendon was detached from the humeral insertion site. A 3.2 mm bone tunnel was then created at the original insertion site of the infraspinatus muscle. The left shoulder was irradiated with NTAPP at a distance of 1 cm from the bone tunnel for 5 minutes (plasma-treated group), while the right shoulder was not irradiated (control group). The rabbits were sacrificed at 2, 4, and 8 weeks postoperatively, and six of each were used for histological evaluation. Mechanical tests were also performed on six specimens each at 4 and 8 weeks. RESULTS: Histological evaluation showed that at 4 weeks, the histological tendon to bone maturing score was 6.8±1.3 in the plasma-treated group and 4.8±1.6 in the control group (p<0.01); at 8 weeks it was 9.0±1.0 in the plasma-treated group and 5.2±1.1 in the control group (p<0.01). Fibrocartilage formation and new bone formation were observed at both 4 and 8 weeks. In the mechanical test, the plasma-treated group had 75.0 ± 18.9 N in ultimate load to failure at 8 weeks. In the control group, it was 51.1±7.9 N. (p=0.04) CONCLUSION: The repair of the rotator cuff at the tendon-bone junction was significantly improved at 4 and 8 weeks by irradiation with NTAPP.

Developing Novel Gas Discharge Emitters of Acoustic Waves in Air for Nondestructive Testing of Materials.

This study was devoted to the development of novel devices and a methodology intended for generating ultrasonic waves in an air medium by using atmospheric pressure gas discharge. In the proposed electrode system, the discharge process was accompanied by the generation of acoustic waves on the emitter surface and, consequently, in the ambient air. The gas discharge emitter vibrations were analyzed by applying the technique of Scanning Laser Doppler Vibrometry (SLDV). It was shown that the magnitude of displacements matched the corresponding characteristics of classical piezoelectric and magnetostrictive transducers. The use of the Fast Fourier transform procedure supplied amplitude-frequency spectra of vibrations generated by the gas discharge emitter. The amplitude-frequency spectrum analysis showed that the proposed emitter was able to generate acoustic waves in the air with frequencies from 50 Hz to 100 kHz, and such a device can be used for the nondestructive testing (NDT) of materials. The results of the statistical analysis of vibration displacements in the repetitive pulsed mode were discussed. A non-stable characteristic of the vibration displacement of the emitter membrane was demonstrated. The parameters of such instability were associated with the features of gas discharge processes. In the experiments, the proposed gas discharge emitter was used in combination with SLDV for inspecting carbon-fiber-reinforced polymer composites. The experiments demonstrated the possibility of using an air-coupled gas discharge transmitter to generate acoustic waves in NDT applications.

Effect of Negative Ion Generation on Complex Plasma Structure Properties.

We propose a low-density discharge plasma model that takes into account the impact of oxygen admixture in typical conditions of complex (dusty) plasmas. Numerical simulations based on this model show that the concentration of negative ions turns out to be very high, and they play an important role in the overall kinetics in this particular range of plasma conditions. The ambipolar diffusion electric field drags these negative ions into the center of the plasma. The density of negative ions is high enough to push the negatively charged dust component out of the center, both by weakening the radial electric field and by increasing the thermophoretic force. This phenomenon was observed in the published experiment and qualitatively supports the proposed model. Additionally, the proposed model allows an alternative explanation of the experiment.

Modelling of carbon nanotube film based temperature sensor: thermal emission and gas discharge.

The carbon nanotube (CNT) film based ionized temperature sensor is sensitive to gas temperature, and shows good sensitivity compared with other temperature sensors. But it is still unclear about the effect of CNT film on thermal emission and gas discharge at different temperatures. In this article, we established a gas discharge model of the CNT film temperature sensor. Field assisted thermal emission is simulated at the tip of CNTs by analysing the field enhancement effect and effective work function. Ionization collision, excitation, recombination collision, Penning ionization and quenching of argon are considered in order to obtain the interaction of various particles at different temperature. The current density-temperature characteristic of the temperature sensor was obtained at 24-80 V. The increase of the working voltage is helpful to improve the output current and sensitivity of the temperature sensor. Response time of the sensor will not change in the temperature range of 293-373 K. However, the change of temperature will affect the current density, secondary electron emission and reaction rate. In addition, the sensor has different temperature sensitivity in argon and helium. The above simulation results are helpful to understand the role of CNT film and temperature sensitivity of the ionized sensor. It can also be used to study and improve the sensitivity of this type of sensor.

Approaches to Inactivating Aflatoxins-A Review and Challenges.

According to the World Health Organization, the contamination of crops with aflatoxins poses a significant economic burden, estimated to affect 25% of global food crops. In the event that the contaminated food is processed, aflatoxins enter the general food supply and can cause serious diseases. Aflatoxins are distributed unevenly in food or feedstock, making eradicating them both a scientific and a technological challenge. Cooking, freezing, or pressurizing have little effect on aflatoxins. While chemical methods degrade toxins on the surface of contaminated food, the destruction inside entails a slow process. Physical techniques, such as irradiation with ultraviolet photons, pulses of extensive white radiation, and gaseous plasma, are promising; yet, the exact mechanisms concerning how these techniques degrade aflatoxins require further study. Correlations between the efficiency of such degradation and the processing parameters used by various authors are presented in this review. The lack of appropriate guidance while interpreting the observed results is a huge scientific challenge.

Alignments of a Microparticle Pair in a Glow Discharge.

Stability of a vertically aligned microparticle pair in a stratified glow DC discharge is experimentally investigated. Using laser perturbations, it is shown that, for the same discharge parameters, a pair of microparticles can be suspended in two stable configurations: vertical and horizontal. The interparticle interaction and the electric field of the stratum in the region of particle levitation are quantitatively investigated for the first time. The decharging effect of the lower (downstream) particle by the ion flow wake is also observed for the first time in a glow discharge. The obtained experimental data made it possible to check the analytical criteria for the configurational stability of the system.

Optimising waste from electric and electronic equipment collection systems: a comparison of approaches in European countries.

The first European waste from electric and electronic equipment directive obliged the Member States to collect 4 kg of used devices per inhabitant and year. The target of the amended directive focuses on the ratio between the amount of waste from electric and electronic equipment collected and the mass of electric and electronic devices put on the market in the three foregoing years. The minimum collection target is 45% starting in 2016, being increased to 65% in 2019 or alternatively 85% of waste from electric and electronic equipment generated. Being aware of the new target, the question arises how Member States with 'best practice' organise their collection systems and how they enforce the parties in this playing field. Therefore the waste from electric and electronic equipment schemes of Sweden, Denmark, Switzerland, Germany and the Flemish region of Belgium were investigated focusing on the categories IT and telecommunications equipment, consumer equipment like audio systems and discharge lamps containing hazardous substances, e.g. mercury. The systems for waste from electric and electronic equipment collection in these countries vary considerably. Recycling yards turned out to be the backbone of waste from electric and electronic equipment collection in most countries studied. For discharge lamps, take-back by retailers seems to be more important. Sampling points like special containers in shopping centres, lidded waste bins and complementary return of used devices in all retail shops for electric equipment may serve as supplements. High transparency of collection and recycling efforts can encourage ambition among the concerned parties. Though the results from the study cannot be transferred in a simplistic manner, they serve as an indication for best practice methods for waste from electric and electronic equipment collection.

Advances in atmospheric pressure plasma-based optical emission spectrometry for the analysis of heavy metals.

Over the past decade, miniaturized optical emission spectrometry (OES) systems utilizing atmospheric pressure plasmas (APPs) as radiation sources have exhibited impressive capabilities in trace heavy metal analysis. As the core of the analytical system, APPs sources possess unique properties such as compact size, light weight, low energy requirement, ease of fabrication, and relatively low manufacturing cost. This critical review focuses on recent progress of APP-based OES systems employed for the determination of heavy metals. Influences of technical details including the sample introduction manner, the sampling volume, the sample flow rate, the pH of the solutions on the plasma stability and the intensity of analytical signals are comprehensively discussed. Furthermore, the review emphasizes the analytical challenges faced by these techniques and highlights the opportunities for further development in the field of heavy metal detection.

Low power gas discharge plasma mediated inactivation and removal of biofilms formed on biomaterials.

The antibacterial activity of gas discharge plasma has been studied for quiet some time. However, high biofilm inactivation activity of plasma was only recently reported. Studies indicate that the etching effect associated with plasmas generated represent an undesired effect, which may cause live bacteria relocation and thus contamination spreading. Meanwhile, the strong etching effects from these high power plasmas may also alter the surface chemistry and affect the biocompatibility of biomaterials. In this study, we examined the efficiency and effectiveness of low power gas discharge plasma for biofilm inactivation and removal. Among the three tested gases, oxygen, nitrogen, and argon, discharge oxygen demonstrated the best anti-biofilm activity because of its excellent ability in killing bacteria in biofilms and mild etching effects. Low power discharge oxygen completely killed and then removed the dead bacteria from attached surface but had negligible effects on the biocompatibility of materials. DNA left on the regenerated surface after removal of biofilms did not have any negative impact on tissue cell growth. On the contrary, dramatically increased growth was found for these cells seeded on regenerated surfaces. These results demonstrate the potential applications of low power discharge oxygen in biofilm treatments of biomaterials and indwelling device decontaminations.

Modeling and Characterization of Surface Discharges in Insulating Material for Spacers: Electrode Shape, Discharge Mode, and Revision of the Creepage Concept.

In the design of MV AC and DC spacers, the predominant factors are surface and interface conditions. Design is generally carried out on specifications and standards which are based on long-term experience and lab testing. However, the diffusion of power electronics with a trend to increase electric field, switching frequency, and rise time to achieve higher power density calls for an innovative, global approach to optimized insulation system design. A new methodology, based on field simulation, discharge modeling, and partial discharge inception measurements, called the three-leg approach, can form the basis to optimize insulation design for any type of supply voltage waveform. This paper focuses on the influence of the type of electrode on the inception and phenomenology of surface discharges and, as a consequence, on the interpretation of the results used for application of the three-leg approach. It is demonstrated that a typical electrode system used for insulating material testing can generate both gas and surface discharges at the triple point, when the electrodes have a smooth profile that is used to avoid corona or flashover. Hence, testing partial discharge may not provide a straightforward indication of the surface discharge inception and, thus, be partially misleading for insulation design. Another takeover is that such analysis must benefit from PD testing tools endowed with analytics able to provide automatic identification of the type of defect generating PD, i.e., internal, surface, and corona, since design and remedy actions can be taken, and adequate insulating materials developed, only knowing the type of source generating PD. Hence, testing partial discharge may not provide a straightforward indication of surface discharge inception and, thus, be partially misleading for insulation design. In addition to the importance of the three-leg approach to favor reliable and optimized design of insulation systems, there is a clear need to have a PD testing tool endowed with analytics. It should preferably be able to provide automatic identification of the type of defect generating PD, i.e., internal, surface, and corona.

Hybrid Plasmas for Materials Processing.

Hybrid plasmas have been reported in various areas of research over the last 40 years. However, a general overview of hybrid plasmas has never been presented or reported. In the present work, a survey of the literature and patents is carried out to provide the reader with a broad view of hybrid plasmas. The term refers to several different configurations of plasmas, including but not limited to: plasmas driven by several power sources simultaneously or sequentially, plasmas that have the properties of both thermal and nonthermal plasmas, plasmas that are enhanced by additional energy, and plasmas that are operated in a unique medium. In addition, a way of evaluating hybrid plasmas in terms of the improvement of processes is discussed, as well as the negative impacts that follow the employment of hybrid plasmas. Regardless of what the hybrid plasma in question is composed of, it often poses a unique advantage to its nonhybrid counterpart, whether it be used for welding, surface treatment, materials synthesis, coating deposition, gas phase reactions, or medicine.

Claims of priority - The scientific path to the discovery of X-rays.

Shortly after Röntgen's publication about a new kind of rays, a dispute about priority claims began. Röntgen was not the first researcher to produce X-rays nor the first to take X-ray images. An analysis of the history of cathode ray research in the 19th century reveals ample evidence that researchers before Röntgen had already produced X-rays, albeit without knowing this. Most of them, for their part, did not claim any priority, some did so rather casually. The German-Hungarian physicist Philipp Lenard, a co-founder of German Physics, considered himself a "true discoverer". It remains to be said, however, that he, like many others before him, failed to recognize the character of the new radiation. It was Wilhelm Conrad Röntgen, with his three scientific publications on X-rays, who laid the foundations for their physical clarification and paved the way for the success story of their application in a variety of fields that continues to this day.

Plasma depolymerization of chitosan in the presence of hydrogen peroxide.

The depolymerization of chitosan by plasma in the presence of hydrogen peroxide (H(2)O(2)) was investigated. The efficiency of the depolymerization was demonstrated by means of determination of viscosity-average molecular weight and gel permeation chromatography (GPC). The structure of the depolymerized chitosan was characterized by Fourier-transform infrared spectra (FT-IR), ultraviolet spectra (UV) and X-ray diffraction (XRD). The results showed that chitosan can be effectively degradated by plasma in the presence of H(2)O(2). The chemical structure of the depolymerized chitosan was not obviously modified. The combined plasma/H(2)O(2) method is significantly efficient for scale-up manufacturing of low molecular weight chitosan.

A study of anode area physical parameters of asymmetric combined gas discharge.

The layered structure properties of an asymmetric combined gas discharge have been studied. The main physical parameters of the plasma in the zone of electron acceleration to high energies of tens and hundreds of electron-volts at various values of the supplied microwave power were determined based on the analysis of the discharge current-voltage characteristics. The effect of combined discharge plasma on the surface of products made of various materials and placed in the resonator chamber of a technological unit was experimentally investigated, and it is shown that it can lead to a significant increase in the strength of the processed products in terms of microhardness.

Microbial driven reduction of N2O and NH3 emissions during composting: Effects of bamboo charcoal and bamboo vinegar.

In this study, we systematically analyzed the microbial-driven effects of bamboo charcoal (BC) and bamboo vinegar (BV) on reducing NH3 and N2O emissions during aerobic composting. The results showed that BC and BV improved the nitrogen conversion and compost quality, but the combined BC + BV treatment obtained the best improvements. The BC, BV, and BC + BV treatments reduced the NH3 emissions by 14.35%, 17.90%, and 29.83%, respectively, and the N2O emissions by 44.83%, 55.96%, and 74.53%. BC and BV reduced the NH3 and N2O emissions during composting by controlling ammonia oxidation, where napA, nirK, and nosZ served as useful indicators of the N2O emissions from compost, especially the nirK gene. The dominant nitrifying and denitrifying bacteria belonged to Proteobacteria, and the changes in environmental factors during composting significantly affected the succession of the nitrifying and denitrifying bacterial communities. Nitrosomonas was a key nitrifying bacterial genus in the mesophilic composting period, and BC and BV may have reduced the NH3 emissions by enhancing its conversion to NH4+-N by Nitrosomonas. In addition, norank_p__environmental_samples, unclassified_k__norank_d__Bacteria, and unclassified_p__Proteobacteria were jointly responsible for driving the production of N2O during the compost maturity stage.

Acute effects of mobile phone radiations on subtle energy levels of teenagers using electrophotonic imaging technique: A randomized controlled study.

BACKGROUND: Mobile phones induce radio frequency electromagnetic field (RF-EMF) which has been found to affect subtle energy levels of adults through Electrophotonic Imaging (EPI) technique in a previous pilot study. MATERIALS AND METHODS: We enrolled 61 healthy right-handed healthy teenagers (22 males and 39 females) in the age range of 17.40 ± 0.24 years from educational institutes in Bengaluru. Subjects were randomly divided into two groups: (1) (mobile phone in ON mode [MPON] at right ear) and (2) mobile phone in OFF mode (MPOF). Subtle energy levels of various organs of the subjects were measured using gas discharge visualization Camera Pro device, in double-blind conditions, at two points of time: (1) baseline and (2) after 15 min of MPON/MPOF exposure. As the data were found normally distributed, paired and independent samples t-test were applied to perform within and between group comparisons, respectively. RESULTS: The subtle energy levels were significantly reduced after RF-EMF exposure in MPON group as compared to MPOF group for following areas: (a) Pancreas (P = 0.001), (b) thyroid gland (P = 0.002), (c) cerebral cortex (P < 0.01), (d) cerebral vessels (P < 0.05), (e) hypophysis (P = 0.013), (f) left ear and left eye (P < 0.01), (g) liver (P < 0.05), (h) right kidney (P < 0.05), (i) spleen (P < 0.04), and (j) immune system (P < 0.02). CONCLUSION: Fifteen minutes of RF-EMF exposure exerted quantifiable effects on subtle energy levels of endocrine glands, nervous system, liver, kidney, spleen, and immune system of healthy teenagers. Future studies should try to correlate these findings with respective biochemical markers and standard radio-imaging techniques.

Development of normative data of electro photonic imaging technique for healthy population in India: A normative study.

BACKGROUND: Electro photonic imaging (EPI) technique is growing as a novel technique of health assessment and is being utilized in the fields of alternative medicine, conventional practices, psycho-physiology, psychology, and consciousness studies. The existing EPI norms are based mostly on European (EU) population. In order to enhance the practice and research through EPI in India, there is a need for developing norms for the healthy Indian population. OBJECTIVE: The objective of the study was to establish the normative data of EPI for the healthy Indian population, to aid in the accuracy of EPI measurements and interpretations. MATERIALS AND METHODS: A total of 1297 volunteers were assessed once, who represented different parts of India during December 2013 to December 2014. Among them, 880 volunteers were reported to be healthy (age mean ± standard deviation [SD], 33.55 ± 10.92), with 584 males (age mean ± SD, 33.54 ± 10.86) and 296 females (age mean ± SD, 33.56 ± 11.00). In this study activation coefficient (stress level), integral area (IA) (general health), and integral entropy (disorderliness in energy) parameters were analyzed. RESULTS: As the data were not normally distributed, quartile based statistics was used for setting the norms. The 25(th) and 75(th) percentiles were calculated and they were further verified using a bootstrap procedure. Uniquely, the results showed a clear difference in IA parameters under both with filter (physiological) and without filter (psycho-physiological) conditions between the Indian and the EU population. Though other parameters were found almost similar to the EU population, inter quartile ranges were narrower in the Indian population as compared to the EU values. Similar trends were observed in the subgroup analyzes: That is, male versus female genders and age ranges 18-40 versus 40-60. CONCLUSION: As compared to EU population, Indian population had different range of Integral Area values and narrower range for values of other variables. EPI Studies in India should also adjust for factors such as age and gender.

Effect of anapanasati meditation technique through electrophotonic imaging parameters: A pilot study.

BACKGROUND: Mindfulness along with breathing is a well-established meditation technique. Breathing is an exquisite tool for exploring subtle awareness of mind and life itself. AIM: This study aimed at measuring changes in the different parameters of electrophotonic imaging (EPI) in anapanasati meditators. MATERIALS AND METHODS: To carry out this study, 51 subjects comprising 32 males and 19 females of age 18 years and above (mean age 45.64 ± 14.43) were recruited voluntarily with informed consent attending Karnataka Dhyana Mahachakra-1 at Pyramid Valley International, Bengaluru, India. The design was a single group pre- post and data collected by EPI device before and after 5 days of intensive meditation. RESULTS: Results show significant changes in EPI parameter integral area with filter (physiological) in both right and left side, which reflects the availability of high functional energy reserve in meditators. The researchers observed similar trends without filter (psycho-physiological) indicating high reserves of energy at psycho-physiological level also. Activation coefficient, another parameter of EPI, reduced showing more relaxed state than earlier, possibly due to parasympathetic dominance. Integral entropy decreased in the case of psycho-physiological parameters left-side without filter, which indicates less disorder after meditation, but these changes were not significant. The study showed a reversed change in integral entropy in the right side without filter; however, the values on both sides with filter increased, which indicates disorder. CONCLUSION: The study suggests that EPI can be used in the recording functional physiological and psychophysiological status of meditators at a subtle level.

Disinfection and toxicological assessments of pulsed UV and pulsed-plasma gas-discharge treated-water containing the waterborne protozoan enteroparasite Cryptosporidium parvum.

We report for the first time on the comparative use of pulsed-plasma gas-discharge (PPGD) and pulsed UV light (PUV) for the novel destruction of the waterborne enteroparasite Cryptosporidium parvum. It also describes the first cyto-, geno- and ecotoxicological assays undertaken to assess the safety of water decontaminated using PPGD and PUV. During PPGD treatments, the application of high voltage pulses (16 kV, 10 pps) to gas-injected water (N2 or O2, flow rate 2.5L/min) resulted in the formation of a plasma that generated free radicals, ultraviolet light, acoustic shock waves and electric fields that killed ca. 4 log C. parvum oocysts in 32 min exposure. Findings showed that PPGD-treated water produced significant cytotoxic properties (as determined by MTT and neutral red assays), genotoxic properties (as determined by comet and Ames assays), and ecotoxic properties (as determined by Microtox™, Thamnotox™ and Daphnotox™ assays) that are representative of different trophic levels in aquatic environment (p<0.05). Depending in part on the type of injected gas used, PPGD-treated water became either alkaline (pH ≤ 8.58, using O2) or acidic (pH ≥ 3.21, using N2) and contained varying levels of reactive free radicals such as ozone (0.8 mg/L) and/or dissociated nitric and nitrous acid that contributed to the observed disinfection and toxicity. Chemical analysis of PPGD-treated water revealed increasing levels of electrode metals that were present at ≤ 30 times the tolerated respective values for EU drinking water. PUV-treated water did not exhibit any toxicity and was shown to be far superior to that of PPGD for killing C. parvum oocysts taking only 90 s of pulsing [UV dose of 6.29 µJ/cm(2)] to produce a 4-log reduction compared to a similar reduction level achieved after 32min PPGD treatment as determined by combined in vitro CaCo-2 cell culture-qPCR.

Express-evaluation of the psycho-physiological condition of Paralympic athletes.

OBJECTIVE: Evaluation of elite athletes' psycho-physiological condition at various stages of preparation and in international competition. DESIGN: Athletes were tested during training and participation in international competition using methods of galvanic skin response (GSR) and gas discharge visualization (GDV). SETTING: Saint Petersburg Federal Research Institute of Physical Culture and Sport, Russia and Paralympic athletic training camp, Norway. PARTICIPANTS: Eighteen athletes from Russia's Skiing and Biathlon Paralympic Team. All athletes had some level of damage to their musculoskeletal system. MAIN OUTCOME MEASURES: Stress level (SL), energy potential (EP), and psycho-emotional tension (PET). RESULTS: It was found that the higher the level of EP achieved by the athlete in the training period, the lower the SL in the competition time. The SL of an athlete recorded in the training period significantly correlates with the SL both before and at the time of competition. The PET and SL before the World Cup was negatively correlated to the results of skiing competitions. CONCLUSION: Evaluation of PET, EP, and SL through GSR and GDV offers a fast, highly precise, non-invasive method to assess an athlete's level of readiness during both training and at the time of competition.