Long-Distance Cooperative and Antagonistic RNA Polymerase Dynamics via DNA Supercoiling.

Genes are often transcribed by multiple RNA polymerases (RNAPs) at densities that can vary widely across genes and environmental conditions. Here, we provide in vitro and in vivo evidence for a built-in mechanism by which co-transcribing RNAPs display either collaborative or antagonistic dynamics over long distances (>2 kb) through transcription-induced DNA supercoiling. In Escherichia coli, when the promoter is active, co-transcribing RNAPs translocate faster than a single RNAP, but their average speed is not altered by large variations in promoter strength and thus RNAP density. Environmentally induced promoter repression reduces the elongation efficiency of already-loaded RNAPs, causing premature termination and quick synthesis arrest of no-longer-needed proteins. This negative effect appears independent of RNAP convoy formation and is abrogated by topoisomerase I activity. Antagonistic dynamics can also occur between RNAPs from divergently transcribed gene pairs. Our findings may be broadly applicable given that transcription on topologically constrained DNA is the norm across organisms.

Power Dynamics Perpetuate DEI Inaction: A Qualitative Study of Community Health Clinic Teams.

PURPOSE: Despite increased clinician awareness of systemic racism, lack of substantial action toward antiracism exists within health care. Clinical staff perspectives, particularly those of racial-ethnic minorities/persons of color (POC) who disproportionately occupy support staff roles with less power on the team, can yield insights into barriers to progress and can inform future efforts to advance diversity, equity, and inclusion (DEI, also referred to as EDI) within health care settings. This qualitative study explored the perspectives of staff members on race and role power dynamics within community health clinic teams. METHODS: We conducted semistructured 45-minute interviews with staff members working in community health clinics in a large urban health care system from May to July 2021. We implemented purposeful recruitment to oversample POC and support staff and to achieve equal representation from the 13 community health clinics in the system. Interviews were audio recorded, transcribed, and analyzed over 6 months using a critical-ideological paradigm. Themes reflecting experiences related to race and role power dynamics were identified. RESULTS: Our cohort had 60 participants: 42 (70%) were support staff (medical assistants, front desk clerks, care navigators, nurses) and 18 (30%) were clinicians and clinic leaders. The large majority of participants were aged 26 to 40 years (60%), were female (83%), and were POC (68%). Five themes emerged: (1) POC face hidden challenges, (2) racial discrimination persists, (3) power dynamics perpetuate inaction, (4) interpersonal actions foster safety and equity, and (5) system-level change is needed for cultural shift. CONCLUSIONS: Understanding the race and role power dynamics within care teams, including experiences of staff members with less power, is critical to advancing DEI in health care.

Secret disclosure and social relationships in groups.

Personal secrets are a ubiquitous fact of group life, but the conditions under which they are revealed have not been explored. In five studies, we assessed secret disclosure in groups governed by four models of human sociality (Communal Sharing, Equality Matching, Authority Ranking, Market Pricing; Fiske). In Studies 1a and 1b, participants indicated their willingness to disclose secrets in hypothetical groups governed by the models. In Studies 2a and 2b, participants rated how much a group in which they disclosed secrets or nonsecrets is governed by the models. In Study 3, participants indicated their disclosure of various types of secrets in Communal Sharing and Equality Matching groups to which they belonged. Across studies, disclosure was most strongly associated with Communal Sharing, followed by Equality Matching. Study 3 further showed that identity fusion predicted disclosure in these two kinds of groups. Implications for understanding disclosure of personal secrets in group contexts were discussed.

Do sport teams with greater team resilience perceive higher performance at the end of the season? A multilevel analysis.

A team's ability to respond positively to adversities, problems, and obstacles during their season is an essential part of success in collective sports. Grounded in team resilience theory and using a multilevel analytical approach, this study examined the relationship of the characteristics of resilience and vulnerability under pressure with perceived individual and team performance. Participants were 676 soccer players (530 males and 146 females) aged 15-42 years (M = 21.40, SD = 5.38), who played on 64 senior and under-18 soccer teams of several national leagues in Spain. In the final month of the season, factors related to team resilience and individual and team performance were analyzed. We estimated multilevel models by including perceived individual and team performance as dependent variables. Characteristics of resilience and vulnerability under pressure were considered as fixed and random effects (i.e., individual- and team-level intercepts and slopes). At the individual level, results showed that characteristics of resilience were positively associated with subjective individual and team performance, whereas vulnerability under pressure was negatively related to perceived team (but not individual) performance. At the team level, only characteristics of resilience positively predicted team performance. These findings suggest that more resilient teams report more successful performance from an individual and team perspective, whereas teams that are more vulnerable under pressure report poorer team performance. Taken together, the study underscores the importance of practitioners to develop strategies that improve their teams' resilience, given that team resilience helps to achieve positive subjective individual and team outcomes.

Collective interactions, collaborative inhibition, and shared spatial knowledge.

Research on spatial mental representations focuses on individual mental maps and spatial knowledge. This exploratory study investigates instead collective interactions, collaborative memory, and the sharing of spatial knowledge. Based on the principle of collaborative inhibition (i.e., people recall information less effectively in groups), we posed the following research question: How do collective interactions, occurring during environmental exploration and group drawing sessions, affect collaborative inhibition, and the quality of sketch maps designed collectively? We conducted in situ explorations in Plaine St-Denis (France) with real-time tracking, followed by individual and group drawing sessions. This experiment involved 118 participants divided into three groups: (1) solo explorations without devices; (2) solo explorations with a mobile mapping application; (3) collective explorations using the same application enhanced with interaction features (viewing collective routes and photos of visited places). The comparison of the total number of entities found on individual mental maps with those included in collective sketch maps reveals that collaborative inhibition applies to spatial memory. Additional findings indicate that the use of a map, combined with collective interactions, mitigates collaborative inhibition and increases the accuracy of the sketch maps. However, the effect of such interactions on group dynamics remains unclear as of now.

A method for rewarding collaborative efforts in preclinical assessments.

Student success in basic medical science courses is typically determined by their individual performance on examinations of various types. Previous research both within and outside medical education has shown that the use of educational assessment activities can increase learning as demonstrated by performance on subsequent examinations, a phenomenon known as the testing effect. Activities primarily designed and used for assessment and evaluation purposes can also be used as teaching opportunities. We developed a method for measuring and evaluating student accomplishment in a preclinical basic science course that incorporates both individual and collaborative efforts, encourages and rewards active participation, does not compromise the reliability of the assessment outcome and is perceived by the students as helpful and valuable. The approach involved a two-part assessment activity composed of an individual examination and a small group examination with each component differentially weighted in determining an overall examination score. We found that the method was successful in encouraging collaborative efforts during the group component and provided valid measures of student grasp of the subject matter. We describe the development and implementation of the method, provide data derived from its use in a preclinical basic science course and discuss factors to be addressed when utilizing this approach to ensure fairness and reliability of the outcome. We include brief summary comments from students regarding their impressions of the value of this method.

The Psychometric Properties of Two Brief Measures of Teamwork in Sport.

In the current study, the structural and external validity of data derived from two shorter versions of the Multidimensional Assessment of Teamwork in Sport (MATS) were examined using multilevel analyses. Evidence of model-data fit was shown for both a 5-factor model comprising 19 items (with subscales assessing teamwork preparation, execution, evaluation, adjustments, and management of team maintenance) and a single-factor model comprising five items (providing a global estimate of teamwork). In general, data from both versions were positively and significantly correlated with (and distinct from) athletes' perceptions of team cohesion, collective efficacy, performance satisfaction, enjoyment in their sport, and commitment to their team and their coaches' transformational leadership. The measures appear well suited to detect between-teams differences, as evidenced by intraclass correlation coefficients and acceptable reliability estimates of team-level scores. In summary, the 19-item Multidimensional Assessment of Teamwork in Sport-Short and five-item Multidimensional Assessment of Teamwork in Sport-Global provide conceptually and psychometrically sound questionnaires to briefly measure teamwork in sport.

Paying attention to each other. An essay on the transnational intersections of industrial economy, subjectivity, and governance in East Germany's social-psychological training.

This article examines a little-known chapter both in the history of socialist labor relations and the history of psychology: Social Psychological Training (SPT) for industrial leaders in the German Democratic Republic (GDR). Based on previously untapped archival sources, it uncovers the transnational genesis of SPT and its intricate relationships with Western "therapeutic culture" of the 1970s. Governmental perspectives are addressed, as well as the level of individual appropriation of SPT and possible unintended side effects of techniques that were drawn from the social psychological and therapeutical fields. This case study helps to explore the functions of psychological expertise in authoritarian political contexts, as well as the polyvalence of group methods of change, the effects of which could turn out repressive as well as liberating on both sides of the Iron Curtain. The history of SPT solicits a polycentric view on therapeutic culture, capturing its diverse manifestations and interconnections between different societies and political economies.

The crying boss: Activating "human resources" through sensitivity training in 1970s Sweden.

This article examines the introduction of "sensitivity training" to 1970s Swedish work life. Drawing upon a range of empirical materials, I explore the politics that were involved in the process of translating and adapting this group dynamic method to the Swedish context and consider how its proponents argued for its value. By approaching sensitivity training as an attempt to govern, shape, and regulate both human beings and the work organizations of which they were a part, I argue that sensitivity training presents an unexpectedly early example of a governing rationality that has elsewhere been described and theorized as "neoliberal." The fact that sensitivity training was established in Swedish work life already in the early 1970s thus challenges the historiography of neoliberal modes of government, which have elsewhere been associated with a neoliberal shift in state policies occurring in the 1980s and 1990s. The article demonstrates how emotionally liberating practices in the late 1960s and early 1970s were embraced by some of the most politically influential actors in contemporary Swedish society, such as the corporate sector and the trade unions. As blue-collar trade unions and social democrats voiced increasingly far-reaching demands concerning workplace democracy and improved workplace conditions, advocates of sensitivity training presented their method as crucial to the process of "democratizing" and "humanizing" Swedish work life. Intimately associated with the new therapies of humanistic psychology, sensitivity training was used within the corporate sector to foster a more emotional and authentic leadership style that would embrace the values of emotional awareness, self-expression, and self-actualization. The crying boss emerged in this context as a key figure in the project of creating a "democratic" and psychologically satisfying organization. Yet, sensitivity training was also described as a means for companies to make better use of what was now asserted as their most important economic asset: the human being. From the outset, the idealistic vision of an emotionally liberated, democratic workplace was thus entangled with a specific kind of economic rationality, in which the emotionally liberated, self-actualizing individual emerged as a capital or asset that would be better utilized if the organization allowed-even encouraged-employees to engage in their own well-being and self-optimization.

[Case Conferences in Inpatient Child and Adolescent Psychotherapy - Single Case Presentation and General Recommendations]. / Die Fallbesprechung in der stationären Kinder- und Jugendpsychotherapie ­ Empfehlungen zum Vorgehen anhand einer Einzelfalldarstellung.

Case conference in a teamgroup in inpatient psychotherapy with children and adolescents are a tool to focus those aspects of treatment relations that can not (yet) be verbalized but present themselves in actions during the treatment. This paper gives a brief overview on the theory and methodology of case conferences and refers to an ongoing study. The presentation of a case conference leads to giving some general recommendations.

Social dynamics impact scolding behaviour in captive groups of common ravens (Corvus corax).

BACKGROUND: Predator avoidance can have immense impacts on fitness, yet individual variation in the expression of anti-predator behaviour remains largely unexplained. Existing research investigating learning of novel predators has focused either on individuals or groups, but not both. Testing in individual settings allows evaluations of learning or personality differences, while testing in group settings makes it impossible to distinguish any such individual differences from social dynamics. In this study, we investigate the effect of social dynamics on individual anti-predator behaviour. We trained 15 captive ravens to recognize and respond to a novel experimental predator and then exposed them to this predator in both group and isolation settings across 1.5 years to tease apart individual differences from social effects and evaluate two hypotheses: (1) weaker anti-predator responses of some individuals in the group occurred, because they failed to recognize the experimental predator as a threat, leading to weak responses when separated, or (2) some individuals had learned the new threat, but their scolding intensity was repressed in the group trials due to social dynamics (such as dominance rank), leading to increased scolding intensity when alone. RESULTS: We found that dominance significantly influences scolding behaviour in the group trials; top-ranked individuals scold more and earlier than lower ranking ones. However, in the separation trials scolding duration is no longer affected by rank. CONCLUSIONS: We speculate that, while top-ranked individuals use their anti-predator responses to signal status in the group, lower-ranking ravens may be suppressed from, or are less capable of, performing intense anti-predator behaviour while in the group. This suggests that, in addition to its recruitment or predator-deterrent effects, alarm calling may serve as a marker of individual quality to conspecifics.

Empathy and group processes in Japanese preschool children: The odd one out among friends receives less empathic concern than out-groups.

Research on empathy in intergroup contexts among children in collectivistic cultures is limited. To address this gap, this study examined empathic responding in two group contexts (intergroup and intragroup) among Japanese children by taking into account the collectivistic cultural context. Children aged 4 to 6 years participated in an experimental session (N = 50, Mage = 65.11 months). They listened to two versions of narratives about children of their age who were saddened because of a nasty wind that had blown their sand mountains away. The group membership and in-group status of the characters were manipulated. In the task, children rated the extent to which the characters were feeling sadness (affective perspective taking) and indicated the number of stars (empathic concern) for the characters. Age-related differences were found, with older children showing more affective perspective taking than younger children. Children of all age groups tended to express less empathic concern for the odd one out among friends (a loner in the group) than for the majority. Findings suggest that empathic responding is in part shaped by socialization, and cultural variations in empathy may emerge early in life.

Population characteristics associated with adherence to prenatal group-based exercise programs.

Community-based supervised group exercise may be an effective option to increase activity levels throughout pregnancy. Previous studies that have explored predictors of low adherence to exercise during pregnancy have not examined group-based settings. We analysed an international cohort of 347 pregnant women who participated in group-based prenatal exercise interventions (from <20 weeks to 34-36 weeks pregnant). Probable adherence predictors informed by previous literature that were assessed included: pre-pregnancy physical activity level and body mass index (BMI) classification, age, number of previous pregnancies, and education level. Adherence was measured by attendance. Adjusted odds ratios (aOR) and 95% confidence intervals (CI) were calculated to explore the relationship between the selected predictors and high adherence (≥70%). Post-secondary education level versus only secondary (aOR 5.28; CI 1.67; 16.72) or primary level (aOR 13.82; CI 4.30; 44.45) presented greater likelihood to have high adherence to the exercise intervention than low adherence. Future research and public health initiatives should consider implementing strategies to overcome education-related barriers to improve accessibility to prenatal exercise.

Feasibility and acceptability of a group-mediated exercise intervention for gynecological cancer survivors.

PURPOSE: Examine feasibility and acceptability of a group-mediated cognitive-behavioral (GMCB) intervention targeting planned, self-managed physical activity (PA). DESIGN: Sequential mixed methods, single arm pre-/post-test design with a 4-week follow-up. PARTICIPANTS: Post-treatment gynecologic cancer survivors. METHODS: Participants attended 8 weekly facilitator-led group sessions and completed assessments at baseline, post-intervention and follow-up. Feasibility was assessed by recruitment rate, retention rate, capture of outcomes, intervention usability and intervention fidelity. Acceptability was examined via qualitative interviews. Preliminary estimates of intervention effectiveness (PA, PA social cognitions and sleep) were collected. FINDINGS: 355 participants were approached and 38 consented. Twenty took part in the study and 17 (85%) completed the intervention. Thematic content analysis revealed positive group experiences. Cognitive-behavioral strategies were beneficial. Goal-setting and shared cancer recovery experience facilitated connection among group members. IMPLICATIONS: Program acceptability was high among a diverse sample of gynecologic cancer survivors and delivery of the program is feasible to this group of gynecologic cancer survivors. Recruitment challenges were present but study retention was high.

Modelling Spirals of Silence and Echo Chambers by Learning from the Feedback of Others.

What are the mechanisms by which groups with certain opinions gain public voice and force others holding a different view into silence? Furthermore, how does social media play into this? Drawing on neuroscientific insights into the processing of social feedback, we develop a theoretical model that allows us to address these questions. In repeated interactions, individuals learn whether their opinion meets public approval and refrain from expressing their standpoint if it is socially sanctioned. In a social network sorted around opinions, an agent forms a distorted impression of public opinion enforced by the communicative activity of the different camps. Even strong majorities can be forced into silence if a minority acts as a cohesive whole. On the other hand, the strong social organisation around opinions enabled by digital platforms favours collective regimes in which opposing voices are expressed and compete for primacy in public. This paper highlights the role that the basic mechanisms of social information processing play in massive computer-mediated interactions on opinions.

The Leader's Hypnotic Influence and the Creation of Alternate Reality.

Psychoanalysis has traditionally been an insular practice by analysts in their offices sequestered from any outside intrusion. However, in recent years a demand for psychoanalytic perspectives on the underlying dynamics of political figures and social phenomena has arisen. Media representatives have increasingly approached psychoanalysts for insight into such conditions as narcissistic personality disorder, compulsive lying, delusional thinking, when attempting to understand the irrational machinations of authoritarian leaders. Here, we will not be investigating the individual psyche, but rather the relationship between psyche and the culture of the populace (i.e., the polis). This paper considers the complex underlying dynamics of leaders' hypnotic influence and the creation of an alternate reality.

P-Curve Analysis of the Köhler Motivation Gain Effect in Exercise Settings: A Demonstration of a Novel Technique to Estimate Evidential Value Across Multiple Studies.

BACKGROUND: Practitioners and researchers may not always be able to adequately evaluate the evidential value of findings from a series of independent studies. This is partially due to the possibility of inflated effect size estimates for these findings as a result of researcher manipulation or selective reporting of analyses (i.e., p-hacking). In light of the possible overestimation of effect sizes in the literature, the p-curve analysis has been proposed as a worthwhile tool that may help identify bias across a series of studies focused on a single effect. The p-curve analysis provides a measure of the evidential value in the published literature and might highlight p-hacking practices. PURPOSE: Therefore, the purpose of this paper is to introduce the mechanics of the p-curve analysis to individuals researching phenomena in the psychosocial aspects of behavior and provide a substantive example of a p-curve analysis using findings from a series of studies examining a group dynamic motivation gain paradigm. METHODS: We performed a p-curve analysis on a sample of 13 studies that examined the Köhler motivation gain effect in exercise settings as a means to instruct readers how to conduct such an analysis on their own. RESULTS: The p-curve for studies examining the Köhler effect demonstrated evidential value and that this motivation effect is likely not a byproduct of p-hacking. The p-curve analysis is explained, as well as potential limitations of the analysis, interpretation of the results, and other uses where a p-curve analysis could be implemented.

'I felt like a human being'-An exploratory, multi-method study of refugee involvement in the development of mental health intervention research.

BACKGROUND: Great advancements have been made in patient and public involvement (PPI), including the development of guidance on how to conduct, report and evaluate PPI. Despite these efforts, the evidence base remains relatively weak. A substantive methodological development is required. This is particularly important for vulnerable groups within society, for whom PPI can be challenging but has the potential to play a transformative role in shaping research. OBJECTIVES: To describe the group dynamic characteristics and immediate impact of PPI from the user representatives' perspective in a case study of refugee involvement in the development of mental health intervention research. To pilot and methodologically appraise the Active Involvement of Users in Research Observation Schedule and Questionnaire. DESIGN: The Active Involvement of Users in Research Observation Schedule and Questionnaire were administered together with a focus group discussion. SETTING: 'Refugee Advisors' were involved in the development of a randomized controlled trial protocol evaluating a brief group intervention for refugee children experiencing symptoms of post-traumatic stress in Sweden. RESULTS: The multi-method approach demonstrated good feasibility. There were clear examples of how the advisors influenced research development. The advisors described a perceived impact on the research, equality and acceptance, and knowledge gain. A sense of appreciation and empowerment was also interpreted. However, potential issues relating to the relevance of contributions and use of an interpreter were identified. DISCUSSION AND CONCLUSION: The methodological approach piloted in this study offers a promising, rigorous way to evaluate PPI. The research tools require further refinement and validation.

More than just another bib: group dynamics in an elite Nordic ski team.

The social environment within individual sport teams can have a significant influence on the success, development, and well-being of athletes. We explored elite individual sport athletes' group experiences through the lens of social identity theorising. Participants were six members (4 male, 2 female, Mage = 21.5 years) of a National Development Nordic Ski team. At two time points in the competitive season, we used Social Identity Mapping (Bentley et al., 2020) in combination with semi-structured interviews to explore athletes' experiences as a member of the ski team. Social Identity Mapping provided a visual representation of each participant's social identities and was used to facilitate athletes' views of their group experiences in the semi-structured interviews. Interview data were thematically analysed to explicate participants' perceptions of social identity and cohesion, and their perceived relevance to success and development in elite individual sport. Major themes included social group memberships and identities, the presence of subgroups, the ebb and flow of cohesion and conflict, and teammate and coach influence on the group. On this basis, we argue that social identity mapping can be a valuable resource for athletes and coaches seeking to create a positive and cohesive team environment within an elite individual sport team.

The effect of group-dynamics, collaboration and tutor style on the perception of profession-based stereotypes: a quasi-experimental pre- post-design on interdisciplinary tutorial groups.

BACKGROUND: Group processes in inter-professional Problem-Based Learning (iPBL) groups have not yet been studied in the health-care educational context. In this paper we present findings on how group-dynamics, collaboration, and tutor style influence the perception of profession-based stereotypes of students collaborating in iPBL groups. Health-care students are trained in iPBL groups to increase their ability to collaborate with other healthcare professionals. Previous research focusing iPBL in healthcare implies that more systematic studies are desired, especially concerning the interaction between group processes and internalized professional stereotypes. The aim of this study is to investigate whether changes in group processes, collaboration, and tutor style, influence the perception of profession-based stereotypes of physician- and nursing-students. METHODS: The study is a quasi-experimental pre- post-design. The participants included 30 students from five different healthcare professions, mainly medicine and nursing. Other professions were physiotherapy, occupational therapy and speech therapy. The students were divided into four iPBL groups, each consisting of six to nine students and a tutor. Data were collected through systematic observation using four video-recorded tutorials. SPGR (Systematizing the Person Group Relation), a computer-supported method for direct and structured observation of behavior, was used to collect and analyze the data. RESULTS: Traditional stereotypical profession-based behaviors were identified in the first observed group meeting. Although the groups followed different paths of development, the group-dynamics changed in all groups over the 6 weeks of collaboration. Two of the groups became more cohesive, one became more fragmented and one became more polarized. Stereotypical behaviors became less frequent in all groups. Our findings indicate that tutor behavior has a strong influence on the development of the group's dynamics. CONCLUSION: Our findings strongly suggest iPBL is a means of reducing stereotypical behaviors, and may positively increase members' ability to engage in inter-professional collaboration. Although the pattern of dynamics took different forms in different groups, we argue that iPBL forces students to see the colleague behind his or her profession, thus breaking professional boundaries. The tutor style significantly influenced the iPBL groups' development. This study contributes to our field by emphasizing the effect of group-processes in increasing mutual understanding across professions.