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Cadmium (Cd)-enriched adsorbents wastes possess great environmental risk due to their large-scale accumulation and toxicity in the natural environment. Recycling spent Cd-enriched adsorbents into efficient catalysts for advanced applications could address the environmental issues and attain the carbon neutral goal. Herein, a facile strategy is developed for the first time to reutilize the alkali lignin (AL)-derived biochar (ALB) absorbed with Cd into cadmium sulphide (CdS)/C composite for the efficient methylene blue (MB) removal. The ALB is initially treated with Cd-containing solution, then the recycling ALB samples with adsorbed Cd are converted to the final CdS/C composite using NaS2 as the sulphurizing reagent for vulcanization reaction. The optimal ALB400 demonstrates a high adsorption capacity of 576.0 mg g-1 for Cd removal. Then the converted CdS/C composite shows an efficient MB removal efficiency of 94%. The photodegradation mechanism is mainly attributed to carbon components in the CdS/C composite as electron acceptor promoting the separation of photoelectrons/holes and slowing down the abrasion of CdS particles. The enhanced charge transfer and contact between the carrier and the active site thus improves the removal performance and reusability. This work not only develops a method for removing Cd from wastewater effectively and achieving the waste resource utilization but also further offers a significant guidance to use other kinds of spent heavy metal removal adsorbents for the construction of low-cost and high value-added functional materials.
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Iron deficiency is a major constraint for plant growth in calcareous soils. The interplay between NO3 - and Fe nutrition affects plant performance under Fe-deficient conditions. However, how NO3 - negatively regulates Fe nutrition at the molecular level in plants remains elusive. Here, we showed that the key nitrate transporter NRT1.1 in Arabidopsis plants, especially in the shoots, was markedly downregulated at post-translational levels by Fe deficiency. However, loss of NRT1.1 function alleviated Fe deficiency chlorosis, suggesting that downregulation of NRT1.1 by Fe deficiency favors plant tolerance to Fe deficiency. Further analysis showed that although disruption of NRT1.1 did not alter Fe levels in both the shoots and roots, it improved the reutilization of apoplastic Fe in shoots but not in roots. In addition, disruption of NRT1.1 prevented Fe deficiency-induced apoplastic alkalization in shoots by inhibiting apoplastic H+ depletion via NO3 - uptake. In vitro analysis showed that reduced pH facilitates release of cell wall-bound Fe. Thus, foliar spray with an acidic buffer promoted the reutilization of Fe in the leaf apoplast to enhance plant tolerance to Fe deficiency, while the opposite was true for the foliar spray with a neutral buffer. Thus, downregulation of the shoot-part function of NRT1.1 prevents apoplastic alkalization to ensure the reutilization of apoplastic Fe under Fe-deficient conditions. Our findings may provide a basis for elucidating the link between N and Fe nutrition in plants and insight to scrutinize the relevance of shoot-expressed NRT1.1 to the plant response to stress.
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Proteínas de Arabidopsis , Arabidopsis , Hierro/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Raíces de Plantas/metabolismo , Proteínas de Arabidopsis/metabolismo , Suelo , Regulación de la Expresión Génica de las Plantas , Nitratos/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Transporte de Anión/genéticaRESUMEN
Bromine is an important resource that is widely used in medical, automotive, and electronic industries. Waste electronic products containing brominated flame retardants can cause serious secondary pollution, which is why catalytic cracking, adsorption, fixation, separation, and purification have gained significant attention. However, the bromine resources have not been effectively reutilized. The application of advanced pyrolysis technology could help solve this problem via converting bromine pollution into bromine resources. Coupled debromination and bromide reutilization during pyrolysis is an important field of research in the future. This prospective paper presents new insights in terms of the reorganization of different elements and adjustment of bromine phase transition. Furthermore, we proposed some research directions for efficient and environmentally friendly debromination and reutilization of bromine: 1) precise synergistic pyrolysis should be further explored for efficient debromination, such as using persistent free radicals in biomass, polymer hydrogen supply, and metal catalysis, 2) rematching of Br elements and nonmetal elements (C/H/O) will be a promising direction for synthesizing functionalized adsorption materials, 3) oriented control of the bromide migration path should be further studied to obtain different forms of bromine resources, and 4) advanced pyrolysis equipment should be well developed.
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Residuos Electrónicos , Retardadores de Llama , Bromo , Bromuros , Pirólisis , Estudios Prospectivos , Residuos Electrónicos/análisisRESUMEN
In recent years, controlled low strength material (CLSM) has been utilized as an alternate backfill material for various infrastructure applications such as filling of voids, construction of pavement bases, trench backfilling, bed for pipelines, etc. Efforts have been made by researchers to utilize various waste materials/industrial by-products such as slag, fly ash, pond ash, cement kiln dust, red mud, sludge, construction and demolition waste and crumb rubber for development of sustainable CLSM. The present work discusses in details the evolution of CLSM, recent advances in the development of CLSM with different waste materials/industrial by-products, and the effect of these sustainable materials on flowability, strength, hardening time and other properties of CLSM. Further, the benefits/challenges and applications of different sustainable CLSM mixes have been compared. The inferences from pilot/field scale studies for CLSM and alkali activated CLSM have been discussed, and assessment of the sustainability coefficient of select CLSM combinations considered from the literature have been performed. The study quantifies the sustainability of different CLSM mixes, and presents the challenges that needs to be addressed in future to increase the utilization of sustainable CLSM for future infrastructure development.
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Ceniza del Carbón , Residuos , Aguas del Alcantarillado , Polvo , GomaRESUMEN
The reutilization non-metallic components from a waste-printed circuit board (WPCB) has become one of the most significant bottlenecks in the comprehensive reuse of electronic wastes due to its low value and complex compositions, and it has received great attention from scientific and industrial researchers. To effectively address the environmental pollution caused by inappropriate recycling methods, such as incineration and landfill, extensive efforts have been dedicated to achieving the high value-added reutilization of WPCB non-metals in sustainable polymer composites. In this review, recent progress in developing sustainable polymer composites based on WPCB non-metallic components was systematically summarized. It has been demonstrated that the WPCB non-metals can serve as a promising reinforcing and functional fillers to significantly ameliorate some of the physical and chemical properties of polymer composites, such as excellent mechanical properties, enhanced thermal stability, and flame retardancy. The recovery strategies and composition of WPCB non-metals were also briefly discussed. Finally, the future potentials and remaining challenges regarding the reutilization of WPCB non-metallic components are outlined. This work provides readers with a comprehensive understanding of the preparation, structure, and properties of the polymer composites based on WPCB non-metals, providing significant insights regarding the high value-added reutilization of WPCB non-metals of electronic wastes.
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Mining activities have often been associated with the issues of waste generation, while mining is considered a carbon-intensive industry that contributes to the increasing carbon dioxide emission to the atmosphere. This study attempts to evaluate the potential of reusing mining waste as feedstock material for carbon dioxide sequestration through mineral carbonation. Characterization of mining waste was performed for limestone, gold and iron mine waste, which includes physical, mineralogical, chemical and morphological analyses that determine its potential for carbon sequestration. The samples were characterized as having alkaline pH (7.1-8.3) and contain fine particles, which are important to facilitate precipitation of divalent cations. High amount of cations (CaO, MgO and Fe2O3) was found in limestone and iron mine waste, i.e., total of 79.55% and 71.31%, respectively, that are essential for carbonation process. Potential Ca/Mg/Fe silicates, oxides and carbonates have been identified, which was confirmed by the microstructure analysis. The limestone waste composed majorly of CaO (75.83%), which was mainly originated from calcite and akermanite minerals. The iron mine waste consisted of Fe2O3 (56.60%), mainly from magnetite and hematite, and CaO (10.74%) which was derived from anorthite, wollastonite and diopside. The gold mine waste was attributed to a lower cation content (total of 7.71%), associated mainly with mineral illite and chlorite-serpentine. The average capacity for carbon sequestration was between 7.73 and79.55%, which corresponds to 383.41 g, 94.85 g and 4.72 g CO2 that were potentially sequestered per kg of limestone, iron and gold mine waste, respectively. Therefore, it has been learned that the mine waste might be utilized as feedstock for mineral carbonation due to the availability of reactive silicate/oxide/carbonate minerals. Utilization of mine waste would be beneficial in light of waste restoration in most mining sites while tackling the issues of CO2 emission in mitigating the global climate change.
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Dióxido de Carbono , Secuestro de Carbono , Dióxido de Carbono/química , Minerales/química , Carbonato de Calcio/química , Carbonatos/análisis , Hierro , OroRESUMEN
INTRODUCTION: The reuse of cardiac implantable electronic devices may help increase access to these therapies in low- and middle-income countries (LMICs). No published data exist regarding the views of patients and family members in LMICs regarding this practice. METHODS AND RESULTS: An article questionnaire eliciting attitudes regarding pacemaker reuse was administered to ambulatory adult patients and patients' family members at outpatient clinics at Centro Nacional Cardiologia in Managua, Nicaragua, Indus Hospital in Karachi, Pakistan, Hospital Carlos Andrade Marín, and Hospital Eugenio Espejo in Quito, Ecuador, and American University of Beirut Medical Center in Beirut, Lebanon. There were 945 responses (Nicaragua - 100; Pakistan - 493; Ecuador - 252; and Lebanon - 100). A majority of respondents agreed or strongly agreed that they would be willing to accept a reused pacemaker if risks were similar to a new device (707, 75%), if there were a higher risk of device failure compared with a new device (584, 70%), or if there were a higher risk of infection compared to a new device (458, 56%). A large majority would be willing to donate their own pacemaker at the time of their death (884, 96%) or the device of a family member (805, 93%). Respondents who were unable to afford a new device were more likely to be willing to accept a reused device (79% vs. 63%, p < .001). CONCLUSIONS: Patients and their family members support the concept of pacemaker reuse for patients who cannot afford new devices.
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Desfibriladores Implantables , Marcapaso Artificial , Adulto , Equipo Reutilizado , Familia , Humanos , Encuestas y CuestionariosRESUMEN
Jasmonic acid (JA) is involved in phosphorus (P) stress in plants, but its underlying molecular mechanisms are still elusive. In this study, we found root endogenous JA content in rice increased under P deficiency (-P), suggesting that JA might participate in P homeostasis in plants. This hypothesis was further confirmed through the addition of exogenous JA (+JA), as this could increase both the root and shoot soluble P content through regulating root cell wall P reutilization. In addition, -P+JA treatment significantly induced the expression of P transporter gene OsPT2, together with increased xylem P content, implying that JA is also important for P translocation from the root to the shoot in P-deficient rice. Furthermore, the accumulation of the molecular signal nitric oxide (NO) was enhanced under -P+JA treatment when compared with -P treatment alone, while the addition of c-PTIO, a scavenger of NO, could reverse the P-deficient phenotype alleviated by JA. Taken together, our results reveal a JA-NO-cell wall P reutilization pathway under P deficiency in rice.
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Oryza , Pared Celular/metabolismo , Ciclopentanos/metabolismo , Óxido Nítrico/metabolismo , Oryza/genética , Oryza/metabolismo , Oxilipinas/metabolismo , Fósforo/metabolismo , Raíces de Plantas/metabolismoRESUMEN
High-silica phosphogypsum (PG) is a kind of industrial by-product with great utilization potential. However, it is difficult to reuse PG directly due to the related gangue minerals (e.g., SiO2), and thus efficient purification is required to allow its further applications. Herein, a typical high-silica phosphogypsum waste was purified by a new "reverse-direct flotation" method. The organic matters and fine slimes were removed by reverse flotation, and then, the silica impurity was removed by direct flotation. Via the closed-circuit flotation process, the whiteness of the PG concentrate is improved from 33.23 to 63.42, and the purity of gypsum in the PG concentrate increases from 83.90% to 96.70%, with a gypsum recovery of 85%. Additionally, the content of SiO2 is significantly reduced from 11.11% to 0.07%. In-depth investigations suggest that the difference in the floatability of gypsum and quartz is prominently intensified by flotation reagents at pH = 2-2.5, and thus leads to good desilication performance. Further characteristics of the PG concentrate prove that impurities have been well removed, and the PG concentrate meets the requirement of related standards for gypsum building materials. The flotation method reported here paves the way for the purification of high-silica phosphogypsum, which can be extended to the purification and value-added reutilization of other industrial solid wastes.
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Sulfato de Calcio , Dióxido de Silicio , Sulfato de Calcio/química , Residuos Industriales/análisis , Fósforo/químicaRESUMEN
The toxicity and persistence of heavy metals has become a serious problem for humans. These heavy metals accumulate mainly in wastewater from various industries' discharged effluents. The recent trends in research are now focused not only on the removal efficiency of toxic metal particles, but also on their effective reuse as catalysts. This review discusses the types of heavy metals obtained from wastewater and their recovery through commonly practiced physico-chemical pathways. In addition, it covers the advantages of the new system for capturing heavy metals from wastewater, as compared to older conventional technologies. The discussion also includes the various structural aspects of trapping systems and their hypothesized mechanistic approaches to immobilization and further rejuvenation of catalysts. Finally, it concludes with the challenges and future prospects of this research to help protect the ecosystem.
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Metales Pesados/toxicidad , Reciclaje/métodos , Purificación del Agua/métodos , Adsorción , Catálisis , Humanos , Residuos Industriales/análisis , Metales Pesados/análisis , Metales Pesados/aislamiento & purificación , Reciclaje/tendencias , Aguas Residuales/química , Agua/análisis , Agua/química , Contaminantes Químicos del Agua/análisis , Purificación del Agua/estadística & datos numéricosRESUMEN
Plastic waste pollution has been identified as a serious global issue, posing environmental risks in terms of massive waste generation, ocean pollution, and increases in greenhouse gas (GHG) emissions. Despite documented environmental impacts, it remains debatable whether the global plastic waste trade (GPWT) for reutilization and recycling, as part of the global circular economy (CE), has historically contributed to environmental benefits. To investigate if historical GPWT has contributed to environmental benefits in terms of reductions of GHG emissions, this study analyzed GPWT between China and trading countries through their trajectories, characteristics and driving forces of reductions of GHG emissions between 1992 and 2017. Results indicated an increasing trend of reductions of GHG emissions in GPWT between China and trading countries over 25 years. A net reduction of 8.27 million metric tons carbon dioxide equivalent (CO2e) was observed in 2012, nearly 84 times levels observed in 1992. Policy implications after China's recent ban of imports of GPWT in December 2017 and recent changes of GPWT to other Asian countries are discussed. Dramatic changes in sustainable approaches to GPWT for reutilization and recycling are required.
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Gases de Efecto Invernadero , Asia , Dióxido de Carbono/análisis , China , Efecto Invernadero , PlásticosRESUMEN
The production of natural selenium (Se)-rich food by using a high-Se crop cultivar is beneficial to human health and environmental safety; however, the underlying mechanism of different Se-accumulation ability between high- and low-Se rice cultivars remains unclear. A low-grain-Se cultivar and high-grain-Se cultivar of rice were used as test materials, and two levels of Se (0 and 0.5 mg kg-1) were arranged in a randomized design containing twelve replicates. The dynamic changes of shoot Se concentration and accumulation, xylem sap Se concentration, shoot and grain Se distribution, Se transporters genes (OsPT2, Sultr1;2, NRT1.1B) expression of the high- and low-Se rice cultivars were determined. The shoot Se concentration and accumulation of the high-Se rice showed a greater degree of reduction than those of the low-Se rice during grain filling stage, indicating that leaves of high-Se rice served as a Se source and supplied more Se for the growth centre grain. The expression levels of OsPT2, NRT1.1B and Sultr1;2 in the high-Se rice cultivar were significantly higher than those in the low-Se rice cultivar, which indicated that the high-Se rice cultivar possessed better transport carriers. The distribution of Se in grain of the high-Se rice cultivar was more uniform, whereas the low-Se cultivar tended to accumulate Se in embryo end. The stronger reutilization of Se from shoots to grains promoted by increased transporters genes expression and optimized grain storage space may explain how the high-Se rice cultivar is able to accumulate more Se in grain.
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Oryza/fisiología , Selenio/metabolismo , Cadmio/metabolismo , Grano Comestible/química , Humanos , Oryza/metabolismo , Contaminantes del Suelo/análisis , Xilema/metabolismoRESUMEN
Soil contamination with metallic nanoparticles is increasing due to their increased use in industrial and domestic settings. These nanoparticles are potentially toxic to soil microbes and may affect their associated functions and thereby the nutrient cycling in agro-ecosystems. This study examined the effects of iron oxides nanoparticles (IONPs) on carbon (C) and nitrogen (N) dynamics of poultry (PM) and farmyard manure (FYM) in the soil. The application of IONPs increased iron content in soil microbial biomass, which reflected its consumption by the microbes. As a result, colony-forming units of bacteria and fungi reduced considerably. Such observations lead to a decrease in CO2 emission from PM and FYM by 27 and 28%, respectively. The respective decrease fractions in the case of N mineralization were 24 and 35%. Consequently, soil mineral N content was reduced by 16% from PM and 12% from FYM as compared to their sole application without IONPs. Spinach dry matter yield and apparent N recovery were increased by the use of organic waste (FYM, PM). The use of IONPs significantly reduced the plant N recovery fraction by 26 and 24% (P < 0.05) from PM and FYM, respectively. All the results mentioned above lead us to conclude that IONPs are toxic to soil microbes and affect their function i.e., carbon and N mineralization of applied manure, and thereby the on-farm N cycling from the manure-soil-plant continuum.
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Compuestos Férricos/toxicidad , Nanopartículas/toxicidad , Ciclo del Nitrógeno , Animales , Biomasa , Carbono , Ecosistema , Fertilizantes , Estiércol/microbiología , Nitrógeno , Aves de Corral , Suelo , Microbiología del SueloRESUMEN
Generally, the mechanical strength and stiffness of old corrugated cardboard (OCC) waste paper are decreased after multiple recycling procedures. Surface sizing starch, which is extensively used in the surface sizing of paper making, accumulates after dissolving from the fibers and is transformed into pollutant during the OCC re-pulping process. To overcome the pollution and reutilization problem of the waste starch during the recycling process of OCC paper, waste starch was ionized using hydrogen peroxide (H2O2) to improve the mechanical properties of OCC paper during the reutilization. The results showed that the carboxyl group of waste starch increased with an increasing degree of ionization, resulting in enhanced copper ion adsorption capacity. Furthermore, the retention rate of the modified starch in the wet-end increased from 18.0% to 48.2%. The OCC paper presented the highest burst index and tensile strength of 8.94 kPa m2/g and 112.5 N m/g, respectively, when MS-2 was added. This work has great significance for implementation of the cleaning production of OCC waste papers and the reutilization of the waste starch.
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Peróxido de Hidrógeno , Almidón , Papel , Reciclaje , Resistencia a la TracciónRESUMEN
Nitrogen is one of the most important nutrient element that is essential for plant growth and development. Many genes have been reported to contribute to nitrogen absorption and transportation. However, genes involved in nitrogen reutilization are seldom reported. Ornithine δ-aminotransferase (δOAT) is the enzyme connecting arginine cycling and proline cycling. Here, we found that OsOAT, the homologue of δOAT in rice, is essential for nitrogen reutilization through mediating arginase activity. In the Osoat mutant, metabolic abnormality induced by nitrogen deficiency in floret causes malformed glumes, incapable glume opening and anther indehiscence. These defects in the mutant affect the pollination process and lead to a low seed setting rate as well as abnormal seed shape. Intriguingly, urea can rescue the phenotypes of the Osoat mutant. Therefore, OsOAT is crucial for nitrogen reutilization and plays a critical role in floret development and seed setting in rice.
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Nitrógeno/metabolismo , Ornitina-Oxo-Ácido Transaminasa/genética , Ornitina-Oxo-Ácido Transaminasa/metabolismo , Oryza/enzimología , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Semillas/metabolismo , Arginasa/metabolismo , Arginina/metabolismo , Regulación de la Expresión Génica de las Plantas , Redes y Vías Metabólicas/genética , Mutación , Oryza/genética , Oryza/crecimiento & desarrollo , Fenotipo , Proteínas de Plantas/genética , Plantas Modificadas Genéticamente/metabolismo , Polinización , Semillas/crecimiento & desarrollo , Alineación de Secuencia , Análisis de Secuencia de Proteína , Transcriptoma , Urea/metabolismoRESUMEN
Large amounts of agro-industrial waste are being generated each year, leading to pollution and economic loss. At the same time, these side streams are rich source of active compounds including antioxidants. Recovered compounds can be re-utilized as food additives, functional foods, nutra-/pharmaceuticals, cosmeceuticals, beauty products, and bio-packaging. Advanced extraction techniques are promising tools to recover target compounds such as antioxidants from agro-industrial side streams. Due to the disadvantages of classical extraction techniques (such as large amounts of solvents, increased time of extraction, large amounts of remaining waste after the extraction procedure, etc.), and advanced techniques emerged, in order to obtain more efficient and sustainable processes. In this review paper aspects regarding different modern extraction techniques related to recovery of antioxidant compounds from wastes generated in different industries and their applications are briefly discussed.
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Agricultura , Antioxidantes , Cosmecéuticos , Residuos Industriales , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Cosmecéuticos/química , Cosmecéuticos/aislamiento & purificaciónRESUMEN
The genetic engineering of plants to facilitate the reclamation of soils and waters contaminated with inorganic pollutants is a relatively new and evolving field, benefiting from the heterologous expression of genes that increase the capacity of plants to mobilize, stabilize and/or accumulate metals. The efficiency of phytoremediation relies on the mechanisms underlying metal accumulation and tolerance, such as metal uptake, translocation and detoxification. The transfer of genes involved in any of these processes into fast-growing, high-biomass crops may improve their reclamation potential. The successful phytoextraction of metals/metalloids and their accumulation in aerial organs have been achieved by expressing metal ligands or transporters, enzymes involved in sulfur metabolism, enzymes that alter the chemical form or redox state of metals/metalloids and even the components of primary metabolism. This review article considers the potential of genetic engineering as a strategy to improve the phytoremediation capacity of plants in the context of heavy metals and metalloids, using recent case studies to demonstrate the practical application of this approach in the field.
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Ingeniería Genética , Metaloides/metabolismo , Metales Pesados/metabolismo , Plantas/genética , Contaminantes del Suelo/metabolismo , Biodegradación Ambiental , Plantas/metabolismo , Plantas Modificadas GenéticamenteRESUMEN
BACKGROUND: Pentacyclic triterpenic acids (TA) are phytochemicals of increasing nutritional interest owing to their bioactive properties, such as anti-inflammatory, antitumoral, antihyperglycemic and hepatoprotective. Crude olive pomace oils constitute a non-exploited significant source of these compounds. In the present study, concentrates of TA were extracted and characterized from crude olive pomace oils that were obtained by centrifugation and subsequent solvent extraction, respectively. Specifically, the concentrates were obtained from the byproduct generated in the filtration of the oils. The solids were subjected to Soxhlet extractions with hexane to remove the residual oil and then with ethanol for the TA extraction. RESULTS: Concentrates containing 850-980 g kg-1 TA were isolated from the oils obtained by centrifugation, whereas those isolated from oils obtained by hexane extraction presented levels of TA that ranged from 510 to 900 g kg-1 . Oleanolic (OA) and maslinic (MA) acids were the TA found in the concentrates. The relative contents of OA and MA were, respectively, 30:70 (w/w) and 77:23 (w/w). All concentrates also presented phenolic compounds at levels of g kg-1 and displayed slight antioxidant properties. CONCLUSION: Concentrates of TA, containing MA and OA, can be readily obtained from a byproduct generated by filtration of crude olive pomace oils. Concentrates isolated from oils obtained by centrifugation were rich in MA, whereas those from oils extracted with hexane were rich in OA. The concentrates showed slight antioxidant properties that can be mainly attributed to the presence of phenolic compounds and not to TA. © 2018 Society of Chemical Industry.
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Antioxidantes/química , Olea/química , Ácido Oleanólico/química , Aceite de Oliva/química , Extractos Vegetales/química , Triterpenos/química , Antioxidantes/aislamiento & purificación , Frutas/química , Ácido Oleanólico/aislamiento & purificación , Aceite de Oliva/aislamiento & purificación , Fenoles/química , Fenoles/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Triterpenos/aislamiento & purificaciónRESUMEN
Succinate is an important commodity chemical currently used in the food, pharmaceutical, and polymer industries. It can also be chemically converted into other major industrial chemicals such as 1,4-butanediol, butadiene, and tetrahydrofuran. Here we metabolically engineered a model cyanobacterium Synechococcus elongatus PCC 7942 to photosynthetically produce succinate. We expressed the genes encoding for α-ketoglutarate decarboxylase and succinate semialdehyde dehydrogenase in S. elongatus PCC 7942, resulting in a strain capable of producing 120mg/L of succinate. However, this recombinant strain exhibited severe growth retardation upon induction of the genes encoding for the succinate producing pathway, potentially due to the depletion of α-ketoglutarate. To replenish α-ketoglutarate, we expressed the genes encoding for phosphoenolpyruvate carboxylase and citrate synthase from Corynebacterium glutamicum into the succinate producing strain. The resulting strain successfully restored the growth phenotype and produced succinate with a titer of 430mg/L in 8 days. These results demonstrated the possibility of photoautotrophic succinate production.
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Ingeniería Metabólica , Fotosíntesis/genética , Ácido Succínico/metabolismo , Synechococcus , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Corynebacterium glutamicum/enzimología , Synechococcus/genética , Synechococcus/metabolismoRESUMEN
Bone is one of the most transplanted tissues. While most bone defects heal spontaneously, critical size defects caused by major trauma/malignant tumor and osteonecrosis of femoral head in young adults pose a great challenge in treatment. While the golden standard in treating bone defects is autologous bone grafting, available bone for grafting is quite limited in an individual. To solve the dilemma, stem cell therapy has been tried as a new modality of treatment in lesions not amenable to autologous bone grafting. While successful results were reported from individual studies, the stem cell therapy is still not an established treatment modality for bone regeneration and needs further assessment. Our focus herein is to introduce stem cell sources that have been investigated so far and review the current status of stem cell reutilization for bone regeneration as well as suggesting future perspectives.