Molecular Characterization of Chemosensory Protein (CSP) Genes and the Involvement of AgifCSP5 in the Perception of Host Location in the Aphid Parasitoid Aphidius gifuensis.

Aphidius gifuensis is the dominant parasitic natural enemy of aphids. Elucidating the molecular mechanism of host recognition of A. gifuensis would improve its biological control effect. Chemosensory proteins (CSPs) play a crucial role in insect olfactory systems and are mainly involved in host localization. In this study, a total of nine CSPs of A. gifuensis with complete open reading frames were identified based on antennal transcriptome data. Phylogenetic analysis revealed that AgifCSPs were mainly clustered into three subgroups (AgifCSP1/2/7/8, AgifCSP3/9, and AgifCSP4/5/6). AgifCSP2/5 showed high expression in the antennae of both sexes. Moreover, AgifCSP5 was found to be specifically expressed in the antennae. In addition, fluorescent binding assays revealed that AifCSP5 had greater affinities for 7 of 32 volatile odor molecules from various sources. Molecular docking and site-directed mutagenesis results revealed that the residue at which AgifCSP5 binds to these seven plant volatiles is Tyr75. Behavior tests further confirmed that trans-2-nonenal, one of the seven active volatiles in the ligand binding test, significantly attracted female adults at a relatively low concentration of 10 mg/mL. In conclusion, AgifCSP5 may be involved in locating aphid-infested crops from long distances by detecting and binding trans-2-nonenal. These findings provide a theoretical foundation for further understanding the olfactory recognition mechanisms and indirect aphid localization behavior of A. gifuensis from long distances by first identifying the host plant of aphids.

Effects of relative humidity on physiology and behavior of laying hens exposed to high ambient temperatures.

This study investigated how different relative humidity (RH) would impact behavior and physiology of laying hens. One hundred-eighty laying hens (Hy-Line Brown), aged 68-week-old (1,908 ± 78 g), were exposed to daily ambient temperatures of 30 °C with RH set at 25% (RH25), 50% (RH50), or 75% (RH75) for 12 h a day (9:00 am to 9:00 pm) from 70 to 74 weeks of age. All hens had been raised at 24 °C and 50% RH during 2-week adaptation period. Each RH treatment consisted of 10 replicates with 6 hens each in a completely randomized design. Results indicated that rectal temperature, respiratory rate, and heart rate were increased (P ≤ 0.05) in the RH75 group compared with the RH25 and RH50 groups. Plasma malondialdehyde concentrations were elevated in the RH75 group compared RH50 at 3, 7, and 21 days (P ≤ 0.05) following the RH exposure. Plasma glutathione peroxidase activity was lowered (P ≤ 0.05) in the RH75 group compared with the RH25 and RH50 groups at 21 days. Increasing RH led to decreased feeding behavior but showed a tendency to increase drinking behavior. Wings elevated and panting behaviors were higher in the RH75 group compared with the RH25 and RH50 groups (P ≤ 0.05). In conclusion, these findings suggest that elevated RH at constant ambient temperature could negatively influence the behavior and physiology of laying hens.

Prey species increase activity in refugia free of terrestrial predators.

The decline of terrestrial predator populations across the globe is altering top-down pressures that drive predator-prey interactions. However, a knowledge gap remains in understanding how removing terrestrial predators affects prey behavior. Using a bifactorial playback experiment, we exposed fox squirrels to predator (red-tailed hawks, coyotes, dogs) and non-predator control (Carolina wren) calls inside terrestrial predator exclosures, accessible to avian predators, and in control areas subject to ambient predation risk. Fox squirrels increased their use of terrestrial predator exclosures, a pattern that corresponded with 3 years of camera trapping. Our findings suggest fox squirrels recognized that exclosures had predictably lower predation risk. However, exclosures had no effect on their immediate behavioral response towards any call, and fox squirrels responded most severely to hawk predator calls. This study shows that anthropogenically driven predator loss creates predictably safer areas (refugia) that prey respond to proactively with increased use. However, the persistence of a lethal avian predator is sufficient to retain a reactive antipredator response towards an immediate predation threat. Some prey may benefit from shifting predator-prey interactions by gaining refugia without sacrificing a sufficient response towards potential predators.

Aerosol Alteration of Behavioral Response to Pheromone in Bombyx mori.

Because of the complexity to study them, aerosols have been neglected in nearly all studies on olfaction, especially studies dealing with odor capture. However, aerosols are present in large quantities in the atmosphere and have the physico-chemical ability to interact with odor molecules, in particular the many pheromones with low volatility. We submitted male moths of Bombyx mori to bombykol puffs, the main fatty alcohol component of its sex pheromone, depending on whether the air is free of aerosols, charged with ambient concentration aerosols or supplemented with aqueous aerosols and recorded their arousal behavior. Aerosols and pheromone do interact consistently over all experiments and moths react better in low aerosol-concentration conditions. We propose four hypotheses for explaining this impediment, the two most likely resorting to competition between odor molecules and aerosols for the olfactory pores and postulate a reversal to a positive impact of aerosols on communication, depending on the particular physico-chemical properties of the multiphasic interaction. Studying the partitioning between gas and particulate phases in the transport and reception of odors is key for advancing the chemico-physical understanding of olfaction.

Effect of perinatal dietary protein deficiency on some neurochemicals and cytoarchitectural balance, in F1 and F2 generations of rats.

ABSTRACTProtein deficiency, characterized by an inadequate intake of protein in the diet that fails to meet the body's physiological requirements across various stages, can lead to detrimental outcomes. This is of interest due to the persistent low protein content in staple foods and suboptimal dietary patterns. The study sought to assess the intergenerational repercussions of dietary protein deficiency on specific neurochemicals and the cytoarchitecture of the brain within the F1 and F2 generations of rats. The rats were categorized into four groups based on the protein content percentage in their diets: 21% protein diet (21%PD), 10% protein diet (10%PD), 5% protein diet (5%PD), and control diet. Neurobehavior was assessed, while brain serotonin and dopamine levels were measured using HPLC. BDNF and GDNF expression in the hippocampal and prefrontal (PFC) sections, Immunohistochemical investigations of the morphological impact on the hippocampus and PFC, were also analyzed. The protein-deficient groups displayed anxiety, loss of striatal serotonin and increased dopamine levels, degenerated pyramidal cells in the hippocampus, and a prominent reduction in cellular density in the PFC. BDNF and GDNF levels in the PFC were reduced in the 5%PD group. GFAP astrocyte expression was observed to be increased in the prefrontal cortex (PFC) and hippocampal sections, indicating heightened reactivity. The density of hypertrophied cells across generations further suggests the presence of neuroinflammation. Changes in brain structure, neurotransmitter levels, and neurotrophic factor levels may indicate intergenerational alterations in critical regions, potentially serving as indicators of the brain's adaptive response to address protein deficiency across successive generations.

The Orco gene involved in recognition of host plant volatiles and sex pheromone in the chive maggot Bradysia odoriphaga.

The insect olfactory recognition system plays a crucial role in the feeding and reproductive behaviors of insects. The odorant receptor co-receptor (Orco), as an obligatory chaperone, is critical for odorant recognition by way of forming heteromeric complexes with conventional odorant receptors (ORs). To investigate the biological functions of Orco in perceiving host plant volatiles and sex pheromone, the Orco gene was identified from the chive maggot Bradysia odoriphaga transcriptome data. Multiple sequence alignment reveals that BodoOrco exhibits an extremely high sequence identity with Orcos from other dipteran insects. The expression of BodoOrco is significantly higher in adults than in larvae and pupae, and the BodoOrco gene is primarily expressed in the antennae of both sexes. Furthermore, the Y-tube assay indicated that knockdown of BodoOrco leads to significant reductions in B. odoriphaga adults' response to all tested host plant volatiles. The dsOrco-treated unmated male adults show less attraction to unmated females and responded slowly compared with dsGFP control group. These results indicated that BodoOrco is involved in recognition of sex pheromone and host plant volatiles in B. odoriphaga and has the potential to be used as a target for the design of novel active compounds for developing ecofriendly pest control strategies.

Expression and functional analysis of Niemann-Pick C2 gene in Phytoseiulus persimilis.

As a new protein class of semiochemical binding and transporting, Niemann-Pick proteins type C2 (NPC2) in arthropods have received more attentions in recent decade. However, the gene function has not been studied in phytoseiid mites with biocontrol potential. In the current study, we cloned a NPC2 gene PpNPC2a from the transcriptome of Phytoseiulus persimilis Athias-Henriot. By encoding 181 amino acids with a conserved ML domain, PpNPC2a was found a homolog of NPC2-1 in Galendromus occidentalis Nesbitt. We then measured the spatio-temporal expression of PpNPC2a in P. persimilis, and found the highest expression in female adults compared to other stages. Due to the tiny body size of predatory mites, we only examined tissue expressions in two sections: the anterior part (gnathosoma and the first pair of legs) and the posterior part (idiosoma without the first pair of legs). Higher transcription of PpNPC2a was found in the posterior part. To investigate the potential function of PpNPC2a in P. persimilis, we interfered gene expression in female adults by feeding dsRNA, which resulted in a decrease of relative expression by 59.1 and 78.2% after 24 and 72 h, respectively. Compared with the control, dsNPC2a-treated P. persimilis were insensitive to the scent of leaves or plants infested by spider mites, suggesting a role of PpNPC2a in response to plant volatiles. However, the dsNPC2a-interfered mites could still respond to four representative compounds of herbivore-induced plant volatiles, including 4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), methyl salicylate (MeSA), ß-caryophyllene and linalool. In short, our results indicated PpNPC2a may be involved in the chemosensory process of P. persimilis in response to whole-plant volatiles.

Features of phototropic response of zooplankton to paired photostimulation under adverse environmental conditions.

Our previous studies showed that the change in the plankton response to light could be an indicator of environmental pollution. This study experimentally reveals that the response of Daphnia magna Straus and Daphnia pulex plankton ensembles to photostimulation depends on the intensity of the attracting light. This makes it difficult to identify the occurrence and change of pollutant concentration. The large variability in the magnitude of the behavioral response is caused by the nonlinear response of plankton ensembles to the intensity of the attractor stimulus. As the intensity of the photostimulation increases, the variability of the phototropic response passes through increase, decrease, and relative stabilization phases. The paper proposes a modification of the photostimulation method-paired photostimulation involving successive exposure to two photostimuli of increasing intensity. The first stimulus stabilizes the behavioral response, while the increase in response to the second stimulus makes it possible to more accurately assess the responsiveness of the plankton ensemble. The paper studies the sensitivity of the method of paired stimulation of the behavioral response of different types of freshwater plankton ensembles: Daphnia magna Straus, Daphnia pulex to the effects of pollutants (potassium bichromate, microplastic). The study demonstrates good reliability and increased sensitivity of this method of detecting changes in environmental toxicity compared to single photostimulation or traditional bioindication through the survival rate of test organisms.

Habituation in anuran tadpoles and the role of risk uncertainty.

The ability to learn in the context of predation allows prey to respond to threats by adjusting their behavior based on specific information acquired from their current environment. Habituation is a process that allows animals to adapt to environmental changes. Very little is known about habituation in wild animals in general and there are no studies on habituation in anuran tadpoles in particular. Here, we performed three experiments to investigate the behavioral response of predator naïve Pleurodema thaul tadpoles to repeated stimulation with two predation risk cues (injured conspecific and predator fed cues) which a priori provide different information regarding risk. Experiment 1 showed that P. thaul tadpoles habituate the antipredator response when undergo predation risk chemical cues from injured conspecific and that response is long term. Experiment 2 showed that P. thaul tadpoles did not habituate their antipredator response when exposed to cues derived from an event of nymph odonate preying on P. thaul tadpoles (predator fed cues). Experiment 3 specifically evaluated the risk imposed by each of the risk cues used in Experiment 1 and Experiment 2 and showed that the degree of perceived risk in tadpoles appear to be similar in a single experience with any risk stimuli. We suggest that the behavioral habituation of tadpoles in the context of predation could be modulated by the level of uncertainty associated with risk stimuli.

Sublethal Exposure to Cadmium Induces Chemosensory Dysfunction in Fire Ants.

Ants easily accumulate cadmium (Cd) from the food web in terrestrial ecosystems. Cd contamination may cause olfactory dysfunction and consequently disorders in the social behavior of ants. To explore the molecular mechanism underlying the effect of Cd exposure on the chemosensory process of ants, we characterized the Cd-induced variations in the expression of genes involved in chemoreception and electrophysiological and behavioral sensitivity to semiochemicals by using the red imported fire ant, Solenopsis invicta, as a model system. As a result, Cd exposure increased Cd accumulation and decreased the survival rate of S. invicta. Cd exposure altered the expression profiles of odor binding protein genes of S. invicta (SiOBPs). Specifically, SiOBP15 protein expression was upregulated upon Cd exposure. Both SiOBP7 and SiOBP15 exhibited high binding affinities to limonene, nonanal, and 2,4,6-trimethylpyridine. S. invicta exposed to Cd showed less sensitive electrophysiological and behavioral response to the three chemicals but exhibited sensitive perception to undecane. Silencing of SiOBP7 and SiOBP15 abolished the behavioral response of S. invicta to nonanal and undecane, respectively, suggesting that SiOBP7 and SiOBP15 play essential roles in the chemoreception of S. invicta. In general, our results suggest that Cd contamination may interfere with olfactory signal transduction by altering the expression of SiOBPs, consequently evoking chemosensory dysfunction in fire ants.

Prey tells, large herbivores fear the human 'super predator'.

Fear of the human 'super predator' has been demonstrated to so alter the feeding behavior of large carnivores as to cause trophic cascades. It has yet to be experimentally tested if fear of humans has comparably large effects on the feeding behavior of large herbivores. We conducted a predator playback experiment exposing white-tailed deer to the vocalizations of humans, extant or locally extirpated non-human predators (coyotes, cougars, dogs, wolves), or non-predator controls (birds), at supplemental food patches to measure the relative impacts on deer feeding behavior. Deer were more than twice as likely to flee upon hearing humans than other predators, and hearing humans was matched only by hearing wolves in reducing overall feeding time gaged by visits to the food patch in the following hour. Combined with previous, site-specific research linking deer fecundity to predator abundance, this study reveals that fear of humans has the potential to induce a larger effect on ungulate reproduction than has ever been reported. By demonstrating that deer most fear the human 'super predator', our results point to the fear humans induce in large ungulates having population- and community-level impacts comparable to those caused by the fear humans induce in large carnivores.

Odor Perception in the Cotton Bollworm, Helicoverpa armigera, Exposed to Juglans regia, a Marginal Host Plant.

The cotton bollworm, Helicoverpa armigera, is one of the most destructive agricultural pests in the world, infesting cotton, maize, soybean, and many other crops. In recent years, H. armigera has been observed damaging walnuts, Juglans regia, in Xinjiang China. Here we examine the chemical perception by H. armigera of the marginal host J. regia. In Y-tube olfactometer tests, we found H. armigera females and males both showed significant behavioral responses to odors from walnut branches. Furthermore, nine electrophysiologically active volatiles (α-pinene, ß-pinene, myrcene, limonene, eucalyptol, ocimene, ß-caryophyllene, (E)-ß-farnesene, and germacrene D) were identified from walnuts with gas chromatography coupled with electroantennography (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). Among these volatiles, ß-pinene and eucalyptol were released in relatively higher amounts. In electroantennogram (EAG) dose-dependent trials, all compounds evoked responses in H. armigera adults when tested at high concentrations, with germacrene D evoking the greatest response. In wind tunnel tests, H. armigera females preferred eight of the electrophysiologically active volatile dilutions compared with clean air, while males showed preference for only five compounds. As such we describe the chemical recognition of H. armigera for walnut, a marginal host. This study contributes to understanding the interaction between polyphagous pests and their host plants.

Behavioral responses for facemask use messages to prevent COVID-19 among residents of Bahir Dar City, Ethiopia: an application of extended parallel process model.

BACKGROUND: The coronavirus disease 2019 (COVID-19) pandemic causes major morbidity and mortality in the world. Timely behavioral response assessment of the community is important to shape the next effective interventions and risk communication strategies to adopt preventive behavior. Hence, this study aimed to assess behavioral responses for facemask-use messages to prevent COVID-19 and its predictors among residents of Bahir Dar City, Ethiopia, 2021 by using the Extended Parallel Process Model.  METHODS: A community-based cross-sectional study was conducted with the guide of the Extended Parallel Process Model in Bahir Dar city from March 9 to April 9, 2021. A multistage sampling technique was used, and data was collected through a face-to-face interviewer-administered questionnaire using Epicollect5. Descriptive statistics and Binary logistic regression were computed using SPSS V.25. Variable with P < 0.25 in the bivariable analysis was a candidate for multivariable analysis to control confounding effect. In multivariable analysis, variables with P < 0.05 were considered statistically significant and the result was presented using an adjusted odd ratio (AOR) with a 95% confidence interval (CI). RESULTS: A total of 616 participants with a response rate of 97.1% were included. Of the total participants, 229(37.2%) were in the danger control response. The behavioral response was affected by Occupational status [AOR (95%CI) 3.53(1.67-7.46)], the number of people living together [AOR (95%CI) 2.62(1.28-5.39)], self-control [AOR (95%CI) 1.14(1.05-1.25)], a friend for the preferred source of information [AOR (95%CI) 5.18(3.22-8.33)] and printed materials for the preferred channel [AOR (95%CI) 2.14(1.35-3.43)]. CONCLUSION: Above one-third of the participants were in the danger control response. Occupational status, number of people living together, self-control, a friend for the preferred source of information, and printed materials for the preferred channel were independent predictors of resident behavioral response to the use of facemasks. Policymakers should consider students and people who live alone. Message developers should use a friendly person to transmit messages and should prepare printed materials. Activities and strategies should also focus on self-control and perceived efficacy without ignoring the perceived threat.

Life during COVID-19 lockdown in Italy: the influence of cognitive state on psychosocial, behavioral and lifestyle profiles of older adults.

OBJECTIVE: Few studies have examined lockdown effects on the way of living and well-being of older adults stratified by cognitive state. Since cognitive deficits are common in this population, we investigated how cognition influenced their understanding of the pandemic, socio-behavioral responses and lifestyle adaptations during lockdown, and how these factors affected their mood or memory. METHOD: Telephone-based survey involving 204 older adults ≥65 y/o (median: 82) with previous assessments of cognitive state: 164 normal-old (NOLD), 24 mild-neurocognitive disorder (mild-NCD), 18 mild-moderate dementia. A structured questionnaire was developed to assess psychological and socio-behavioral variables. Logistic regression was used to ascertain their effects on mood and memory. RESULTS: With increasing cognitive deficits, understanding of the pandemic and the ability to follow lockdown policies, adapt to lifestyle changes, and maintain remote interactions decreased. Participants with dementia were more depressed; NOLDs remained physically and mentally active but were more bored and anxious. Sleeping and health problems independently increased the likelihood of depression (OR: 2.29; CI: 1.06-4.93; p = 0.034 and OR: 2.45; CI: 1.16-5.16; p = 0.018, respectively); Regular exercise was protective (OR: 0.30; CI: 0.12-0.72; p = 0.007). Worsening subjective memory complaints were associated with dementia (p = 0.006) and depression (p = 0.004); New-onset sleeping problems raised their odds (OR: 10.26; CI: 1.13-93.41; p = 0.039). Finally, >40% with health problems avoided healthcare mainly due to fear of contagion. DISCUSSION: NOLD and mild-NCD groups showed similar mood-behavioral profiles suggesting better tolerance of lockdown. Those with dementia were unable to adapt and suffered from depression and cognitive complaints. To counteract lockdown effects, physical and mental activities and digital literacy should be encouraged.

State and trait anxiety among medical staff during the first month of COVID-19 pandemic: A sample from Turkey.

OBJECTIVES: During the COVID-19 pandemic, excessive workload, a rapidly changing workplace environment, the danger of carrying the virus and transmitting the disease to their families, relatives and those they live with creates stress for the medical workers. In our study, we aimed to evaluate the state and trait anxiety levels of healthcare professionals who encounter patients with suspected COVID-19 infection and related factors. METHOD: Data were collected from healthcare professionals working with patients diagnosed or suspected with COVID-19 via online self-report questionnaire between 9-19 April 2020. The state (STAI-S) and trait anxiety (STAI-T) scale was used to measure anxiety. RESULTS: A total of 291 healthcare professionals, 216 women and 75 men, participated in the study. Women's state and trait anxiety were significantly higher than men's. 11 participants without any lifetime psychiatric illness experienced psychiatric symptoms and consulted to a psychiatrist. The state anxiety of those who have children, nurses and those working in branches directly related to the pandemic (Infectious Diseases, Respiratory Diseases, Emergency Medicine, Internal Medicine, Radiology, Anesthesiology and Reanimation) was higher than others. The state anxiety of those who thought they were not protected with personal protective equipment and those who did not stay in their own home was higher than others. CONCLUSIONS: At the forefront of the fight against COVID-19, there are medical personnel who pay a serious psychological cost. Especially in terms of anxiety, we should pay attention to women, workers with children, nurses and people working in branches that are directly related to pandemics.

Acute Toxicity Evaluation of the Disinfectant Containing Percarbonate and Tetraacetylethylenediamine by Measuring Behavioral Responses of Small Fish Using Image Analysis.

Disinfectants containing percarbonate and tetraacetylethylenediamine (TAED) has been developed as an effective and relatively safe disinfectant to destroy viruses and bacteria in animals and humans, however it is known that most disinfectants can cause danger to living organisms including humans. In the current study, acute toxicity of the disinfectant composed of percarbonate and TAED was assessed by measuring behavioral responses as well as lethal concentrations of aquatic organisms such as medaka and zebrafish when they were exposed to it. First, the breeding water properties were determined by measuring dissolved oxygen (DO) and pH changes over time up to 96 h in acute toxicity tests using the medaka, and the lethal concentration 50% (LC50, 88.39 ppm) was calculated using the lethality rate of the fish. This experiment was conducted in compliance with traditional OECD guidelines. Second, the assessment of behavioral responses (locomotive activity and swimming speed) with the zebrafish were assessed by the image analysis to capture the images per second for three hours, and the collected data were processed using image analysis to calculate the locomotive activity and swimming speed. Finally, the LC50 (135.76 ppm) of the disinfectant to the fish was also measured after three hours. Overall, the data revealed that LC50 of the disinfectant may be affected by the pH of the water exposed to the disinfectant, not by the DO in the water. In addition, the results from the image analysis indicated that the behavioral responses of the fish can further assess the acute toxicity of the disinfectant at concentrations below the LC50 and there was a relationship (R2 = 0.85) between the behavioral responses and the survival rate of the fish. Electronic Supplementary Material ESM: The online version of this article (doi: 10.1007/s12257-021-0419-0) contains supplementary material, which is available to authorized users.

Western gray whale behavioral response to seismic surveys during their foraging season.

Gray whales utilizing their foraging grounds off northeastern Sakhalin Island, Russia, have been increasingly exposed to anthropogenic activities related to oil and gas development over the past two decades. In 2015, four seismic vessels, contracted by two operators, conducted surveys near and within the gray whale feeding grounds. Mitigation and monitoring plans were developed prior to the survey and implemented in the field, with real-time data transfers to assist the implementation of measures aimed at minimizing impacts of acoustic exposure. This study examined the behavioral response of gray whales relative to vessel proximities and sounds generated during seismic exploration. Five shore-based teams monitored gray whale behavior from 1 June to 30 September using theodolite tracking and focal follow methodologies. Behavioral data were combined with acoustic and benthic information from studies conducted during the same period. A total of 1270 tracks (mean duration = 0.9 h) and 401 focal follows (1.1 h) were collected with gray whales exposed to sounds ranging from 59 to 172 dB re 1 µPa2 SPL. Mixed models were used to examine 13 movement and 10 respiration response variables relative to "natural," acoustic, and non-acoustic explanatory variables. Water depth and behavioral state were the largest predictors of gray whale movement and respiration patterns. As vessels approached whales with increasing seismic/vessel sound exposure levels and decreasing distances, several gray whale movement and respiration response variables significantly changed (increasing speed, directionality, surface time, respiration intervals, etc.). Although the mitigation measures employed could have reduced larger/long-term responses and sensitization to the seismic activities, this study illustrates that mitigation measures did not eliminate behavioral responses, at least in the short-term, of feeding gray whales to the activities.

Real-time acoustic monitoring with telemetry to mitigate potential effects of seismic survey sounds on marine mammals: a case study offshore Sakhalin Island.

Exxon Neftegas Ltd. (ENL) carried out three 4D seismic surveys during the summer of 2015. Seismic operations in two of these fields (Odoptu and Chayvo) ensonified the nearshore feeding area of Korean-Okhotsk (western) gray whales (Eschrichtius robustus), potentially disturbing feeding activities. Following model-based optimization of the source design to minimize its lateral acoustic footprint, pre-season modeling was used to compute the acoustic exposure along each survey line. Real-time acoustic data facilitated implementation of mitigation measures aimed to minimize disturbance of whales. Acoustic data originated from underwater recorders deployed on the seafloor. Two complementary approaches were used to transmit recorded sound data to a computer housed at the Central Post (CP), where decisions regarding mitigation shut downs were made. In the first approach, a limited bandwidth (2-2000 Hz) sampling of the data was transmitted via cable to a surface buoy, which relayed these data to a shore station up to 15 km away via digital VHF telemetry. At the shore station, acoustic impulses from the seismic surveys were processed to compute impulse characteristics in the form of estimates of sound exposure level and peak sound pressure level, as well as one-minute-average 1/3-octave power spectral density coefficients, which were then transmitted to the CP via the internet. In the second, the pulse characteristics were computed through algorithms running on an onboard processor in each recorder's surface buoy and sent directly to the CP computer via an Iridium satellite uplink. Both methods of data transfer proved viable, but Iridium transmission achieved the goal without the need for any shore based relay stations and is therefore more operationally efficient than VHF transmission. At the CP, analysts used the real-time acoustic data to calibrate and adjust the output of pre-season acoustical model runs. The acoustic footprint for the active seismic source, advancing synchronously with the motion of the seismic vessel and changing as the sound propagation environment changed, was computed from the calibrated and adjusted model output and integrated through the software Pythagoras with locations of gray whales provided by shore-based observers. This enabled analysts to require air gun array shutdowns before whales were exposed to mean square sound pressure levels greater than the behavioral response threshold of 163 dB re 1 µPa2. The method described here provides a realistic means of mitigating the possible effects of air guns at a behavioral response level, whereas most seismic surveys rely on pre-established mitigation radii to manage the risk of injury to a whale.

When people are defeated by artificial intelligence in a competition task requiring logical thinking, how do they make causal attribution?

Given that artificial intelligence (AI) has been predicted to eventually take on human tasks demanding logical thinking, it makes sense that we should examine psychological responses of humans when their performance is inferior to AI. Research has demonstrated that after people fail a task, whether they reorient their behavior towards success depends on what they attribute the failure to. This study investigated the causal attributions people made in a competition task requiring such thinking. We also recorded whether they wanted to re-challenge the games after they were defeated by AI. Experiments 1 (N = 74) and 2 (N = 788) recruited Japanese participants, while Experiment 3 (N = 500) comprised American participants. There were two conditions: in the first, participants competed against an AI opponent and in the other, they believed they were competing against a human. The results of the three experiments showed that participants attributed the loss to their own and their opponent's abilities more than any other factor, irrespective of the opponent type. The number of participants choosing to re-challenge the game did not differ significantly between the AI and human conditions in Experiments 1 and 3, although the number was lower in the AI condition than in the human condition in Experiment 2. Besides providing fresh insight on how people make causal attributions when competing against AI, our findings also predict how people will respond after their jobs are replaced by AI. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12144-021-02559-w.

Assessment of perceived risk and precautionary behavior toward COVID-19 pandemic using the health belief model, Saudi Arabia.

BACKGROUND: The global threat of the COVID-19 pandemic continues to evolve forming the most impactful health crises in modern history, necessities of individuals adhering to mandatory behavior change that limits the spread of the pandemic. The purpose of the current study is to identify behavioral responses of the health sciences university students during the period of the COVID-19 pandemic and determine risk perceptions using the health belief model (HBM). METHODS: A cross-sectional study using an online survey distributed among health sciences female university students in Riyadh, KSA. The questionnaire was used to assess sociodemographic characteristics; knowledge about COVID-19 and its preventive measures, risk perceptions, and beliefs using the HBM; and their actual adoption of precautionary measures. RESULTS: The mean age of 286 respondents was 21.6 years (SD 2.5). They had good knowledge, positive risk perception, and good practice. Fifty-seven percent of the respondents adhere satisfactorily to COVID-19 precautionary behavior. Respondents with positive overall risk perception had around 6 times significantly higher adherence compared to those with negative risk perception. Perceived benefits have higher odds of adherence to COVID-19 precautionary behavior. Similarly, cues to action were a significant determinant of adherence to COVID-19 precautionary behavior. CONCLUSIONS: The constructs of the HBM provided good measurement of risk perception and the respondent students had good knowledge. Yet, significant gaps were shown between COVID-19 perceived risks and the students' actual practice of personal hygienic measures, particularly hand hygiene. To put an end to the present COVID-19 and its upcoming waves, it is highly recommended to direct COVID-19 training programs specifically tailored towards university students.