The challenge of estimating global termite methane emissions.

Methane is a powerful greenhouse gas, more potent than carbon dioxide, and emitted from a variety of natural sources including wetlands, permafrost, mammalian guts and termites. As increases in global temperatures continue to break records, quantifying the magnitudes of key methane sources has never been more pertinent. Over the last 40 years, the contribution of termites to the global methane budget has been subject to much debate. The most recent estimates of termite emissions range between 9 and 15 Tg CH4 year-1, approximately 4% of emissions from natural sources (excluding wetlands). However, we argue that the current approach for estimating termite contributions to the global methane budget is flawed. Key parameters, namely termite methane emissions from soil, deadwood, living tree stems, epigeal mounds and arboreal nests, are largely ignored in global estimates. This omission occurs because data are lacking and research objectives, crucially, neglect variation in termite ecology. Furthermore, inconsistencies in data collection methods prohibit the pooling of data required to compute global estimates. Here, we summarise the advances made over the last 40 years and illustrate how different aspects of termite ecology can influence the termite contribution to global methane emissions. Additionally, we highlight technological advances that may help researchers investigate termite methane emissions on a larger scale. Finally, we consider dynamic feedback mechanisms of climate warming and land-use change on termite methane emissions. We conclude that ultimately the global contribution of termites to atmospheric methane remains unknown and thus present an alternative framework for estimating their emissions. To significantly improve estimates, we outline outstanding questions to guide future research efforts.

Community Assembly Processes of Deadwood Mycobiome in a Tropical Forest Revealed by Long-Read Third-Generation Sequencing.

Despite the importance of wood-inhabiting fungi on nutrient cycling and ecosystem functions, their ecology, especially related to their community assembly, is still highly unexplored. In this study, we analyzed the wood-inhabiting fungal richness, community composition, and phylogenetics using PacBio sequencing. Opposite to what has been expected that deterministic processes especially environmental filtering through wood-physicochemical properties controls the community assembly of wood-inhabiting fungal communities, here we showed that both deterministic and stochastic processes can highly contribute to the community assembly processes of wood-inhabiting fungi in this tropical forest. We demonstrated that the dynamics of stochastic and deterministic processes varied with wood decomposition stages. The initial stage was mainly governed by a deterministic process (homogenous selection), whereas the early and later decomposition stages were governed by the stochastic processes (ecological drift). Deterministic processes were highly contributed by wood physicochemical properties (especially macronutrients and hemicellulose) rather than soil physicochemical factors. We elucidated that fine-scale fungal-fungal interactions, especially the network topology, modularity, and keystone taxa of wood-inhabiting fungal communities, strongly differed in an initial and decomposing deadwood. This current study contributes to a better understanding of the ecological processes of wood-inhabiting fungi in tropical regions where the knowledge of wood-inhabiting fungi is highly limited.

Modelling the effects of climate and management on the distribution of deadwood in European forests.

Deadwood is a key old-growth element in European forests and a cornerstone of biodiversity conservation practices in the region, recognized as an important indicator of sustainable forest management. Despite its importance as a legacy element for biodiversity, uncertainties remain on the drivers of deadwood potentials, its spatial distribution in European forests and how it may change in the future due to management and climate change. To fill this gap, we combined a comprehensive deadwood dataset to fit a machine learning and a Bayesian hurdle-lognormal model against multiple environmental and socio-economic predictors. We deployed the models on the gridded predictors to forecast changes in deadwood volumes in Europe under alternative climate (RCP4.5 and RCP8.5) and management scenarios (biodiversity-oriented and production-oriented strategies). Our results show deadwood hotspots in montane forests of central Europe and unmanaged forests in Scandinavia. Future climate conditions may reduce deadwood potentials up to 13% under a mid-century climate, with regional losses amounting to up to 22% in Southern Europe. Nevertheless, changes in management towards more biodiversity-oriented strategies, including an increase in the share of mixed forests and extended rotation lengths, may mitigate this loss to a 4% reduction in deadwood potentials. We conclude that adaptive management can promote deadwood under changing environmental conditions and thereby support habitat maintenance and forest multifunctionality.

Trait-environment interactions of saproxylic beetles as a guide to biodiversity conservation strategies.

Conservation of biodiversity requires in-depth knowledge of trait-environment interactions to understand the influence the environment has on species assemblages. Saproxylic beetles exhibit a wide range of traits and functions in the forest ecosystems. Understanding their responses to surrounding environment thus improves our capacity to identify habitats that should be restored or protected. We investigated potential interactions between ecological traits in saproxylic beetles (feeding guilds and habitat preferences) and environmental variables (deadwood, type and age of surrounding forest). We sampled beetles from 78 plots containing newly created high stumps of Scots pine and Silver birch in boreal forest landscapes in Sweden for three consecutive years. Using a model based approach, our aim was to explore potential interactions between ecological traits and the surrounding environment at close and distant scale (20 m and 500 m radius). We found that broadleaf-preferring beetle species are positively associated with the local broadleaf-originated deadwood and broadleaf-rich forests in the surrounding landscapes. Conifer-preferring species are positively associated with the local amount of coniferous deadwood and young and old forests in the surrounding landscape. Fungivorous and predatory beetles are positively associated with old forests in the surrounding landscapes. Our results indicate that both local amounts of deadwood and types of forests in the landscape are important in shaping saproxylic beetle communities. We particularly highlight the need to increase deadwood amounts of various qualities in the landscape, exempt older forests from production and to increase broadleaf-rich habitats in order to meet different beetle species' habitat requirements. Trait responses among saproxylic beetles provide insights into the significance of broadleaf forest and dead wood as essential attributes in boreal forest restoration, which helps conservation planning and management in forest landscapes.

Assessing the potential of remote sensing-based models to predict old-growth forests on large spatiotemporal scales.

Old-growth forests provide a broad range of ecosystem services. However, due to poor knowledge of their spatiotemporal distribution, implementing conservation and restoration strategies is challenging. The goal of this study is to compare the predictive ability of socioecological factors and different sources of remotely sensed data that determine the spatiotemporal scales at which forest maturity attributes can be predicted. We evaluated various remotely sensed data that cover a broad range of spatial (from local to global) and temporal (from current to decades) extents, from Airborne Laser Scanning (ALS), aerial multispectral and stereo-imagery, Sentinel-1, Sentinel-2 and Landsat data. Using random forests, remotely sensed data were related to a forest maturity index available in 688 forest plots across four ranges of the French Alps. Each model also includes socioecological predictors related to topography, socioeconomy, pedology and climatology. We found that the different remotely sensed data provide information on the main forest structural characteristics as defined by ALS, except for Landsat, which has a too coarse resolution, and Sentinel-1, which responds differently to vegetation structure. The predictions were quite similar considering aerial remotely sensed data, on the one hand, and satellite remotely sensed data, on the other hand. Socioecological variables are the most important predictors compared to the remote sensing metrics. In conclusion, our results indicate that a wide range of remotely sensed data can be used to study old-growth forests beyond the use of ALS and despite different abilities to predict forest structure. Accounting for socioecological predictors is indispensable to avoid a significant loss of predictive accuracy. Remotely sensed data can allow for predictions to be made at different spatiotemporal resolutions and extents. This study paves the way to large-scale monitoring of forest maturity, as well as for retrospective analyses which will show to what extent predicted maturity change at different dates.

Macroclimate modulates the positive dead-wood influence on bryophyte diversity in managed and unmanaged temperate lowland forests.

Extending the network of strict forest reserves is one of the conservation measures promoted by the French National Strategy for Biodiversity. However, there is a lack of evidence concerning the efficiency of setting aside strict forest reserves to preserve biodiversity in the case of temperate forests. Moreover, there are potentially unexplored underlying ecological mechanisms that forest management could usefully mimic. In order to disentangle the respective roles of management abandonment, stand structural attributes and climatic and topographic variables in determining forest biodiversity, we conducted the first national-scale study in France comparing biodiversity in managed and in unmanaged forests. Here we focus on bryophytes (all species combined and forest specialists separately). We analyzed data from 127 plots in ten lowland forests in France. Our aim was to disentangle the relationships between bryophyte richness and (i) management abandonment per se, (ii) associated forest-structure variables like deadwood volume, and (iii) macroclimatic variables important for bryophytes (temperature, precipitation, relative humidity, solar radiation and vapor pressure deficit). For each studied combination of variables (univariate, additive or interactive models), we compared hierarchical models of several types: linear with a fixed slope, linear with a random slope, quadratic, sigmoid or threshold models. We found that deadwood variables were the main drivers of bryophyte richness in managed as well as in unmanaged stands. We observed a sigmoid relationship of total deadwood volume to overall richness, and a threshold effect of large and very large deadwood volume on forest specialist richness. The effect of management abandonment was globally non-significant, though impact varied strongly among the different forest sites. A combination of deadwood and macroclimatic variables best predicted bryophyte richness, through non-linear relationships: 1) higher solar radiation reinforced the positive effects of large deadwood on forest-specialist bryophyte richness; and 2) higher mean annual temperatures counteracted the positive effects of total deadwood amount on total bryophyte species richness. Maintaining high amounts of deadwood in both managed and unmanaged forests is likely to improve bryophyte richness and will be particularly important under ongoing climate change.

Ambient and substrate energy influence decomposer diversity differentially across trophic levels.

The species-energy hypothesis predicts increasing biodiversity with increasing energy in ecosystems. Proxies for energy availability are often grouped into ambient energy (i.e., solar radiation) and substrate energy (i.e., non-structural carbohydrates or nutritional content). The relative importance of substrate energy is thought to decrease with increasing trophic level from primary consumers to predators, with reciprocal effects of ambient energy. Yet, empirical tests are lacking. We compiled data on 332,557 deadwood-inhabiting beetles of 901 species reared from wood of 49 tree species across Europe. Using host-phylogeny-controlled models, we show that the relative importance of substrate energy versus ambient energy decreases with increasing trophic levels: the diversity of zoophagous and mycetophagous beetles was determined by ambient energy, while non-structural carbohydrate content in woody tissues determined that of xylophagous beetles. Our study thus overall supports the species-energy hypothesis and specifies that the relative importance of ambient temperature increases with increasing trophic level with opposite effects for substrate energy.

Drivers of community assembly change during succession in wood-decomposing beetle communities.

The patterns of successional change of decomposer communities is unique in that resource availability predictably decreases as decomposition proceeds. Saproxylic (i.e. deadwood-dependent) beetles are a highly diverse and functionally important decomposer group, and their community composition is affected by both deadwood characteristics and other environmental factors. Understanding how communities change with faunal succession through the decomposition process is important as this process influences terrestrial carbon dynamics. Here, we evaluate how beta-diversity of saproxylic beetle communities change with succession, as well as the effects of different major drivers of beta-diversity, such as deadwood tree species, spatial distance between locations, climate and forest structure. We studied spatial beta-diversity (i.e. dissimilarity of species composition between deadwood logs in the same year) of saproxylic beetle communities over 8 years of wood decomposition. Our study included 379 experimental deadwood logs comprising 13 different tree species in 30 forest stands in Germany. We hypothesized that the effects of tree species dissimilarity, measured by phylogenetic distance, and climate on beta-diversity decrease over time, while the effects of spatial distance between logs and forest structure increase. Observed beta-diversity of saproxylic beetle communities increased over time, whereas standardized effects sizes (SES; based on null models) of beta-diversity decreased indicating higher beta-diversity than expected during early years. Beta-diversity increased with increasing phylogenetic distance between tree species and spatial distance among regions, and to a lesser extent with spatial distance within regions and differences in climate and forest structure. Whereas effects of space, climate and forest structure were constant over time, the effect of phylogenetic distance decreased. Our results show that the strength of the different drivers of saproxylic beetle community beta-diversity changes along deadwood succession. Beta-diversity of early decay communities was strongly associated with differences among tree species. Although this effect decreased over time, beta-diversity remained high throughout succession. Possible explanations for this pattern include differences in decomposition rates and fungal communities between logs or the priority effect of early successional communities. Our results suggest that saproxylic beetle diversity can be enhanced by promoting forests with diverse tree communities and structures.

Forest gaps increase true bug diversity by recruiting open land species.

Forests canopy gaps play an important role in forest ecology by driving the forest mosaic cycle and creating conditions for rapid plant reproduction and growth. The availability of young plants, which represent resources for herbivores, and modified environmental conditions with greater availability of light and higher temperatures, promote the colonization of animals. Remarkably, the role of gaps on insect communities has received little attention and the source of insects colonizing gaps has not been studied comprehensively. Using a replicated full-factorial forest experiment (treatments: Gap; Gap + Deadwood; Deadwood; Control), we show that following gap creation, there is a rapid change in the true bug (Heteroptera) community structure, with an increase in species that are mainly recruited from open lands. Compared with closed-canopy treatments (Deadwood and Control), open canopy treatments (Gap and Gap + Deadwood) promoted an overall increase in species (+ 59.4%, estimated as number of species per plot) and individuals (+ 76.3%) of true bugs, mainly herbivores and species associated to herbaceous vegetation. Community composition also differed among treatments, and all 17 significant indicator species (out of 117 species in total) were associated with the open canopy treatments. Based on insect data collected in grasslands and forests over an 11-year period, we found that the species colonizing experimental gaps had greater body size and a greater preference for open vegetation. Our results indicate that animal communities that assemble following gap creation contain a high proportion of habitat generalists that not occurred in closed forests, contributing significantly to overall diversity in forest mosaics.

How decaying wood affects the accumulation of polycyclic aromatic hydrocarbons in soil of temperate mountain forest.

The aim of our research was to determine the role of heavily decomposed deadwood in the shaping of accumulation of polycyclic aromatic hydrocarbons (PAHs) in mountain forest ecosystems. The study included heavily decomposed spruce wood located in lower and higher mountain locations (600 and 1200 m a.s.l.) and at the south (S) and north (N) exposure. The accumulation of PAHs in deadwood was compared to the accumulation of PAHs in surface soil horizons in the immediate vicinity of decaying wood and in soils unaffected by decaying wood. Basic chemical properties and enzymatic activity were determined in wood and soil samples. The conducted research indicates the importance of decaying wood for the formation of PAHs accumulation in mountain forest soils. The lowest content of PAHs was recorded in samples of heavily decomposed wood, which at the same time was characterized by the highest enzymatic activity. Significantly higher PAHs content was recorded in soils unaffected by components released from decaying wood. Our research confirmed the importance of location conditions (exposure, altitude) in shaping PAHs accumulation. The highest mountain locations (1200 m a.s.l.) to N exposure were characterized by the highest accumulation of PAHs.

Cross-kingdom interactions and functional patterns of active microbiota matter in governing deadwood decay.

Microbial community members are the primary microbial colonizers and active decomposers of deadwood. This study placed sterilized standardized beech and spruce sapwood specimens on the forest ground of 8 beech- and 8 spruce-dominated forest sites. After 370 days, specimens were assessed for mass loss, nitrogen (N) content and 15N isotopic signature, hydrolytic and lignin-modifying enzyme activities. Each specimen was incubated with bromodeoxyuridine (BrdU) to label metabolically active fungal and bacterial community members, which were assessed using amplicon sequencing. Fungal saprotrophs colonized the deadwood accompanied by a distinct bacterial community that was capable of cellulose degradation, aromatic depolymerization, and N2 fixation. The latter were governed by the genus Sphingomonas, which was co-present with the majority of saprotrophic fungi regardless of whether beech or spruce specimens were decayed. Moreover, the richness of the diazotrophic Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium group was significantly correlated with mass loss, N content and 15N isotopic signature. By contrast, presence of obligate predator Bdellovibrio spp. shifted bacterial community composition and were linked to decreased beech deadwood decay rates. Our study provides the first account of the composition and function of metabolically active wood-colonizing bacterial and fungal communities, highlighting cross-kingdom interactions during the early and intermediate stages of wood decay.

Living, dead, and absent trees-How do moth outbreaks shape small-scale patterns of soil organic matter stocks and dynamics at the Subarctic mountain birch treeline?

Mountain birch forests (Betula pubescens Ehrh. ssp. czerepanovii) at the subarctic treeline not only benefit from global warming, but are also increasingly affected by caterpillar outbreaks from foliage-feeding geometrid moths. Both of these factors have unknown consequences on soil organic carbon (SOC) stocks and biogeochemical cycles. We measured SOC stocks down to the bedrock under living trees and under two stages of dead trees (12 and 55 years since moth outbreak) and treeless tundra in northern Finland. We also measured in-situ soil respiration, potential SOC decomposability, biological (enzyme activities and microbial biomass), and chemical (N, mineral N, and pH) soil properties. SOC stocks were significantly higher under living trees (4.1 ± 2.1 kg m²) than in the treeless tundra (2.4 ± 0.6 kg m²), and remained at an elevated level even 12 (3.7 ± 1.7 kg m²) and 55 years (4.9 ± 3.0 kg m²) after tree death. Effects of tree status on SOC stocks decreased with increasing distance from the tree and with increasing depth, that is, a significant effect of tree status was found in the organic layer, but not in mineral soil. Soil under living trees was characterized by higher mineral N contents, microbial biomass, microbial activity, and soil respiration compared with the treeless tundra; soils under dead trees were intermediate between these two. The results suggest accelerated organic matter turnover under living trees but a positive net effect on SOC stocks. Slowed organic matter turnover and continuous supply of deadwood may explain why SOC stocks remained elevated under dead trees, despite the heavy decrease in aboveground C stocks. We conclude that the increased occurrence of moth damage with climate change would have minor effects on SOC stocks, but ultimately decrease ecosystem C stocks (49% within 55 years in this area), if the mountain birch forests will not be able to recover from the outbreaks.

Agroforestry perennials reduce nitrous oxide emissions and their live and dead trees increase ecosystem carbon storage.

Agroforestry systems (AFS) contribute to carbon (C) sequestration and reduction in greenhouse gas emissions from agricultural lands. However, previously understudied differences among AFS may underestimate their climate change mitigation potential. In this 3-year field study, we assessed various C stocks and greenhouse gas emissions across two common AFS (hedgerows and shelterbelts) and their component land uses: perennial vegetated areas with and without trees (woodland and grassland, respectively), newly planted saplings in grassland, and adjacent annual cropland in central Alberta, Canada. Between 2018 and 2020 (~April-October), nitrous oxide emissions were 89% lower under perennial vegetation relative to the cropland (0.02 and 0.18 g N m-2  year-1 , respectively). In 2020, heterotrophic respiration in the woodland was 53% lower in shelterbelts relative to hedgerows (279 and 600 g C m-2  year-1 , respectively). Within the woodland, deadwood C stock was particularly important in hedgerows (35 Mg C ha-1 or 7% of ecosystem C) relative to shelterbelts (2 Mg C ha-1 or <1% of ecosystem C), and likely affected C cycling differences between the woodland types by enhancing soil labile C and microbial biomass in hedgerows. Deadwood C stock was positively correlated with annual heterotrophic respiration and total (to ~100 cm depth) soil organic C, water-soluble organic C, and microbial biomass C. Total ecosystem C was 1.90-2.55 times greater within the woodland than all other land uses, with 176, 234, 237, and 449 Mg C ha-1 found in the cropland, grassland, planted saplings treatment, and woodland, respectively. Shelterbelt and hedgerow woodlands contained 2.09 and 3.03 times more C, respectively, than adjacent cropland. Our findings emphasize the importance of AFS for fostering C sequestration and reducing greenhouse gas emissions and, in particular, retaining hedgerows (legacy woodland) and their associated deadwood across temperate agroecosystems will help mitigate climate change.

Natural disturbance regimes as a guide for sustainable forest management in Europe.

In Europe, forest management has controlled forest dynamics to sustain commodity production over multiple centuries. Yet over-regulation for growth and yield diminishes resilience to environmental stress as well as threatens biodiversity, leading to increasing forest susceptibility to an array of disturbances. These trends have stimulated interest in alternative management systems, including natural dynamics silviculture (NDS). NDS aims to emulate natural disturbance dynamics at stand and landscape scales through silvicultural manipulations of forest structure and landscape patterns. We adapted a "Comparability Index" (CI) to assess convergence/divergence between natural disturbances and forest management effects. We extended the original CI concept based on disturbance size and frequency by adding the residual structure of canopy trees after a disturbance as a third dimension. We populated the model by compiling data on natural disturbance dynamics and management from 13 countries in Europe, covering four major forest types (i.e., spruce, beech, oak, and pine-dominated forests). We found that natural disturbances are highly variable in size, frequency, and residual structure, but European forest management fails to encompass this complexity. Silviculture in Europe is skewed toward even-aged systems, used predominately (72.9% of management) across the countries assessed. The residual structure proved crucial in the comparison of natural disturbances and silvicultural systems. CI indicated the highest congruence between uneven-aged silvicultural systems and key natural disturbance attributes. Even so, uneven-aged practices emulated only a portion of the complexity associated with natural disturbance effects. The remaining silvicultural systems perform poorly in terms of retention compared to tree survivorship after natural disturbances. We suggest that NDS can enrich Europe's portfolio of management systems, for example where wood production is not the primary objective. NDS is especially relevant to forests managed for habitat quality, risk reduction, and a variety of ecosystem services. We suggest a holistic approach integrating NDS with more conventional practices.

Negative effects of forest gaps on dung removal in a full-factorial experiment.

Ecosystem functioning may directly or indirectly-via change in biodiversity-respond to land use. Dung removal is an important ecosystem function central for the decomposition of mammal faeces, including secondary seed dispersal and improved soil quality. Removal usually increases with dung beetle diversity and biomass. In forests, dung removal can vary with structural variables that are, however, often interrelated, making experiments necessary to understand the role of single variables on ecosystem functions. How gaps and deadwood, two main outcomes of forest management influence dung removal, is unknown. We tested if dung removal responds to gap creation and deadwood provisioning or if treatment effects are mediated via responses of dung beetles. We expected lower removal rates in gaps due to lower dung beetle biomass and diversity. We sampled dung beetles and measured dung removal in a highly-replicated full-factorial forest experiment established at 29 sites in three regions of Germany (treatments: Gap, Gap + Deadwood, Deadwood, Control). All gaps were experimentally created and had a diameter of around 30 m. Dung beetle diversity, biomass and dung removal were each lower in gaps than in controls. Dung removal decreased from 61.9% in controls to 48.5% in gaps, irrespective of whether or not the gap had deadwood. This treatment effect was primarily driven by dung beetle biomass but not diversity. Furthermore, dung removal was reduced to 56.9% in the deadwood treatment. Our findings are not consistent with complementarity effects of different dung beetle species linked to biodiversity-ecosystem functioning relationships that have been shown in several ecosystems. In contrast, identity effects can be pronounced: gaps reduced the abundance of a large-bodied key forest species (Anoplotrupes stercorosus), without compensatory recruitment of open land species. While gaps and deadwood are important for many forest organisms, dung beetles and dung removal respond negatively. Our results exemplify how experiments can contribute to test hypotheses on the interrelation between land use, biodiversity and ecosystem functioning.

Beetle diversity is higher in sunny forests due to higher microclimatic heterogeneity in deadwood.

Microclimate is a crucial driver of saproxylic beetle assemblages, with more species often found in sunny forests than in shady ones. Whether this pattern is caused by a higher detectability due to increased beetle activity under sunny conditions or a greater diversity of beetles emerging from sun-exposed deadwood remains unclear. This study examined whether sun exposure leads to higher microclimatic heterogeneity in deadwood and whether this drives beetle diversity in deadwood logs and at forest stand scale. Saproxylic beetles were sampled at the stand scale using flight-interception traps and at object scale using stem-emergence traps on deadwood logs at the same site. The variability in wood surface temperature was measured on single logs and between logs as a proxy for microclimatic heterogeneity in deadwood. Abundance in sunny forests was higher at the stand scale, and in shady forests at the object scale. The estimated number of species was higher in sunny forests at both scales and correlated positively with temperature variability on single logs and between logs at the stand scale and, albeit weakly, with temperature variability on single logs at the object scale. Gamma-diversity, and thus beta-diversity, across logs at the object scale was higher in sunny forests. These findings indicate that sun exposure promotes saproxylic beetle diversity due to higher microclimatic heterogeneity within and between deadwood logs. Our study therefore corroborates previous research demonstrating the importance of canopy cover and microclimate for forest biodiversity.

Aesthetic preferences for deadwood in forest landscape: A case study in Italy.

In the last decades, the structural and functional role of standing dead trees and lying deadwood in forests has been widely recognized by scientific community and forest managers. However, a large amount of deadwood in forests can have negative impacts on recreational forests by reducing the aesthetic value and site attractiveness. The aims of the present study are to investigate whether deadwood in forests is truly perceived negatively by people and whether socio-demographic characteristics influence the respondents' perception. To achieve these aims, the study was implemented by submitting an online questionnaire to a sample of 1292 Italian citizens. The results show that 73.4% of respondents have previous knowledge of the concept of deadwood in forests, while 26.6% have never heard this concept. For most of the respondents, standing dead trees and lying deadwood have a negative aesthetic effect on the landscape (52.2% and 42.9%), while for only 7.5% and 23.0% of respondents standing dead trees and lying deadwood have a positive effect on forest landscape. The results show that for all six forest stands proposed (Old European beech coppice, Mediterranean maquis, Norway spruce high forest, simple sweet Chestnut coppice, European beech high forest, black pine high forest) the respondents prefer the situation without deadwood. Finally, the results show that deadwood - both standing dead trees and lying deadwood - in forests is on average more appreciated by male (rather than female), young people (rather than old people), and people with a low level of education (rather than people with a high level of education).

What does a threatened saproxylic beetle look like? Modelling extinction risk using a new morphological trait database.

The extinction of species is a non-random process, and understanding why some species are more likely to go extinct than others is critical for conservation efforts. Functional trait-based approaches offer a promising tool to achieve this goal. In forests, deadwood-dependent (saproxylic) beetles comprise a major part of threatened species, but analyses of their extinction risk have been hindered by the availability of suitable morphological traits. To better understand the mechanisms underlying extinction in insects, we investigated the relationships between morphological features and the extinction risk of saproxylic beetles. Specifically, we hypothesised that species darker in colour, with a larger and rounder body, a lower mobility, lower sensory perception and more robust mandibles are at higher risk. We first developed a protocol for morphological trait measurements and present a database of 37 traits for 1,157 European saproxylic beetle species. Based on 13 selected, independent traits characterising aspects of colour, body shape, locomotion, sensory perception and foraging, we used a proportional-odds multiple linear mixed-effects model to model the German Red List categories of 744 species as an ordinal index of extinction risk. Six out of 13 traits correlated significantly with extinction risk. Larger species as well as species with a broad and round body had a higher extinction risk than small, slim and flattened species. Species with short wings had a higher extinction risk than those with long wings. On the contrary, extinction risk increased with decreasing wing load and with higher mandibular aspect ratio (shorter and more robust mandibles). Our study provides new insights into how morphological traits, beyond the widely used body size, determine the extinction risk of saproxylic beetles. Moreover, our approach shows that the morphological characteristics of beetles can be comprehensively represented by a selection of 13 traits. We recommend them as a starting point for functional analyses in the rapidly growing field of ecological and conservation studies of deadwood.

Effects of disturbance patterns and deadwood on the microclimate in European beech forests.

More frequent and severe disturbances increasingly open the forest canopy and initiate tree regeneration. Simultaneously, increasing weather extremes, such as drought and heat, are threatening species adapted to cool and moist climate. The magnitude of the microclimatic buffering capacity of forest canopies to mitigate hot and dry weather conditions and its disturbance-induced reduction remains poorly quantified. Also, the influence of disturbance legacies (e.g., deadwood) on forest microclimate is unresolved. In a unique manipulation experiment we investigated (i) the microclimatic buffering capacity of forest canopies in years with different climatic conditions; (ii) the impacts of spatial disturbance patterns on surface light and microclimate; and (iii) the effect of deadwood presence and type on microclimate. Treatments included two disturbance patterns (i.e., aggregated and distributed), four deadwood types (i.e., standing, downed, standing and downed, removed), and one untreated control (i.e., nine treatments in total), replicated at five sites dominated by European beech (Fagus sylvatica L.) in southeastern Germany. We measured forest floor light conditions and derived diurnal extremes and variation in temperature (T) and vapor pressure deficit (VPD) during four consecutive summer seasons (2016 - 2019). The buffering capacity of intact forest canopies was higher in warm and dry years. Surface light was significantly higher in spatially aggregated disturbance gaps compared to distributed disturbances of similar severity. An increase in surface light by 10 % relative to closed canopies elevated Tmax and VPDmax by 0.42°C and 0.04 kPa, respectively. Deadwood presence and type did not affect the forest microclimate significantly. Microclimatic buffering under forest canopies can dampen the effects of climate change. However, increasing canopy disturbances result in more light penetrating the canopy, reducing the microclimatic buffering capacity of forests. We conclude that forest management should foster microclimatic buffering in forests as one element of a multi-pronged strategy to counter climate change.

Home-Field Advantage in Wood Decomposition Is Mainly Mediated by Fungal Community Shifts at "Home" Versus "Away".

The home-field advantage (HFA) hypothesis has been used intensively to study leaf litter decomposition in various ecosystems. However, the HFA in woody substrates is still unexplored. Here, we reanalyzed and integrated existing datasets on various groups of microorganisms collected from natural deadwood of two temperate trees, Fagus sylvatica and Picea abies, from forests in which one or other of these species dominates but where both are present. Our aims were (i) to test the HFA hypothesis on wood decomposition rates of these two temperate tree species, and (ii) to investigate if HFA hypothesis can be explained by diversity and community composition of bacteria and in detail N-fixing bacteria (as determined by molecular 16S rRNA and nifH gene amplification) and fungi (as determined by molecular ITS rRNA amplification and sporocarp surveys). Our results showed that wood decomposition rates were accelerated at "home" versus "away" by 38.19% ± 20.04% (mean ± SE). We detected strong changes in fungal richness (increase 36-50%) and community composition (RANOSIM = 0.52-0.60, P < 0.05) according to HFA hypothesis. The changes of fungi were much stronger than for total bacteria and nitrogen fixing for both at richness and community composition levels. In conclusion, our results support the HFA hypothesis in deadwood: decomposition rate is accelerated at home due to specialization of fungal communities produced by the plant community above them. Furthermore, the higher richness of fungal sporocarps and nitrogen-fixing bacteria (nifH) may stimulate or at least stabilize wood decomposition rates at "home" versus "away."