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Rarity, range restriction, and narrow endemism tend to carry dire and urgent conservation implications for imperilled species. What is also clear is that human-associated extinction risk factors such as urbanization and deforestation pose overwhelming threats to range-restricted species. In this issue of Molecular Ecology, Antunes et al. (2022) demonstrate that these threats can also impact widespread species. By comparing newts in the genus Lissotriton that co-occur in the same geographical region, they expose the distinctness of risks facing species with different habitat preferences. Their study emphasizes the importance of local-scale landscape genetics to reveal the nuances of population connectivity that might otherwise be missed by studying a broader spatial scale.
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Conservación de los Recursos Naturales , Ecosistema , Humanos , Ecología , Dinámica Poblacional , UrbanizaciónRESUMEN
Forest crimes are among the serious threats destroying forests. To prevent the forest crimes there are various solutions proposed, such as fortification of the laws, increasing the penalties, or increasing the public awareness. This article, however, suggests an alternative solution of preventing the forest crimes by investigating the relations between the individual financial supports provided to forest villagers and the levels of various forest crime types in Turkey. The study shows that, when the forest villagers are given financial supports, the levels of illegal logging, illegal transferring of forest products, illegal expansion of private lands into forests, illegal processing of trees, and illegal pasturage crimes decrease significantly. However, the financial supports do not affect the levels of illegal occupation of forestlands crime.
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Conservación de los Recursos Naturales , Agricultura Forestal , Bosques , Árboles , CrimenRESUMEN
PREMISE: Reconciling the use of taxonomy to partition morphological variation and describe genetic divergence within and among closely related species is a persistent challenge in phylogenetics. We reconstructed phylogenetic relationships among Cedrela odorata (Meliaceae) and five closely allied species to test the genetic basis for the current model of species delimitation in this economically valuable and threatened genus. METHODS: We prepared a nuclear species tree with the program SNPhylo and 16,000 single-nucleotide polymorphisms from 168 Cedrela specimens. Based on clades present and ancestral patterns ADMIXTURE, we designed nine species delimitation models and compared each model to current taxonomy with Bayes factor delimitation. Timing of major lineage divergences was estimated with the program SNAPP. RESULTS: The resulting analysis revealed that modern C. odorata evolved from two genetically distinct ancestral sources. All species delimitation models tested better fit the data than the model representing current taxonomic delimitation. Models with the greatest marginal likelihoods separated Mesoamerican C. odorata and South American C. odorata into two species and lumped C. angustifolia and C. montana as a single species. We estimated that Cedrela diversified in South America within the last 19 million years following one or more dispersal events from Mesoamerican lineages. CONCLUSIONS: Our analyses show that the present taxonomic understanding within the genus obscures divergent lineages in C. odorata due in part to morphological differentiation and taxonomic distinctions that are not predictably associated with genetic divergence. A more accurate application of taxonomy to C. odorata and related species may aid in its conservation, management, and restoration efforts.
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Cedrela , Cedrela/genética , Cedrela/anatomía & histología , Filogenia , Teorema de Bayes , Especificidad de la Especie , América del SurRESUMEN
All walks of life have been affected by COVID-19 but smallholders from developing countries have been impacted more than others as they are heavily reliant on forest and agriculture for their livelihoods and have limited capacity to deal with COVID-19. Scholars are heavily engaged in assessing the impacts of COVID-19 on health and wellbeing, gender, food production and supply, stock market and the overall economy but not on the forestry sector. Using questionnaire surveys and key informant interviews-informed by grey literature and published articles- representing Division Forest Offices, Provincial Forest Directorates, and the Ministry of Forests and Environment in Nepal, this study assessed the impact of COVID-19 on the forestry sector of Nepal. Our analysis suggests that: (1) nature-based tourism is more severely affected than other sectors; (2) private, religious and leasehold forests faced minimal impacts of COVID-19 than that of community and government-managed forests; (3) wild boar (Sus scrofa), different species of deer, and birds have been more impacted than other wild animals; (4) the price of the timber has increased significantly whereas the price of non-timber forests products (NTFPs) has decreased; and (5) illegal logging and poaching have increased but the incidence of forest encroachment has been reduced. Our study further reveals that agroforestry practices in home gardens, borrowing money from neighbors/banks/landlords and liquidating livestock remained key alternatives for smallholders during COVID-19. Many studies reported that reverse migration could create chaos in Nepal, but our study suggests that it may enhance rural innovation and productivity, as returnees may use their acquired knowledge and skills to develop new opportunities. As COVID-19 has created a war-like situation worldwide, Nepal should come up with a forward-looking fiscal response with alternative income generation packages to local living to counter the impacts of COVID-19 on the forestry sector. One of the options could be implementing similar programs to that of India's US$ 800 Compensatory Afforestation Program and Pakistan's 10 Billion Tree Tsunami Program, which will create a win-win situation, i.e., generate employment for reverse migrants and promotes forest restoration.
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Tropical peat swamp forests (PSFs) are globally important carbon stores under threat. In Southeast Asia, 35% of peatlands had been drained and converted to plantations by 2010, and much of the remaining forest had been logged, contributing significantly to global carbon emissions. Yet, tropical forests have the capacity to regain biomass quickly and forests on drained peatlands may grow faster in response to soil aeration, so the net effect of humans on forest biomass remains poorly understood. In this study, two lidar surveys (made in 2011 and 2014) are compared to map forest biomass dynamics across 96 km2 of PSF in Kalimantan, Indonesia. The peatland is now legally protected for conservation, but large expanses were logged under concessions until 1998 and illegal logging continues in accessible portions. It was hypothesized that historically logged areas would be recovering biomass while recently logged areas would be losing biomass. We found that historically logged forests were recovering biomass near old canals and railways used by the concessions. Lidar detected substantial illegal logging activity-579 km of logging canals were located beneath the canopy. Some patches close to these canals have been logged in the 2011-2104 period (i.e. substantial biomass loss) but, on aggregate, these illegally logged regions were also recovering. Unexpectedly, rapid growth was also observed in intact forest that had not been logged and was over a kilometre from the nearest known canal, perhaps in response to greater aeration of surface peat. Comparing these results with flux measurements taken at other nearby sites, we find that carbon sequestration in above-ground biomass may have offset roughly half the carbon efflux from peat oxidation. This study demonstrates the power of repeat lidar survey to map fine-scale forest dynamics in remote areas, revealing previously unrecognized impacts of anthropogenic global change.
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Suelo , Humedales , Asia Sudoriental , Bosques , Humanos , Indonesia , Encuestas y Cuestionarios , Clima TropicalRESUMEN
MAIN CONCLUSION: ITS2+ trnH - psbA was the best combination of DNA barcode to resolve the Dalbergia wood species studied. We demonstrate the feasibility of building a DNA barcode reference database using xylarium wood specimens. The increase in illegal logging and timber trade of CITES-listed tropical species necessitates the development of unambiguous identification methods at the species level. For these methods to be fully functional and deployable for law enforcement, they must work using wood or wood products. DNA barcoding of wood has been promoted as a promising tool for species identification; however, the main barrier to extensive application of DNA barcoding to wood is the lack of a comprehensive and reliable DNA reference library of barcodes from wood. In this study, xylarium wood specimens of nine Dalbergia species were selected from the Wood Collection of the Chinese Academy of Forestry and DNA was then extracted from them for further PCR amplification of eight potential DNA barcode sequences (ITS2, matK, trnL, trnH-psbA, trnV-trnM1, trnV-trnM2, trnC-petN, and trnS-trnG). The barcodes were tested singly and in combination for species-level discrimination ability by tree-based [neighbor-joining (NJ)] and distance-based (TaxonDNA) methods. We found that the discrimination ability of DNA barcodes in combination was higher than any single DNA marker among the Dalbergia species studied, with the best two-marker combination of ITS2+trnH-psbA analyzed with NJ trees performing the best (100% accuracy). These barcodes are relatively short regions (<350 bp) and amplification reactions were performed with high success (≥90%) using wood as the source material, a necessary factor to apply DNA barcoding to timber trade. The present results demonstrate the feasibility of using vouchered xylarium specimens to build DNA barcoding reference databases.
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Código de Barras del ADN Taxonómico/métodos , Dalbergia/clasificación , Dalbergia/genética , Especies en Peligro de Extinción , Estudios de Factibilidad , Marcadores Genéticos/genética , Especificidad de la Especie , Madera/clasificación , Madera/genéticaRESUMEN
Maintaining human cooperation in the context of common-pool resource management is extremely important because otherwise we risk overuse and corruption. To analyse the interplay between economic and ecological factors leading to corruption, we couple the resource dynamics and the evolutionary dynamics of strategic decision making into a powerful analytical framework. The traits of this framework are: (i) an arbitrary number of harvesters share the responsibility to sustainably exploit a specific part of an ecosystem, (ii) harvesters face three strategic choices for exploiting the resource, (iii) a delegated enforcement system is available if called upon, (iv) enforcers are either honest or corrupt, and (v) the resource abundance reflects the choice of harvesting strategies. The resulting dynamical system is bistable; depending on the initial conditions, it evolves either to cooperative (sustainable exploitation) or defecting (overexploitation) equilibria. Using the domain of attraction to cooperative equilibria as an indicator of successful management, we find that the more resilient the resource (as implied by a high growth rate), the more likely the dominance of corruption which, in turn, suppresses the cooperative outcome. A qualitatively similar result arises when slow resource dynamics relative to the dynamics of decision making mask the benefit of cooperation. We discuss the implications of these results in the context of managing common-pool resources.
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Conservación de los Recursos Naturales , Teoría del Juego , Conducta Social , Evolución Biológica , Conducta Cooperativa , Humanos , Modelos TeóricosRESUMEN
This review synthesizes the literature studying illegality and government corruption in forest management. After discussing the theoretical connections between different types of corruption and illegal forest-related activities it describes the major trends in previous studies, examining cross-national patterns as well as local in-depth studies. Both theory and available empirical findings provide a straightforward suggestion: Bribery is indeed a "door opener" for illegal activities to take place in forest management. It then discusses the implications for conservation, focusing first on international protection schemes such as the REDD+ and second on efforts to reduce illegality and bribery in forest management. Key aspects to consider in the discussion on how to design monitoring institutions of forest regulations is how to involve actors without the incentive to engage in bribery and how to make use of new technologies that may publicize illegal behavior in distant localities. The review concludes by discussing avenues for future research.
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Conservación de los Recursos Naturales , Agricultura Forestal , Conservación de los Recursos Naturales/legislación & jurisprudencia , Conducta Criminal , Agricultura Forestal/legislación & jurisprudencia , Agricultura Forestal/organización & administración , Bosques , GobiernoRESUMEN
We used aerial photographs, satellite images, and field surveys to monitor forest cover in the core zones of the Monarch Butterfly Biosphere Reserve in Mexico from 2001 to 2012. We used our data to assess the effectiveness of conservation actions that involved local, state, and federal authorities and community members (e.g., local landowners and private and civil organizations) in one of the world's most iconic protected areas. From 2001 through 2012, 1254 ha were deforested (i.e., cleared areas had <10% canopy cover), 925 ha were degraded (i.e., areas for which canopy forest decreased), and 122 ha were affected by climatic conditions. Of the total 2179 ha of affected area, 2057 ha were affected by illegal logging: 1503 ha by large-scale logging and 554 ha by small-scale logging. Mexican authorities effectively enforced efforts to protect the monarch reserve, particularly from 2007 to 2012. Those efforts, together with the decade-long financial support from Mexican and international philanthropists and businesses to create local alternative-income generation and employment, resulted in the decrease of large-scale illegal logging from 731 ha affected in 2005-2007 to none affected in 2012, although small-scale logging is of growing concern. However, dire regional social and economic problems remain, and they must be addressed to ensure the reserve's long-term conservation. The monarch butterfly (Danaus plexippus) overwintering colonies in Mexico-which engage in one of the longest known insect migrations-are threatened by deforestation, and a multistakeholder, regional, sustainable-development strategy is needed to protect the reserve.
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Mariposas Diurnas/fisiología , Conservación de los Recursos Naturales/tendencias , Ambiente , Monitoreo del Ambiente , Animales , Conservación de los Recursos Naturales/economía , México , Estaciones del AñoRESUMEN
The use of genetic data for timber species and population assignment is a powerful tool for combating the illegal timber trade, but the challenges of extracting DNA from timber have prevented the routine use of genetics as a supply chain management tool. To overcome these challenges, we explored the feasibility of focused ultrasound extraction (FUSE) for rapid DNA release from timber. Using high-pressure ultrasound pulses, FUSE generates a cavitation bubble cloud that disintegrates samples into acellular debris, resulting in the mechanical release of DNA. In this work, FUSE was applied to white oak (Quercus alba) timber shavings to test the feasibility of using FUSE for timber DNA extraction for the first time. Results showed that FUSE processing disintegrated the tissue samples and released significant quantities of DNA. After five minutes of tissue processing DNA quantities of 0.21 ± 0.02â¯ng/mg, 0.99 ± 0.32â¯ng/mg, and 0.14 ± 0.01â¯ng/mg, were released from medium, coarse, and combination shaving groups, respectively. Amplification and sequencing of regions within the matK and rbcL chloroplast genes confirmed that the quality of DNA prepared with FUSE was suitable for PCR and short-read sequencing applications. Overall, these results show that FUSE can serve as a DNA sample preparation method capable of releasing high-quality DNA from timber in a fraction of the time required by conventional extraction methods. Based on the improved efficiency of DNA release with FUSE, ongoing work aims to develop this technology into portable systems that can be used to rapidly prepare timber samples for genetic species identification.
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ADN de Plantas , Reacción en Cadena de la Polimerasa , Quercus , ADN de Plantas/genética , Quercus/genética , Análisis de Secuencia de ADN , Especificidad de la Especie , Dermatoglifia del ADN , Madera , Ultrasonido , Manejo de Especímenes/métodos , Estudios de FactibilidadRESUMEN
The illicit exploitation of Brazilwood (Paubrasilia echinata) presents a significant challenge in Brazil, given its substantial value in the global production of bows for musical instruments. To address timber provenance, the use of strontium (Sr) isotope ratios as indicators of bedrock signatures has emerged as a robust tool in forensic investigations. In this study, we critically evaluate the efficacy of this approach using Sr isotope data derived from bulk soils and trees collected at two distinct sites in Brazil. Despite the statistically indistinguishable 87Sr/86Sr ratios observed in the investigated tree species, the compiled 87Sr/86Sr isotope ratios of Brazilwood from Brazilwood National Park (PNPB) and the ES Group provide valuable insights into the potential application of this method for tracing forensic timber seizures. This pilot study also addresses crucial sampling considerations. While the regional signatures exhibit clear distinctions, the limited sample sizes underscore the necessity for supplementary methods to confidently attribute timber to a specific source forest. In isolation, this method proves most effective in refuting presumed timber provenances rather than definitively confirming them. The discussion delves into the nuances of the Sr isotope data, emphasizing the importance of increasing the number of samples and exploring complementary techniques for a more comprehensive and reliable assessment of timber origin.
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Extreme difficulties in species identification of illegally sourced wood with conventional tools have accelerated illicit logging activities, leading to the destruction of natural resources in India. In this regard, the study primarily focused on developing a DNA barcode database for 41 commercial timber tree species which are highly vulnerable to adulteration in south India. The developed DNA barcode database was validated using an integrated approach involving wood anatomical features of traded wood samples collected from south India. Traded wood samples were primarily identified using wood anatomical features using IAWA list of microscopic features for hardwood identification. Consortium of Barcode of Life (CBOL) recommended barcode gene regions (rbcL, matK & psbA-trnH) were employed for developing DNA barcode database. Secondly, we employed artificial intelligence (AI) analytical platform, Waikato Environment for Knowledge Analysis (WEKA) for analyzing DNA barcode sequence database which could append precision, speed, and accuracy for the entire identification process. Among the four classification algorithms implemented in the machine learning algorithm (WEKA), best performance was shown by SMO, which could clearly allocate individual samples to their respective sequence database of biological reference materials (BRM) with 100 % accuracy, indicating its efficiency in authenticating the traded timber species. Major advantage of AI is the ability to analyze huge data sets with more precision and also provides a large platform for rapid authentication of species, which subsequently reduces human labor and time. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03604-0.
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The increasing use of plant evidence in forensic investigations gave rise to a powerful new discipline - Forensic Botany - that analyses micro- or macroscopic plant materials, such as the totality or fragments of an organ (i.e., leaves, stems, seeds, fruits, roots) and tissue (i.e., pollen grains, spores, fibers, cork) or its chemical composition (i. e., secondary metabolites, isotopes, DNA, starch grains). Forensic botanists frequently use microscopy, chemical analysis, and botanical expertise to identify and interpret evidence crucial to solving civil and criminal issues, collaborating in enforcing laws or regulations, and ensuring public health safeguards. The present work comprehensively examines the current state and future potential of Forensic Botany. The first section conveys the critical steps of plant evidence collection, documentation, and preservation, emphasizing the importance of these initial steps in maintaining the integrity of the items. It explores the different molecular analyses, covering the identification of plant species and varieties or cultivars, and discusses the limitations and challenges of these techniques in forensics. The subsequent section covers the diversity of Forensic Botany approaches, examining how plant evidence exposes food and pharmaceutical frauds, uncovers insufficient or erroneous labeling, traces illegal drug trafficking routes, and combats the illegal collection or trade of protected species and derivatives. National and global security issues, including the implications of biological warfare, bioterrorism, and biocrime are addressed, and a review of the contributions of plant evidence in crime scene investigations is provided, synthesizing a comprehensive overview of the diverse facets of Forensic Botany.
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Botánica , Plantas , Plantas/genética , Medicina Legal/métodos , Polen , SemillasRESUMEN
A non-targeted metabolomics-based approach using liquid chromatography high-resolution mass spectrometry was used to authenticate spruce wood (Picea abies) from two geographic source areas. The two sample sites were located in Germany and only 250â¯km apart. In order to achieve the highest possible metabolite coverage, the spruces samples were measured with four different methods using liquid chromatography high-resolution mass spectrometry. In this way, a total of approximately 4,100 features were detected, which included non-polar, polar, and intermediate-polar metabolites. Using supervised multivariate methods, a distinction between the two sample groups could be achieved on the basis of non-polar data sets. The major metabolites contributing to differentiation were identified by MS/MS experiments and were from the following classes of compounds: ceramides, fatty acids, glycerolipids, and phytosterols. Based on the soil descriptions of the two sites, it was concluded that there is probably a close relationship between nutrient availability and the differences in concentration of the marker compounds. The results show that a metabolomics-based approach is also suitable for differentiation of origin, even if the sample sites are close to each other.
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Abies , Picea , Cromatografía Liquida , Metabolómica , Espectrometría de Masas en Tándem , MaderaRESUMEN
Accurate identification of species from timber is an essential step to help control illegal logging and forest loss. However, current approaches to timber identification based on morphological and anatomical characteristics have limited species resolution. DNA barcoding is a proven tool for plant species identification, but there is a need to build reliable reference data across broad taxonomic and spatial scales. Here, we construct a species barcoding library consisting of 1550 taxonomically diverse timber species from 656 genera and 124 families, representing a comprehensive genetic reference data set for Chinese timber species and international commercial traded timber species, using four barcodes (rbcL, matK, trnH-psbA, and ITS2). The ITS2 fragment was found to be the most efficient locus for Chinese timber species identification among the four barcodes tested, both at the species and genus level, despite its low recovery rate. Nevertheless, the barcode combination matK+trnH-psbA+ITS2 was required as a complementary barcode to distinguish closely related species in complex data sets involving internationally traded timber species. Comparative analyses of family-level discrimination and species/genus ratios indicated that the inclusion of closely related species is an important factor affecting the resolution ability of barcodes for timber species verification. Our study indicates that although nuclear ITS2 is the most efficient single barcode for timber species authentication in China, complementary combinations like matK+trnH-psbA+ITS2 are required to provide broader discrimination power. These newly-generated sequences enrich the existing publicly available databases, especially for tropical and subtropical evergreen timber trees and this current timber species barcode reference library can serve as an important genetic resource for forestry monitoring, illegal logging prosecution and biodiversity projects.
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Código de Barras del ADN Taxonómico , Árboles , China , ADN de Plantas/genética , Bosques , Humanos , Análisis de Secuencia de ADN , Especificidad de la Especie , Árboles/genéticaRESUMEN
The determination of the geographical origin of wood can be highly relevant for several reasons: On the one hand, it can help to prevent illegal logging and timber trade, on the other hand, it is of special interest for archaeological artefacts made of wood, as well as for a variety of biological questions. For this reason, different extraction methods were first tested for the analysis of polar and non-polar metabolites using liquid chromatography coupled electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS). A two-phase extraction with chloroform, methanol and water proved to be particularly successful. Subsequently, cedrela (Cedrela odorata) samples from South America were measured to distinguish geographic origin. Using multivariate data analysis, numerous origin-dependent differences could be extracted. The identification of the marker substances indicated that several metabolic pathways were affected by the geographical influences, some of them probably indicating pest infections.
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Cedrela/metabolismo , Geografía , Metaboloma , Metabolómica/métodos , Espectrometría de Masa por Ionización de Electrospray/métodos , Madera/metabolismo , Acetatos/análisis , Cromatografía Líquida de Alta Presión/métodos , Ciclopentanos/análisis , Redes y Vías Metabólicas , Metanol/análisis , Oxilipinas/análisis , Análisis de Componente PrincipalRESUMEN
Illegal logging is a major threat to forests in Peru, in the Amazon more broadly, and in the tropics globally. In Peru alone, more than two thirds of logging concessions showed unauthorized tree harvesting in natural protected areas and indigenous territories, and in 2016 more than half of exported lumber was of illegal origin. To help combat illegal logging and support legal timber trade in Peru we trained a convolutional neural network using transfer learning on images obtained from specimens in six xylaria using the open source, field-deployable XyloTron platform, for the classification of 228 Peruvian species into 24 anatomically informed and contextually relevant classes. The trained models achieved accuracies of 97% for five-fold cross validation, and 86.5 and 92.4% for top-1 and top-2 classification, respectively, on unique independent specimens from a xylarium that did not contribute training data. These results are the first multi-site, multi-user, multi-system-instantiation study for a national scale, computer vision wood identification system evaluated on independent scientific wood specimens. We demonstrate system readiness for evaluation in real-world field screening scenarios using this accurate, affordable, and scalable technology for monitoring, incentivizing, and monetizing legal and sustainable wood value chains.
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Availability of and access to wood identification expertise or technology is a critical component for the design and implementation of practical, enforceable strategies for effective promotion, monitoring and incentivisation of sustainable practices and conservation efforts in the forest products value chain. To address this need in the context of the multi-billion-dollar North American wood products industry 22-class, image-based, deep learning models for the macroscopic identification of North American diffuse porous hardwoods were trained for deployment on the open-source, field-deployable XyloTron platform using transverse surface images of specimens from three different xylaria and evaluated on specimens from a fourth xylarium that did not contribute training data. Analysis of the model performance, in the context of the anatomy of the woods considered, demonstrates immediate readiness of the technology developed herein for field testing in a human-in-the-loop monitoring scenario. Also proposed are strategies for training, evaluating, and advancing the state-of-the-art for developing an expansive, continental scale model for all the North American hardwoods.
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The international trade in illegally logged and environmentally endangered timber has spurred enforcement agencies to seek additional technical procedures for the identification of wood species. All Dalbergia species are listed under the Convention on International Trade in Endangered Species (CITES) which is the reason this genus was chosen for study. Multiple sources of the heartwood from different Dalbergia species were extracted and chromatographic profiles collected by gas chromatography with high resolution quadrupole Time of Flight mass spectrometry (GC/QToF). The collected data was mined to select peaks and mass ions representative of the investigated Dalbergia species, and used to develop a Microsoft Excel® template offering immediate graphical representation of the results. Using wood specimens sourced from different xylaria, this graphical fingerprint proved adept at definitive identification of Dalbergia species. The CITES Appendix I species, D. nigra, was easily distinguished from D. melanoxylon and look-alike species of other genera. Similarly, a number of other Dalbergia species were differentiated using this current approach. Kernel discrimination analysis (KDA) was applied to increase the confidence of the species identification. The mislabeling of specimens appears to be common, and the emerging technique of GC/QToF in combination with other techniques, offers improved confidence in identification. GC/QToF further provides automation, the dimension of chromatography to avoid interferences, and production of reproducible electron impact positive (EI+) spectra. The prospect of building an EI+ spectral database for future wood identification is an important feature considering the limited accessibility of authenticated wood species specimens.
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Botánica/métodos , Comercio/ética , Comercio/métodos , Dalbergia/química , Dalbergia/clasificación , Cromatografía de Gases y Espectrometría de Masas , Madera/química , Análisis Discriminante , Especies en Peligro de Extinción , Internacionalidad , Iones/análisisRESUMEN
Eucalyptus is grown world-wide for paper pulp, solid wood, and other industries. Theft or illegal cutting of the trees causes hardship to owners of plantations and countries whose economies rely on the sale and export of eucalyptus products. Unfortunately, many of these crimes go unpunished due to lack of forensic evidence. Over 1200 short tandem repeat (STR) markers have been identified in the genomes of genus Eucalyptus and related species. However, their importance and utility in aiding forensic investigations of wood theft have not been explored. This study evaluated nine STRs for diversity and applied them to a case involving suspected wood theft. As expected, three dinucleotide STR markers showed greater variability but resulted in harder to interpret profiles. Four STR tetranucleotide markers evaluated in this study were found to contain additional repeat structures (dinucleotide or trinucleotide) that enhanced their variability but resulted in profiles with peaks at multiple stutter positions and heterozygote peak imbalance. The most promising STR markers were EGM37 and EMBRA 1374. Though less variable, they yielded robust and reproducible DNA profiles. All nine STR markers were applied to a case involving suspected wood theft. Samples were collected from seized wood and from remaining stumps in a plantation. No DNA match was found, thus eliminating the evidence samples as having originated from the forest. Dendrochronology analysis also resulted in an exclusion. This case study represents the first report using STR markers in any eucalyptus species to provide DNA evidence in a case of suspected wood theft.