The intestinal microbiota of a Risso's dolphin (Grampus griseus): possible relationships with starvation raised by macro-plastic ingestion.

Ingesting marine plastics is increasingly common in cetaceans, but little is known about their potential effects. Here, by utilizing 16S rRNA gene sequencing, we profiled the intestinal bacterial communities of a stranded Risso's dolphin (Grampus griseus) which died because of the ingestion of rubber gloves. In this study, we explored the potential relationships between starvation raised by plastic ingestion with the dolphin gut microbiota. Our results showed significant differences in bacterial diversity and composition among the different anatomical areas along the intestinal tract, which may be related to the intestinal emptying process under starvation. In addition, the intestinal bacterial composition of the Risso's dolphin showed both similarity and divergence to that of other toothed whales, suggesting potential roles of both host phylogeny and habitat shaping of the cetacean intestinal microbiome. Perhaps, the microbiota is reflecting a potentially disordered intestinal microbial profile caused by the ingestion of macro-plastics which led to starvation. Moreover, two operational taxonomic units (0.17% of the total reads) affiliated with Actinobacillus and Acinetobacter lwoffii were detected along the intestinal tract. These bacterial species may cause infections in immunocompromised dolphins which are malnourished. This preliminary study profiles the intestinal microbiota of a Risso's dolphin, and provides an additional understanding of the potential relationships between starvation raised by ingesting macro-plastics with cetacean gut microbiota.

Occurrence and distribution of meso- and macroplastics in the water, sediment, and fauna of the Nile River, Egypt.

The present study described the most recent findings concerning the abundance and distribution of plastic in water, sediment, and fauna in the Nile River of Upper Egypt as an interesting research point. The findings revealed that plastics were abundant in the water, sediments, fish, and crayfish throughout the sites. The Nagaa Hammadi site has the highest abundance of meso- and macroplastics in its water and sediment. African catfish had the highest abundance of meso- and macroplastics compared to the other species, while Nile tilapia had no meso- or macroplastics in its alimentary canal or gills in all sites. The Edfu site has the highest abundance of mesoplastics in the alimentary canals of African catfish, while the Nagaa Hammadi site has the highest abundance of mesoplastics in the gills, and macroplastics appeared only in the alimentary canal of African catfish from the El-wasta site. Only mesoplastics were found in the crayfish's alimentary canal, with the Nagaa Hammadi site having the highest abundance. No macroplastics were detected in the crayfish's gills or alimentary canal. Additionally, this work lets us understand how plastics behave in freshwater environments, and it is a step toward decision-makers taking appropriate measures to reduce their risk.

Maximizing Realism: Mapping Plastic Particles at the Ocean Surface Using Mixtures of Normal Distributions.

Current methods of characterizing plastic debris use arbitrary, predetermined categorizations and assume that the properties of particles are independent. Here we introduce Gaussian mixture models (GMM), a technique suitable for describing non-normal multivariate distributions, as a method to identify mutually exclusive subsets of floating macroplastic and microplastic particles (latent class analysis) based on statistically defensible categories. Length, width, height and polymer type of 6,942 particles and items from the Atlantic Ocean were measured using infrared spectroscopy and image analysis. GMM revealed six underlying normal distributions based on length and width; two within each of the lines, films, and fragments categories. These classes differed significantly in polymer types. The results further showed that smaller films and fragments had a higher correlation between length and width, indicating that they were about the same size in two dimensions. In contrast, larger films and fragments showed low correlations of height with length and width. This demonstrates that larger particles show greater variability in shape and thus plastic fragmentation is associated with particle rounding. These results offer important opportunities for refinement of risk assessment and for modeling the fragmentation and distribution of plastic in the ocean. They further illustrate that GMM is a useful method to map ocean plastics, with advantages over approaches that use arbitrary categorizations and assume size independence or normal distributions.

Rapid Characterization of Macroplastic Input and Leakage in the Ganges River Basin.

Efforts to understand macroplastic pollution have primarily focused on coastal and marine environments to the exclusion of freshwater, terrestrial, and urban ecosystems. To better understand macroplastics in the environment and their sources, a dual approach examining plastic input and leakage can be used. In this study, litter aggregation pathways at 40 survey sites with varying ambient population counts in the Ganges River Basin were surveyed in pre- and postmonsoon seasons. We examine active litter leakage using transect surveys of on-the-ground items, in conjunction with assessments of single-use plastic consumer products at the point of sale. We find that sites with low populations have a significantly higher number of littered items per 1,000 people than those with mid to high populations. Over 75% of litter items were plastics or multimaterial items containing plastic, and tobacco products and plastic food wrappers were the most recorded items. There was no significant variation of litter densities pre- and postmonsoon. Most single-use plastic consumer products were manufactured in-country, but approximately 40% of brands were owned by international companies. Stratified sampling of active litter input and consumer products provides a rapid, replicable snapshot of plastic use and leakage.

Overview of global status of plastic presence in marine vertebrates.

The presence of plastic in the environment is generating impacts on all habitats and has become a major global problem in marine megafauna. Macroplastics can cause entanglement, ingestion and loss of suitable habitats. In addition to entanglement problems, there is evidence that plastics are entering the food web through ingestion by marine organisms, which could ultimately be affecting humans. Much of the available information on the impact of plastic in biota is scattered and disconnected due to the use of different methodologies. Here, we review the variety of approaches and protocols followed to assess macro- and microplastic ingestion in marine vertebrates such as sea turtles, cetaceans and fishes in order to offer a global overview of their current status. The analysis of 112 studies indicates the highest plastic ingestion in organisms collected in the Mediterranean and Northeast Indian Ocean with significant differences among plastic types ingested by different groups of animals, including differences in colour and the type of prevalent polymers. In sea turtles, the most prevalent types of plastics are white plastics (66.60%), fibres (54.54%) and LDPE polymer (39.09%); in cetaceans, white macro- and microplastics (38.31%), fibres (79.95%) and PA polymer (49.60%); and in fishes, transparent plastics (45.97%), fibres (66.71%) and polyester polymer (36.20%). Overall, clear fibre microplastics are likely the most predominant types ingested by marine megafauna around the globe.

Increased plastic pollution due to COVID-19 pandemic: Challenges and recommendations.

Plastics have become a severe transboundary threat to natural ecosystems and human health, with studies predicting a twofold increase in the number of plastic debris (including micro and nano-sized plastics) by 2030. However, such predictions will likely be aggravated by the excessive use and consumption of single-use plastics (including personal protective equipment such as masks and gloves) due to COVID-19 pandemic. This review aimed to provide a comprehensive overview on the effects of COVID-19 on macroplastic pollution and its potential implications on the environment and human health considering short- and long-term scenarios; addressing the main challenges and discussing potential strategies to overcome them. It emphasises that future measures, involved in an emergent health crisis or not, should reflect a balance between public health and environmental safety as they are both undoubtedly connected. Although the use and consumption of plastics significantly improved our quality of life, it is crucial to shift towards sustainable alternatives, such as bio-based plastics. Plastics should remain in the top of the political agenda in Europe and across the world, not only to minimise plastic leakage and pollution, but to promote sustainable growth and to stimulate both green and blue- economies. Discussions on this topic, particularly considering the excessive use of plastic, should start soon with the involvement of the scientific community, plastic producers and politicians in order to be prepared for the near future.

The artificialization in the sediment profiles of the streams in the Água Branca basin - Itirapina, São Paulo, Brazil.

River sediments have the capability to accumulate and absorb traces of anthropic environmental degradation. In this work, we investigated the effects of urban land use on the composition of physical sediment. For this purpose, sediment profiles were collected in the wet and dry seasons and in locations with mixed land-use. For the sediment analysis, particle size separation, organic matter degradation, visual analysis of the thickest fraction using a magnifying glass and stereomicroscope analysis of the particle size material corresponding to the sands were performed. It was observed that the sedimentation of synthetic materials and plastics (fragments and fibres) were the most frequently found materials. More intense urban occupation areas and with less riparian vegetation suffer more from this degradation than rural areas or areas with preserved riparian vegetation. The dam in the basin accumulates more materials (macro and microplastics) and has the role of containing and preventing these residues from being transported downstream. The importance of investing in environmental management measures is emphasized, and based on the sampled sites assessed, the restoration of riparian vegetation, greater inspection of inadequate waste disposal and public cleaning actions are suggested, including actions in the Tibiriçá dam.

Anthropogenic litter in freshwater environments - Study on lake beaches evaluating marine guidelines and aerial imaging.

Studies on macroplastic pollution in freshwater systems are rare compared to the marine environment. Nevertheless, freshwater systems are worthy to be equally investigated as they are pathways of plastic to the ocean and lakes may act as (temporary) sinks. The aim of this study was to identify sources for plastics and influences on its distribution in a limnic environment. Anthropogenic litter (>5 mm) was monitored semi-annually over a three-year period at four sandy bank border segments of Lake Tollense in Mecklenburg-Western Pomerania, Germany. The selected beaches represent different expositions and vary in their level of anthropogenic activity. Considering all six samplings, mean abundance of anthropogenic litter is 0.2 ± 0.1 items/m2 or 130.9 ± 91.0 items/100 m beach length. The averaged mass of anthropogenic litter is 0.5 ± 1.0 g/m2 or rather 218.7 ± 284.6 g/100 m. Plastic consistently is the predominate material (72%) and cigarette butts are the most found items. A higher pollution by anthropogenic litter is found at the end of tourist season unveiling the impact of anthropogenic activity on litter abundance. Additionally, litter transport via tributaries into the lake plays a role. Testing the detection of anthropogenic litter via aerial images taken by unmanned aerial vehicles resulted in good recovery rates when minimizing the flight height. Furthermore, the analysis of anthropogenic litter distribution displayed on the images showed litter accumulation areas at the border of sandy beach areas. The deployment of marine guidelines in a freshwater environment did work well, however, small changes in the protocol are suggested for future lake beach studies dealing with anthropogenic litter pollution.

A catchment-scale perspective of plastic pollution.

Plastic pollution is distributed across the globe, but compared with marine environments, there is only rudimentary understanding of the distribution and effects of plastics in other ecosystems. Here, we review the transport and effects of plastics across terrestrial, freshwater and marine environments. We focus on hydrological catchments as well-defined landscape units that provide an integrating scale at which plastic pollution can be investigated and managed. Diverse processes are responsible for the observed ubiquity of plastic pollution, but sources, fluxes and sinks in river catchments are poorly quantified. Early indications are that rivers are hotspots of plastic pollution, supporting some of the highest recorded concentrations. River systems are also likely pivotal conduits for plastic transport among the terrestrial, floodplain, riparian, benthic and transitional ecosystems with which they connect. Although ecological effects of micro- and nanoplastics might arise through a variety of physical and chemical mechanisms, consensus and understanding of their nature, severity and scale are restricted. Furthermore, while individual-level effects are often graphically represented in public media, knowledge of the extent and severity of the impacts of plastic at population, community and ecosystem levels is limited. Given the potential social, ecological and economic consequences, we call for more comprehensive investigations of plastic pollution in ecosystems to guide effective management action and risk assessment. This is reliant on (a) expanding research to quantify sources, sinks, fluxes and fates of plastics in catchments and transitional waters both independently as a major transport routes to marine ecosystems, (b) improving environmentally relevant dose-response relationships for different organisms and effect pathways, (c) scaling up from studies on individual organisms to populations and ecosystems, where individual effects are shown to cause harm and; (d) improving biomonitoring through developing ecologically relevant metrics based on contemporary plastic research.

Macrolitter budget and spatial distribution in a groyne field along the Waal river.

Current research on riverine macrolitter does not yet provide a theoretic framework on the dynamics behind its accumulation and distribution along riverbanks. In an attempt to better understand these dynamics a detailed field survey of three months was conducted in which location of macrolitter items within a single groyne field along the Waal riverbanks was tracked. The data provided insight into the daily changing patterns of spatial item distribution with respect to the waterline. Furthermore, the rates of item uptake and deposition were monitored and related to hydrologic fluctuations. Uptake was initiated by rising water levels and was generally higher when the water level increased faster. Deposition occurred continuously, despite hydrologic fluctuations. This caused the riverbank macrolitter budget to be positive during stable or dropping water levels and negative during rising water levels. Although the results show clear patterns an extended monitoring duration is required to fully understand the fate of plastic objects.

Catchment scale assessment of macroplastic pollution in the Odaw river, Ghana.

Catchment-scale plastic pollution assessments provide insights in its sources, sinks, and pathways. We present an approach to quantify macroplastic transport and density across the Odaw catchment, Ghana. We divided the catchment into the non-urban riverine, urban riverine, and urban tidal zones. Macroplastic transport and density on riverbanks and land were monitored at ten locations in December 2021. The urban riverine zone had the highest transport, and the urban tidal zone had the highest riverbank and land macroplastic density. Water sachets, soft fragments, and foam fragments were the most abundant items. Our approach aims to be transferable to other catchments globally.

Microplastic concentration, characterization, and size distribution in the Delaware Bay estuary.

Microplastic (MP) pollution has been widely reported across water matrices including in estuaries, which are important for the understanding of oceanic MPs. Estuaries can greatly alter the fate, transport, size distribution, and abundance of plastic pollution. The aim of this study was to quantify and characterize MP pollution in the Delaware Bay estuary USA, including the size distribution. Samples (N = 31) were collected from the mouth of the Delaware River to the coastal ocean including multiple frontal zones across two sampling campaigns (2019 and 2022). MP were extracted from the collected particles using wet peroxide oxidation and density separation with saturated sodium chloride. Particles collected on 500 µm mesh sieves were analyzed via Fourier transform infrared (FTIR) spectroscopy. Across all samples, 324 of the 1015 particles analyzed were MP, and 11 macroplastics were observed. MP concentrations ranged from below detection to 4.12 MP/m3 (mean 0.34 ± 0.80 MP/m3). No significant differences were observed between sampling sites; nonetheless, the two highest MP concentrations were observed when sampling along frontal zones with visible debris including macroplastics. Polyethylene (53%) and polypropylene (43%) were the most abundant polymers observed. The majority of the non-plastic particles were classified as particulate natural organic matter (82% of non-plastics). Particles from samples collected during 2022 (N = 864) also had color, morphology, and two size dimensions recorded. MP particle size was significantly associated with sampling site, with the coastal ocean sampling site generally having the smallest MPs. A correlation between total post-extraction particles and total plastic particles was observed. Aspect ratios for the plastics ranged from one to 40.7, with larger ratios for fibers, with a mean (±standard deviation) of 3.39 ± 4.72 (unitless). These aspect ratios can be used to select shape factors used to estimate the total volume of MP in the studied size range. Overall, these results can help inform fate, transport, and risk assessments related to estuarine plastic pollution.

Differentiation of petro-sourced plastic microfilaments from organic microfilaments by SEM-EDX in environmental samples.

Quantifying microplastics (MPs) in marine environments is challenging due to the complexities of differentiation from other materials. This study aims to distinguish petro-sourced plastic microfilaments from organic ones in environmental samples using scanning electron microscope coupled with energy dispersive X-ray (SEM-EDX) analysis. Therefore, 38 particles resembling MPs (PRMPs) from sediments and organisms in Madagascar were analyzed. MPs identification thresholds were determined from SEM-EDX analysis of macroplastics and seaweed, the primary sources of microfilaments. SEM-EDX results of the 38 PRMPs were compared with identification/misidentification made by five MPs observation experts. Results indicated that a carbon/oxygen (C/O) ratio >1 distinguished macroplastics and MPs from other sources (seaweed and organic microfilaments). SEM-EDX analysis identified 81.58% of PRMPs as MPs. The average error made by one observer was 36.32%. These findings highlight the need for a method to assess identification error in MPs quantification and recommend SEM-EDX analysis of microfilaments to gauge observer error.

Macro and microplastic pollution in Romania: addressing knowledge gaps and potential solutions under the circular economy framework.

Background: This review reveals the role of linear economy prevalence and mismanagement practices in plastic pollution of aquatic and terrestrial environments and related knowledge gaps in Romania while outlining downstream and upstream solutions to reduce plastic pollution and adopt circular economy strategies. Thus, the major aim of this study is the investigation of the stage of scientific knowledge concerning all these demands in the Romanian context. Methodology: This work integrates two main approaches: (i) a bibliometric analysis fed by Web of Science and Scopus databases to reveal the current coverage of peer-reviewed literature related to plastic waste in Romania and (ii) a subject-based review to underline the main themes related to plastic waste management, plastic pollution, and mitigating options in Romania in line with circular economy principles. Results: Reducing plastic pollution requires scientific knowledge, multi-sectoral cooperation, and societal awareness. Following this, the topics of plastic waste and plastic pollution appeared to be under-investigated in the literature considering Romania as a case study and concentrated around the 2020 year, emphasizing, in this way, the trendiness of plastic waste concerns and their management in the current research landscape. Our analysis points out that: (i) Romania is facing massive plastic pollution requiring solid improvements in waste management performances; (ii) few peer-reviewed research studies are performed in Romania for both macro and microplastic concerns with unknown pollution levels in most of its geographical regions; (iii) the plastic waste management is still understudied here, while waste statistics are poorly available at local levels; (iv) the perspectives of circular economy transition are still limited, feeding the plastic pollution in the coming years. Conclusions: Several knowledge gaps are identified and must be covered by future research such as (i) adjusting mismanaged plastic waste levels to regional waste management performances and determining littering rates in urban and rural areas to improve the plastic pollution modeling inputs; (ii) examining plastic pollution associated with landfill sites and waste imports; (iii) assessing the sectoral contributions to macro and microplastic pollution of aquatic environments related to municipalities, tourist destinations, agriculture, etc.; (iv) determining retention levels of plastic in river basins and role of riparian vegetation; (v) analyzing microplastics presence in all types of freshwater environments and interlinkage between macroplastic fragmentation and microplastic; (vi) assessing the plastic loads of transboundary rivers related to mismanagement practices; (vii) determining concentrations of microplastics in air, soil, and other land use ecosystems.

On the vertical structure of non-buoyant plastics in turbulent transport.

Plastic pollution is overflowing in rivers. A limited understanding of the physics of plastic transport in rivers hinders monitoring, the prediction of plastic fate and restricts the implementation of effective mitigation strategies. This study investigates two unexplored aspects of plastic transport dynamics across the near-surface, suspended and bed load layers: (i) the complex settling behaviour of plastics and (ii) their influence on plastic transport in river-like flows. Through hundreds of settling tests and thousands of 3D reconstructed plastic transport experiments, our findings show that plastics exhibit unique settling patterns and orientations, due to their geometric anisotropy, revealing a multimodal distribution of settling velocities. In the transport experiments, particle-bed interactions enhanced mixing beyond what established turbulent transport theories (Rouse profile) could predict in low-turbulence conditions, which extends the bed load layer beyond the classic definition of the bed load layer thickness for natural sediments. We propose a new vertical structure of turbulent transport equation that considers the stochastic nature of heterogeneous negatively buoyant plastics and their singularities.

Disentangling marine plastic impacts in Life Cycle Assessment: Spatially explicit Characterization Factors for ecosystem quality.

Inputs of persistent plastic items to marine environments continue to pose a serious and long-term threat to marine fauna and ecosystem health, justifying further interventions on local and global scales. While Life Cycle Assessment (LCA) is frequently used for sustainability evaluations by industries and policymakers, plastic leakage to the environment and its subsequent impacts remains absent from the framework. Incorporating plastic pollution in the assessments requires development of both inventories and impact assessment methods. Here, we propose spatially explicit Characterization Factors (CF) for quantifying the impacts of plastic entanglement on marine megafauna (mammals, birds and reptiles) on a global scale. We utilize Lagrangian particle tracking and a Species Sensitivity Distribution (SSD) model along with species susceptibility records to estimate potential entanglement impacts stemming from lost plastic-based fishing gear. By simulating plastic losses from fishing hotspots within all Exclusive Economic Zones (EEZs) we provide country-specific impact estimates for use in LCA. The impacts were found to be similar across regions, although the median CF associated with Oceania was higher compared to Europe, Africa and Asia. Our findings underscore the presence of susceptible species across the world and the transboundary issue of plastic pollution. We discuss the application of the factors and identify areas of further refinement that can contribute towards a comprehensive assessment of macroplastic pollution in sustainability assessments. Degradation and beaching rates for different types of fishing gear remain a research gap, along with population-level effects on marine taxa beyond surface breathing megafauna. Increasing the coverage of impacts specific to the marine realm in LCA alongside other stressors can facilitate informed decision-making towards more sustainable marine resource management.

Macroplastic litter colonization by stream macroinvertebrates relative to that of plant litter: A meta-analysis.

Environmental pollution by anthropogenic litter is a global concern, but studies specifically addressing the interaction between macroplastics and macroinvertebrates in streams are scarce. However, several studies on plant litter decomposition in streams have also used plastic strips as a methodological approach to assess if macroinvertebrates colonize plant litter mostly as a substrate or a food resource. Looking at these studies from the plastic strips perspective may provide useful information on the interaction between macroplastics and macroinvertebrates in streams. I carried out a meta-analysis of 18 studies that have compared macroinvertebrate colonization of macroplastic litter and plant litter in streams to estimate the overall macroinvertebrate colonization of macroplastic litter relative to plant litter, and identify moderators of this difference. Macroinvertebrate colonization of macroplastic litter was overall lower (by âˆ¼ 40%) compared with plant litter. However, differences in macroinvertebrate colonization between macroplastic litter and plant litter were observed when considering leaf litter but not wood litter, which may be a poorer substrate and food resource for macroinvertebrates. Also, differences in macroinvertebrate colonization between macroplastic litter and leaf litter were observed for shredders, collectors and predators, but not for grazers that may feed on the biofilm developed on macroplastics. Macroplastic litter supported lower macroinvertebrate density, biomass, abundance, and richness, but higher macroinvertebrate diversity than leaf litter. Higher macroinvertebrate diversity on macroplastic litter may have occurred when macroplastics represented more heterogeneous substrates (e.g., mixture of plastic types) than leaf litter (e.g., needles). Differences in macroinvertebrate abundance between macroplastic litter and leaf litter were not significantly affected by plastic type, mesh opening size, plant functional group or plant identity. By testing previously untested hypotheses, this meta-analysis guides future empirical studies. Future studies should also consider the geographical areas most affected by macroplastic pollution and the plastic types most often found in the streams.

Land-based plastic leakage into the aquatic environment from municipal solid waste - Waste flow diagram applied to Tuy Hoa City, Phu Yen, Vietnam.

Plastic mismanagement and its subsequent pollution by rapid economic development and urbanisation pose significant challenges for modern world society. Notwithstanding one of the main sources for macro plastic leakage into the ocean from land, precise assessment of plastic pollution origins from Southeast Asia is yet to be clearly examined. In order to make informed decisions and prioritise areas of improvement it is required to better understand the waste leakage dynamics at the local level. In this work, the Waste Flow Diagram (WFD) was applied to understand the sources and fates of plastics leaking from the solid waste management system for the case of Tuy Hoa City, Phu Yen, Vietnam. The study shows scenarios of leakage into the aquatic environment ranging from 0.8 to 2.7 kg/cap/year, which originates mainly from the collection system. Targeted improvements to this stage of the service could reduce leakages and the overall environmental impacts of mismanaged plastic waste. The results of this study show the necessity and importance of having up to date and reliable data to better inform stakeholders and service planning, facilitating efficient action against plastic pollution. As the first peer-reviewed scientific article critically applying the WFD, this work highlights the steps and challenges of the methodology and critically analyses different methodological pathways.

A particle tracking model approach to determine the dispersal of riverine plastic debris released into the Indian Ocean.

Although the Indian Ocean receives a large amount of land-based plastic waste, the studies on pathways of riverine plastic debris are limited to date. Therefore, a particle tracking model that included ocean surface currents, horizontal diffusion, Stokes drift, windage, and beaching/re-drifting processes was developed to reproduce the behavior of riverine plastic debris in the Indian Ocean. The modeled particles were released in the model domain based on riverine plastic debris database. The maximum abundance of beached particles occurred during the southwesterly monsoon season, particularly in the Bay of Bengal. The particles released from the rivers were trapped in the northern Indian Ocean unless both Stokes drift and windage were excluded from transportation velocity. These results suggest that the riverine plastic debris was trapped in the northern Indian Ocean until it fragmented into less buoyant small microplastics drifting in the subsurface layer, free from windage and Stokes drift at increasing depths.

Plastic pollution transcends marine protected area boundaries in the eastern tropical and south-eastern Pacific.

The Eastern Tropical and South-Eastern Pacific region is of global biodiversity importance. At COP26, the governments of Costa Rica, Panama, Colombia, and Ecuador committed to the expansion of existing MPAs to create a new Mega MPA, safeguarding the Eastern Tropical Pacific Marine Corridor. It offers a profound step forward in conservation efforts but is not specifically designed to protect against the more diffuse anthropogenic threats, such as plastic pollution. We combine published data with our own unpublished records to assess the abundance and distribution of plastic pollution in the region. Macro- and microplastic concentrations varied markedly and were not significantly different when comparing areas inside and outside existing MPA boundaries. These findings highlight the diffuse and complex nature of plastic pollution and its ubiquitous presence across MPA boundaries. Understanding the sources and drivers of plastic pollution in the region is key to developing effective solutions.