Film dressings from Thai mango seed kernel extracts versus nanocrystalline silver dressings in antibacterial properties.

Introduction: The extract from the Mango Seed Kernel (MSK) has been documented to exhibit antibacterial activity against Gram-positive and Gram-negative bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa. This suggests that biomaterials containing MSK extract could be a viable alternative to conventional wound treatments, such as nanocrystalline silver dressings. Despite this potential, there is a notable gap in the literature regarding comparing the antibacterial effectiveness of MSK film dressings with nanocrystalline silver dressings. This study aimed to develop film dressings containing MSK extract and evaluate their antibacterial properties compared to nanocrystalline silver dressings. Additionally, the study aimed to assess other vital physical properties of these dressings critical for effective wound care. Materials and methods: We prepared MSK film dressings from two cultivars of mango from Thailand, 'Chokanan' and 'Namdokmai'. The inhibition-zone method was employed to determine the antibacterial property. The morphology and chemical characterization of the prepared MSK film dressings were examined with scanning electron microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR), respectively. The absorption of pseudo-wound exudate and water vapor transmission rate (WVTR) of film dressings were evaluated. Results: The results showed that 40% of MSKC film dressing had the highest inhibition zone (20.00 ± 0.00 mm against S. aureus and 17.00 ± 1.00 mm against P. aeruginosa) and 20%, 30%, and 40% of MSKC and MSKN film dressings had inhibition zones similar to nanocrystalline silver dressing for both S. aureus and P. aeruginosa (p > 0.05). In addition, all concentrations of the MSK film dressings had low absorption capacity, and Chokanan MSK (MSKC) film dressings had a higher WVTR than Namdokmai MSK (MSKN) film dressings. Conclusion: 20%, 30%, and 40% of MSK film dressing is nearly as effective as nanocrystalline silver dressing. Therefore, it has the potential to be an alternative antibacterial dressing and is suitable for wounds with low exudate levels.

Supercritical Fluid Extraction of Phenolic Compounds from Mango (Mangifera indica L.) Seed Kernels and Their Application as an Antioxidant in an Edible Oil.

Phenolic compounds from mango (M. indica) seed kernels (MSK) var. Sugar were obtained using supercritical CO2 and EtOH as an extraction solvent. For this purpose, a central composite design was carried out to evaluate the effect of extraction pressure (11-21 MPa), temperature (40-60 °C), and co-solvent contribution (5-15% w/w EtOH) on (i) extraction yield, (ii) oxidative stability (OS) of sunflower edible oil (SEO) with added extract using the Rancimat method, (iii) total phenolics content, (iv) total flavonoids content, and (v) DPPH radical assay. The most influential variable of the supercritical fluid extraction (SFE) process was the concentration of the co-solvent. The best OS of SEO was reached with the extract obtained at 21.0 MPa, 60 °C and 15% EtOH. Under these conditions, the extract increased the OS of SEO by up to 6.1 ± 0.2 h (OS of SEO without antioxidant, Control, was 3.5 h). The composition of the extract influenced the oxidative stability of the sunflower edible oil. By SFE it was possible to obtain extracts from mango seed kernels (MSK) var. Sugar that transfer OS to the SEO. These promissory extracts could be applied to foods and other products.

Extraction of bioactive compounds from mango (Mangifera indica L. var. Carabao) seed kernel with ethanol-water binary solvent systems.

Mango seed kernel, a by-product of the processing industry, can be valorized as a potential source of bioactive compounds. Binary mixtures of ethanol and water, used in solid-liquid extraction (SLE), have drawn interest as an effective means of recovering phytochemicals from plant materials because these solvents can be used in food applications and their synergistic effect makes them a superior solvent over their pure counterparts. Total phenolic content (TPC) and HPLC chromatograms of each ethanolic extract revealed that ethanol concentration had a significant effect on phenolic compound recovery, wherein, TPC of mango kernel varied from 18.19 to 101.68 mg gallic acid equivalence (GAE) per gram of sample. Subsequently, the antioxidant activities (AOAc) of the extracts, measured by scavenging activities with the DPPH+ (1,1-diphenyl-2-picrylhydrazyl) radical and ferric reducing antioxidant power (FRAP) assay, ranged from 8.19 to 85.45 mmol/L and 3.82-55.61 mmol/L Trolox equivalence, respectively. The solvent containing 50% (w/w) ethanol-water had the highest TPC and exhibited the most potent reducing and radical scavenging activities. With the use of an HPLC-UV/Vis, gallic acid, caffeic acid, rutin and penta-O-galloyl-ß-d-glucose were identified to be present in the mango seed kernel. Results show that the mango seed kernel is a viable source of bioactive compounds which can be recovered with water-ethanol binary solvent systems.

Characterization and storage stability of the extract of Thai mango (Mangifera indica Linn. Cultivar Chok-Anan) seed kernels.

Qualitative analysis of hydrolysable extract from mango (Mangifera indica Linn. cultivar Chok-Anan) seed kernel was performed by means of reversed phase high-performance liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry (RPHPLC-DAD-ESI-MS). The main phenolic compound was identified as methyl gallate by comparing their retention time, UV-vis absorption spectra and mass spectra with a reference standard. Quantification of phenolic compounds was performed by HPLC-DAD, which revealed that the extract contained total phenolics at a concentration of 194.1 mg GAE/g dry weight of mango seed kernel (MSK), of which 85.7% was identified as methyl gallate. In addition, the antioxidant activities of the extract and the main compound were assessed by the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays, by the ferric thiocyanate method and by an assay of metal chelating activity. Tyrosinase inhibition was also investigated. Furthermore, the antioxidant capacity and the total phenolic content of MSK extract stored in a plastic (polyethylene) PE bag decreased during storage at freezing (-20 °C), refrigerated (7 °C) and room (28-32 °C) temperature for 182 days. The loss of antioxidant capacity and total phenolic content increased at higher storage temperatures for more than 182 days.

Optimization of an ultrasound-assisted extraction method to obtain gallic acid-rich extracts from mango seed kernels.

Gallic acid is a widely recognized bioactive compound that falls under the category of secondary polyphenolic metabolites and is fairly found in mango fruit waste, specifically in mango seed kernel (MSK). This study aimed to adopt a green extraction approach to extract this valuable compound via ultrasound-assisted extraction (UAE) without using organic solvents but only water to obtain hazard-free extracts, and the cost of extraction can be minimal. pH (2-8), solvent ratio (20-60 mL/g), temperature (30-60°C) and time (30-60 min) of extraction were the independent variables used for extraction optimization. Single-factor experiments to obtain working ranges for selected extraction variables were carried out. A central composite design using response surface methodology was used to determine the optimum condition to obtain the maximum yield of gallic acid from MSK. The optimized extraction conditions were 3.9 pH, 36.25 mL/g solvent ratio, and 39.4°C of extraction temperature for 21.3 min. As a result, the optimized yield was 5.76 ± 0.41 mg/g, which was comparably equal to and/or better than the other solvent extraction systems. The results showed that gallic acid could efficiently be extracted via UAE under these optimal conditions. It is safer than extraction systems involving hazardous solvents that can be feasibly used for its nutraceutical and therapeutic applications.

Flavonoid glycosides and ellagic acid cognates from defatted African mango (Irvingia gabonensis) seed kernel.

Seventeen compounds of diverse classes including four flavonoid glycosides, five ellagic acid derivatives, and eight other metabolites were isolated from the methanolic extract of the defatted seed kernel of Irvingia gabonensis. Among the isolates, quercetin 3-O-methyl-4'-[α-L-rhamnopyranosyl-(1→3)]-O-α-L-rhamnopyranoside (1) and 3,3'-di-O-methyl-4'-O-α-L-rhamnopyranosylellagic acid 4-sulfate ester (5) were found to be previously undescribed. Structure elucidation was mainly achieved by the interpretation of 1D and 2D NMR and HRESIMS spectral data. Though compound 6 was previously reported, its 13C NMR data is being reported herein for the first time. To the best of our literature search knowledge, this is the first phytochemical report on I. gabonensis seed kernels.

Recent development of eco-friendly nanocomposite carbon paste electrode for voltammetric determination of Cd(II) in real samples.

Using eco-friendly, cheap, and available adsorbents is promising for the determination of metal ions. So, this study focuses on the modification of graphite reinforcement carbon paste electrode (GRCPE) with mango seed kernel (MSK) for voltammetric determination of Cd(II). Moreover, to increase the surface area of this adsorbent, it was prepared in nanosized that formed nanoparticles of mango seed kernel (MSK-NPs). The developed nanocomposite electrode of carbon paste electrode modified with nanoparticles of mango seed kernel (MSK-NPs@GRCPE) was characterized using Fourier transform infrared (FTIR) and Scanning electron microscopy (SEM). The effect of pH, buffer solution, and supporting electrolyte as experimental conditions were optimized through differential pulse adsorptive anodic stripping voltammetric method (DPAdASV). Britton-Robinson buffer pH = 3.9 at Eacc = - 1400 mV, tacc = 30 s, pulse width = 10 ms and sampling time = 8 ms were the optimum conditions for determination of Cd(II). The LOD and LOQ of MSK-NPs@GRCPE were calculated at 5.44 × 10-9 and 1.65 × 10-8 M, respectively. Compared with bare graphite reinforcement carbon paste electrode (BGRCPE), the nanocomposite MSK-NPs@GRCPE has a lower detection limit, indicating that the presence of MSK-NPs could greatly improve the response to Cd(II). The practical applicability of the electrode was verified by the determination of Cd(II) in chocolate and white rice samples. The results show high selectivity and sensitivity for Cd(II) in real samples.

Profiling of phenolic compounds, antimicrobial, antioxidant, and hemolytic activity of mango seed kernel using different optimized extraction systems.

Mango seed kernels (MSKs) have been reported to show antioxidant, antibacterial, and anti-inflammatory properties. This study explores the influence of different optimized extraction systems on the extraction of MSK. The effects on gallic acid (GA) content, total phenolic content (TPC), total flavonoid content (TFC), antioxidant, antimicrobial, and hemolytic activity of MSK extracts from different extraction systems (65.45% ethanol-ultrasound assisted extraction [UAE], 62% ethanol-incubator shaker, 19.4% ethanol-UAE, and 100% water-UAE) were assessed. Based on the results, a nonsignificant difference in phenolic (p = 0.222), flavonoids (p = 0.058), antioxidant (p = 0.165), and antimicrobial activity (p = 0.193) against Staphylococcus aureus whereas a significant difference (p < 0.0001) in hemolytic, GA content, and antimicrobial activity against Clostridium perfringens was observed. Among different extraction systems, aqueous extraction showed significantly lower hemolytic (1.09%) and higher GA content (4.72 mg/g) and comparable results in all other experiments; yield (32.40%), TPC (58.79 mg/g), TFC (2.16 mg/g), and antioxidant (73.19%). Hence, it has been concluded that aqueous extraction system could be considered a sustainable extraction system for practical applications. PRACTICAL APPLICATION: Aqueous extraction system could be a sustainable option for extraction of mango seed kernel for practical applications as it is readily available, cheap, nonflammable, and nontoxic.

Effect of atmospheric pressure non-thermal pin to plate plasma on the functional, rheological, thermal, and morphological properties of mango seed kernel starch.

This study aimed to investigate the effect of atmospheric pressure non-thermal pin-to-plate plasma on the functional, rheological, thermal, and morphological properties of mango seed kernel starch. As cold plasma contains highly reactive species and free radicals, it is expected to cause noticeable modifications in the attributes of starch treated. The isolated mango seed kernel starch was subjected to the plasma treatment of input voltages 170 and 230 V for 15 and 30 min of exposure. Water adsorption, swelling, and solubility at lower temperatures. There has been a significant reduction (p < 0.05) in pH values of starch from 7.09 to 6.16 and also the desirable reduction in turbidity values by 42.60%. However, there has been no significant change in the oil and water binding behavior of the starch. The FTIR spectra of MSKS demonstrate the formation of amines which contributes to the better hydrophilic nature of the starch. The structural modification has been adequately confirmed by SEM images. The maximum voltage and time combination, lead to depolymerization of starch which is supported by NMR spectra thus affecting thermal and rheological properties. The application of cold plasma-modified MSKS in food would facilitate stable and smooth textural development.

Potential cocoa butter substitute derived from mango seed kernel.

The present finding provides a new cocoa butter substitute in confectionary, which is derived from mango by-product (mango seed kernel). The study involves physicochemical characterization and the use of GCMS, FTIR, SEM, TGA, and NMR to prove that mango seed kernel derived fat is a good substitute for cocoa butter. Its texture, organoleptic properties and rancidity were also investigated. Its properties were similar to cocoa butter, with respect to palmitic, oleic, and stearic acids, and it had the ability to substitute 80 per cent of dark chocolate preparation for chocolate substitute. This recently developed cocoa substitute has the potential to address the global problem of cocoa butter scarcity, which is being exacerbated by rising population and improving economies.

Bioconversion of mango (Mangifera indica) seed kernel starch into bioethanol using various fermentation techniques.

The potential of mango seed kernel starch (MKS) as a feedstock for bioethanol production was evaluated in this study. Starch extraction and hydrolysis from mango kernel were studied. Fermentation methods included separate hydrolysis and fermentation (SH&F), simultaneous saccharification and co-fermentation (SS&CF), simultaneous saccharification and fermentation (SS&F), and modified simultaneous saccharification and fermentation (SS&F) techniques. Drying and wet-milling generated 41.2 g of white starch/100 g flour, and processing with alum gave 58.6/100 g MKS. Hydrolysis of 5 g MKS by sulfuric acid, sodium hydroxide, malted "acha", and Aspergillus niger amylase for 2 h produced (g/100 mL) 3.97 g, 4.0 g, 4.43 g and 4.24 g of sugar, respectively. Fermentation with 7 g of MKS yielded maximum sugar and ethanol concentrations. Ethanol obtained using SS&CF, SH&F, SS&F and modified SS&F were (v/v); 0.26%, 2.0%, 1.13% and 3.985%, respectively. These results confirmed MKS as a potential feedstock for bioethanol production.

Mango Seed Kernel Fat as a Cocoa Butter Substitute Suitable for the Tropics.

Cocoa butter is a key ingredient in many chocolate products but its partial substitution with mango (Mangifera indica L.) seed kernel fat (MSKF) has the potential to reduce chocolate production costs and improve shelf-life. Here, MSKF was extracted from three cultivars of mango grown in Pakistan: Lal Badshah, Anwar Retual, and Chaunsa. Physicochemical and antioxidant properties of the MSKF samples were studied at 0, 30, and 60 days of storage at 30 °C, a temperature reflecting typical storage conditions in the tropics. Overall, the Lal Badshah MSKF had the most favorable physicochemical properties, including the highest DPPH antioxidant activity among the three cultivars. Thus, Lal Badshah MSKF was used to formulate cocoa butter substitute chocolate (CBSC), substituting the cocoa butter at 20 to 80 g/100 g. CBSC had a lower value for hardness (3.80 N) compared with the control chocolate (4.42 N). Color values L* , a* , and b* were not significantly affected by the different rates of substitution or by length of storage. Oxidative stability and antioxidant potential of CBSC increased with both higher substitution levels of MSKF and length of storage. The results suggest that MSKF can be utilized as a cocoa butter substitute at levels up to 60 g/100 g. This potential for substitution is particularly valuable for tropical regions where refrigerated storage may not be available or financially viable. PRACTICAL APPLICATION: Mango seed kernel fat (MSKF) has potential to be used as a cocoa butter substitute in confectionery products, particularly chocolate. The mango industry could utilize fat extraction from mango seeds, which are normally a waste product, for value adding.