Multiple stressors determine river ecological status at the European scale: Towards an integrated understanding of river status deterioration.

The biota of European rivers are affected by a wide range of stressors impairing water quality and hydro-morphology. Only about 40% of Europe's rivers reach 'good ecological status', a target set by the European Water Framework Directive (WFD) and indicated by the biota. It is yet unknown how the different stressors in concert impact ecological status and how the relationship between stressors and status differs between river types. We linked the intensity of seven stressors to recently measured ecological status data for more than 50,000 sub-catchment units (covering almost 80% of Europe's surface area), which were distributed among 12 broad river types. Stressor data were either derived from remote sensing data (extent of urban and agricultural land use in the riparian zone) or modelled (alteration of mean annual flow and of base flow, total phosphorous load, total nitrogen load and mixture toxic pressure, a composite metric for toxic substances), while data on ecological status were taken from national statutory reporting of the second WFD River Basin Management Plans for the years 2010-2015. We used Boosted Regression Trees to link ecological status to stressor intensities. The stressors explained on average 61% of deviance in ecological status for the 12 individual river types, with all seven stressors contributing considerably to this explanation. On average, 39.4% of the deviance was explained by altered hydro-morphology (morphology: 23.2%; hydrology: 16.2%), 34.4% by nutrient enrichment and 26.2% by toxic substances. More than half of the total deviance was explained by stressor interaction, with nutrient enrichment and toxic substances interacting most frequently and strongly. Our results underline that the biota of all European river types are determined by co-occurring and interacting multiple stressors, lending support to the conclusion that fundamental management strategies at the catchment scale are required to reach the ambitious objective of good ecological status of surface waters.

Ecological opportunities and intraspecific competition alter trophic niche specialization in an opportunistic stream predator.

Many generalist populations are composed of specialized individuals that use a narrow part of the population's niche. Ecological theories predict that individual specialization and population trophic niche are determined by biotic interactions and resource diversity emerging from environmental variations (i.e. ecological opportunities). However, due to the paucity of empirical and experimental demonstrations, the genuine importance of each of these drivers in determining trophic niche attributes is not fully appreciated. The present study aimed at determining the population level and individual responses of brown trout (Salmo trutta) to variations in ecological opportunities (terrestrial prey inputs) and autochthonous prey communities among 10 stream reaches along a riparian condition gradient using individual longitudinal monitoring and stable isotope analyses. Our results suggested that trophic niche diversity varied along the environmental gradient, while individual trophic specialization was indirectly driven by ecological opportunities through strengthened intraspecific competition. Individual diet was repeatable over the study period, and the growth rate of juvenile brown trout increased with their specialization for aquatic predatory invertebrates. Our findings highlight the dual influences of intraspecific competition and ecological opportunities on individual trophic specialization and population trophic niche.

Riparian forests mitigate harmful ecological effects of agricultural diffuse pollution in medium-sized streams.

Agricultural pollution persists as a significant environmental problem for stream ecosystems. Uncultivated buffer zones or reforestation of riparian zones are advocated as a key management option that could compensate the harmful land use impacts. The effectiveness of riparian forests to protect ecological conditions of agricultural streams is yet inconclusive, particularly regarding the benefit of riparian buffers in streams suffering from uninterrupted agricultural diffuse pollution. We studied the effects of riparian land use on periphyton production and diatom, macrophyte and benthic macroinvertebrate communities in medium-sized agricultural streams by a) comparing 18 open field and forested agricultural stream reach pairs that only differed by the extent of riparian forest cover, and b) comparing the agricultural reaches to 15 near-natural streams. We found that periphyton abundance was higher in open reaches than in the forested reaches, but diatom community structure did not respond to the riparian forest cover. Macrophyte and macroinvertebrate communities were clearly affected by the riparian forest cover. Graminoids dominated in open reaches, whereas bryophytes were more abundant in forested reaches. Shredding invertebrates were more abundant in forested reaches compared to open reaches, but grazers did not differ between the reach types. Macrophyte trait composition and macroinvertebrate community difference between the reaches were positively related to the difference in riparian forest cover. The community structure of all three groups in the agricultural streams differed distinctly from the near-natural streams. However, only macrophyte communities in forested agricultural reaches showed resemblance to near-natural composition. Our results suggest that riparian forests provide ecological benefits that can partly compensate the impacts of agricultural diffuse pollution. However, community structure of forested agricultural reaches did not match the near-natural composition in any organism group indicating that catchment-scale management and mitigation of diffuse pollution need to be still advocated to achieve ecological goals in stream management and restoration.