RESUMEN
The gene of catechol 1, 2-dioxygenase was identified and cloned from the genome of Oceanimonas marisflavi 102-Na3. The protein was expressed in Escherichia coli BL21 (DE3) and purified to homogeneity of a dimer with molecular mass of 69.2 kDa. The enzyme was highly stable in pH 6.0-9.5 and below 45 °C and exhibited the maximum activity at pH 8.0 and 30 °C. Being the first characterized intradiol dioxygenase from marine bacteria Oceanimonas sp., the enzyme showed catalytic activity for catechol, 3-methylcatechol, 4-methylcatechol, 3-chlorocatechol, 4-chlorocatechol and pyrogallol. For catechol, Km and Vmax were 11.2 µM and 13.4 U/mg of protein, respectively. The enzyme also showed resistance to most of the metal ions, surfactants and organic solvents, being a promising biocatalyst for biodegradation of aromatic compounds in complex environments.
Asunto(s)
Aeromonadaceae/enzimología , Proteínas Bacterianas/genética , Catecol 1,2-Dioxigenasa/genética , Catecoles/metabolismo , Aeromonadaceae/química , Aeromonadaceae/clasificación , Aeromonadaceae/genética , Secuencia de Aminoácidos , Proteínas Bacterianas/química , Proteínas Bacterianas/aislamiento & purificación , Proteínas Bacterianas/metabolismo , Catecol 1,2-Dioxigenasa/química , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Catecol 1,2-Dioxigenasa/metabolismo , Catecoles/química , Clonación Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Vectores Genéticos/química , Vectores Genéticos/metabolismo , Concentración de Iones de Hidrógeno , Cinética , Peso Molecular , Filogenia , Multimerización de Proteína , Pirogalol/química , Pirogalol/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Especificidad por SustratoRESUMEN
Catechol 1,2-dioxygenases catalyze catechol ring-opening, a critical step in the degradation of aromatic compounds. Cupriavidus campinensis BJ71, an efficient 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacterial strain, was previously isolated from an environment contaminated with 2,4-D. In this study, catA encoding a catechol 1,2-dioxygenase was cloned from the BJ71 strain. The gene was 939 bp long and encoded a polypeptide of 312 amino acids with a molecular weight of 34 kDa. To investigate its enzymatic characteristics, CatA was heterologously expressed in Escherichia coli. Optimal reaction conditions for the pure enzyme were 35 °C and pH 8.0. The enzyme remained stable within a range of 25 °C-45 °C and pH 6.0-9.0, thus indicating that CatA has wide temperature and pH adaptability. After incubation at 45 °C, the enzyme activity of CatA decreased to 37.12%, but its activity was not affected by incubation at pH 9.0. The pure enzyme was able to use catechol, 4-methyl-catechol and 4-chlorocatechol as substrates. Enzyme kinetic parameters Km and Vmax were 39.97 µM and 10.68 U/mg, respectively. This is the first report of the cloning of a gene encoding a catechol 1,2-dioxygenase from a 2,4-D-degrading bacterial strain.
Asunto(s)
Proteínas Bacterianas/química , Catecol 1,2-Dioxigenasa/química , Cupriavidus/enzimología , Secuencia de Aminoácidos , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Catecol 1,2-Dioxigenasa/genética , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Clonación Molecular , Pruebas de Enzimas , Escherichia coli/genética , Concentración de Iones de Hidrógeno , Filogenia , Alineación de Secuencia , TemperaturaRESUMEN
OBJECTIVES: Candida tropicalis yeast is a microorganism that possesses high tolerance for phenol and shows strong phenol degrading activity. This yeast is capable of utilizing phenol as the sole carbon and energy source. While the enzyme participating on the first step of phenol biodegradation, NADPH-dependent phenol hydroxylase, has already been characterized, information on the enzyme participating in the second step of its degradation, catechol 1,2-dioxygenase, is scarce. The development of the procedure suitable for catechol 1,2-dioxygenase isolation and partial characterization of this enzyme are the aims of this study. METHODS: Combination of chromatography on DEAE-Sepharose and gel-permeation chromatography on Sephadex G-100 was used for isolation of cytosolic catechol 1,2-dioxygenase from C. tropicalis yeast. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and gel chromatography on Sephadex G-100 were used to evaluate the molecular mass of the enzyme. The enzyme activity was followed by HPLC (catechol consumption and/or cis,cis-muconic acid formation). RESULTS: Using the isolation procedure consisting of chromatography and re-chromatography on a column of DEAE-Sepharose and gel filtration on Sephadex G-100, catechol 1,2-dioxygenase was purified from C. tropicalis cytosol to homogeneity. Catechol 1,2-dioxygenase was found to be a homodimer with a subunit molecular mass of 30000 +/- 5000. The enzyme oxidized catechol producing cis,cis-muconic acid. The optimal temperature and pH were 30 degrees C and 7.7, respectively. CONCLUSIONS: The data are the first report showing the isolation of eukaryotic catechol 1,2-dioxygenase from C. tropicalis to homogeneity and its partial characterization.
Asunto(s)
Candida tropicalis/enzimología , Catecol 1,2-Dioxigenasa/química , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Proteínas Fúngicas/química , Proteínas Fúngicas/aislamiento & purificación , Candida tropicalis/metabolismo , Catecol 1,2-Dioxigenasa/metabolismo , Catecoles/química , Catecoles/metabolismo , Cromatografía , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Citosol/química , Citosol/enzimología , Citosol/metabolismo , Dextranos , Dimerización , Electroforesis en Gel de Poliacrilamida , Proteínas Fúngicas/metabolismo , Concentración de Iones de Hidrógeno , Oxidación-Reducción , Fenol/química , Ácido Sórbico/análogos & derivados , Ácido Sórbico/química , Ácido Sórbico/metabolismo , TemperaturaRESUMEN
The aniline-assimilating bacterium Rhodococcus sp. AN-22 was found to constitutively synthesize CatB (cis,cis-muconate cycloisomerase) and CatC (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported CatA (catechol 1,2-dioxygenase). The bacterium maintained the specific activity of the three enzymes at an almost equal level during cultivation on succinate. CatB and CatC were purified to homogeneity and characterized. CatB was a monomer with a molecular mass of 44 kDa. The enzyme was activated by Mn2+, Co2+ and Mg2+. Native CatC was a homo-octamer with a molecular mass of 100 kDa. The enzyme was stable between pH 7.0 and 10.5 and was resistant to heating up to 90 degrees C. Genes coding for CatA, CatB and CatC were cloned and named catA, catB and catC respectively. The catABC genes were transcribed as one operon. The deduced amino acid sequences of CatA, CatB and CatC showed high identities with those from other Gram-positive micro-organisms. A regulator gene such as catR encoding a regulatory protein was not observed around the cat gene cluster of Rhodococcus sp. AN-22, but a possible relic of catR was found in the upstream region of catA. Reverse transcriptase-PCR and primer extension analyses showed that the transcriptional start site of the cat gene cluster was located 891 bp upstream of the catA initiation codon in the AN-22 strain growing on both aniline and succinate. Based on these data, we concluded that the bacterium constitutively transcribed the catABC genes and translated its mRNA into CatA, CatB and CatC.
Asunto(s)
Compuestos de Anilina/metabolismo , Proteínas Bacterianas , Regulación Bacteriana de la Expresión Génica , Genes Bacterianos/genética , Rhodococcus/genética , Rhodococcus/metabolismo , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Proteínas Bacterianas/metabolismo , Catecol 1,2-Dioxigenasa/biosíntesis , Catecol 1,2-Dioxigenasa/genética , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Catecol 1,2-Dioxigenasa/metabolismo , Isomerasas/genética , Isomerasas/metabolismo , Datos de Secuencia Molecular , Rhodococcus/enzimología , Especificidad por Sustrato , Sitio de Iniciación de la TranscripciónRESUMEN
This study intends to purify and characterize catechol 1,2-dioxygenase (C1,2O) of phenol-degrading Acinetobacter sp. Y64 and of E. coli transformant. Acinetobacter sp. Y64 was capable of degrading 1000 mg/L of phenol within 14 ± 2 h at 30 °C, 160 rpm and pH of 7. One C1,2O of 36 kDa was purified using ammonium sulphate precipitation and Hitrap QFF column chromatograph with 49% recovery and a 10.6-fold increase in purity. Purified Y64 C1,2O had temperature and pH optimum at 37 °C and pH 7.7 respectively with the Michaelis constant of 17.53 µM and the maximal velocity of 1.95 U/mg, respectively. The presence of Fe(3+) or Fe(2+) enhanced the activity of Y64 C1,2O while other compounds such as Ca(2+), and EDTA had an inhibitory effect. 80% of C1,2O activity remained using 4-nitrocatechol as substrate while 2% remained using 3-methylcatechol compared with that using catechol. Y64 catA gene encoding C1,2O was amplified using PCR cloned into pET22b vector and expressed in Escherichia coli BL21 DE3 (pLysS) after transformation. Purified and cloned Y64 C1,2O show no significant differences in the biochemical properties. The phylogenetic tree based on the protein sequences indicates that these C1,2Os possess a common ancestry.
Asunto(s)
Acinetobacter/enzimología , Proteínas Bacterianas/aislamiento & purificación , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Proteínas Recombinantes/aislamiento & purificación , Acinetobacter/genética , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Catecol 1,2-Dioxigenasa/química , Catecol 1,2-Dioxigenasa/genética , Catecol 1,2-Dioxigenasa/metabolismo , Clonación Molecular , Escherichia coli/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismoRESUMEN
The enzymatic decomposition of 4-chlorophenol metabolites using an immobilized biocatalyst was investigated in this study. Catechol 1,2-dioxygenase for ortho ring cleavage obtained via cloning of the corresponding gene cphA-I from Arthrobacter chlorophenolicus A6 was overexpressed and purified. It was found that the cphA-I enzyme could catalyze the degradation of catechol, 4-chlorocatechol, and 3-methylcatechol. The expressed enzyme was immobilized onto a natural enzyme support, fulvic acid-activated montmorillonite. The immobilization yield was as high as 63%, and the immobilized enzyme maintained high substrate utilization activity, with only a 15-24% reduction in the specific activity. Kinetic analysis demonstrated marginal differences in νmax and KM values for the free and immobilized enzymes, indicating that inactivation of the immobilized enzyme was minimal. The immobilized enzyme exhibited notably increased stability against changes in the surrounding environment (temperature, pH, and ionic strength). Our results provide useful information for the effective enzymatic biochemical treatment of hazardous organic compounds.
Asunto(s)
Catecol 1,2-Dioxigenasa/química , Clorofenoles/química , Hidrocarburos Clorados/química , Purificación del Agua/métodos , Arthrobacter/enzimología , Arthrobacter/genética , Secuencia de Bases , Catecol 1,2-Dioxigenasa/genética , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Clorofenoles/análisis , Clonación Molecular , Estabilidad de Enzimas , Enzimas Inmovilizadas/química , Hidrocarburos Clorados/análisis , Cinética , Datos de Secuencia MolecularRESUMEN
In vitro degradation of pyrene was studied in MSM by three bacterial strains individually, designated as BP10, NJ2 and P2. Among these strains, NJ2 was the highest degrader (60%) of pyrene, followed by BP10 (44%) and the least was P2 (42%) in MSM with pyrene (50 µg ml(-1)) in 8 days. During pyrene degradation, catechol 1,2 dioxygenase (C12O) activity was induced by 13 folds in BP10 and 17 folds in P2 as compared to catechol 2,3 dioxygenase (C23O). However, in NJ2, C23O activity was augmented 1.3 times more than C12O. This clearly indicated that C12O played a major role in pyrene degradation by BP10 and P2, while in NJ2, C23O contributed more to degradation process than C12O. Molecular weight of highly inducible C12O was determined as ~64 kDa by size exclusion chromatography and as ~32 kDa on denaturing SDS PAGE in BP10 which indicated dimeric nature of the enzyme.
Asunto(s)
Bacterias/efectos de los fármacos , Bacterias/enzimología , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Catecol 2,3-Dioxigenasa/aislamiento & purificación , Medios de Cultivo/farmacología , Pirenos/metabolismo , Bacterias/genética , Bacterias/crecimiento & desarrollo , Proteínas Bacterianas/metabolismo , Secuencia de Bases , Biodegradación Ambiental/efectos de los fármacos , Catecol 1,2-Dioxigenasa/química , Catecol 2,3-Dioxigenasa/química , Electroforesis en Gel de Poliacrilamida , Concentración de Iones de Hidrógeno/efectos de los fármacos , Datos de Secuencia Molecular , Peso Molecular , Oxidación-Reducción/efectos de los fármacos , FilogeniaAsunto(s)
Proteínas Bacterianas/metabolismo , Catecol 1,2-Dioxigenasa/metabolismo , Catecoles/metabolismo , Hidroxibenzoatos/farmacología , Rhodococcus/efectos de los fármacos , Proteínas Bacterianas/genética , Proteínas Bacterianas/aislamiento & purificación , Catecol 1,2-Dioxigenasa/genética , Catecol 1,2-Dioxigenasa/aislamiento & purificación , Catecoles/química , Activación Enzimática/efectos de los fármacos , Pruebas de Enzimas , Expresión Génica , Cinética , Rhodococcus/enzimología , Rhodococcus/genética , Fracciones Subcelulares/química , Fracciones Subcelulares/metabolismo , Especificidad por SustratoRESUMEN
A eukaryotic catechol 1,2-dioxygenase (1,2-CTD) was produced from a Candida albicans TL3 that possesses high tolerance for phenol and strong phenol degrading activity. The 1,2-CTD was purified via ammonium sulfate precipitation, Sephadex G-75 gel filtration, and HiTrap Q Sepharose column chromatography. The enzyme was purified to homogeneity and found to be a homodimer with a subunit molecular weight of 32,000. Each subunit contained one iron. The optimal temperature and pH were 25 degrees C and 8.0, respectively. Substrate analysis showed that the purified enzyme was a type I catechol 1,2-dioxygenase. This is the first time that a 1,2-CTD from a eukaryote (Candida albicans) has been characterized. Peptide sequencing on fragments of 1,2-CTD by Edman degradation and MALDI-TOF/TOF mass analyses provided information of amino acid sequences for BLAST analysis, the outcome of the BLAST revealed that this eukaryotic 1,2-CTD has high identity with a hypothetical protein, CaO19_12036, from Candida albicans SC5314. We conclude that the hypothetical protein is 1,2-CTD.