RESUMEN
A light-driven proton pump bacteriorhodopsin (bR) forms a two-dimensional hexagonal lattice with about 10 archaeal lipids per monomer bR on purple membrane (PM) of Halobacterium salinarum. In this study, we found that the weakening of the bR-lipid interaction on PM by addition of alcohol can be detected as the significant increase of protein solubility in a nonionic detergent, dodecyl ß-D-maltoside (DDM). The protein solubility in DDM was also increased by bR-lipid interaction change accompanied by structural change of the apoprotein after retinal removal and was about 7 times higher in the case of completely bleached membrane than that of intact PM. Interestingly, the cyclic and milliseconds order of structural change of bR under light irradiation also led to increasing the protein solubility and had a characteristic light intensity dependence with a phase transition. These results indicate that there is a photointermediate in which bR-lipid interaction has been changed by its dynamic structural change. Because partial delipidation of PM by CHAPS gave minor influence for the change of the protein solubility compared to intact PM in both dark and light conditions, it is suggested that specific interactions of bR with some lipids which remain on PM even after delipidation treatment have a key role for the change of solubility in DDM induced by alcohol binding, ligand release, and photon absorption on bR.
Asunto(s)
Bacteriorodopsinas/metabolismo , Detergentes/química , Glucósidos/química , Metabolismo de los Lípidos , Alcoholes/farmacología , Bacteriorodopsinas/química , Halobacterium salinarum/citología , Luz , Metabolismo de los Lípidos/efectos de los fármacos , Metabolismo de los Lípidos/efectos de la radiación , Unión Proteica/efectos de los fármacos , Unión Proteica/efectos de la radiación , Membrana Púrpura/efectos de los fármacos , Membrana Púrpura/metabolismo , Membrana Púrpura/efectos de la radiación , Solubilidad/efectos de los fármacos , Solubilidad/efectos de la radiaciónRESUMEN
Indolicidin, a cationic antimicrobial tridecapeptide amide, is rich in proline and tryptophan residues. Its biological activity is intensively studied, but the details how indolicidin interacts with membranes are not fully understood yet. We report here an in situ atomic force microscopic study describing the effect of indolicidin on an artificial supported planar bilayer membrane of dipalmitoyl phosphatidylcholine (DPPC) and on purple membrane of Halobacterium salinarum. Concentration dependent interaction of the peptide and membranes was found in case of DPPC resulting the destruction of the membrane. Purple membrane was much more resistant against indolicidin, probably due to its high protein content. Indolicidin preferred the border of membrane disks, where the lipids are more accessible. These data suggest that the atomic force microscope is a powerful tool in the study of indolicidin-membrane interaction.
Asunto(s)
1,2-Dipalmitoilfosfatidilcolina/análisis , Péptidos Catiónicos Antimicrobianos/administración & dosificación , Membrana Púrpura/efectos de los fármacos , Membrana Púrpura/ultraestructura , Antiinfecciosos/administración & dosificación , Antiinfecciosos/análisis , Péptidos Catiónicos Antimicrobianos/análisis , Relación Dosis-Respuesta a Droga , Halobacterium salinarum/metabolismo , Membrana Dobles de Lípidos/análisis , Microscopía de Fuerza Atómica/métodosRESUMEN
Glycocardiolipin is an archaeal analogue of mitochondrial cardiolipin, having an extraordinary affinity for bacteriorhodopsin, the photoactivated proton pump in the purple membrane of Halobacterium salinarum. Here purple membranes have been isolated by osmotic shock from either cells or envelopes of Hbt. salinarum. We show that purple membranes isolated from envelopes have a lower content of glycocardiolipin than standard purple membranes isolated from cells. The properties of bacteriorhodopsin in the two different purple membrane preparations are compared; although some differences in the absorption spectrum and the kinetic of the dark adaptation process are present, the reduction of native membrane glycocardiolipin content does not significantly affect the photocycle (M-intermediate rise and decay) as well as proton pumping of bacteriorhodopsin. However, interaction of the pumped proton with the membrane surface and its equilibration with the aqueous bulk phase are altered.
Asunto(s)
Bacteriorodopsinas/fisiología , Cardiolipinas/administración & dosificación , Halobacterium salinarum/fisiología , Bombas de Protones/fisiología , Membrana Púrpura/efectos de los fármacos , Membrana Púrpura/fisiología , Bacteriorodopsinas/efectos de los fármacos , Bacteriorodopsinas/efectos de la radiación , Relación Dosis-Respuesta a Droga , Halobacterium salinarum/efectos de los fármacos , Halobacterium salinarum/efectos de la radiación , Luz , Bombas de Protones/efectos de los fármacos , Bombas de Protones/efectos de la radiación , Protones , Propiedades de SuperficieRESUMEN
To investigate the effects of temperature and hydration on the dynamics of purple membrane (PM), we measured the broadband complex dielectric spectra from 0.5 GHz to 2.3 THz using a vector network analyzer and terahertz time-domain spectroscopy from 233 to 293 K. In the lower temperature region down to 83 K, the complex dielectric spectra in the THz region were also obtained. The complex dielectric spectra were analyzed through curve fitting using several model functions. We found that the hydrated states of one relaxational mode, which was assigned as the coupled motion of water molecules with the PM surface, began to overlap with the THz region at approximately 230 K. On the other hand, the relaxational mode was not observed for the dehydrated state. On the basis of this result, we conclude that the protein-dynamical-transition-like behavior in the THz region is due to the onset of the overlap of the relaxational mode with the THz region. Temperature hysteresis was observed in the dielectric spectrum at 263 K when the hydration level was high. It is suggested that the hydration water behaves similarly to supercooled liquid at that temperature. The third hydration layer may be partly formed to observe such a phenomenon. We also found that the relaxation time is slower than that of a globular protein, lysozyme, and the microscopic environment in the vicinity of the PM surface is suggested to be more heterogeneous than lysozyme. It is proposed that the spectral overlap of the relaxational mode and the low-frequency vibrational mode is necessary for the large conformational change of protein.
Asunto(s)
Simulación de Dinámica Molecular , Membrana Púrpura/química , Membrana Púrpura/efectos de los fármacos , Temperatura , Agua/farmacología , Espectroscopía Dieléctrica , Halobacterium salinarum/química , Espectroscopía de Terahertz , Agua/químicaRESUMEN
Earlier it was an orthodoxy that purple membranes (PMs) in aqueous medium are shaped as flat hard disks. In a few newer articles it has been shown that PMs are bent and their curvature varies with surface charge density. The purpose of this work is to answer which is the dominant factor for PM bending--structural or electrostatic forces. Two positively charged proteins are used: phytohemagglutinin (PhHA) and protamine. The electrophoretic mobility and electric polarizability of PMs are measured by microelectrophoresis and electric dichroism. The results show that both proteins reduce the mobility because they are adsorbed on PM surface. However, their influence on the electric polarizability is in the opposite direction--protamine reduces it (trivial effect) while PhHA increases the polarizability (non-trivial effect). The last result is explained by a straightening the initially bent PM because of specific bonding of PhHA to asymmetrically disposed glycolipids of PM in contrast to the electrostatic adsorption of protamine. It has been concluded that PMs in water medium are bent in the same manner as in in vivo--the intracellular surface with a higher negative charge is concave. The results indicate that electrostatic forces play a significant role in PM curvature but the shape of structural elements is the main factor determining the geometry of PM.
Asunto(s)
Protaminas/farmacocinética , Membrana Púrpura/química , Adsorción , Centrifugación , Dicroismo Circular , Relación Dosis-Respuesta a Droga , Electroquímica/métodos , Electroforesis , Ensayo de Cambio de Movilidad Electroforética , Glucolípidos/química , Concentración de Iones de Hidrógeno , Concentración Osmolar , Phaseolus/citología , Lectinas de Plantas/metabolismo , Lectinas de Plantas/farmacología , Protaminas/química , Membrana Púrpura/efectos de los fármacos , Membrana Púrpura/metabolismo , Sonicación , Espectrofotometría , Electricidad Estática , Suspensiones/química , Agua/químicaRESUMEN
Bacteriorhodopsin (bR) is the prototype of an integral membrane protein with seven membrane-spanning alpha-helices and serves as a model of the G-protein-coupled drug receptors. This study is aimed at reaching a greater understanding of the role of amine local anesthetic cations on the proton transport in the bR protein, and furthermore, the functional role of "the cation" in the proton pumping mechanism. The effect of the amine anesthetic cations on the proton pump in the bR blue membrane was compared with those by divalent (Ca2+, Mg2+ and Mn2+) and monovalent metal cations (Li+, Na+, K+ and Cs+), which are essential for the correct functioning of the proton pumping of the bR protein. The results suggest that the interacting site of the divalent cation to the bR membrane may differ from that of the monovalent metal cation. The electric current profile of the bR blue membrane in the presence of the amine anesthetic cations was biphasic, involving the generation and inhibition of the proton pumping activity in a concentration-dependent manner. The extent of the regeneration of the proton pump by the additives increased in the order of monovalent metal cationAsunto(s)
Aminas/farmacología
, Anestésicos/farmacología
, Bacteriorodopsinas/metabolismo
, Membrana Celular/efectos de los fármacos
, Bacteriorodopsinas/antagonistas & inhibidores
, Cationes
, Halobacterium salinarum/efectos de los fármacos
, Inhibidores de la Bomba de Protones
, Protones
, Membrana Púrpura/efectos de los fármacos
RESUMEN
We quantitatively assessed the spectroscopic changes of purple membrane in relation to the concentrations of a volatile anesthetic. As reported previously, volatile anesthetics show three modes of action on purple membrane. By using an anesthetic for which the concentration in solution could be determined spectroscopically and by applying modified analytical methods regarding the M-intermediate lifetime, we were able to clarify the quantitative relation between anesthetic concentration and each mode of action, a relation which in the past has only been described qualitatively. We also determined through the measurement of transient pH changes with pyranine that the proton pump efficiency per photochemical cycle in an action mode induced with low concentrations of anesthetic does not change from that of the native state. Moreover, we dynamically obtained the individual M-bacteriorhodopsin difference spectrum of each state at room temperature using our flash photolysis system equipped with a wavelength-tunable dye laser. These results demonstrated again that we should clearly distinguish different action modes of anesthetics according to their concentrations.
Asunto(s)
Anestésicos/farmacología , Membrana Púrpura/efectos de los fármacos , Bacteriorodopsinas/química , Cloroformo/farmacología , Relación Dosis-Respuesta a Droga , Concentración de Iones de Hidrógeno , Rayos Láser , Metoxiflurano/farmacología , Éteres Metílicos/farmacología , Fotólisis , Bombas de Protones/química , Membrana Púrpura/química , Sevoflurano , Espectrofotometría , TemperaturaRESUMEN
A high resolution projection at 2.6 A of deoxycholate-treated purple membrane using only images has been obtained with a 200 keV FEG microscope operated at liquid helium temperature. Examination of this high quality map has allowed the following conclusions to be made: Comparison between the internal structure of the trimers of the native and the deoxycholate-treated crystal forms shows that almost every detail of the structure at high resolution is identical. The cell dimension change from 62.4 A to 57.9 A is accompanied by a loss of about half the normal lipids and a 2 degrees anticlockwise rotation of the trimer as a rigid body. Three of the lipids per bacteriorhodopsin molecule remain in identical positions relative to the trimer. In addition, from the projection map together with a packing analysis using the atomic model for bacteriorhodopsin, space for three further lipids has been identified making a total of six lipids per bacteriorhodopsin molecule in this crystal form. Finally, the surprisingly small rotation of the trimer between the two crystal forms with completely different Van der Waals contacts suggest that the crystals are held together by strong, long-range electrostatic interactions.
Asunto(s)
Bacteriorodopsinas/química , Ácido Desoxicólico/farmacología , Lípidos/análisis , Membrana Púrpura/química , Bacteriorodopsinas/ultraestructura , Cristalización , Cristalografía , Cristalografía por Rayos X , Electrones , Análisis de Fourier , Glucosa , Conformación Molecular , Conformación Proteica , Membrana Púrpura/efectos de los fármacos , Membrana Púrpura/ultraestructura , Adhesión del Tejido , AguaRESUMEN
Structural changes of purple membrane during photobleaching in the presence of hydroxylamine were monitored using atomic force microscopy (AFM). The process of bleaching was associated with the disassembly of the purple membrane crystal into smaller crystals. Imaging steps of the photobleaching progress showed that disassembly proceeds until the sample is fully bleached and its crystallinity is almost lost. As revealed from high resolution AFM topographs, the loss of crystallinity was initiated by loss of lattice forming contact between the individual bacteriorhodopsin trimers. The bacteriorhodopsin molecules, however, remained assembled into trimers during the entire photobleaching process. Regeneration of the photobleached sample into intact purple membrane resulted in the reassembly of the bacteriorhodopsin trimers into the trigonal lattice of purple membrane. The data provide novel insights into factors triggering purple membrane formation and structure.
Asunto(s)
Halobacterium salinarum/citología , Hidroxilamina/metabolismo , Microscopía de Fuerza Atómica , Membrana Púrpura/metabolismo , Membrana Púrpura/ultraestructura , Bacteriorodopsinas/química , Bacteriorodopsinas/metabolismo , Bacteriorodopsinas/ultraestructura , Cristalización , Halobacterium salinarum/ultraestructura , Hidroxilamina/farmacología , Procesamiento de Imagen Asistido por Computador , Unión Proteica/efectos de los fármacos , Estructura Cuaternaria de Proteína/efectos de los fármacos , Membrana Púrpura/química , Membrana Púrpura/efectos de los fármacosRESUMEN
The M intermediate decay in the photocycle of D96N mutant bacteriorhodopsin does not depend on the light intensity of the exciting flash. Cooperative phenomena in the photocycle are revealed after addition of azide causing acceleration of the M decay and making it kinetically well separated from the N decay. Increase in the light intensity induces slight deceleration of the M decay and significant acceleration of the N decay. The data obtained directly confirm our recent model [Komrakov and Kaulen (1995) Biophys. Chem. 56, 113-119], according to which appearance of the Mslow intermediate in the photocycle of the wild type bR at high light intensity is due to destabilization of the N intermediate leading to the acceleration of the N-->M and N-->bR reactions.
Asunto(s)
Bacteriorodopsinas/metabolismo , Halobacterium salinarum , Membrana Púrpura/metabolismo , Regulación Alostérica , Azidas/farmacología , Bacteriorodopsinas/efectos de los fármacos , Bacteriorodopsinas/efectos de la radiación , Transporte Biológico , Cinética , Mutación , Protones , Membrana Púrpura/efectos de los fármacos , Membrana Púrpura/efectos de la radiación , Azida Sódica , EspectrofotometríaRESUMEN
Using steady-state spectroscopic and zeta potential methods, we have unraveled the interaction of the purple membrane (PM) and bacteriorhodopsin (bR) with various surfactants below their critical micelle concentrations. We found that the charged hydrophilic heads of ionic surfactants play a role in perturbing the structure and conformation of PM and bR and that ionic surfactants of opposite charges cause opposing effects. Specifically, the addition of a low concentration (0.2 mM) of the cationic surfactant cetyl trimethylammonium bromide (CTAB) is capable of neutralizing the negatively charged lipids on the PM surface via electrostatic forces. This results in increased hydrophobicity of PM that leads to the aggregation of PM. In contrast, denaturation of PM and bR was observed when the anionic surfactant sodium dodecyl sulfate (SDS) was added to the PM suspensions. The attachment of SDS to the PM surface increases the solubility of PM and causes a loose crystalline structure. As the SDS concentration is increased to more than 3 mM, the secondary structure of the constituents of bR is significantly distorted, and the protonated Schiff base is hydrolyzed to form free retinal. The addition of the neutral surfactant diethylene glycol mono-n-hexyl ether (C6E2) does not significantly influence the PM and bR, meaning most of their original properties are preserved. We conclude that the addition of surfactants might cause the aggregation or solubilization of the membrane protein, depending on the signs of the charged hydrophilic heads of the surfactants and the charges of the membrane protein surface. Aggregation results when the surfactant and protein have opposite charges, whereas solubilization results when the surfactant and protein have the same charge.
Asunto(s)
Bacteriorodopsinas/química , Multimerización de Proteína/efectos de los fármacos , Membrana Púrpura/efectos de los fármacos , Tensoactivos/farmacología , Interacciones Hidrofóbicas e Hidrofílicas , Modelos Moleculares , Estructura Cuaternaria de Proteína , Solubilidad/efectos de los fármacos , Electricidad EstáticaRESUMEN
Protection of biological compounds, for example, enzymes, viruses, or even whole cells, against degradation is very important for many applications. Embedding of such compounds into polymer matrices is a straightforward common method. However, in biotechnology and medicine there is a great interest to prepare micro- and nanosized shells around the biocomponents in order to protect them and having only a minor increase in size. The PEGylation of biological macromolecules has gained attention because degradation by proteolytic enzymes is significantly retarded and, in turn, their bioavailability is enhanced. We found that PEGylation is also a powerful tool to protect biomaterials from degradation by small organic solvent molecules, in particular, ethanol. Methoxy-polyethylene glycol (MPEG) modified BR survives exposure to significant concentrations of ethanol, up to 30%, and preserves its photochromism, whereas unmodified PM is instantaneously denatured at such concentrations. This is useful for potential technical applications of BR but is of relevance for many other applications where biomaterials and, in particular, biomembranes may be exposed to solvents.
Asunto(s)
Bacteriorodopsinas/química , Etanol/farmacología , Polietilenglicoles/química , Secuencia de Aminoácidos , Bacteriorodopsinas/metabolismo , Relación Dosis-Respuesta a Droga , Halobacterium salinarum/citología , Datos de Secuencia Molecular , Estabilidad Proteica/efectos de los fármacos , Membrana Púrpura/efectos de los fármacos , Membrana Púrpura/metabolismoRESUMEN
Carboxyl groups of bacteriorhodopsin (bR) that are modified by 1-ethyl-3-[3-(trimethylamino)-propyl]carbodiimide (ETC) have been identified. Reaction of deionized purple membrane with a 400-fold molar excess of ETC or [14C]ETC for 1 h at 0 degree C incorporates about 3.5 mol of ETC/mol of bR. Proteinase K cleavage of ETC-modified bacterioopsin (bO) produced small 14C-labeled peptides. Amino acid sequence analysis showed three major ETC-modified residues: Glu 234, Asp 38, and Glu 74. Proteolysis of purple membrane with papain removes the ETC site at Glu 234. Treatment of ETC-modified, papain-cleaved purple membrane with hydroxylamine removes half of the remaining ETC label. Subsequent cleavage with chymotrypsin, followed by amino acid sequence analysis, revealed that most of the remaining label was at Glu 74. bR modified by ETC primarily at Glu 74 displays two alterations in the retinal chromophore, located in the membrane interior at a distance more than 2 nm away from the modified carboxyl group. (1) The acid-induced purple-to-blue transition undergoes a shift in apparent pK from 3.2 to 2.3. (2) The second-order rate constant for chromophore regeneration from bO and retinal is diminished from 3600 to 1700 M-1 s-1 in membrane sheets. Most of the shift in the pK of the purple-to-blue transition can be explained by the quaternary ammonium ion of ETC attached to Glu 74 overlapping the postulated location of the guanidinium group of Arg 82.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Bacteriorodopsinas/química , Membrana Púrpura/química , Retinaldehído/química , Secuencia de Aminoácidos , Aminoácidos/análisis , Carbodiimidas/farmacología , Ácidos Carboxílicos/química , Halobacterium salinarum , Cinética , Modelos Químicos , Modelos Moleculares , Datos de Secuencia Molecular , Fragmentos de Péptidos/química , Membrana Púrpura/efectos de los fármacos , Análisis de SecuenciaRESUMEN
Exposure of purple membrane from Halobacterium halobium to sublytic concentrations of Triton X-100 results in significant changes in the bacteriorhodopsin (BR) photocycle (Mukhopadhyay et al., 1994). Infrared spectra of purple membrane samples exposed briefly to Triton indicate that this change in protein function accompanies the preferential release of purple membrane glycolipids and squalenes, an association of Triton with purple membrane, and a perturbation of specific lipid headgroup interactions within the membrane. Specifically, the bilayer alterations induced by Triton entail a disruption of lipid headgroup hydrogen bonding in addition to protein conformational changes involving a loss in beta-turn and alphaII-helical structures in BR. We propose that the purple membrane glycolipids and squalenes are critical for the normal functioning of the BR photocycle and that perturbations of these lipids cause the profound photocycle changes induced by exposure to Triton. Lipid reconstitution studies demonstrated that although several of the infrared spectral parameters characteristic of the structural changes induced by Triton were reversed, the photocycle characteristics of BR in native purple membrane were not regained. The observed changes in the vibrational spectra induced by lipid-mediated bilayer perturbations suggest a useful approach for clarifying structure-function relationships of intrinsic membrane proteins exhibiting transmembrane helices.
Asunto(s)
Bacteriorodopsinas/química , Halobacterium salinarum/química , Lípidos de la Membrana/química , Membrana Púrpura/química , Bacteriorodopsinas/efectos de los fármacos , Bacteriorodopsinas/efectos de la radiación , Detergentes/farmacología , Membrana Dobles de Lípidos/química , Octoxinol/farmacología , Fotoquímica , Conformación Proteica/efectos de los fármacos , Estructura Secundaria de Proteína/efectos de los fármacos , Membrana Púrpura/efectos de los fármacos , Espectrofotometría InfrarrojaRESUMEN
The kinetics of the bacteriorhodopsin photocycle, measured by voltage changes in a closed membrane system using the direct electrometrical method (DEM) of Drachev, L.A., Jasaitus, A.A., Kaulen, A.D., Kondrashin, A.A., Liberman, E.A., Nemecek, I.B., Ostroumov, S.A., Semenov, Yu, A. & Skulachev, V.P. (1974) Nature 249, 321-324 are sixfold slower than the kinetics obtained in optical studies with suspensions of purple membrane patches. In this study, we have investigated the reasons for this discrepancy. In the presence of the uncouplers carbonyl cyanide m-chlorophenylhydrazone or valinomycin, the rates in the DEM system are similar to the rates in suspensions of purple membrane. Two alternative explanations for the effects of uncouplers were evaluated: (a) the 'back-pressure' of the Deltamicro;H+ slows the kinetic steps leading to its formation, and (b) the apparent difference between the two systems is due to slow major electrogenic events that produce little or no change in optical absorbance. In the latter case, the uncouplers would decrease the RC time constant for membrane capacitance leading to a quicker discharge of voltage and concomitant decrease in photocycle turnover time. The experimental results show that the primary cause for the slower kinetics of voltage changes in the DEM system is thermodynamic back-pressure as described by Westerhoff, H.V. & Dancshazy, Z. (1984) Trends Biochem. Sci. 9, 112-117.