RESUMEN
In earlier studies, we found that a late gene product, glycoprotein B (gB) was highly expressed in lymphoid tissues of chickens inoculated with turkey herpesvirus (HVT). The objectives of the present study were twofold. First, we wanted to expand on our previous research and determine if gB expression declines or disappears during later time periods of HVT infection. Second, we wanted to correlate gB expression with presence of HVT, i.e. if gB expression is absent, can HVT still be detected? Fifteen 1-day-old chicks were inoculated by intraperitoneal inoculation with 2000 plaque forming units of strain FC126 HVT. Thymus, spleen, bursa, brachial plexus, sciatic plexus, and feather tips were harvested at 21, 28, 35, 70, and 105 days postinoculation (PI). Brachial plexus and sciatic plexus were examined at 21, 28, and 35 days PI, and feather tips were examined at 21 and 28 days PI. An indirect immunofluorescence assay was used to detect HVT gB expression, and an in situ hybridization assay was used to detect HVT. At 21 days PI, gB expression was present in the thymus, spleen, and bursa. At 28 and 35 days PI, gB expression was detected in the thymus and spleen. At 70 days PI, gB expression was detected only in the spleen, and at 105 days PI, gB expression was not detected in any of the lymphoid tissue (thymus, spleen, or bursa). gB expression was not detected in the brachial plexus, sciatic plexus, or feather tips at any of the five time points. The bursa contained HVT only at 21 and 28 days PI. However, HVT was demonstrated in all other tissues from 21 to 105 days PI. Progression from a productive HVT infection to a latent HVT infection results in the loss of gB expression. Throughout this progression, a region of the HVT genome can be detected by appropriate methods.
Asunto(s)
Pollos , Infecciones por Herpesviridae/veterinaria , Herpesviridae/aislamiento & purificación , Tejido Linfoide/microbiología , Enfermedades de las Aves de Corral/microbiología , Animales , Plexo Braquial/metabolismo , Plexo Braquial/microbiología , Bolsa de Fabricio/metabolismo , Bolsa de Fabricio/microbiología , Plumas/metabolismo , Plumas/microbiología , Regulación Viral de la Expresión Génica , Glicoproteínas/biosíntesis , Glicoproteínas/genética , Herpesviridae/genética , Herpesviridae/metabolismo , Infecciones por Herpesviridae/microbiología , Hibridación in Situ/veterinaria , Plexo Lumbosacro/metabolismo , Plexo Lumbosacro/microbiología , Tejido Linfoide/metabolismo , ARN Viral/análisis , Bazo/metabolismo , Bazo/microbiología , Timo/metabolismo , Timo/microbiología , Pavos , Proteínas del Envoltorio Viral/biosíntesis , Proteínas del Envoltorio Viral/genéticaAsunto(s)
Leucosis Aviar/microbiología , Plexo Braquial/microbiología , Infecciones por Herpesviridae/microbiología , Infecciones por Herpesviridae/veterinaria , Herpesviridae , Neoplasias del Sistema Nervioso Periférico/microbiología , Neoplasias del Sistema Nervioso Periférico/veterinaria , Enfermedades de las Aves de Corral/microbiología , Nervio Ciático/microbiología , Animales , Pollos , Cuerpos de Inclusión Viral , Linfocitos/microbiología , Microscopía Electrónica , Células de Schwann/microbiologíaRESUMEN
We report a 15-day-old neonate with a brachial plexus neuropathy diagnosed as neuralgic amyotrophy concomitant with an osteomyelitis of the ipsilateral humerus. She was treated with intravenous antibiotics and oral dexamethasone and improved dramatically within days.
Asunto(s)
Antibacterianos/uso terapéutico , Antiinflamatorios/uso terapéutico , Neuritis del Plexo Braquial/tratamiento farmacológico , Plexo Braquial/patología , Dexametasona/uso terapéutico , Osteomielitis/tratamiento farmacológico , Antibacterianos/administración & dosificación , Antiinflamatorios/administración & dosificación , Plexo Braquial/microbiología , Neuritis del Plexo Braquial/diagnóstico , Neuritis del Plexo Braquial/patología , Dexametasona/administración & dosificación , Femenino , Humanos , Húmero/patología , Húmero/fisiopatología , Lactante , Osteomielitis/complicaciones , Resultado del TratamientoRESUMEN
Acquired brachial-plexus neuropathy outside the immediate neonatal period is uncommon. Pseudopalsy of a limb, associated with osteomyelitis, is well recognized. Acquired brachial-plexus neuropathy as the initial presentation of osteomyelitis of the humerus in the neonatal period is described. Three infants presented at 3, 15, and 21 days respectively, with acute monoplegia consistent with brachial-plexus neuropathy. The infants were afebrile and generally well. Initial radiographs of the humerus were normal and blood cultures grew group-B streptococcus in all infants. Nerve conduction studies were consistent with brachial-plexus neuropathy. Following intravenous antibiotics, there was complete recovery in all infants. Osteomyelitis of the humerus should be considered in infants in whom there are no overt signs of sepsis and who present with brachial-plexus neuropathy. Early diagnosis and appropriate treatment should result in a complete neurological recovery.