RESUMEN
This work presents a new user-friendly lyophilization simulation and process optimization tool, freely available under the name LyoPRONTO. This tool comprises freezing and primary drying calculators, a design-space generator, and a primary drying optimizer. The freezing calculator performs 0D lumped capacitance modeling to predict the product temperature variation with time which shows reasonably good agreement with experimental measurements. The primary drying calculator performs 1D heat and mass transfer analysis in a vial and predicts the drying time with an average deviation of 3% from experiments. The calculator is also extended to generate a design space over a range of chamber pressures and shelf temperatures to predict the most optimal setpoints for operation. This optimal setpoint varies with time due to the continuously varying product resistance and is taken into account by the optimizer which provides varying chamber pressure and shelf temperature profiles as a function of time to minimize the primary drying time and thereby, the operational cost. The optimization results in 62% faster primary drying for 5% mannitol and 50% faster primary drying for 5% sucrose solutions when compared with typical cycle conditions. This optimization paves the way for the design of the next generation of lyophilizers which when coupled with accurate sensor networks and control systems can result in self-driving freeze dryers.
Asunto(s)
Química Farmacéutica/métodos , Manitol/síntesis química , Sacarosa/síntesis química , Desecación/métodos , Liofilización/métodos , Congelación , Calor , TemperaturaRESUMEN
Sucrose is a widespread carbohydrate in nature and is involved in many biological processes. Its natural abundance makes it a very appealing renewable raw material for the synthetic production of high-valued molecules. To further diversify the structure and the inherent properties of these molecules, the access to sucrose analogs is of utmost interest and has historically been widely explored through chemical means. Nature also offers a large panel of sucrose-scaffold derivatives, including phosphorylated or highly substituted phenylpropanoid esters amenable to transformation. Additionally, the use of microorganisms or enzymes could provide an alternative ecologically-compatible manner to diversify sucrose-scaffold derivatives to enable the synthesis of oligo- or polysaccharides, glycoconjugates or polymers that could exhibit original properties for biotechnological applications. This review covers the main biological routes to sucrose derivatives or analogs that are prevalent in nature, that can be obtained via enzymatic processes and the potential applications of such sucrose derivatives in sugar bioconversion, in particular through the engineering of substrates, enzymes or microorganisms.
Asunto(s)
Polisacáridos/síntesis química , Sacarosa , Estructura Molecular , Plantas/química , Polisacáridos/química , Estereoisomerismo , Sacarosa/análogos & derivados , Sacarosa/síntesis química , Sacarosa/química , Sacarosa/metabolismo , Fosfatos de Azúcar/química , Fosfatos de Azúcar/metabolismoRESUMEN
Red emissive carbon dots from sucrose (SCD) were synthesized using a facile, isolation-free, one-pot method via microwave pyrolysis. Various passivation agents were used along with sucrose, and a relative change in the chemical and optical properties of the carbon dots was investigated. A detailed systematic study of the effect of various passivations, different solvents, pHs, and temperatures on optical properties was carried out. The influence of excitation wavelength and passivation on photoluminescence (PL) is discussed considering the functional groups associated with the passivating agents. The effect of different solvents on dispersibility and PL behavior has been understood in terms of the dielectric properties of the solvents. The decrease in PL intensity of SCD from pH 3 to 11 facilitates pH sensing. The PL of SCD was found to be essentially stable between the temperature range of 20 and 80 °C. Additionally, the effects of physicochemical properties with respect to passivation, such as charge and surface chemistry in determining the cellular uptake and cytotoxicity, are also addressed. Aside from sensors, the potential of SCDs as bioimaging agents has also been studied for mammalian cells. Moreover, SCD exhibits excellent PL stability investigated under different storage conditions for 15 days.
Asunto(s)
Materiales Biocompatibles/química , Carbono/química , Puntos Cuánticos/química , Sacarosa/química , Temperatura , Materiales Biocompatibles/síntesis química , Ensayo de Materiales , Estructura Molecular , Tamaño de la Partícula , Sacarosa/síntesis química , Propiedades de SuperficieRESUMEN
Fatty acid sugar esters are non-ionic surfactant active agents with excellent performance and many uses. This work is devoted to the synthesis of sugar esters by the esterification reaction of sugar with mixed carboxylicpalmitic anhydrides using resin Amberlyst-15 as heterogeneous acid catalyst. These anhydrides should be stable and react as acylating agents. Influence of different reaction parameters, such as the molar ratio (sucrose/anhydride), the type of solvent and the reaction time on the yield of the esterification reaction were studied. The esterification reaction of sucrose with mixed palmitic benzoic anhydride leads to a mixture of sucrose esters of palmitic acid with a good percentage of conversion. The mixed anhydride was both reactive and selective for the preparation of fatty acid ester.
Asunto(s)
Benzoatos/química , Ácidos Carboxílicos/química , Técnicas de Química Sintética/métodos , Ésteres/síntesis química , Ácidos Grasos/síntesis química , Ácido Palmítico/química , Sacarosa/síntesis química , Acilación , Catálisis , Esterificación , Solventes , Estirenos , Tensoactivos/síntesis química , Factores de TiempoRESUMEN
Described are the development of a new synthetic method using ultrasonic irradiation and sodium methoxide as catalyst for a series of pyridinic sucrose esters (py-SEs), derived from transesterification of sucrose with picolinic, nicotinic and isonicotinic methyl esters. The reaction was optimized using a 32 x 2 experimental design, the reaction time, temperature and sucrose: methyl ester molar ratio being evaluated. The method proved to be efficient for obtaining monosubstituted esters (≥83%) with high methyl ester consumption (≥79%). The monosubstituted py-SEs were isolated by semipreparative HPLC, characterized by high-resolution mass spectrometry, calorimetry, vibrational spectroscopy, and 1H and 13C NMR. The py-SEs were tested against E. coli, S. aureos, and P. aeruginosa bacteria with minimum inhibitory concentration values equal or inferior to the reference drugs for both E. coli and P. aeruginosa.
Asunto(s)
Antibacterianos/farmacología , Ésteres/farmacología , Piridinas/farmacología , Sacarosa/farmacología , Antibacterianos/síntesis química , Antibacterianos/química , Escherichia coli/efectos de los fármacos , Ésteres/síntesis química , Ésteres/química , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Pseudomonas aeruginosa/efectos de los fármacos , Piridinas/síntesis química , Piridinas/química , Staphylococcus aureus/efectos de los fármacos , Sacarosa/síntesis química , Sacarosa/químicaRESUMEN
The burst release of active osteogenic factors, which is not beneficial to osteogenesis, is commonly encountered in bone tissue engineering. The aims of this study were to prepare naringin-loaded microsphere/sucrose acetate isobutyrate (Ng-m-SAIB) hybrid depots, reduce the burst release of naringin (Ng), and improve osteogenesis. The morphology and size distributions of electrosprayed Ng-microspheres were characterized by scanning electron microscopy (SEM). The Ng-microspheres and Ng-m-SAIB depots were characterized by Fourier transform infrared spectroscopy (FTIR) and in vitro release studies. In vitro osteoblast-microsphere interactions and in vivo osteogenesis were assessed after implantation of Ng-m-SAIB depots. The addition of sucrose acetate isobutyrate (SAIB) to monodisperse Ng-microspheres did not cause a change in the chemical structure. The performances of the microspheres in osteoblast-microsphere interactions were better when the naringin content was 4% than when it was at 2% and 6%. On the first day following the loading of Ng-microspheres (2%, 4%, and 6%) into SAIB depots, the burst release was reduced dramatically from 70.9% to 6.3%, 73.1% to 7.2%, and 73.9% to 9.9%, respectively. In addition, after 8 weeks, the new bone formation rate in the calvarial defects of SD rats receiving Ng-m-SAIB was 53.1% compared to 21.2% for the control group and 16.1% for the microsphere-SAIB group. These results demonstrated that Ng-m-SAIB hybrid depots may have promise in bone regeneration applications.
Asunto(s)
Modelos Animales de Enfermedad , Flavanonas/administración & dosificación , Microesferas , Osteogénesis/efectos de los fármacos , Cráneo/efectos de los fármacos , Sacarosa/análogos & derivados , Animales , Preparaciones de Acción Retardada/administración & dosificación , Preparaciones de Acción Retardada/síntesis química , Preparaciones de Acción Retardada/metabolismo , Portadores de Fármacos/administración & dosificación , Portadores de Fármacos/síntesis química , Portadores de Fármacos/metabolismo , Flavanonas/síntesis química , Flavanonas/metabolismo , Masculino , Ratones , Osteogénesis/fisiología , Ratas , Ratas Sprague-Dawley , Cráneo/metabolismo , Cráneo/patología , Sacarosa/administración & dosificación , Sacarosa/síntesis química , Sacarosa/metabolismoRESUMEN
1',2,3,3',4,4'-Hexa-O-benzyl-sucrose was converted in good yields into the macrocyclic receptors containing two and three nitrogen atoms in the ring. Their complexation properties towards the ammonium cation were significantly higher than for receptors without any nitrogen atoms in the ring.
Asunto(s)
Éteres Corona/síntesis química , Compuestos de Amonio Cuaternario/química , Sacarosa/síntesis química , Derivados del Benceno/síntesis química , Derivados del Benceno/química , Cromatografía Liquida , Compuestos Corona/síntesis química , Compuestos Corona/química , Éteres Corona/química , Espectroscopía de Resonancia Magnética , Modelos Químicos , Estructura Molecular , Potasio/química , Espectrometría de Masa por Ionización de Electrospray , Sacarosa/análogos & derivados , Sacarosa/químicaRESUMEN
Regioselective formation of 6-O-acylsucroses and 6,3'-di-O-acylsucroses in one pot with good yields was achieved for the first time by a typical acylation method of sucrose via its dibutylstannylene acetal. Pure monoesters at OH-6 and diesters at OH-6,3' obtained by these procedures were readily isolated by simple column chromatography, thus overcoming the main difficulties associated with regioselectivity, efficiency, and isolation techniques for the practical preparation. Explanations for the regioselectivities observed during this stannylene acetal-mediated reaction were also proposed based on the structures of the stannylene acetal in solution and the intramolecular migration of stannylenes.
Asunto(s)
Acetales/química , Sacarosa/análogos & derivados , Sacarosa/química , Sacarosa/síntesis química , Carbohidratos/química , Química/métodos , Cromatografía/métodos , Cromatografía Líquida de Alta Presión/métodos , Ésteres , Espectroscopía de Resonancia Magnética , Modelos Químicos , Compuestos Orgánicos de Estaño/químicaRESUMEN
The preparation of 6,6,1',1',6',6'-hexadeutero sucrose is reported. The synthesis is based on a triple oxidation of a protected sucrose 6,1',6'-triol to the corresponding 6,1',6'-tricarboxylic acid or ester, followed by reduction with lithium aluminium deuteride. This triple oxidation could be achieved either using cat. TEMPO-NaOCl (to the acid) or PDC-Ac(2)O-t-BuOH (to the t-butyl carboxylic ester).
Asunto(s)
Óxidos N-Cíclicos/química , Oxígeno/química , Sacarosa/química , Ácidos Urónicos/química , Aluminio/química , Conformación de Carbohidratos , Secuencia de Carbohidratos , Cromatografía Líquida de Alta Presión , Cromatografía en Capa Delgada/métodos , Deuterio/química , Ésteres , Litio/química , Modelos Químicos , Datos de Secuencia Molecular , Sacarosa/síntesis química , Ácidos Tricarboxílicos/químicaRESUMEN
Novel unsaturated ethers were synthesised in good yields starting from sucrose,using a two-step mild and efficient procedure based on the Gassman method, which consists in forming a vinyl group by the elimination of ethanol from mixed acetals with trimethylsilyl trifluoromethanesulfonate in the presence of alkyl amines. Mixed acetals are readily obtained from the corresponding alcohols and ethyl vinyl ether, using an acidic catalyst. Conventional etherification involving a primary halide was also examined. The monomers thus obtained were successfully polymerised by a free radical mechanism,yielding unbranched linear and soluble polymers with pending sucrose moieties, and some of their physical properties were determined.
Asunto(s)
Éteres/síntesis química , Sacarosa/síntesis química , Éteres/química , Soluciones , Sacarosa/química , Tolueno/químicaRESUMEN
A pyridyl triazole (pyta) modified sucrose ligand was prepared in a seven step synthesis using d-glucose as the protection group for d-fructose and starting from commercially available sucrose. After complexation with Ru(bpy)2Cl2 precursor, the sucrose-conjugated Ru complex of the general formula [Ru(bpy)2(L)]Cl2 was formed. Acidic cleavage of the d-glucose unit led to the first d-fructose conjugated metal complex viad-fructose C6 in literature. Additionally, pyta-modified d-fructose via C1 and the corresponding Ru complex were synthesized. All compounds were analyzed by Rf values, specific rotation, NMR, IR, UV/Vis and fluorescence spectroscopy, mass spectrometry and elemental analysis.
Asunto(s)
2,2'-Dipiridil/análogos & derivados , Fructosa/química , Compuestos Organometálicos/química , Sacarosa/química , Sacarosa/síntesis química , 2,2'-Dipiridil/química , Alquinos/química , Azidas/química , Transporte Biológico , Catálisis , Química Clic , Cobre/química , Humanos , Ligandos , Células MCF-7 , Sacarosa/farmacología , Triazoles/químicaRESUMEN
In the present study, we have coupled detailed acceptor and donor substrate studies of the fructosyltransferase (FTF, levansucrase) (EC 2.4.1.162) from Bacillus subtilis NCIMB 11871, with a structural model of the substrate enzyme complex in order to investigate in detail the roles of the active site amino acids in the catalytic action of the enzyme and the scope and limitation of substrates. Therefore we have isolated the ftf gene, expressed in Escherichia coli, yielding a levansucrase. Consequently, detailed acceptor property effects in the fructosylation by systematic variation of glycoside acceptors with respect to the positions (2, 3, 4 and 6) of the hydroxyl groups from equatorial to axial have been studied for preparative scale production of new oligosaccharides. Such investigations provided mechanistic insights of the FTF reaction. The configuration and the presence of the C-2 and C-3 hydroxyl groups of the glucopyranoside derivatives either as substrates or acceptors have been identified to be rate limiting for the trans-fructosylation process. The rates are rationalized on the basis of the coordination of d-glycopyranoside residues in (4)C(1) conformation with a network of amino acids by Arg360, Tyr411, Glu342, Trp85, Asp247 and Arg246 stabilization of both acceptors and substrates. In addition we also describe the first FTF reaction, which catalyzes the beta-(1-->2)-fructosyl transfer to 2-OH of L-sugars (L-glucose, L-rhamnose, L-galactose, L-fucose, L-xylose) presumably in a (1)C(4) conformation. In those conformations, the L-glycopyranosides are stabilized by the same hydrogen network. Structures of the acceptor products were determined by NMR and mass spectrometry analysis.
Asunto(s)
Bacillus subtilis/enzimología , Dominio Catalítico/fisiología , Hexosiltransferasas/fisiología , Sacarosa/análogos & derivados , Sacarosa/síntesis química , Conformación de Carbohidratos , Catálisis , Estructura Molecular , Oligosacáridos/biosíntesis , Relación Estructura-Actividad , Especificidad por SustratoRESUMEN
We report here a range of new sucrose derivatives obtained from '3-ketosucrose' in aqueous medium with few reaction steps. As an intermediate, 3-amino-3-deoxy-alpha-D-allopyranosyl beta-D-fructofuranoside (1) was obtained via the classical route of reductive amination with much improved yield and high stereoselectivity. Building blocks for polymerization were synthesized by introduction of acrylic-type side chains, for example, with methacrylic anhydride. Corresponding polymers were synthesized. Aminoacyl and peptide conjugates were obtained through conventional peptide synthesis with activated and protected amino acids. Deprotection yielded new glycoderivatives having an unconventional substitution pattern, namely 3-(aminoacylamino) allosaccharides. Both mono- and di-peptide conjugates of allosucrose have been synthesized.
Asunto(s)
Aminoácidos/química , Disacáridos/química , Polimetil Metacrilato/química , Sacarosa/síntesis química , Edulcorantes/síntesis química , Aminación , Conformación de Carbohidratos , Secuencia de Carbohidratos , Isomerismo , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Sacarosa/análogos & derivados , Sacarosa/química , Edulcorantes/químicaRESUMEN
Metabolic labeling based on the click chemistry between alkynyl and azido groups offers a powerful tool to study the function of carbohydrates in living systems, including plants. Herein, we describe the chemical synthesis of six alkynyl-modified sugars designed as analogs to D-glucose, D-mannose, L-rhamnose and sucrose present in plant cell walls. Among these new alkynyl probes, four of them are the 6-deoxy-alkynyl analogs of the corresponding sugars and do not possess any 6-OH groups. The other two are based on a new structural design, in which an ethynyl group is incorporated at the C-6 position of the sugar and the 6-OH group remains. The synthetic routes for both types of probes share common aldehyde intermediates, which are derived from the corresponding 6-OH precursor with other hydroxy groups protected. The overall synthesis sequence of these probes is efficient, concise, and scalable.
Asunto(s)
Desoxiglucosa/análogos & derivados , Monosacáridos/síntesis química , Sacarosa/análogos & derivados , Metabolismo de los Hidratos de Carbono , Química Clic , Glucosa/análogos & derivados , Manosa/análogos & derivados , Estructura Molecular , Monosacáridos/química , Ramnosa/análogos & derivados , Sacarosa/síntesis químicaRESUMEN
Sucrose octa(N-ethyl)carbamate was synthesized directly from sucrose and ethyl isocyanate, and its structure was confirmed by various analytical methods, such as (1)H and (13)C NMR, FTIR, m.p., MS, and optical rotation. Its antibacterial, antifungal and cytotoxic activities were investigated. It exhibited strong inhibition against all bacteria tested, namely S. aureus (MIC 0.18±0.006), B. cereus (MIC 0.094±0.000), M. flavus (MIC 0.28±0.01), L. monocytogenes (MIC 0.18±0.006), P. aeruginosa (MIC 0.094±0.002), S. typhimurium (MIC 0.094±0.002), E. coli (MIC 0.18±0.006) and E. cloacae (MIC 0.18±0.006) and strong antifungal activity towards T. viride (MIC 0.09 ± 0.006), A. versicolor (MIC 0.18 ± 0.01), A. ochraceus (MIC 0.375 ± 0.01) and P. ochrochloron (MIC 0.375 ± 0.04). Furthermore, it showed moderate antitumor potential against human breast (GI50 357.20±14.12), colon (GI50 332.43±11.19) and cervical (GI50 282.67±3.97) cell lines and, more important, without hepatotoxicity.
Asunto(s)
Antibacterianos/farmacología , Antifúngicos/farmacología , Antineoplásicos/farmacología , Carbamatos/farmacología , Sacarosa/análogos & derivados , Ampicilina/farmacología , Animales , Antibacterianos/síntesis química , Antibacterianos/química , Antifúngicos/síntesis química , Antifúngicos/química , Antineoplásicos/síntesis química , Antineoplásicos/química , Aspergillus/efectos de los fármacos , Carbamatos/síntesis química , Carbamatos/química , Línea Celular Tumoral , Bacterias Gramnegativas/efectos de los fármacos , Humanos , Penicillium/efectos de los fármacos , Sacarosa/síntesis química , Sacarosa/química , Sacarosa/farmacología , Porcinos , Trichoderma/efectos de los fármacosRESUMEN
The exo-fructosyltransferase produced from B. subtilis NCIMB 11871 strain transfers the fructose moiety from donor alpha12 linked saccharides such as sucrose, raffinose and stachyose to the acceptor d-galactose, leading to the sucrose analogue, galactosyl-fructoside. Here, we report detailed kinetic studies. The enzyme showed a remarkably high optimal temperature at 50 degrees C and was effectively immobilised on Eupergit C 250 L and Trisopor-Amino. This is also the first report about the equilibrium of the transfructosylation reaction, its activation energy determination, the structure of the product and its preparative scale isolation.
Asunto(s)
Bacillus subtilis/enzimología , Fructosa/química , Glucosa/química , Hexosiltransferasas/química , Sacarosa/análogos & derivados , Sacarosa/síntesis química , Bacillus subtilis/clasificación , Activación Enzimática , Estabilidad de Enzimas , Enzimas Inmovilizadas/química , Especificidad de la Especie , TemperaturaRESUMEN
Sugars produced from photosynthesis in leaves are transported through the phloem tissues within veins and delivered to non-photosynthetic organs, such as roots, stems, flowers, and seeds, to support their growth and/or storage of carbohydrates. However, because the phloem is located internally within the veins, it is difficult to access and to study the dynamics of sugar transport. Radioactive tracers have been extensively used to study vascular transport in plants and have provided great insights into transport dynamics. To better study sucrose partitioning in vivo, a novel radioactive analog of sucrose was synthesized through a completely chemical synthesis route by substituting fluorine-18 (half-life 110 min) at the 6' position to generate 6'-deoxy-6'[(18)F]fluorosucrose ((18)FS). This radiotracer was then used to compare sucrose transport between wild-type maize plants and mutant plants lacking the Sucrose transporter1 (Sut1) gene, which has been shown to function in sucrose phloem loading. Our results demonstrate that (18)FS is transported in vivo, with the wild-type plants showing a greater rate of transport down the leaf blade than the sut1 mutant plants. A similar transport pattern was also observed for universally labeled [U-(14)C]sucrose ([U-(14)C]suc). Our findings support the proposed sucrose phloem loading function of the Sut1 gene in maize, and additionally demonstrate that the (18)FS analog is a valuable, new tool that offers imaging advantages over [U-(14)C]suc for studying phloem transport in plants.
Asunto(s)
Radioisótopos de Flúor , Hidrocarburos Fluorados , Marcaje Isotópico , Hojas de la Planta/metabolismo , Sacarosa/análogos & derivados , Zea mays/metabolismo , Transporte Biológico Activo/fisiología , Radioisótopos de Flúor/farmacocinética , Radioisótopos de Flúor/farmacología , Hidrocarburos Fluorados/síntesis química , Hidrocarburos Fluorados/química , Hidrocarburos Fluorados/farmacocinética , Hidrocarburos Fluorados/farmacología , Proteínas de Transporte de Monosacáridos/metabolismo , Proteínas de Plantas/metabolismo , Sacarosa/síntesis química , Sacarosa/química , Sacarosa/farmacocinética , Sacarosa/farmacologíaRESUMEN
A library of 1-(1',2,3,3',4,4',6-hepta-O-acetyl-6'-deoxy-sucros-6'-yl)-1,2,3-triazoles have been investigated for their antibacterial, antifungal and cytotoxic activities. Most of the target compounds showed good inhibitory activity against a variety of clinically and food contaminant important microbial pathogens. In particular, 1-(1',2,3,3',4,4',6-hepta-O-acetyl-6'-deoxy-sucros-6'-yl)-4-(4-pentylphenyl)-1,2,3-triazole (5) was highly active against all the tested bacteria with minimal inhibitory concentrations (MICs) ranging between 1.1 and 4.4 µM and bactericidal concentrations (MBCs) from 2.2 and 8.4 µM. The compound 1-(1',2,3,3',4,4',6-hepta-O-acetyl-6'-deoxy-sucros-6'-yl)-4-(4-bromophenyl)-1,2,3-triazole (3) showed antifungal activity with MICs from 0.6 to 4.8 µM and minimal fungicidal concentrations (MFCs) ranging between 1.2 and 8.9 µM. Furthermore, some of the compounds possessed moderate cytotoxicity against human breast, lung, cervical and hepatocellular carcinoma cell lines, without showing toxicity for non-tumor liver cells. The above mentioned derivatives represent promising leads for the development of new generation of sugar-triazole antifungal agents.
Asunto(s)
Antibacterianos/farmacología , Antifúngicos/farmacología , Antineoplásicos/farmacología , Sacarosa/farmacología , Triazoles/farmacología , Antibacterianos/síntesis química , Antifúngicos/síntesis química , Antineoplásicos/síntesis química , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Femenino , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Gramnegativas/crecimiento & desarrollo , Bacterias Grampositivas/efectos de los fármacos , Bacterias Grampositivas/crecimiento & desarrollo , Hepatocitos/citología , Hepatocitos/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , Hongos Mitospóricos/efectos de los fármacos , Hongos Mitospóricos/crecimiento & desarrollo , Especificidad de Órganos , Cultivo Primario de Células , Especificidad de la Especie , Relación Estructura-Actividad , Sacarosa/análogos & derivados , Sacarosa/síntesis química , Triazoles/síntesis químicaRESUMEN
Thermolysin catalyses the formation of sucrose esters from sucrose and vinyl laurate in dimethylsulfoxide, with a specific activity of 53 nmol/min/mg and 2-O-lauroyl-sucrose as the main product. Such transesterification reactions are normally observed only when the mechanism involves an acyl enzyme intermediate, as with lipases or serine proteases, and not with metalloproteases like thermolysin. A possible reason is the affinity of the active site of thermolysin for sugar moieties, as for the potent inhibitor phosphoramidon. The reaction is not catalysed by other proteins under the same conditions, and is inhibited by removal of the active site zinc.
Asunto(s)
Dimetilsulfóxido/química , Sacarosa/análogos & derivados , Sacarosa/química , Termolisina/química , Carboxipeptidasas/química , Carboxipeptidasas A , Catálisis , Cromatografía Líquida de Alta Presión , Cromatografía en Capa Delgada , Dimetilformamida/química , Enzimas Inmovilizadas/química , Esterificación , Ésteres/química , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Muramidasa/química , Albúmina Sérica Bovina/química , Solubilidad , Sacarosa/síntesis químicaRESUMEN
Nitrosourea derivatives of sucrose have been synthesized for the purpose of obtaining anticancer agents with activity against brain cancer. Two such compounds, 6,6'-dideoxy-6,6'-di(3-methyl-3-nitrosoureido) sucrose (13) and 1', 6,6'-trideoxy-1',6,6-tri(3-methyl-3-nitrosoureido) sucrose (14), and their respective acetylated derivatives 15 and 16 have been prepared from sucrose. Compounds 13 and 14 have demonstrated antitumor activity against both L1210 leukemia and ependymoblastoma brain tumor in mice.