Mouse monoclonal antibodies to bovine blood group antigens: additional results.

Seven fusions of mouse myeloma cells with spleen cells from mice immunized with bovine red cells yielded 61 clones producing discriminant antibodies out of total of 651 secreting clones. Although antigenic factors of all known bovine blood group systems were present on the donors' cells, the antibodies identified reacted with antigenic factors from only five systems, A, B, F, S and Z. The antibody specificities produced by more than two clones were anti-A1 or -A2 (21 clones), -S (9),- Z(6),-G' (3) and -V1 (3). The absence of clones secreting antibodies to antigens of the other systems, especially the complex C system, remains unexplained. The properties of the antibodies reacting with antigens of the S system (anti-SU", anti-SUU') and of the B system (O-like antibodies) are in accordance with previous interpretations of polyclonal sera and with present knowledge of the genetic map of the B system.

The C system of cattle blood groups. 1. Additional factors in the system.

Four additional cattle blood group antigenic factors, provisionally termed F1, F6, F10 and F15, were shown to belong to the C system. Factor F1 appears to be a linear subtype of C" (initially designated F2, or P1B1). It is suggested that future international nomenclature should adopt C"1 and C"2 in place of F1 and C". No phenogroup was found to include C" together with C2 or C1, but a few phenogroups lack the three factors. Thus C1, C2 and C" do not form a closed system within the C system as concluded by Duniec et al. (1973). The effectiveness of the additional factors to uncover the genetic variability of the C system, and to translate phenotypes into genotypes is exemplified in the Charolais breed.