Assuntos
Febre por Flebótomos/diagnóstico , Febre por Flebótomos/virologia , Phlebovirus/genética , Pré-Escolar , Febre/etiologia , Variação Genética/genética , Grécia , Humanos , Masculino , Febre por Flebótomos/complicações , RNA Viral/genética , Convulsões Febris/etiologia , Homologia de Sequência do Ácido NucleicoRESUMO
Phleboviruses, among them Toscana virus (TOSV), are endemic in the Mediterranean countries. In the present study sera collected from 219 apparently healthy individuals residents of seven islands in the Aegean Sea, Greece, were tested for the detection of TOSV IgG antibodies. Overall, TOSV IgG antibodies were detected in 46/219 (21%) individuals. Samos and Evia islands presented the highest seroprevalence (40% and 34.78%, respectively). There was no significant difference among males and females, while increased age was significantly associated with seropositivity. TOSV, and phleboviruses in general, have to be included in the differential diagnosis of febrile or acute neurological cases among residents or travelers in Mediterranean countries during the summer time, especially when sand fly bites are reported.
Assuntos
Febre por Flebótomos/epidemiologia , Vírus da Febre do Flebótomo Napolitano/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antivirais/sangue , Distribuição de Qui-Quadrado , Criança , Feminino , Grécia/epidemiologia , Humanos , Imunoglobulina G/sangue , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Febre por Flebótomos/imunologia , Febre por Flebótomos/virologia , Vírus da Febre do Flebótomo Napolitano/imunologia , Estudos SoroepidemiológicosRESUMO
In June 2004 an 8-year-old boy was admitted to a hospital in Thessaloniki, Greece, because of high fever, tachypnea, hypotonia, diarrhea, and tonoclonic convulsions. Phlebovirus infection was diagnosed by IgG seroconversion to Toscana virus. As IgM antibodies were not detected, it is suggested that this was an acute infection caused by a phlebovirus virus distinct from Toscana virus. Complication by a hospital-acquired Pseudomonas aeruginosa pneumonia resulted in 2 months of hospitalization. Slight ataxia was still present on discharge.
Assuntos
Infecções por Bunyaviridae/diagnóstico , Meningoencefalite/diagnóstico , Phlebovirus/isolamento & purificação , Pneumonia Bacteriana/complicações , Infecções por Pseudomonas/complicações , Pseudomonas aeruginosa , Aciclovir/uso terapêutico , Antibacterianos/uso terapêutico , Antivirais/uso terapêutico , Infecções por Bunyaviridae/complicações , Ceftriaxona/uso terapêutico , Criança , Colistina/uso terapêutico , Infecção Hospitalar/complicações , Infecção Hospitalar/microbiologia , Quimioterapia Combinada , Ensaio de Imunoadsorção Enzimática , Grécia , Humanos , Imunoglobulina G/sangue , Masculino , Meningoencefalite/complicações , Meningoencefalite/tratamento farmacológico , Meningoencefalite/virologia , Phlebovirus/classificação , Phlebovirus/imunologia , Pneumonia Bacteriana/diagnóstico , Pneumonia Bacteriana/tratamento farmacológico , Infecções por Pseudomonas/etiologia , Resultado do TratamentoRESUMO
A field study on the distribution of phlebotomine sandflies was carried out during summer months of 2009 and 2010 in eight sites in two Ionian islands and in northern Greece. A total of 490 sandflies (74.5% females) were collected. Six species of the Phlebotomus genus and two of the Sergentomyia genus were identified. The species with the widest distribution in the islands were Phlebotomus neglectus (32.8%), Phlebotomus similis (30.3%), Phlebotomus tobbi (16.7%), and P. perfiliewi (15.9%), whereas P. simici (50%), P. neglectus (24.5%), and P. tobbi (9.6%) predominated in the mainland. As most of these species are proven or suspected vectors of human and animal pathogens, prevention measures have to be taken in these areas during the summer months when sandflies are active.
Assuntos
Insetos Vetores/fisiologia , Psychodidae/fisiologia , Animais , Dípteros/fisiologia , Feminino , Geografia , Grécia , Masculino , Phlebotomus/fisiologia , Densidade Demográfica , Psychodidae/classificação , Distribuição por SexoRESUMO
The evolutionary patterns of each genome RNA segment of Crimean-Congo Hemorrhagic Fever virus (CCHFV) were investigated. Nucleotide sequences from 40 CCHFV strains isolated during time period spanning from 1956 to 2003 were tested. It was found that there is not any global molecular clock ticking at the same rate for CCHFV strains; however a local clock is applicable in S RNA segment among strains in the individual clades. African strains seem to evolve faster than strains from other geographic regions. In the comparative study of 18 strains it was found that the evolution rates of S, M and L segments are 0.34 x 10(-4), 1.22 x 10(-4), and 1.01 x 10(-4), respectively. Many discrepancies in clustering were seen, most probably caused by reassortment events, leading in complex patterns of evolution.