Classifiers based on artificial intelligence in the prediction of recently planted coffee cultivars using a Remotely Piloted Aircraft System.

The classification and prediction methods through artificial intelligence algorithms are applied in different sectors to assist and promote intelligent decision-making. In this sense, due to the great importance in the cultivation, consumption and export of coffee in Brazil and the technological application of the Remotely Piloted Aircraft System (RPAS) this study aimed to compare and select models based on different data classification techniques by different classification algorithms for the prediction of different coffee cultivars (Coffea arabica L.) recently planted. The attributes evaluated were height, crown diameter, total chlorophyll content, chlorophyll A and chlorophyll B, Foliar Area Index (LAI) and vegetation indexes NDVI, NDRE, MCARI1, GVI, and CI in six months. The data were prepared programming language Python using algorithms of Decision Trees, Random Forest, Support Vector Machine and Neural Networks. It was evaluated through cross-validation in all methods, the distribution by FreeViz, the hit rate, sensitivity, specificity, F1 score, and area under the ROC curve and percentage and predictive performance difference. All algorithms showed good hits and predictions for coffee cultivars (0.768% Decision Tree, 0.836% Random Forest, 0.886 Support Vector Machine and 0.899 Neural Networks) and the Neural Networks algorithm produced more accurate predictions than other tested algorithm models, with a higher percentage of hits for the classes considered.

Complications associated with submental liposuction: a scoping review.

BACKGROUND: Liposuction is one of the most commonly performed cosmetic procedures worldwide. Complications associated with submental liposuction are rare. However, when they occur they are significant and can cause disfiguring consequences. The objective of this study was evaluated complications from submentual liposuction in literature and description of clinical experience of complication after submentual liposuction. MATERIAL AND METHODS: At first, a scoping review was carried out online search with no time restrictions for complications after submental liposuction was performed in the databases Medline / PubMed, Embase, and Web of Science. The variables analyzed were: age, sex, type of esthetic procedure, anesthesia, complications, time after Procedure, treatment, follow-up care, and sequelae. Then, a case of a patient with submental hematoma after an aesthetic procedure for submental liposuction was described. RESULTS: Firstly, 539 articles were selected, after application of the inclusion criteria, 4 studies were included. Most cases were female (8:1), with a mean age of 55.77 years. Postoperative complications were found, such as submental depression, submental edema, hypertrophic scar formation, scar contracture, cervical necrotizing fasciitis, Cervico-facial dystonia and transient facial nerve paralysis. The follow-up period for cases ranged from 3 to 12 months. The clinical case presented there was no sequelae. CONCLUSIONS: Submental liposuction requires the surgeon's attention. Anatomical knowledge, correct clinical and surgical management, diagnosis, and immediate approach to adverse situations are points that must be respected in this type of esthetic procedure to avoid more serious complications.

Is the injection of tramadol effective at control of pain after impacted mandibular third molar extractions? A systematic review and meta-analysis.

BACKGROUND: Third molar extraction is among the most common surgical procedures performed by oral-maxillofacial surgeons. Postoperative pain, swelling and trismus are common, especially in wisdom teeth, due to trauma to local tissues and the duration of the surgical procedure, among other factors. MATERIAL AND METHODS: This systematic review was conducted in accordance with the 'Preferred Reporting Items for Systematic Reviews and Meta-Analyses' in order to answer the focused question: 'Is the local submucosal injection of tramadol effective at the control of postoperative pain in patients submitted to impacted mandibular third molar extractions?'. We analyzed papers published until March 30, 2021 in the MEDLINE|PubMed, Web of Science and Cochrane Library databases. Gray literature was also consulted. Standard pairwise meta-analyses of direct comparisons were performed using a fixed-effect model; I2 ≥ 50 % or ≥ 75 % indicated moderate or high heterogeneity, respectively. Risk of bias was assessed by Cochrane Collaboration's tool. RESULTS: In total, 172 participants (98 males and 74 females, aged 18 or over) from three randomized placebo-controlled trials were considered for analysis. The submucosal injection of 2 ml of tramadol adjacent to the impacted mandibular third molar was effective in controlling pain up to 6-hours after surgery, in increasing the onset of consumption of rescue analgesic and in reducing the total number of rescue analgesics used. CONCLUSIONS: The submucosal injection of tramadol can be considered a safe and effective procedure for pain control after impacted mandibular third molar extractions.

Head and neck cancer associated with myiasis.

Head and neck cancer patients represent a risk group for the development of fly larvae infestation in neoplastic wounds. This condition can accelerate the disease progression and increase its lethality due to local or systemic complications. The aim of this study was to conduct a scoping review on head and neck cancer associated with myiasis in order to answer the focal question: what are the occurrence, diagnosis, aetiology, treatment and outcomes of head and neck cancer associated with myiasis? This paper was structured based on the five-steps methodology proposed by Arksey and O'Malley (Int J Soc Res Methodol 2005;1:19-32), and followed the PRISMA Extension for Scoping Reviews (PRISMA-ScR), OSF Registries protocol. The electronic search was performed in the MEDLINE/PubMed, Embase and SciELO.org databases for articles published up to 28 February 2021. In total, 38 articles and 56 patients were included. Most patients were male (66%), and the mean age was 66.63 years. Cases were predominantly associated with squamous or basal cell cancer. The most affected anatomical sites were the eyes, scalp, ears and oral cavity, and the most frequent type of larva was Crisomyia (13%). Manual removal of the larvae was considered the standard treatment, associated or not with antibiotics, analgesics and antiparasitic drugs.

Very early prediction of response to HCV treatment with PEG-IFN-alfa-2a and ribavirin in HIV/HCV-coinfected patients.

The objective of this study was to find very early viral kinetic markers to predict nonresponse to hepatitis C virus (HCV) therapy in a group of human immunodeficiency virus (HIV)/HCV-coinfected patients. Twenty-six patients (15 HCV genotype-1 and 11 genotype-3) were treated with a 48-week regimen of peginterferon-alfa-2a (PEG-IFN) (180 µg/week) and weight-based ribavirin (11 mg/kg/day). Samples were collected at baseline; 4, 8, 12, 18, 24, 30, 36 and 42 h; days 2, 3, 4, 7, 8, 15, 22, 29, 43 and 57 then weekly and monthly. Five patients discontinued treatment. Seven patients (27%) achieved a sustained virological response (SVR). Nadir HCV RNA levels were observed 1.6 ± 0.3 days after initiation of therapy, followed by a 0.3- to 12.9-fold viral rebound until the administration of the second dose of PEG-IFN, which were not associated with SVR or HCV genotype. A viral decline <1.19 log for genotype-1 and <0.97 log for genotype-3, 2 days after starting therapy, had a negative predictive value (NPV) of 100% for SVR. The day 2 virological response had a similar positive predictive value for SVR as a rapid virological response at week 4. In addition, a second-phase viral decline slope (i.e., measured from day 2 to 29) <0.3 log/week had a NPV = 100% for SVR. We conclude that first-phase viral decline at day 2 and second-phase viral decline slope (<0.3 log/week) are excellent predictors of nonresponse. Further studies are needed to validate these viral kinetic parameters as early on-treatment prognosticators of nonresponse in patients with HCV and HIV.

Evidence for multiple sylvatic transmission cycles during the 2016-2017 yellow fever virus outbreak, Brazil.

OBJECTIVES: Since December 2016, Brazil has experienced an unusually large outbreak of yellow fever (YF). Whether urban transmission may contribute to the extent of the outbreak is unclear. The objective of this study was to characterize YF virus (YFV) genomes and to identify spatial patterns to determine the distribution and origin of YF cases in Minas Gerais, Espírito Santo and Rio de Janeiro, the most affected Brazilian states during the current YFV outbreak. METHODS: We characterized near-complete YFV genomes from 14 human cases and two nonhuman primates (NHP), sampled from February to April 2017, retrieved epidemiologic data of cases and used a geographic information system to investigate the geospatial spread of YFV. RESULTS: All YFV strains were closely related. On the basis of signature mutations, we identified two cocirculating YFV clusters. One was restricted to the hinterland of Espírito Santo state, and another formed a coastal cluster encompassing several hundred kilometers. Both clusters comprised strains from humans living in rural areas and NHP. Another NHP lineage clustered in a basal relationship. No signs of adaptation of YFV strains to human hosts were detected. CONCLUSIONS: Our data suggest sylvatic transmission during the current outbreak. Additionally, cocirculation of two distinct YFV clades occurring in humans and NHP suggests the existence of multiple sylvatic transmission cycles. Increased detection of YFV might be facilitated by raised awareness for arbovirus-mediated disease after Zika and chikungunya virus outbreaks. Further surveillance is required, as reemergence of YFV from NHPs might continue and facilitate the appearance of urban transmission cycles.

Biological parameters and fertility life table of Aphis forbesi Weed, 1889 (Hemiptera: Aphididae) on strawberry.

This study provides the first contribution of the biology and life table of Aphis forbesi Weed, 1889 (Hemiptera: Aphididae), an important strawberry pest throughout the world. This species lives in the crown and leaf petioles of the plant. It is difficulty to rear this species in laboratory due to protocooperation with ants observed only in the field. We studied the life cycle of A. forbesi on the leaves of the Albion strawberry cultivar at 25 ± 2 °C, 60 ± 10% relative humidity, and a 12-h photophase. The experiment was randomised with 100 replicates. The parameters of the fertility life table were calculated using TabVida. In the population studied, 25% and 46% had four and three instars, respectively. A mean of 1.43 nymphs per female per day was generated. The mean reproductive period was seven days and the mean longevity was 10 days. In every 11 days there is a generation of A. forbesi, where each female has the potential to generate between 6 to 9 individuals daily, increasing its population by 1.2 times. The average life cycle was 16.8 days. High viability observed in all instars and the resulting values of R0, rm and λ suggest that A. forbesi has the capacity to increase their numbers in a short period of time, while generating high populations in strawberry crops, requiring differential management.

Evaluation of an IgG enzyme-linked immunosorbent assay for dengue diagnosis.

BACKGROUND: The hemagglutination inhibition (HI) test has been one of the standards, with the IgM antibody capture ELISA (MAC-ELISA), for the diagnosis of dengue virus infections. The spread of dengue throughout the world and the increasing number of cases to be tested makes an ELISA-format test for IgG antibodies to replace the HI test highly desirable. OBJECTIVES: Evaluate the use of the IgG-ELISA as a substitute for the HI test in dengue diagnosis. STUDY DESIGN: Paired serum samples defined as being from primary or secondary dengue virus infections by HI, were tested by an ELISA that detects IgG antibodies. The correlations of titers and serologic interpretations between these two tests were examined. RESULTS: The IgG-ELISA showed a low correlation with the HI in primary infections, and a higher correlation in secondary infections because of the influence of IgM antibodies in the HI test. Nevertheless, IgG ELISA titers could be reliably associated with primary or secondary infections when analyzed by days after onset of symptoms, and can be used to characterize the immune response after flavivirus infections. CONCLUSION: The combination of the IgM and IgG ELISAs may be used to serologically diagnose dengue virus infections, since the IgG ELISA can substitute for the HI test in characterizing the immune response to dengue virus infections.

Diagnosis of dengue infection by detecting specific immunoglobulin M antibodies in saliva samples.

To investigate whether saliva could be used for diagnosis of recent dengue, serum and saliva samples were collected simultaneously from patients with suspected dengue infection. Sera (1:10 dilution) and saliva (undiluted) were tested by using an IgM capture enzyme linked immunosorbent assay (MAC-ELISA) with minor modifications (serum and saliva absorption for 3 h at 37 degrees C). The quality of saliva was evaluated by determining the IgG total concentration (enzyme immunoassay) which ranged from 2.7 to > 50 mg/l. Recent dengue infection was confirmed in 38 cases. Forty-six serum and saliva specimens were collected from these patients 1-30 days after the onset of symptoms. IgM was detected in 65.8% saliva samples. High rate of positivity ( > 80%) was observed for the saliva samples collected > or = 5 days after the onset of the disease. Fifty serum and saliva samples from other 32 patients with rash diseases were also tested and all the specimens were unreactive by MAC-ELISA. These results indicate that saliva may be a convenient non-invasive alternative to serum for diagnosis of recent dengue fever infection, especially for epidemiological studies during outbreaks of the disease.

Dengue virus surveillance: the co-circulation of DENV-1, DENV-2 and DENV-3 in the State of Rio de Janeiro, Brazil.

This study presents the results obtained in the monitoring of dengue virus (DENV) transmission in the Greater Metropolitan Region of the State of Rio de Janeiro, in the period 2000-2001. A total of 5324 serum samples from suspected cases of dengue were analysed in order to confirm dengue infection. The introduction of DENV-3 to the region in December 2000 resulted in the co-circulation of three serotypes: DENV-1, DENV-2 and DENV-3. In this study, virus isolation and/or reverse transcriptase PCR (RT-PCR) confirmed 52.3% (42/79) of DENV-3 cases, showing the importance of acute serum samples in the virological surveillance of the disease. Despite the introduction of a new serotype, an outbreak due to DENV-1 was observed in the municipality of Niteroi. The restriction site-specific PCR (RSS-PCR) patterns obtained for DENV-1 and DENV-2 isolated in that period showed that those strains belonged to the subtypes previously circulating in the state. DENV-3 RSS-PCR patterns confirmed that these viruses belonged to subtype C (Sri Lanka/India strains), represented by the strain circulating on the American continent. These data showed the importance of an active surveillance programme in countries where dengue is endemic.

Tissue-typing in human massive allografts of frozen bone.

The cases of twenty-six patients who received a massive allotransplant of frozen bone, with a known degree of histocompatibility between the donor and the recipient, were studied. Twenty-two patients were followed for more than two years (range, twenty-four to ninety-two months). Twenty-three biopsies were performed in sixteen patients from nine to seventy-eight months after transplantation. No clear relationship could be established between the degree of histocompatibility of the donor and the recipient and the incorporation of the graft, probably in part due to the number of variables involved and the polymorphism of the HLA system. However, no early massive resorption of the transplant was seen in this series, in which, by the design of the protocol, no recipients had pre-existing circulating antibodies to the antigens of the donor. Two allografts showed infiltration by round cells and vascular lesions in the absence of infection, which is suggestive of an immune response against antigens from the donor. Both matched poorly with the donor for HLA antigens. The individual who had the strongest reaction was the only recipient in the series who had a massive failure of the transplant.

Aortic reconstruction with crimped bovine pericardial conduits.

BACKGROUND AND AIM OF THE STUDY: A bovine pericardial conduit processed in glutaraldehyde was designed, incorporating the principle of crimping used for synthetic vascular prostheses. The crimping process did not affect the integrity of collagen fibers and tissue structure. This conduit, designed for aortic reconstruction, is available in different sizes, with or without a biological valve. METHODS: Between October 1989 and May 1997, 40 patients with aortic dissection, aortic aneurysm, aortic coarctation or aortoiliac occlusive disease underwent aortic reconstruction using this vascular substitute. Procedures included total reconstruction of the ascending aorta and aortic valve with reimplantation of coronary arteries (nine patients), single ascending thoracic aorta (six), descending thoracic aorta (two), aortic arch (one) and thoracoabdominal aorta (one); the abdominal aorta was reconstructed in 21 cases, including those undergoing aortoiliac or aortofemoral bypass. RESULTS: The hospital mortality rate was 20% (eight patients); causes of deaths were low cardiac output, recurrence of aortic dissection, multiple organ failure and bleeding. Mean follow up was 3.6 years; total follow up was 114 patient-years. Late conduit-related complications occurred in four patients, including a limb obstruction in one patient subjected to aortofemoral bypass and infection of three resulting in pseudoaneurysm (incidence of 3.5 +/- 1.8% per patient-year). All underwent reoperation. There were four late deaths due to sudden death, coronary artery disease, pneumonia and metabolic complications of diabetes and renal failure (incidence of 3.5 +/- 1.8% per patient year). The eight-year actuarial survival rate was 63.7 +/- 11.6%, including hospital mortality, and the eight-year actuarial freedom from conduit failure due to primary tissue structural degeneration was 100%. CONCLUSIONS: The crimping design provides a circular tube which makes construction of the anastomosis easier, retains its shape with bending, and avoids kinking. The material is very soft, easy to handle and suture, coapts nicely to suture lines resulting in a hemostatic anastomosis. The eight-year follow up demonstrated a satisfactory performance without report of fibrosis, calcification or aneurysmal dilation.

A randomized clinical trial with high dose of chloroquine for treatment of Plasmodium falciparum malaria in Brazil.

This clinical trial compared parasitological efficacy, levels of in vivo resistance and side effects of oral chloroquine 25 mg/Kg and 50 mg/Kg in 3 days treatment in Plasmodium falciparum malaria with an extended followed-up of 30 days. The study enrolled 58 patients in the 25 mg/Kg group and 66 in the 50 mg/Kg group. All eligible subjects were over 14 years of age and came from Amazon Basin and Central Brazil during the period of August 1989 to April 1991. The cure rate in the 50 mg/Kg group was 89.4% on day 7 and 71.2% on day 14 compared to 44.8% and 24.1% in the 25 mg/Kg group. 74.1% of the patients in the 25 mg/Kg group and 48.4% of the patients in the 50 mg/Kg group had detectable parasitaemia at the day 30. However, there was a decrease of the geometric mean parasite density in both groups specially in the 50 mg/Kg group. There was 24.1% of RIII and 13.8% of RII in the 25 mg/Kg group. Side effects were found to be minimum in both groups. The present data support that there was a high level resistance to chloroquine in both groups, and the high dose regimen only delayed the development of resistance and its administration should not be recommended as first choice in malaria P. falciparum therapy in Brazil.

Dengue infection in Paracambi, State of Rio de Janeiro, 1990-1995.

A seroepidemiological survey was carried out during 1994 in the municipality of Paracambi, state of Rio de Janeiro. Haemagglutination inhibition test positivity was detected in 145 out of 370 (39.2%) schoolchildren. The frequency of positive test by sex was 53.8% (78/145) female and 46.2% (67/145) male. Distribution by age showed the increasing of antibody positivity in older children. Strains of dengue virus type 1 and dengue virus type 2 were isolated before (1990) showing the co-circulation of both serotypes in that area. The house index infestation of Aedes albopictus and Aedes aegypti has been determined.

PIP: Dengue virus type 1 (DEN-1) was first isolated in Rio de Janeiro, Brazil, in 1986. In 1990, another serotype, DEN-2, was introduced. Since then, both strains have co-circulated in the state, especially during the rainy season. This study assessed the prevalence of dengue virus infection in Paracambi--a periurban municipality of Rio de Janeiro with environmental conditions that allow natural breeding of Aedes aegypti and A. albopictus. In 1990-91, 1209 dengue cases were reported in the municipality and laboratory studies confirmed 21 cases of dengue hemorrhagic fever. Serologic samples collected in 1994 from 370 randomly selected schoolchildren 4-16 years of age revealed hemagglutination inhibition test antibody titers equal to or greater than 1/20 to DEN-1 and/or DEN-2 in 145 children (39.2%). The geometric averages of antibody titers were 1/75 for DEN-1 and 1/30 for DEN-2. Entomological surveillance revealed declines in the house index of A. aegypti to 2.0% or under by 1995, while the index of A. albopictus remained at 0.6-7.4%. The decline in A. aegypti was accompanied by a dramatic reduction in dengue virus cases in 1991-94. However, as a result of the interruption of vectors measures control in 1994, the number of dengue cases has shown an upward trend since 1995.

Stability of XIST repression in relation to genomic imprinting following global genome demethylation in a human cell line.

DNA methylation is essential in X chromosome inactivation and genomic imprinting, maintaining repression of XIST in the active X chromosome and monoallelic repression of imprinted genes. Disruption of the DNA methyltransferase genes DNMT1 and DNMT3B in the HCT116 cell line (DKO cells) leads to global DNA hypomethylation and biallelic expression of the imprinted gene IGF2 but does not lead to reactivation of XIST expression, suggesting that XIST repression is due to a more stable epigenetic mark than imprinting. To test this hypothesis, we induced acute hypomethylation in HCT116 cells by 5-aza-2'-deoxycytidine (5-aza-CdR) treatment (HCT116-5-aza-CdR) and compared that to DKO cells, evaluating DNA methylation by microarray and monitoring the expression of XIST and imprinted genes IGF2, H19, and PEG10. Whereas imprinted genes showed biallelic expression in HCT116-5-aza-CdR and DKO cells, the XIST locus was hypomethylated and weakly expressed only under acute hypomethylation conditions, indicating the importance of XIST repression in the active X to cell survival. Given that DNMT3A is the only active DNMT in DKO cells, it may be responsible for ensuring the repression of XIST in those cells. Taken together, our data suggest that XIST repression is more tightly controlled than genomic imprinting and, at least in part, is due to DNMT3A.

Aphid Species and Population Dynamics Associated with Strawberry.

Aphids are among the major pests associated with strawberries in Southern Brasil. In this study, we identified the main species that occur in strawberry fields in the states of Paraná and Rio Grande do Sul, Brasil. We also compared the effectiveness of different sampling methods and studied the population dynamics of aphid species during two strawberry crop cycles in the municipality of Pinhais, state of Paraná, Brasil. Chaetosiphon fragaefolii (Cockerell) and Aphis forbesi Weed were the main species associated with strawberry. The method of hit plant and the Möericke trap showed equal effectiveness to capture wingless and winged insects. The peak population of aphids in the state of Paraná occurred from September to November. This information can help producers to implement strategies to monitor and control the major aphid species that occur in strawberry culture.

New insights into the complex mixture of latex cysteine peptidases in Calotropis procera.

The latex of Calotropis procera is a rich source of proteolytic activity. This latex is known to contain two distinct cysteine peptidases: procerain and procerain B. In this study, new cysteine peptidases were purified from C. procera latex. The enzymes were purified by two sequential ion-exchange chromatography steps (CM-Sepharose plus Resource S(®)) at pH 5.0 and 6.0. The purified enzymes had molecular mass spectra corresponding to CpCP-1=26,213, CpCP-2=26,133 and CpCP-3=25,086 Da. These enzymes exhibited discrete differences in terms of enzymatic activity at a broad range of pH and temperature conditions and contained identical N-terminal amino acid sequences. In these respects, these three new proteins are distinct from those previously studied (procerain and procerain B). Circular dichroism analysis revealed that the new peptidases contain extensive secondary structures, α(15-20%) and ß(26-30%), that were stabilized by disulfide bonds. The purified enzymes exhibited plasma-clotting activity mediated by a thrombin-like mechanism. The set of results suggest the three isolated polypeptides correspond to different post-translationally processed forms of the same protein.

Biological parameters and fertility life table of Aphis forbesi Weed, 1889 (Hemiptera: Aphididae) on strawberry / Parâmetros biológicos e tabela de vida de fertilidade de Aphis forbesi Weed, 1889 (Hemiptera: Aphididae) em morangueiro

Abstract This study provides the first contribution of the biology and life table of Aphis forbesi Weed, 1889 (Hemiptera: Aphididae), an important strawberry pest throughout the world. This species lives in the crown and leaf petioles of the plant. It is difficulty to rear this species in laboratory due to protocooperation with ants observed only in the field. We studied the life cycle of A. forbesi on the leaves of the Albion strawberry cultivar at 25 ± 2 °C, 60 ± 10% relative humidity, and a 12-h photophase. The experiment was randomised with 100 replicates. The parameters of the fertility life table were calculated using TabVida. In the population studied, 25% and 46% had four and three instars, respectively. A mean of 1.43 nymphs per female per day was generated. The mean reproductive period was seven days and the mean longevity was 10 days. In every 11 days there is a generation of A. forbesi, where each female has the potential to generate between 6 to 9 individuals daily, increasing its population by 1.2 times. The average life cycle was 16.8 days. High viability observed in all instars and the resulting values of R0, rm and λ suggest that A. forbesi has the capacity to increase their numbers in a short period of time, while generating high populations in strawberry crops, requiring differential management.

Resumo Este trabalho apresenta a primeira contribuição ao estudo de biologia e tabela de vida de fertilidade de Aphis forbesi, Weed, 1889 (Hemiptera: Aphididae), uma importante praga de morangueiro no mundo. Esta espécie se desenvolve na coroa e pecíolo do morangueiro. O desenvolvimento desta espécie em laboratório apresentou dificuldades, possivelmente devido à protocooperação com formigas, observada em campo durante coletas. O ciclo de vida de A. forbesi foi estudado em folhas de morangueiro cultivar ‘Albion’ a 25 ± 2 °C, 60 ± 10% umidade relativa, e fotofase de 12 horas. O experimento foi inteiramente casualizado com 100 repetições. Os parâmetros da tabela de vida de fertilidade foram calculados usando o software TabVida. Na população estudada observou-se que as ninfas apresentaram três e quatro instares, sendo 46 e 25% respectivamente, dos indivíduos que completaram o ciclo de vida. Foi gerada uma média de 1,43 ninfas/ fêmea/ dia. O período reprodutivo médio foi de 7 dias e a longevidade média 10 dias. A cada 11 dias ocorre uma geração de A. forbesi onde cada fêmea tem capacidade de gerar de 6 a 9 indivíduos aumentando em 1,2 vezes a população. O ciclo de vida de A. forbesi durou em média 16,8 dias. A alta viabilidade observada em todos os estádios, e os valores de R0, rm e λ sugerem que A. forbesi tem a capacidade de aumentar seu número em um curto período de tempo, gerando altas populações no cultivo do morangueiro, exigindo manejo diferenciado.