RESUMO
AIM: To investigate the effects of liver fibrosis (LF) on the pro-inflammatory mediators and periapical bone resorption of apical periodontitis (AP) in rats. METHODOLOGY: Forty male Wistar rats were distributed into four groups: C - control, AP - rats with AP, LF - rats with LF, AP + LF - rats with AP and LF. LF was induced by carbon tetrachloride administration for 8 weeks and surgical bile duct ligation for 4 weeks; AP was induced in the teeth of rats by dental pulp exposure to the oral environment for 30 days. Jaws and livers were removed after euthanasia. Haematoxylin and Eosin (H&E) and Picrosirius Red (PSR) staining were used to confirm fibrosis in the livers. The jaws were analysed using H&E staining, immunohistochemical assays of interleukin (IL)-1ß, IL-6 and tumour necrosis factor-alpha (TNF-α). Student's t-test and Mann-Whitney's U-test were used for statistical analysis (P < 0.05). RESULTS: Inflammatory infiltrate was moderate in the AP group and severe in the AP + LF group (P < 0.05). Periapical bone resorption was significantly larger in the AP + LF group compared with the AP group (P < 0.05). IL-1ß, IL-6 and TNF-α levels were significantly higher in AP + LF group when compared to the AP group (P < 0.05). CONCLUSION: More intense inflammatory infiltrate, greater amounts of pro-inflammatory cytokines and increased periapical bone resorption were observed in the presence of liver fibrosis in rats with exposed pulps.
Assuntos
Periodontite Periapical , Animais , Citocinas , Cirrose Hepática , Masculino , Periodontite Periapical/complicações , Ratos , Ratos Wistar , Fator de Necrose Tumoral alfaRESUMO
SUMMARY: T cells are coordinators of the immune response and have been shown to play a central role in the pathophysiology of asthma. A good understanding of the T cells functions in asthma is important for therapeutic reasons, in particular for the choice of biological treatments in severe asthma. Although classically considered a Th2 disease, it is now clear that other types of T cells contribute for the pathophysiology and the heterogeneity of asthma. We here review how the different subsets of T cells are involved in the different phenotypes/endotypes of asthma and how this may influence the treatment of the disease.
Assuntos
Asma , Células Th2 , Asma/tratamento farmacológico , Humanos , Fenótipo , Subpopulações de Linfócitos T/metabolismoRESUMO
OBJECTIVE: The main objective of the present study was to investigate the possible osteostimulatory action of recombinant human matrix metalloproteinase-2 (rhMMP-2) implanted in a bone defect made in calvaria of rats, bounded to the monoolein as carrier. METHODS: Forty-four adult male Wistar rats (about 600 g body weight) underwent surgery in order to create a spherical defect in parietal bone on the right side of the median sagittal suture by using 4 mm diameter of a trephine drill. Animals were divided into three groups: no treatment (control, C), treatment with rhMMP-2 diluted in monoolein liquid crystal (rhMMP-2) and negative control with monoolein (M). The groups were divided into two experimental times, 2- and 4-weeks of experimental time. RESULTS: The rate of new-formed bone, estimated by the number of points on new-formed cancellous bone, was in enhanced rhMMP-2 group in both periods in comparison to C or M groups. CONCLUSION: There was no difference in bone neoformation between second to fourth week within groups. In the present study, monoolein alone had a negative role in the post-operative surgery, but monoolein associated with +rhMMP-2 had a positive role on releasing rhMMP-2 and enhance the rate of new-formed bone (Tab. 1, Fig. 5, Ref. 71).
Assuntos
Regeneração Óssea , Glicerídeos , Metaloproteinase 2 da Matriz , Proteínas Recombinantes , Adulto , Animais , Glicerídeos/farmacologia , Glicerídeos/uso terapêutico , Humanos , Masculino , Metaloproteinase 2 da Matriz/farmacologia , Ratos , Ratos Wistar , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/uso terapêuticoAssuntos
Asma/diagnóstico , Aplicativos Móveis/normas , Rinite Alérgica/diagnóstico , Inquéritos e Questionários/normas , Adolescente , Telefone Celular , Feminino , Humanos , Masculino , Avaliação de Resultados em Cuidados de Saúde , Medidas de Resultados Relatados pelo Paciente , Telemedicina , Adulto JovemRESUMO
Dose measurements are acknowledged to be a vital part of the quality assurance process in diagnostic radiology, and the use of thermoluminescence dosemeters (TLDs) is a recommended method of entrance dose evaluation. Measurement of doses in radiographic examinations is widely adopted in clinical practice for adults as well as for children. Phantoms can be used to simulate different parts of the body, depending on the materials used to build them. In this work two different sets of phantoms have been prepared with acrylic blocks. The first set was used to simulate children of different ages. The second set was used to simulate the adult spine. The dosimetric measurements have been carried out using TLD and an ionising chamber. Measurements were performed in three X-ray equipments in Aracaju, Brazil. The entrance, half thickness and exit surface doses were analysed.
Assuntos
Absorciometria de Fóton/métodos , Osso e Ossos/diagnóstico por imagem , Proteção Radiológica/métodos , Medição de Risco/métodos , Dosimetria Termoluminescente/métodos , Filhos Adultos , Carga Corporal (Radioterapia) , Humanos , Doses de Radiação , Fatores de RiscoRESUMO
Relapses after "curative resection" of rectal cancer may be localized and thus amenable to surgical treatment. The risk of relapse may be higher than previously believed depending on adequacy and length of follow-up, the diagnostic modalities used, and the number of autopsies performed. Technical refinements such as a distal margin greater than 2 cm, radical pelvic lymphadenectomy, and high ligation of the inferior mesenteric artery at the aorta do not appear to diminish the risk of recurrence. A wider lateral margin and a more complete excision of the mesorectum may be beneficial, but this is yet unproven. Surgery for isolated, locally recurrent lesions should be limited to carefully selected patients and performed in tertiary centers using a team approach. Whether such radical "salvage" procedures are justifiable merits further careful evaluation.