RESUMO
A novel series of 3,5-diaminoindazoles were prepared and found to be CDK inhibitors. Potent inhibitors against CDK1 and CDK2 were obtained by introduction of 1lambda(6)-isothiazolidine-1,1-dioxide at 5-position of indazole. Anti-proliferative activities of compounds were evaluated using EJ, HCT116, SW620, and A549 cancer cell lines.
Assuntos
Antineoplásicos/farmacologia , Quinases Ciclina-Dependentes/antagonistas & inibidores , Imidazóis/farmacologia , Antineoplásicos/síntese química , Linhagem Celular Tumoral , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/farmacologia , Humanos , Imidazóis/síntese química , Concentração Inibidora 50 , Modelos Químicos , Relação Estrutura-AtividadeRESUMO
The preparation of a synthetic receptor for multivalent protein binding by a directed immobilization of bifunctional ligands was demonstrated using pentameric C-reactive protein (CRP) and a thiolated phosphocholine-containing ligand on a gold surface. CRP consisting of five identical, noncovalently linked subunits and having five phosphocholine-binding sites on the same face was complexed with 12-mercaptododecylphosphocholine. The complexes were reacted with a gold surface, which was blocked with BSA or 2-mercaptoethanol to avoid non-specific binding. CRP binding to the molecularly imprinted monolayer was investigated by surface plasmon resonance, exhibiting high sensitivity with a detection limit as low as 1 pM (0.12 ng/mL) and binding affinity (K(A) ~ 10(-7)-10(-9) M(-1)) comparable to that of immobilized anti- CRP.
Assuntos
Proteína C-Reativa/química , Ouro/química , Fosforilcolina/química , Sítios de Ligação , Proteína C-Reativa/metabolismo , Humanos , Ligantes , Fosforilcolina/análogos & derivados , Propriedades de SuperfícieRESUMO
The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of human serum CRP using the MIP-based sandwich immunoassay has been demonstrated.