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1.
Biochim Biophys Acta ; 488(1): 172-8, 1977 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-889857

RESUMO

1-O-[1'-14C]Hexadecyloxyethyl rac-glycerol was administered to 18-day-old rats by intracerebral injection, and incorporation of radioactivity into the brain lipids was determined after 6, 24 and 48 h. Some of the substrate was catabolized by oxidative cleavage of either of the two ether bonds. Cleavage in the hexadecyloxyethyl moiety yielded labeled palmitic acid, whereas oxidative cleaveage of the glycol glycerol ether bond produced O-hexadecyl glycolic acid. The substrate was also incorporated as such into both ethanolamine and choline phospholipids. Evidence is presented for the desaturation by rat brain of 1-O-hexadecyloxyethyl-2-acyl-sn-glycero-3-phosphoethanolamine to the plasmalogen analogue, while the corresponding choline phospholipid was not desaturated.


Assuntos
Encéfalo/metabolismo , Fosfatidiletanolaminas/metabolismo , Plasmalogênios/biossíntese , Animais , Cinética , Masculino , Ratos
2.
Biochim Biophys Acta ; 409(3): 311-9, 1975 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-1203248

RESUMO

1-O-[1'-14C]Hexadecyl ethanediol was administered intracerebrally to myelinating rat brain, and incorporation of radioactivity into brain lipids was followed over a 48-h period: (1) O-Hexadecyl ethanediol was metabolized primarily through oxidative ether bond cleavage, and much of the label was recovered in phospholipid acyl groups. (2) Substantial amounts of radioactivity were also found in choline and ethanolamine phospholipids having an O-hexadecyloxyethyl glycerol backbone. This means that alkyl ethanediol was used in glycerol ether biosynthesis as are long-chain primary alcohols. (3) Acidic hydrolysis of the ethanolamine glycerophosphatide fraction yielded also labeled hexadecanol which may indicate desaturation of 1-O-hexadecyloxyethyl 2-acyl glycerophosphoryl ethanolamine to the plasmalogen analogue. (4) Small amounts of the substrate were oxidized to O-hexadecyl glycolic acid and incorporated into the phospholipids. The substrate did not serve as precursor of O-hexadecyl ethanediol phosphorylcholine or phosphorylethanolamine in the brain.


Assuntos
Encéfalo/metabolismo , Etilenoglicóis/metabolismo , Lipídeos/biossíntese , Animais , Masculino , Oxirredução , Fosfatidilcolinas/biossíntese , Fosfatidiletanolaminas/biossíntese , Ratos , Fatores de Tempo
3.
Chem Phys Lipids ; 17(2-3 SPEC NO): 267-74, 1976 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-991381

RESUMO

Long-chain 1,2-alkanediols can be separated into their enantiomers by thin-layer chromatography of the bis-L-acetylmandelates. The method is also applicable to the separation of enantiomeric 1,3-alkanediols. Both 2-hydroxy fatty acids and 1,2-alkanediols derived from diester waxes of rat skin have the D-configuration. The D-enantiomer of 2DL-1,2-octadecanediol is preferentially utilized by myelinating rat brain in the formation of choline phospholipids having a 1,2-octadecanediol backbone. The D-configuration of the chiral center at carbon-2 of 2-acyl-1,2-alkanediol phosphocholine corresponds to the configuration at carbon-2 of 1,2-diacyl-sn-glycero-3-phosphocholine.


Assuntos
Ácidos Graxos , Álcoois Graxos , Hidroxiácidos , Animais , Encéfalo/metabolismo , Cromatografia em Camada Fina , Ácidos Graxos/análise , Álcoois Graxos/análise , Hidroxiácidos/análise , Conformação Molecular , Bainha de Mielina/metabolismo , Fosfatidilcolinas/biossíntese , Ratos , Pele/análise , Estereoisomerismo , Relação Estrutura-Atividade
4.
J Chromatogr Sci ; 13(10): 478-86, 1975 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-241757

RESUMO

Chromatographic methods, especially thin-layer chromatography (TLC) and gas-liquid chromatography (GLC) are widely used in investigations of the occurrence, molecular structure and metabolism of ether lipids. The application of such techniques to structural analysis and quantification, in combination with methods for the degradation and derivatization of ether lipids, is discussed.


Assuntos
Cromatografia , Éteres/análise , Lipídeos/análise , Alcenos , Alquilação , Cromatografia Gasosa , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Glicerídeos/análise , Humanos , Concentração de Íons de Hidrogênio , Hidrólise , Fosfolipídeos/análise , Plasmalogênios/análise
8.
J Lipid Res ; 12(2): 198-202, 1971 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-5554108

RESUMO

A fraction comprising about 0.7% of the extractable surface lipids of cabbage (Brassica oleracea) leaves was isolated and identified as a mixture of isomeric beta-hydroxyketones consisting mainly of 14-keto-16-hydroxynonacosane and 15-keto-13-hydroxynonacosane.


Assuntos
Álcoois/análise , Cetonas/análise , Plantas/análise , Ceras/análise , Acetona/análise , Álcoois/isolamento & purificação , Aldeídos/análise , Fenômenos Químicos , Química , Cromatografia Gasosa , Cromatografia em Camada Fina , Ciclização , Éteres Cíclicos/análise , Ácidos Graxos/análise , Raios Infravermelhos , Cetonas/isolamento & purificação , Luz , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Rotação , Espectrofotometria , Estereoisomerismo
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