RESUMO
Modelling is key to adapting agriculture to climate change (CC), facilitating evaluation of the impacts and efficacy of adaptation measures, and the design of optimal strategies. Although there are many challenges to modelling agricultural CC adaptation, it is unclear whether these are novel or, whether adaptation merely adds new motivations to old challenges. Here, qualitative analysis of modellers' views revealed three categories of challenge: Content, Use, and Capacity. Triangulation of findings with reviews of agricultural modelling and Climate Change Risk Assessment was then used to highlight challenges specific to modelling adaptation. These were refined through literature review, focussing attention on how the progressive nature of CC affects the role and impact of modelling. Specific challenges identified were: Scope of adaptations modelled, Information on future adaptation, Collaboration to tackle novel challenges, Optimisation under progressive change with thresholds, and Responsibility given the sensitivity of future outcomes to initial choices under progressive change.
RESUMO
This guideline is aimed at those who are involved in the assessment of anthelmintic efficacy in ruminant livestock species (bovine, ovine and caprine). The intent is to provide a framework that can be adopted worldwide for the testing of anthelmintics in ruminants, such that studies carried out in different countries can be compared and thereby unnecessary duplication can be reduced. Recommendations are made for the selection, housing and feeding of study animals, the type of studies required, the method used to conduct those studies, the assessment of results and the standards for defining anthelmintic efficacy.
Assuntos
Anti-Helmínticos , Cabras , Ruminantes , Animais , Anti-Helmínticos/uso terapêutico , Bovinos , Ovinos , Ruminantes/parasitologia , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/parasitologia , Doenças das Cabras/tratamento farmacológico , Doenças das Cabras/parasitologia , Helmintíase Animal/tratamento farmacológico , Helmintíase Animal/parasitologia , Doenças dos Bovinos/tratamento farmacológico , Doenças dos Bovinos/parasitologiaRESUMO
Haemonchus contortus is arguably one of the most economically important and ubiquitous parasites of livestock globally and commonly involved in cases of anthelmintic resistance. Here, we performed reciprocal genetic crosses using susceptible (MHco3(ISE)) and multiple anthelmintic resistant (MHco18(UGA2004)) H. contortus isolates. Resultant admixed populations were designated MHco3/18 or MHco18/3, where the lead isolate reflects the origin of the females. Three independent filial generations were generated for each cross, which were subjected to bioassays, molecular approaches and population genetic analyses to investigate the phenotypic and genotypic inheritance of benzimidazole (BZ) resistance at each stage. A panel of microsatellite markers confirmed the success of the genetic cross as markers from both parents were seen in the F1 crosses. Egg hatch tests revealed a stark difference between the two F1 crosses with ED50 estimates for MHco18/3 being 9 times greater than those for MHco3/18. Resistance factors based on ED50 estimates ranged from 6 to 57 fold in the filial progeny compared to MHco3(ISE) parents. Molecular analysis of the F167Y and F200Y SNP markers associated with BZ resistance were analysed by pyrosequencing and MiSeq deep amplicon sequencing, which showed that MHco3/18.F1 and MHco18/3.F1 both had similar frequencies of the F200Y resistant allele (45.3% and 44.3%, respectively), whereas for F167Y, MHco18/3.F1 had a two-fold greater frequency of the resistant-allele compared to MHco3/18.F1 (18.2% and 8.8%, respectively). Comparison between pyrosequencing and MiSeq amplicon sequencing revealed that the allele frequencies derived from both methods were concordant at codon 200 (rc = 0.97), but were less comparable for codon 167 (rc = 0.55). The use of controlled reciprocal genetic crosses have revealed a potential difference in BZ resistance phenotype dependent on whether the resistant allele is paternally or maternally inherited. These findings provide new insight and prompt further investigation into the inheritance of BZ resistance in H. contortus.
Assuntos
Anti-Helmínticos , Hemoncose , Haemonchus , Animais , Anti-Helmínticos/farmacologia , Benzimidazóis/farmacologia , Cruzamentos Genéticos , Resistência a Medicamentos/genética , Feminino , Hemoncose/tratamento farmacológico , Hemoncose/epidemiologia , Hemoncose/veterinária , Fenótipo , Polimorfismo de Nucleotídeo Único , Tubulina (Proteína)/genéticaRESUMO
Anthelmintic resistance (AR) is a growing concern for effective parasite control in farmed ruminants globally. Combatting AR will require intensified and integrated research efforts in the development of innovative diagnostic tests to detect helminth infections and AR, sustainable anthelmintic treatment strategies and the development of complementary control approaches such as vaccination and plant-based control. It will also require a better understanding of socio-economic drivers of anthelmintic treatment decisions, in order to support a behavioural shift and develop targeted communication strategies that promote the uptake of evidence-based sustainable solutions. Here, we review the state-of-the-art in these different fields of research activity related to AR in helminths of livestock ruminants in Europe and beyond. We conclude that in the advent of new challenges and solutions emerging from continuing spread of AR and intensified research efforts, respectively, there is a strong need for transnational multi-actor initiatives. These should involve all key stakeholders to develop indicators of infection and sustainable control, set targets and promote good practices to achieve them.
Assuntos
Anti-Helmínticos , Helmintíase Animal , Helmintos , Nematoides , Infecções por Nematoides , Doenças dos Ovinos , Animais , Anti-Helmínticos/farmacologia , Anti-Helmínticos/uso terapêutico , Resistência a Medicamentos , Helmintíase Animal/tratamento farmacológico , Gado , Infecções por Nematoides/parasitologia , Ruminantes/parasitologia , Ovinos , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/parasitologia , Doenças dos Ovinos/prevenção & controleRESUMO
The Consortium for Anthelmintic Resistance and Susceptibility (CARS) brings together researchers worldwide, with a focus of advancing knowledge of resistance and providing information on detection methods and treatment strategies. Advances in this field suggest mechanisms and features of resistance that are shared among different classes of anthelmintic. Benzimidazole resistance is characterized by specific amino acid substitutions in beta-tubulin. If present, these substitutions increase in frequency upon drug treatment and lead to treatment failure. In the laboratory, sequence substitutions in ion-channels can contribute to macrocyclic lactone resistance, but there is little evidence that they are significant in the field. Changes in gene expression are associated with resistance to several different classes of anthelmintic. Increased P-glycoprotein expression may prevent drug access to its site of action. Decreased expression of ion-channel subunits and the loss of specific receptors may remove the drug target. Tools for the identification and genetic analysis of parasitic nematodes and a new online database will help to coordinate research efforts in this area. Resistance may result from a loss of sensitivity as well as the appearance of resistance. A focus on the presence of anthelmintic susceptibility may be as important as the detection of resistance.
Assuntos
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/genética , Anti-Helmínticos/farmacologia , Resistência a Medicamentos , Helmintos/efeitos dos fármacos , Helmintos/genética , Tubulina (Proteína)/genética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Substituição de Aminoácidos , Animais , Benzimidazóis/farmacologia , Biomarcadores , Antagonistas Colinérgicos/farmacologia , Resistência a Medicamentos/genética , Feminino , Expressão Gênica , Helmintos/metabolismo , Canais Iônicos de Abertura Ativada por Ligante/genética , Canais Iônicos de Abertura Ativada por Ligante/metabolismo , Compostos Macrocíclicos/farmacologia , Masculino , Testes de Sensibilidade Parasitária , Tubulina (Proteína)/metabolismoRESUMO
Improved animal health can reduce greenhouse gas (GHG) emissions intensity in livestock systems while increasing productivity. Integrated modelling of disease impacts on farm-scale emissions is important in identifying effective health strategies to reduce emissions. However, it requires that modellers understand the pathways linking animal health to emissions and how these might be incorporated into models. A key barrier to meeting this need has been the lack of a framework to facilitate effective exchange of knowledge and data between animal health experts and emissions modellers. Here, these two communities engaged in workshops, online exchanges and a survey to i) identify a comprehensive list of disease-related model parameters and ii) test its application to evaluating models. Fifty-six parameters were identified and proved effective in assessing the potential of farm-scale models to characterise livestock disease impacts on GHG emissions. Easy wins for the emissions models surveyed include characterising disease impacts related to feeding.
Assuntos
Gases de Efeito Estufa , Animais , Fazendas , Efeito Estufa , Gases de Efeito Estufa/análise , GadoRESUMO
We report a European wide assessment of the economic burden of gastrointestinal nematodes, Fasciola hepatica (common liver fluke) and Dictyocaulus viviparus (bovine lungworm) infections to the ruminant livestock industry. The economic impact of these parasitic helminth infections was estimated by a deterministic spreadsheet model as a function of the proportion of the ruminant population exposed to grazing, the infection frequency and intensity, the effect of the infection on animal productivity and mortality and anthelmintic treatment costs. In addition, we estimated the costs of anthelmintic resistant nematode infections and collected information on public research budgets addressing helminth infections in ruminant livestock. The epidemiologic and economic input data were collected from international databases and via expert opinion of the Working Group members of the European Co-operation in Science and Technology (COST) action COMbatting Anthelmintic Resistance in ruminants (COMBAR). In order to reflect the effects of uncertainty in the input data, low and high cost estimates were obtained by varying uncertain input data arbitrarily in both directions by 20 %. The combined annual cost [low estimate-high estimate] of the three helminth infections in 18 participating countries was estimated at 1.8 billion [ 1.0-2.7 billion]. Eighty-one percent of this cost was due to lost production and 19 % was attributed to treatment costs. The cost of gastrointestinal nematode infections with resistance against macrocyclic lactones was estimated to be 38 million [ 11-87 million] annually. The annual estimated costs of helminth infections per sector were 941 million [ 488 - 1442 million] in dairy cattle, 423 million [ 205-663 million] in beef cattle, 151million [ 90-213 million] in dairy sheep, 206 million [ 132-248 million] in meat sheep and 86 million [ 67-107 million] in dairy goats. Important data gaps were present in all phases of the calculations which lead to large uncertainties around the estimates. Accessibility of more granular animal population datasets at EU level, deeper knowledge of the effects of infection on production, levels of infection and livestock grazing exposure across Europe would make the largest contribution to improved burden assessments. The known current public investment in research on helminth control was 0.15 % of the estimated annual costs for the considered parasitic diseases. Our data suggest that the costs of enzootic helminth infections which usually occur at high prevalence annually in ruminants, are similar or higher than reported costs of epizootic diseases. Our data can support decision making in research and policy to mitigate the negative impacts of helminth infections and anthelmintic resistance in Europe, and provide a baseline against which to measure future changes.
Assuntos
Doenças dos Bovinos/economia , Efeitos Psicossociais da Doença , Infecções por Dictyocaulus/economia , Fasciolíase/veterinária , Doenças das Cabras/economia , Doenças dos Ovinos/economia , Animais , Bovinos , Dictyocaulus/fisiologia , Europa (Continente) , Fasciola hepatica/fisiologia , Fasciolíase/economia , Cabras , Ovinos , Carneiro DomésticoRESUMO
P-glycoprotein (P-gp) homologues, belonging to the ATP Binding Cassette (ABC) transporter family, are thought to play an important role in the resistance of gastro-intestinal nematode parasites against macrocyclic lactones. The aim of this study was to investigate the influence of various P-gp interfering compounds on the efficacy of ivermectin (IVM) in sensitive and resistant nematode isolates. The feeding of IVM resistant and sensitive Teladorsagia circumcincta and Haemonchus contortus first-stage larvae (L1) was assessed using a range of IVM concentrations (0.08-40 nm) with or without P-gp inhibitors: valspodar, verapamil, quercetin, ketoconazole and pluronic P85. The P-gp inhibitors were selected on the basis of their ability to interfere with P-gp transport activity in an epithelial cell line over-expressing murine P-gp. In the presence of P-gp interfering agents, the in vitro susceptibility to IVM of both sensitive and resistant isolates of T. circumcincta and H. contortus was increased. These results show that compounds interfering with P-gp transport activity could enhance IVM efficacy in sensitive isolates, and also restore IVM sensitivity in resistant nematodes. These results support the view that ABC transporters can play an important role in resistance to IVM, at least in the free-living stages of these economically important gastro-intestinal nematodes.
Assuntos
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/antagonistas & inibidores , Anti-Helmínticos/farmacologia , Resistência a Medicamentos , Ivermectina/farmacologia , Trichostrongyloidea/efeitos dos fármacos , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Animais , Transporte Biológico , Larva/efeitos dos fármacosRESUMO
Gastrointestinal (GI) nematode control has an important role to play in increasing livestock production from a limited natural resource base and to improve animal health and welfare. In this synthetic review, we identify key research priorities for GI nematode control in farmed ruminants and pigs, to support the development of roadmaps and strategic research agendas by governments, industry and policymakers. These priorities were derived from the DISCONTOOLS gap analysis for nematodes and follow-up discussions within the recently formed Livestock Helminth Research Alliance (LiHRA). In the face of ongoing spread of anthelmintic resistance (AR), we are increasingly faced with a failure of existing control methods against GI nematodes. Effective vaccines against GI nematodes are generally not available, and anthelmintic treatment will therefore remain a cornerstone for their effective control. At the same time, consumers and producers are increasingly concerned with environmental issues associated with chemical parasite control. To address current challenges in GI nematode control, it is crucial to deepen our insights into diverse aspects of epidemiology, AR, host immune mechanisms and the socio-psychological aspects of nematode control. This will enhance the development, and subsequent uptake, of the new diagnostics, vaccines, pharma-/nutraceuticals, control methods and decision support tools required to respond to the spread of AR and the shifting epidemiology of GI nematodes in response to climatic, land-use and farm husbandry changes. More emphasis needs to be placed on the upfront evaluation of the economic value of these innovations as well as the socio-psychological aspects to prioritize research and facilitate uptake of innovations in practice. Finally, targeted regulatory guidance is needed to create an innovation-supportive environment for industries and to accelerate the access to market of new control tools.
Assuntos
Animais Domésticos/parasitologia , Gastroenteropatias/veterinária , Nematoides/fisiologia , Infecções por Nematoides/veterinária , Ruminantes/parasitologia , Doenças dos Suínos/prevenção & controle , Animais , Anti-Helmínticos/uso terapêutico , Pesquisa Biomédica , Controle de Doenças Transmissíveis , Gastroenteropatias/parasitologia , Gastroenteropatias/prevenção & controle , Gado , Infecções por Nematoides/parasitologia , Infecções por Nematoides/prevenção & controle , Vacinas Protozoárias/administração & dosagem , Suínos , Doenças dos Suínos/parasitologiaRESUMO
Multiple resistance to benzimidazole, imidazothiazole and macrocyclic lactone anthelmintics is an emerging problem in the south-east of Scotland. The general management and nematode control strategies employed in four affected flocks (flocks 1-4) were investigated in an attempt to identify the risk factors which might have led to the appearance of production limiting disease associated with anthelmintic resistance. The important risk factors for multiple anthelmintic resistance could not be confirmed and it proved easier to criticise nematode control practices on theoretical grounds, than to propose practical solutions. It seems likely that different risk factors were involved in the four flocks. Lambs in flocks 1 and 2 had been treated with an anthelmintic at 3-4 weekly intervals with the aim of achieving suppressive nematode control, while sheep in flock 1 had been treated with an anthelmintic after they were moved onto clean grazing. Recently lambed ewes had been treated with moxidectin in three of the four flocks, with the aim of controlling their periparturient rise in faecal nematode egg output. All of these factors might have contributed to the emergence of multiple anthelmintic resistance, because they could have led to anthelmintic treatments at times when the nematode population in refugia was small, compared to that in the sheep. Annual rotation of the anthelmintic group was compromised by the emergence of benzimidazole resistance and did not prevent the emergence of multiple resistance in any of the flocks described, although the practice may have slowed the development of resistance. Underdosing may have selected for benzimidazole and imidazothiazole resistance in flock 2, associated with inaccurate estimation of the weights of terminal sire lambs. These investigations also highlighted problems associated with the diagnosis of anthelmintic resistance, in particular the confounding effects of the onset of host immunity to nematode parasites, the possible influence of the age of the adult nematode population, and the insensitivity of the undifferentiated faecal egg count reduction test in situations where resistance is emerging.
Assuntos
Anti-Helmínticos/farmacologia , Resistência a Múltiplos Medicamentos , Helmintíase Animal/parasitologia , Doenças dos Ovinos/parasitologia , Animais , Feminino , Helmintíase Animal/tratamento farmacológico , Helmintíase Animal/epidemiologia , Masculino , Escócia/epidemiologia , Ovinos , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/epidemiologiaRESUMO
The cause of ill thrift and deaths was investigated in a group of 40 Scottish lowground ewes, which had been treated with levamisole and then turned onto clean grazing after lambing, 6-8 weeks previously. Concurrent haemonchosis and teladorsagiosis were diagnosed, putatively associated with the spring maturation of large numbers of hypobiotic larvae. Ill thrift due to parasitic gastroenteritis consequently occurred in the lambs which had been turned onto the 'clean' grazing with their dams, despite metaphylactic anthelmintic treatments. The contributory role of haemonchosis resulting in serious production-limiting disease is unusual in Scottish sheep flocks. It is suggested that the problem may have arisen as a consequence of the adaptation of Haemonchus contortus to survive overwinter in a cold climate as arrested early fourth stage larvae, which were not eliminated when the ewes were treated with levamisole at turnout.
Assuntos
Hemoncose/veterinária , Doenças dos Ovinos/parasitologia , Animais , Anti-Helmínticos/uso terapêutico , Feminino , Hemoncose/diagnóstico , Hemoncose/epidemiologia , Hemoncose/parasitologia , Ivermectina/uso terapêutico , Larva , Escócia/epidemiologia , Estações do Ano , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/epidemiologia , Fatores de TempoRESUMO
Thirty-eight sheep flocks, predominantly from the south/central Scotland, were examined using a faecal egg count reduction test (FECRT) for the presence of ivermectin (IVM) resistant nematodes. Efficacies of less than 95%, 14-17 days post-treatment, were identified in 6 of 17 naturally grazing flocks where pre-treatment faecal egg counts were in excess of 150 eggs per gram. Efficacies on these IVM resistant farms ranged from 66 to 92%. One other suspected cases of IVM resistance was also identified in returned material. The larvae detected in post-treatment coprocultures from resistant flocks were from the genera Teladorsagia (4 from 6) and Trichostrongylus (2 from 6).
Assuntos
Antiparasitários/farmacologia , Ivermectina/farmacologia , Contagem de Ovos de Parasitas/veterinária , Doenças dos Ovinos/parasitologia , Animais , Resistência a Medicamentos , Fezes/parasitologia , Feminino , Masculino , Testes de Sensibilidade Parasitária/veterinária , Ovinos , Doenças dos Ovinos/tratamento farmacológico , Resultado do TratamentoRESUMO
The in vitro ovicidal activity of the amino acetonitrile derivative, monepantel (MPTL) and its active metabolite monepantel sulfone (MPTL-SO2) were assessed against a number of commercially important nematode species of ruminants, namely Teladorsagia circumcincta, Haemonchus contortus and Trichostrongylus axei. An egg hatch test (EHT) was used to make the assessment of both drug sensitive and drug resistant isolates. Both MPTL and MPTL-SO2 showed moderate ovicidal activity in vitro against all of the species examined, although species specific differences as measured by inhibitory concentration were observed. Analysis of the drug sensitive isolates showed H. contortus to be the most sensitive to both MPTL and MPTL-SO2 (ED50 1.7 and 2.7 µg/ml respectively) followed by T. circumcincta (ED50 2.1 and 2.7 µg/ml respectively) followed by T. axei (ED50 68.7 and 60.1 µg/ml respectively). Overall the EHT results would suggest no "global" in vitro discriminatory dose for detection of MPTL resistance is likely to be achievable, using the egg hatch test, due to large inherent variability observed between species. The test identified a dose dependent increase in MPTL and MPTL-SO2 sensitivity in two MPTL resistant T. circumcincta isolates and therefore offers to be a promising tool for the phenotypic characterisation of MPTL sensitivity, allowing exploration into the mechanisms involved in selection and development of MPTL resistance.
Assuntos
Aminoacetonitrila/análogos & derivados , Haemonchus/efeitos dos fármacos , Sulfonas/farmacologia , Trichostrongylus/efeitos dos fármacos , Aminoacetonitrila/farmacologia , Animais , Anti-Helmínticos/farmacologia , Resistência a Medicamentos , Embrião não Mamífero/efeitos dos fármacos , Concentração Inibidora 50 , Fenótipo , Zigoto/efeitos dos fármacosRESUMO
The impact of climate change on parasites and parasitic diseases is a growing concern and numerous empirical and mechanistic models have been developed to predict climate-driven spatial and temporal changes in the distribution of parasites and disease risk. Variation in parasite phenotype and life-history traits between isolates could undermine the application of such models at broad spatial scales. Seasonal variation in the transmission of the haematophagous gastrointestinal nematode Haemonchus contortus, one of the most pathogenic helminth species infecting sheep and goats worldwide, is primarily determined by the impact of environmental conditions on the free-living stages. To evaluate variability in the development success and mortality of the free-living stages of H. contortus and the impact of this variability on future climate impact modelling, three isolates of diverse origin were cultured at a range of temperatures between 15°C and 37°C to determine their development success compared with simulations using the GLOWORM-FL H. contortus model. No significant difference was observed in the developmental success of the three isolates of H. contortus tested, nor between isolates and model simulations. However, development success of all isolates at 37°C was lower than predicted by the model, suggesting the potential for overestimation of transmission risk at higher temperatures, such as those predicted under some scenarios of climate change. Recommendations are made for future climate impact modelling of gastrointestinal nematodes.
Assuntos
Mudança Climática , Hemoncose/veterinária , Haemonchus/fisiologia , Modelos Biológicos , Animais , Doenças das Cabras/epidemiologia , Doenças das Cabras/parasitologia , Cabras , Hemoncose/epidemiologia , Hemoncose/parasitologia , Haemonchus/classificação , Medição de Risco , Estações do Ano , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/parasitologiaRESUMO
Anthelmintic efficacies against juvenile developing populations of Teladorsagia species that were known to be resistant to anthelmintics from all three broad spectrum families were examined using a controlled efficacy test. Fenbendazole (FBZ), levamisole (LEV), ivermectin (IVM), combinations of these anthelmintics and moxidectin (MOX) were assessed in parasite naïve lambs artificially infected with 8,000 third stage larvae (Tci5) and treated orally 8-day post-infection with the compounds at the manufacturers recommended dose rates, FBZ, 5 mg/kg body weight (BW); LEV, 7.5 mg/kg BW; IVM, 0.2 mg/kg BW; MOX (0.2 mg/kg BW). The lambs were slaughtered 14-day post-treatment. The arithmetic mean worm burden reductions resulting from oral treatments with FBZ; IVM; LEV; FBZ+IVM; FBZ+LEV; FBZ, LEV+IVM or MOX were 36%, 82%, 38%, 86%, 60%, 88% and 97%, respectively. The results illustrate that combination treatments showed improved efficacies against the juvenile population compared to individually administered treatments but that these improvements were not wholly effective. Moxidectin was the only treatment that was over 95% effective, though caution should be noted when advising the use of MOX prophylactically since 3% of the infection still survived this treatment. Treatments directed at juvenile stages of Tci5 were less effective, with the exception of IVM, compared to a similar trial using Tci5 where the same treatments were directed against a predominantly adult population. No interaction was detected comparing the timings of treatments and its effectiveness with the exception of IVM (two-way ANOVA, p < 0.05). These findings suggest that, on the whole, the selection processes for anthelmintic resistance (AR) may occur at an early stage of development within the parasites, having severe implications for the early detection of AR.
Assuntos
Antinematódeos/uso terapêutico , Gastroenteropatias/veterinária , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/parasitologia , Trichostrongyloidea/crescimento & desenvolvimento , Tricostrongiloidíase/tratamento farmacológico , Tricostrongiloidíase/veterinária , Abomaso/parasitologia , Animais , Resistência a Medicamentos , Quimioterapia Combinada , Fenbendazol/uso terapêutico , Gastroenteropatias/tratamento farmacológico , Gastroenteropatias/parasitologia , Ivermectina/uso terapêutico , Levamisol/uso terapêutico , Macrolídeos/uso terapêutico , Tricostrongiloidíase/parasitologiaRESUMO
Control of a benzimidazole-, levamisole- and ivermectin-resistant population of Teladorsagia circumcincta was attempted using moxidectin administered orally at the manufacturer's recommended dose rate of 200 microg/kg bodyweight. Ewes were dosed after lambing with the aim of controlling their periparturient rise in faecal egg output and lambs were dosed at six-week intervals throughout the summer. This regimen failed to suppress the establishment of significant numbers of infective helminth larvae on the pasture, resulting in unsatisfactory lamb production. Oral dosing with moxidectin was effective in removing adult female burdens of ivermectin-resistant T. circumcincta, but the effect of the drug did not persist against the resistant helminth population.
Assuntos
Anti-Helmínticos/uso terapêutico , Macrolídeos/uso terapêutico , Doenças dos Ovinos/tratamento farmacológico , Trichostrongyloidea/efeitos dos fármacos , Tricostrongiloidíase/veterinária , Administração Oral , Animais , Animais Recém-Nascidos , Anti-Helmínticos/farmacologia , Benzimidazóis/farmacologia , Benzimidazóis/uso terapêutico , Fezes/parasitologia , Feminino , Gastroenteropatias/tratamento farmacológico , Gastroenteropatias/parasitologia , Gastroenteropatias/veterinária , Ivermectina/farmacologia , Ivermectina/uso terapêutico , Levamisol/farmacologia , Levamisol/uso terapêutico , Macrolídeos/farmacologia , Contagem de Ovos de Parasitas/veterinária , Ovinos , Trichostrongyloidea/crescimento & desenvolvimento , Tricostrongiloidíase/tratamento farmacológico , Tricostrongiloidíase/parasitologiaRESUMO
Monepantel (MPTL) is one of two new anthelmintic compounds introduced onto the sheep market to control gastro-intestinal nematodes. Resistance to this compound is rare but has been reported. In order to preserve the efficacy of this and other anthelmintics, it is essential to understand both (a) the mechanisms involved in the selection of resistance and (b) how the parasites evolve to deal with these compounds. To address these questions three MPTL-resistant Teladorsagia circumcincta isolates (MTci2-11, MTci5-13 and MTci7-12) have been artificially selected in vivo from phenotypically characterised parent isolates (MTci2, MTci5, MTci7 respectively). The selection process involved collecting and culturing eggs from surviving worms from sheep administered sub-optimal dosages of MPTL (Zolvix®) to provide infective larvae to infect further sheep until resistant isolates were generated (between 9 and 13 rounds of selection). A controlled efficacy test was conducted using the original parental isolates and the newly generated MPTL resistant isolates (n = 5 per group). Selected isolates were assessed both under anthelmintic stress (Zolvix®, 2.5 mg/kg bodyweight; MTci-MPTL) and at rest (untreated, MTci-CON). A number of life-history traits were assessed, namely, worm establishment rates, time to patency, faecal egg output, body length of adults and eggs in utero. The estimated resistance status of the selected isolates was confirmed with 48%, 28% and 9% reductions in worm burden at 7-days post Zolvix® administration for MTci2-11-MPTL, MTci5-13-MPTL and MTci7-12-MPTL, respectively, compared with untreated controls. One of the selected isolates MTci7-12-CON showed significantly greater total worm burden (p = 0.025), greater establishment rate (p = 0.033), decreased time to patency (p = 0.048), higher cumulative egg outputs (p = 0.002) compared with its parental derivative MTci7. The trial results suggest that anthelmintic selection in T. circumcincta, albeit under experimental conditions, can select for more prolific/fecund and quicker maturing populations. These data provide an insight into how parasites evolve in response to anthelmintic pressure.
Assuntos
Aminoacetonitrila/análogos & derivados , Anti-Helmínticos/farmacologia , Nematoides/efeitos dos fármacos , Doenças dos Ovinos/parasitologia , Aminoacetonitrila/farmacologia , Animais , Resistência a Medicamentos , Fezes/parasitologia , Feminino , Masculino , Contagem de Ovos de Parasitas/veterinária , Seleção Genética , Ovinos , Doenças dos Ovinos/tratamento farmacológicoRESUMO
The anthelmintic resistance status of two field isolates derived from farms (farm A and B) located near Edinburgh were examined using both controlled efficacy tests (CET) and faecal egg count reduction tests (FECRT). Efficacies against fenbendazole (FBZ), levamisole (LEV) and ivermectin (IVM) and, for one isolate, against combinations of these anthelmintics and moxidectin were determined in naïve lambs, artificially infected with the isolates and treated with the compounds at the manufacturers recommended dose rates. (FBZ, 5mg/kg bodyweight (BW); LEV, 7.5mg/kg BW; IVM, 0.2mg/kg BW; Moxidectin (MOX) 0.2mg/kg BW). In both field isolates, the predominant species found pre-treatment and the only species found post-treatment was Teladorsagia circumcincta. Resistance to FBZ, LEV and IVM was confirmed in CET and FECRT on farm A and to the latter two compounds on farm B, which had a history of benzimidazole resistance and where TBZ resistance was also demonstrated using an egg hatch assay (EHA). For the farm A isolate CET efficacies against FBZ; IVM; LEV; FBZ + IVM; FBZ + LEV; FBZ, LEV + IVM and MOX were 59, 60, 88, 94,93, 92 and 98%, respectively. The CET efficacies for the farm B isolate were 51% and 72% for LEV and IVM, respectively.
Assuntos
Antinematódeos/farmacologia , Gastroenteropatias/veterinária , Doenças dos Ovinos/parasitologia , Trichostrongyloidea/efeitos dos fármacos , Tricostrongiloidíase/veterinária , Abomaso/parasitologia , Animais , Antinematódeos/uso terapêutico , Resistência a Medicamentos , Quimioterapia Combinada , Fezes/parasitologia , Fenbendazol/farmacologia , Fenbendazol/uso terapêutico , Gastroenteropatias/tratamento farmacológico , Gastroenteropatias/parasitologia , Ivermectina/farmacologia , Ivermectina/uso terapêutico , Levamisol/farmacologia , Levamisol/uso terapêutico , Macrolídeos/farmacologia , Macrolídeos/uso terapêutico , Contagem de Ovos de Parasitas/veterinária , Escócia , Ovinos , Doenças dos Ovinos/tratamento farmacológico , Trichostrongyloidea/isolamento & purificação , Tricostrongiloidíase/tratamento farmacológico , Tricostrongiloidíase/parasitologiaRESUMO
An in vitro direct challenge (IVDC) method in which abomasal tissue explants maintained at 37 degrees C in Hanks/Hepes solution (pH 7.6) in a high oxygen concentration (incubator gassed with pure oxygen and sealed prior to use) were-challenged with exsheathed third stage larvae of Teladorsagia circumcincta has been used to investigate the tissue association phase of larval establishment. Studies using naïve and recently challenged immune sheep and goat abomasal tissues have shown significant reductions (P < 0.05) in the numbers of tissue associated larvae in material from immune animals. The mechanisms that affect the tissue association process appear to be labile since more larvae were recovered from the tissue digests of previously infected sheep that had not been recently exposed to larval challenge in comparison to those that had been recently challenged (P < 0.05). The method has also been used to demonstrate the influence of protein nutrition on the establishment of larvae in abomasal tissues and region specific differences in the efficacy of exclusion mechanisms. The technique appears to have potential as a means of investigating the crucial first phase of the process of establishment.