RESUMO
BACKGROUND: Around birth, myocardial substrate supply changes from carbohydrates before birth to primarily fatty acids after birth. Parallel to these changes, the myocardium is expected to switch from the use of primarily lactate before birth to fatty acids thereafter. However, myocardial lactate uptake and oxidation around birth has not been measured in vivo. METHODS AND RESULTS: We measured myocardial lactate uptake, oxidation, and release with infusion of [1-13C]lactate and myocardial flux of fatty acids and glucose in chronically instrumented fetal and newborn (1 to 15 days) lambs. Myocardial lactate oxidation was the same in newborn (81.7+/-14.7 micromol. min-1. 100 g-1, n=11) as in fetal lambs (60.7+/-26.7 micromol. min-1. 100 g-1, n=7). Lactate uptake was also the same in newborn as in fetal lambs. Lactate uptake was higher than lactate flux, indicating lactate release simultaneously with uptake. In the newborn lambs, lactate uptake declined with age. Lactate uptake was strongly related to lactate supply, whereas lactate oxidation was not. The supply of fatty acids or glucose did not interfere with lactate uptake, but the flux of fatty acids was inversely related to lactate oxidation. CONCLUSIONS: We show that lactate is an important energy source for the myocardium before birth as well as in the first 2 weeks after birth in lambs. We also show that there is release of lactate by the myocardium simultaneously with uptake of lactate. Furthermore, we show that lactate oxidation may be attenuated by fatty acids but not by glucose, probably at the level of pyruvate dehydrogenase.
Assuntos
Animais Recém-Nascidos/metabolismo , Coração/embriologia , Ácido Láctico/metabolismo , Miocárdio/metabolismo , Envelhecimento/metabolismo , Animais , Animais Recém-Nascidos/sangue , Animais Recém-Nascidos/crescimento & desenvolvimento , Artérias , Metabolismo Energético/fisiologia , Ácidos Graxos/metabolismo , Sangue Fetal/metabolismo , Feto/metabolismo , Glucose/metabolismo , Ácido Láctico/sangue , Oxirredução , Ovinos/embriologiaRESUMO
BACKGROUND: Lactate accounts for a third of myocardial oxygen consumption before and in the first 2 weeks after birth. It is unknown how the remainder of myocardial oxygen is consumed. Glucose is thought to be important before birth, whereas long-chain fatty acids (LC-FA) are the prime substrate for the adult. However, the ability of the myocardium of the newborn to use LC-FA has been doubted. METHODS AND RESULTS: We measured the myocardial metabolism of glucose and LC-FA with [U-(13)C]glucose and [1-(13)C]palmitate in chronically instrumented fetal and newborn lambs. In fetal lambs, myocardial oxidation of glucose was high and that of LC-FA was low. Glucose and LC-FA accounted for 48+/-4% and 2+/-2% of myocardial oxygen consumption, respectively. In newborn lambs, oxidation of glucose decreased, whereas oxidation of LC-FA increased. Glucose and LC-FA accounted for 12+/-3% and 83+/-19% of myocardial oxygen consumption. To test whether near-term fetal lambs could use LC-FA, we increased the supply of LC-FA with a fat infusion. In fetal lambs during fat infusion, the oxidation of LC-FA increased 15-fold. Although the oxidation of LC-FA was still lower than in newborn lambs, the contribution to myocardial oxygen consumption (70+/-13%) was the same as in newborn lambs. CONCLUSIONS: These data show that glucose and lactate account for the majority of myocardial oxygen consumption in fetal lambs, whereas in newborn lambs, LC-FA and lactate account for the majority of myocardial oxygen consumption. Moreover, we showed that the fetal myocardium can use LC-FA as an energy substrate.
Assuntos
Coração/embriologia , Miocárdio/metabolismo , Animais , Animais Recém-Nascidos , Radioisótopos de Carbono , Metabolismo Energético , Feminino , Glucose/metabolismo , Coração/crescimento & desenvolvimento , Consumo de Oxigênio/fisiologia , Ácido Palmítico/metabolismo , Gravidez , OvinosRESUMO
This study describes the efficacy of oral sotalol in the treatment and prevention of atrial flutter in children after surgery for congenital heart disease. In 11 of 13 children (85%), conversion to sinus rhythm was achieved, and in 8 of 11 within 24 hours.
Assuntos
Antiarrítmicos/uso terapêutico , Flutter Atrial/tratamento farmacológico , Cardiopatias Congênitas/cirurgia , Complicações Pós-Operatórias/tratamento farmacológico , Sotalol/uso terapêutico , Adolescente , Flutter Atrial/complicações , Criança , Pré-Escolar , Cardiopatias Congênitas/complicações , Humanos , Lactente , Resultado do TratamentoRESUMO
OBJECTIVE: Because of either cardiac anatomy or small size, pacing in children often occurs by means of epicardial leads. The disadvantage of epicardial leads is the shorter longevity of these leads compared with endocardial leads. During short-term follow-up, improved stimulation thresholds were found for the newer steroid-eluting epicardial leads. The longevity of these leads may be better than that of conventional epicardial leads. An improved longevity of epicardial leads may influence the choice to either epicardial or endocardial pacing in children. METHODS: We studied the longevity and the pacing and sensing characteristics of 33 steroid-eluting epicardial pacing leads (group I, 15 atrial, 18 ventricular) implanted between November 1991 and October 1996 in 20 children with a mean age of 7.6 +/- 6.5 years (mean +/- SD), and 29 endocardial pacing leads (group II, 15 atrial, 14 ventricular) implanted during the same period in 21 children with a mean age of 11.7 +/- 4.7 years. RESULTS: The mean follow-up in group I was 2.9 +/- 1.6 years and in group II 3.1 +/- 1.7 years (P =.61). The 2-year survival of the leads in group I was 91% +/- 5% and in group II 86% +/- 7% (P =.97). Lead failure occurred in both groups in 4 leads (P =.85). Chronic stimulation and sensing thresholds were similar. CONCLUSIONS: Steroid-eluting epicardial leads have the same longevity as the conventional endocardial leads. Pacing and sensing thresholds were similar and did not change during follow-up. Therefore steroid-eluting epicardial pacing leads are a good alternative for endocardial leads in small children and in children with congenital heart disease.
Assuntos
Estimulação Cardíaca Artificial/métodos , Marca-Passo Artificial , Criança , Falha de Equipamento , Feminino , Seguimentos , Humanos , MasculinoRESUMO
In a previous study we found, after an overnight fast of 18 hours, a lower arterial glucose concentration and a depressed glycogenolysis in lambs with aortopulmonary left-to-right shunts. During exercise, glucose and free fatty acids (FFA) concentrations normally increase. The aim of this study was to investigate whether the shunt lambs could compensate for a depressed glycogenolysis by increasing gluconeogenesis and by increasing levels of blood substrates such as FFA and glycerol during exercise. Therefore, we investigated glucose kinetics, with [U-(13)C]glucose, in five 7-week-old shunt and 7 control lambs of a similar age, at rest and during moderate exercise (treadmill; 50% of VO(2) peak). The glucose production rate and the rate of disappearance of glucose were lower in shunt than in control lambs, both at rest and during exercise. We found no difference in metabolic clearance rate of glucose, glucose recycling, or gluconeogenesis between both groups of lambs. Glycogenolysis was at rest lower in shunt than in control lambs and tended to be lower during exercise. The arterial concentrations of pyruvate, lactate, FFA, and total and free glycerol increased during exercise in both groups of lambs. In conclusion, shunt lambs have lower arterial glucose concentrations than control lambs, both at rest and during moderate exercise. This was due to a lower glucose production rate, in particular a lower glycogenolysis. In addition, the reduced glycogenolysis rate was not offset by an increase in gluconeogenesis nor by an increase in other substrates that can be utilized by working muscles.
Assuntos
Aorta/fisiologia , Esforço Físico/fisiologia , Artéria Pulmonar/fisiologia , Algoritmos , Animais , Aorta/cirurgia , Gasometria , Glicemia/metabolismo , Metabolismo Energético/fisiologia , Epinefrina/sangue , Gluconeogênese/fisiologia , Glicogênio/sangue , Hemodinâmica/fisiologia , Norepinefrina/sangue , Consumo de Oxigênio/fisiologia , Artéria Pulmonar/cirurgia , OvinosRESUMO
Spontaneously occurring hypoglycemia has been described in children with severe acute congestive heart failure. Hypoglycemia may be the result of an increase in glucose utilization in tissues, a decrease in glucose production, or a decrease in the dietary intake of nutrients. To determine whether hypoglycemia may also occur in congenital heart disease with volume overloading, we investigated glucose metabolism during and after an 18-hour fast in nine lambs with an aortopulmonary left-to-right shunt and nine control lambs. Plasma levels of hormones involved in the endocrine control of glucose metabolism were determined. The glucose production rate (rate of appearance [Ra]) was studied using [U-13C]glucose. Gluconeogenesis through the Cori cycle was estimated by measuring glucose 13C recycling. The arterial glucose concentration (3,409 +/- 104 v 4,338 +/- 172 micromol/L, P < .001) and Ra of glucose (16.97 +/- 0.89 v 25.49 +/- 4.28 micromol x min(-1) x kg(-1), P < .05) were lower in shunt versus control lambs. There were no differences in hormone levels between control and shunt lambs. Fractional glucose 13C recycling via the Cori cycle (6.9% +/- 2.8% v 7.1% +/- 2.5%) and gluconeogenesis from pyruvate and lactate (1.24 +/- 0.58 v 1.95 +/- 0.67 micromol x min(-1) x kg(-1)) were similar in both groups of lambs. The sum of glycogenolysis and gluconeogenesis from precursors other than pyruvate and lactate was lower in shunt versus control lambs (15.73 +/- 1.07 v 23.54 +/- 4.27 micromol x min(-1) x kg(-1), P < .05). In conclusion, after an 18-hour fast, the arterial glucose concentration is lower in lambs with aortopulmonary shunts. This lower glucose concentration is associated with a decreased glucose production rate. In shunt lambs, glycogenolysis is decreased, while there is no difference in gluconeogenesis or hormonal control.
Assuntos
Glicemia/análise , Insuficiência Cardíaca/metabolismo , Hipoglicemia/metabolismo , Animais , Isótopos de Carbono , Modelos Animais de Doenças , Jejum , Gluconeogênese , Glicólise , Derivação Cardíaca Esquerda , Hemodinâmica , OvinosRESUMO
In a previous study [G. C. M. Beaufort-Krol, J. Takens, M. C. Molenkamp, G. B. Smid, J. J. Meuzelaar, W. G. Zijlstra, and J. R. G. Kuipers. Am. J. Physiol. 275 (Heart Circ. Physiol. 44): H1503-H1512, 1998], a lower systemic O2 supply was found in lambs with aortopulmonary left-to-right shunts. To determine whether the lower systemic O2 supply results in increased anaerobic metabolism, we used [1-13C]lactate to investigate lactate kinetics in eight 7-wk-old lambs with shunts and eight control lambs, at rest and during moderate exercise [treadmill; 50% of peak O2 consumption (VO2)]. The mean left-to-right shunt fraction in the shunt lambs was 55 +/- 3% of pulmonary blood flow. Arterial lactate concentrations and the rate of appearance (Ra) and disappearance (Rd) of lactate were similar in shunt and control lambs, both at rest (lactate: 1, 201 +/- 76 vs. 1,214 +/- 151 micromol/l; Ra = Rd: 12.97 +/- 1.71 vs. 12.55 +/- 1.25 micromol. min-1. kg-1) and during a similar relative workload. We found a positive correlation between Ra and systemic blood flow, O2 supply, and VO2 in both groups of lambs. In conclusion, shunt lambs have similar lactate kinetics as do control lambs, both at rest and during moderate exercise at a similar fraction of their peak VO2, despite a lower systemic O2 supply.
Assuntos
Aorta/fisiologia , Ácido Láctico/sangue , Esforço Físico/fisiologia , Artéria Pulmonar/fisiologia , Descanso/fisiologia , Algoritmos , Animais , Aorta/cirurgia , Gasometria , Pressão Sanguínea/fisiologia , Epinefrina/sangue , Frequência Cardíaca/fisiologia , Cinética , Norepinefrina/sangue , Oxigênio/sangue , Artéria Pulmonar/cirurgia , Circulação Pulmonar/fisiologia , OvinosRESUMO
Substrate oxidation by various organs in animals as well as in humans is usually studied by experiments in which radioactively labeled substrates are used and the production of 14CO2 is measured. In vivo, substrate oxidation by an organ has, up to now, not been determined by means of stable isotopes. Problems in the determination of the concentration of 13CO2 in blood may have impeded the use of 13C-labeled substrates. For the determination of 13CO2 concentration in blood a direct method for the determination of total CO2 concentration in blood was combined with the determination of the isotope ratio (13C/12C) of CO2 by isotope ratio mass spectrometry. The intra-assay relative standard deviation of the CO2 concentration (mean: 19.26 mmol l-1; n = 7) was 0.8%. The inter-assay relative standard deviation of the CO2 concentration in solutions of a weighed amount of Na2CO3 determined over a 5 year period was 0.64% (mean: 21.99 mmol l-1; n = 22). The intra-assay relative standard deviation of 13C in CO2 was 0.03% (mean 13C/12C: 0.0111557; n = 5). From the 13CO2 concentration in arterial and venous blood, substrate oxidation by various organs can be calculated. As an illustration, the determination of myocardial glucose oxidation in lambs, both at rest and during exercise, is described.
Assuntos
Dióxido de Carbono/análise , Dióxido de Carbono/sangue , Glucose/metabolismo , Espectrometria de Massas/métodos , Miocárdio/metabolismo , Oxirredução , Animais , Radioisótopos de Carbono , Humanos , Esforço Físico/fisiologia , Ovinos , Especificidade por SubstratoRESUMO
OBJECTIVE: Total cavopulmonary connection (TCPC) is a routine operation for palliation of children with cardiac anomalies in whom biventricular repair is impossible. The original technique consists of the creation of a semi-prosthetic intercaval tunnel. A substantial proportion of these patients develop atrial flutter. We developed a technique for creating an intercaval tunnel that uses the tissue of the right auricle as intercaval tunnel. This technique avoids suture lines in the neighbourhood of the blood supply of the sinus node and leaves the terminal crest free. Since atrial flutter frequently occurs after Mustard and Senning operations in which suture lines are similar as for creating the lateral tunnel in TCPC we postulated that our technique for creating the intercaval tunnel without prosthetic material might prevent atrial flutter. METHODS: All the children that qualified for a TCPC were included. Whenever possible our operative technique was applied. In the other cases a semi-prosthetic conduit was used for creating the intercaval tunnel. Of 47 consecutive patients 30 (64%) had a tunnel of right auricle tissue, 12 (26%) had a tunnel of prosthetic material. Five patients did not need an intercaval tunnel and were omitted in this study. Only surviving patients were included in this study. Patients that needed more atrial surgery then necessary for TCPC were also omitted. Postoperative ECG's and Holter monitorings were studied. RESULTS: Overall mortality was 7 of 47 patients (14.9% 70% CL 9.4-22.2%). There was no mortality due to rhythm disturbances. Atrial flutter occurred in 3 of 31 included patients (9.7, 70% CL 4.3-18.5%). In the right auricle group 1 of 22 patients (4.5, 70% CL 0.6-14.6%) had atrial flutter compared to 2 of 9 patients (22.2, 70% CL 7.5-45.0%) in the prosthesis group (P=0.13). CONCLUSION: The use of the right auricular technique for creating the intercaval tunnel TCPC is applicable in the majority of patients qualifying for a TCPC. Mortality and morbidity are equal comparing the two techniques. However, markedly less atrial flutter occurs in the group where the right auricle was used as intercaval tunnel. Therefore, we recommend the use of our technique for total cavopulmonary connection.
Assuntos
Flutter Atrial/prevenção & controle , Derivação Cardíaca Direita/métodos , Cardiopatias Congênitas/cirurgia , Flutter Atrial/epidemiologia , Estudos de Casos e Controles , Pré-Escolar , Técnica de Fontan , Humanos , IncidênciaRESUMO
BACKGROUND: We recently identified a novel mutation in large family characterised by premature nocturnal sudden death. In the present paper we provide an overview of the findings in this family. METHODS: From 1958 onwards, when the first patient presented, we collected clinical data on as many family members as possible. After identification in 1998 of the underlying genetic disorder (SCN5A, 1795insD), genotyping was performed diagnostically. RESULTS: Since 1905 unexplained sudden death occurred in 26 family members, 17 of whom died during the night. Besides sudden death, symptomatology was rather limited; only six patients reported syncopal attacks. In one of them, a 13-year-old boy, asystolic episodes up to nine seconds were documented. Until now, the mutation has been found in 114 family members (57 males, 57 females). Carriers of the mutant gene exhibited bradycardia-dependent QT-prolongation, intrinsic sinus node dysfunction, generalised conduction abnormalities, a paucity of ventricular ectopy, and the Brugada sign. Cardiomyopathy or other structural abnormalities were not found in any of the carriers. Electrophysiological studies showed that mutant channels were characterised by markedly reduced INa amplitude, a positive shift of voltage-dependence of activation and a substantial negative shift of voltage-dependence of inactivation of INa. From 1978 onwards, a pacemaker for anti-brady pacing was implanted for prevention of sudden death. In patients in whom a prophylactic pacemaker was implanted no unexplained sudden death occurred, whereas 5 sudden deaths occurred in the group of patients who did not receive a pacemaker. CONCLUSION: We have described a large family with a SCN5A-linked disorder (1795insD) with features of LQT3, Brugada syndrome and familial conduction system disease. Anti-brady pacing was successful in preventing sudden death. The mode of death is possibly bradycardic.
RESUMO
The case-history of a 7-year-old boy with endocarditis, myocarditis and pericarditis, possibly caused by Coxiella burnetii (Q-fever), is presented. As far as we know only one case of Q-fever endocarditis in a child is previously described in literature. That child had, contrary to our patient, a pre-existing valve abnormality. Q-fever was diagnosed after demonstration of a significant rise in titer of the antibodies against Coxiella burnetii in both the IgM and the IgG fractions. IgA antibodies, indicative for an endocarditis, were also demonstrated.
Assuntos
Endocardite Bacteriana/etiologia , Miocardite/etiologia , Pericardite/etiologia , Febre Q , Doença Aguda , Antígenos de Bactérias/isolamento & purificação , Criança , Coxiella/imunologia , Endocardite Bacteriana/complicações , Humanos , Masculino , Miocardite/complicações , Pericardite/complicações , Febre Q/diagnósticoAssuntos
Propafenona/uso terapêutico , Taquicardia Supraventricular/tratamento farmacológico , Taquicardia Ventricular/tratamento farmacológico , Administração Oral , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Propafenona/administração & dosagem , Resultado do TratamentoRESUMO
Atrial tachycardias, in particular atrial flutter after surgery for congenital heart disease, is associated with a high mortality. Treatment with various antiarrhythmic drugs and/or antitachycardia pacemakers is not very successful. Sotalol, a Class III drug, has shown to be a promising drug in adults with atrial tachycardias. However, the experience with sotalol in children after surgery for congenital heart disease is limited. Therefore, we describe our results here. Between December 1990 and February 1997, 26 children with atrial tachycardias, most of them with atrial flutter or fibrillation (n = 20), after surgery for congenital heart disease were treated with sotalol orally. The age of the children at the start of treatment was 7.5 +/- 5.8 years (mean +/- SD). The time interval between surgery and the start of atrial tachycardia ranged from 1 day to 14.3 years (3.8 +/- 3.8 years). Conversion to sinus rhythm was achieved in 16 out of 22 hemodynamically stable children with a dosage of 4.0 +/- 1.6 mg/kg per day. The six children without sinus rhythm on sotalol and four hemodynamically unstable patients were treated prophylactically with sotalol after DC cardioversion for their tachycardias. Two children complained of mild transient fatigue. Heart rate decreased during therapy (95 +/- 33 vs 81 +/- 21 beats/min; P = 0.01). QTc-intervals did not change. Proarrhythmias such as torsades de pointes were not encountered. Two children with a preexistent sick sinus syndrome showed aggravation of bradycardia and needed pacemaker implantation. The percentage of children with a recurrence-free interval of 1 and 2 years was 96% and 81%, respectively, for all atrial tachycardias, and 92% and 66% for atrial flutter. The recurrences of atrial tachycardias during the follow-up period, which ranged from 0.1-6.1 years (2.5 +/- 1.8 years) could be treated with only an increase of the dosage of sotalol in all but one patient. We conclude that sotalol is an effective drug for the treatment and prevention of atrial tachycardia in children after surgery for congenital heart disease.
Assuntos
Antiarrítmicos/uso terapêutico , Cardiopatias Congênitas/cirurgia , Complicações Pós-Operatórias/tratamento farmacológico , Sotalol/uso terapêutico , Taquicardia/tratamento farmacológico , Administração Oral , Adulto , Antiarrítmicos/administração & dosagem , Antiarrítmicos/efeitos adversos , Fibrilação Atrial/tratamento farmacológico , Fibrilação Atrial/prevenção & controle , Flutter Atrial/tratamento farmacológico , Flutter Atrial/prevenção & controle , Bradicardia/fisiopatologia , Bradicardia/terapia , Estimulação Cardíaca Artificial , Criança , Intervalo Livre de Doença , Cardioversão Elétrica , Eletrocardiografia/efeitos dos fármacos , Fadiga/induzido quimicamente , Seguimentos , Átrios do Coração , Frequência Cardíaca/efeitos dos fármacos , Humanos , Marca-Passo Artificial , Complicações Pós-Operatórias/prevenção & controle , Recidiva , Síndrome do Nó Sinusal/tratamento farmacológico , Síndrome do Nó Sinusal/fisiopatologia , Síndrome do Nó Sinusal/terapia , Sotalol/administração & dosagem , Sotalol/efeitos adversos , Taxa de Sobrevida , Taquicardia/prevenção & controle , Fatores de Tempo , Resultado do TratamentoRESUMO
At the age of 4 years, a total cavopulmonary connection was performed in a boy with a complex congenital heart defect. On addition, a DDDR pacemaker was implanted for sick sinus syndrome. Atrial and ventricular leads were epicardially placed at the left atrium and left ventricle. At the age of 10 years, a new epicardial ventricular lead was placed because of malfunction of the existing lead. At the same operation the pulse generator was replaced by a Medtronic Kappa DR 731. After replacement, the boy experienced episodes of phrenic nerve stimulation associated with feelings of discomfort. Holter recordings revealed ventricular stimulation from the atrial stimulus for 2 consecutive beats. This phenomenon repeated exactly every 3 hours and was caused by the automatic lead impedance measurement that used a 5-V, 1-ms stimulus output.
Assuntos
Marca-Passo Artificial/efeitos adversos , Síndrome do Nó Sinusal/terapia , Criança , Impedância Elétrica , Estimulação Elétrica/instrumentação , Eletrocardiografia Ambulatorial , Desenho de Equipamento , Falha de Equipamento , Átrios do Coração/anormalidades , Derivação Cardíaca Direita , Humanos , Masculino , Nervo FrênicoRESUMO
Reactive hyperaemia, which occurs after a period of arterial occlusion is the result of an autoregulatory mechanism, involving local factors and autonomic nerve system control. To determine this autoregulatory mechanism in cutaneous blood flow in premature newborns measured the cutaneous reactive hyperaemia response after a 1 min occlusion, using a diode laser Doppler flowmeter applied to the skin. Twenty-four infants with a gestational age ranging from 25 to 37 weeks and a postnatal age of 0.3 to 72 days were studied. The reactive hyperaemia response is described by the parameters maximal cutaneous blood flow (Vmax), the time to reach maximal flow (tmax), the time taken for blood flow to return to baseline following Vmax (t(end)) and the % increase in cutaneous blood flow above preocclusional level (overshoot). A cutaneous reactive hyperaemia response could be elicited in all infants. Following occlusion the Vmax was 2,640 +/- 1,050 mV (mean +/- SD), the tmax was 7.6 +/- 3.9 sec (mean +/- SD), the t(end) was 35.6 +/- 14.6 sec (mean +/- SD) and the overshoot was 74.6 +/- 34% (mean +/- SD). A negative correlation was found between hematocrit and tmax (r = -0.62, p less than 0.01). No influence of postconceptional age, postnatal age, skin and rectal temperature, incubator temperature, weight, and transcutaneous oxygen tension on the reactive hyperaemia parameters could be found. We conclude that reactive hyperaemia of cutaneous blood flow can be elicited in premature newborn infants irrespective of postconceptional age, and that the hematocrit is inversely related to the time to reach maximal flow after occlusion.
Assuntos
Velocidade do Fluxo Sanguíneo , Hiperemia/fisiopatologia , Recém-Nascido Prematuro/fisiologia , Reperfusão , Pele/irrigação sanguínea , Fatores Etários , Hematócrito , Homeostase , Humanos , Hiperemia/sangue , Hiperemia/diagnóstico , Recém-Nascido , UltrassonografiaRESUMO
Free fatty acids are the major fuels for the myocardium, but during a higher load carbohydrates are preferred. Previously, we demonstrated that myocardial net lactate uptake was higher in lambs with aortopulmonary shunts than in control lambs. To determine whether this was caused by an increased lactate uptake and oxidation or by a decreased lactate release, we studied myocardial lactate and glucose metabolism with 13C-labeled substrates in 36 lambs in a fasting, conscious state. The lambs were assigned to two groups: a resting group consisting of 8 shunt and 9 control lambs, and an exercise group (50% of peak O2 consumption) consisting of 9 shunt and 10 control lambs. Myocardial lactate oxidation was higher in shunt than in control lambs (mean +/- SE, rest: 10.33 +/- 2.61 vs. 0. 17 +/- 0.82, exercise: 38.05 +/- 8.87 vs. 16.89 +/- 4.78 micromol. min-1. 100 g-1; P < 0.05). There was no difference in myocardial lactate release between shunt and control lambs. Oxidation of exogenous glucose, which was approximately zero at rest, increased during exercise in shunt and control lambs. The contribution of glucose and lactate to myocardial oxidative metabolism increased during exercise compared with at rest in both shunt and control lambs. We conclude that myocardial lactate oxidation is higher in shunt than in control lambs, both at rest and during exercise, and that the contribution of carbohydrates in myocardial oxidative metabolism in shunt lambs is higher than in control lambs. Thus it appears that this higher contribution of carbohydrates occurs not only in the case of pressure-overloaded hearts but also in myocardial hypertrophy due to volume overloading.
Assuntos
Derivação Arteriovenosa Cirúrgica , Ácido Láctico/metabolismo , Miocárdio/metabolismo , Condicionamento Físico Animal , Animais , Vasos Coronários/metabolismo , Vasos Coronários/cirurgia , Glucose/metabolismo , Oxirredução , OvinosRESUMO
INTRODUCTION: We recently identified a novel mutation of SCN5A (1795insD) in a large family with features of both long QT syndrome type 3 and the Brugada syndrome. The purpose of this study was to detail the clinical features and efficacy of pacemaker therapy in preventing sudden death in this family. METHODS AND RESULTS: The study group consisted of 116 adult family members: 60 carriers (29 males) and 56 noncarriers (28 males) of the mutant gene. Investigations included 24-hour Holter monitoring, ergometry, and electrophysiologic studies. Mean, lowest, and highest heart rate were lower in the carriers, but heart rate variability was comparable. In carriers, disproportional QT prolongation was present during bradycardia. No complex ventricular ectopy was recorded, and there were fewer isolated premature beats (both ventricular and atrial) in carriers. All patients were asymptomatic, except for two individuals who experienced syncope; in one of these patients, asystolic episodes (up to 9 sec) were repeatedly recorded. Prolonged HV intervals were present in 5 of 6 patients. Thirty carriers received a prophylactic backup pacemaker. During median follow-up of 4.5 years (range 0.0 to 22.6), their survival rate was 100%. There were five sudden deaths among the remaining 30 carriers without a pacemaker (P = 0.019). CONCLUSION: This family with a high incidence of nocturnal sudden death is characterized by bradycardia-dependent QT prolongation, intrinsic sinus node dysfunction, and generalized conduction abnormalities. There is a striking absence of complex ventricular ectopy, and pacemaker implantation was effective in preventing sudden death. These findings raise the possibility of a bradycardic rather than tachycardic mode of death.
Assuntos
Bradicardia/fisiopatologia , Bradicardia/terapia , Bloqueio de Ramo/fisiopatologia , Bloqueio de Ramo/terapia , Síndrome do QT Longo/fisiopatologia , Síndrome do QT Longo/terapia , Marca-Passo Artificial , Adulto , Bradicardia/genética , Bloqueio de Ramo/genética , Causas de Morte , Morte Súbita/etiologia , Eletrocardiografia , Eletrocardiografia Ambulatorial , Eletrofisiologia , Teste de Esforço , Feminino , Frequência Cardíaca/fisiologia , Humanos , Síndrome do QT Longo/genética , Masculino , Pessoa de Meia-IdadeRESUMO
Cardiac conduction disorders slow the heart rhythm and cause disability in millions of people worldwide. Inherited mutations in SCN5A, the gene encoding the human cardiac sodium (Na+) channel, have been associated with rapid heart rhythms that occur suddenly and are life-threatening; however, a chief function of the Na+ channel is to initiate cardiac impulse conduction. Here we provide the first functional characterization of an SCN5A mutation that causes a sustained, isolated conduction defect with pathological slowing of the cardiac rhythm. By analysing the SCN5A coding region, we have identified a single mutation in five affected family members; this mutation results in the substitution of cysteine 514 for glycine (G514C) in the channel protein. Biophysical characterization of the mutant channel shows that there are abnormalities in voltage-dependent 'gating' behaviour that can be partially corrected by dexamethasone, consistent with the salutary effects of glucocorticoids on the clinical phenotype. Computational analysis predicts that the gating defects of G514C selectively slow myocardial conduction, but do not provoke the rapid cardiac arrhythmias associated previously with SCN5A mutations.