RESUMO
The influence of the peritoneal cells unstimulated by an inflammatory agent (PCs) on the bone marrow erythropoiesis in the CBA mice was studied in the normal conditions and after the massive haemorrhage. The PCs obtained from the intact or anemic donors (2, 4 and 18 h after the haemorrhage) were introduced intraperitoneally into the intact syngeneic recipients. The massive haemorrhage induces a special type of differentiation of erythroid cells in bone marrow, "reserve erythropoiesis", which is characterized by a decrease, within four days, in the proliferation of basophilic proerythrocytes, stimulation of proliferation of polychromatophilic proerythrocytes and entry of oxyphilic proerythrocytes into mitosis. The transplantation of the PCs from the anemic donors induced the "reserve erythropoiesis" in the bone marrow of the recipients. The transplantation of the PCs from the intact donors did not induce the "reserve erythropoiesis" and appeared to inhibit erythropoiesis via lengthening the time of mitoses in polychromatophilic and basophilic proerythrocytes.
Assuntos
Células da Medula Óssea , Eritrócitos/citologia , Hemorragia/sangue , Cavidade Peritoneal/citologia , Anemia/sangue , Animais , Diferenciação Celular , Contagem de Eritrócitos , Eritropoese , Macrófagos/transplante , Masculino , Camundongos , Camundongos Endogâmicos CBA , Índice Mitótico , Reticulócitos/citologia , Fatores de Tempo , Transplante IsogênicoRESUMO
Peritoneal exudate cells are involved in the regulation of erythroid cell proliferation and hemoglobin synthesis. However, activation of these processes occurs independently of each other and is regulated by various mechanisms. Hemoglobin synthesis is initiated after changes in pH and/or water-electrolyte balance in the abdominal cavity. Peritoneal exudate cells gaining specific activity under conditions of hemorrhage play a role in stimulation of erythroblast proliferation.
Assuntos
Apoptose/fisiologia , Eritropoese/fisiologia , Exsudatos e Transudatos/metabolismo , Hemoglobinas/biossíntese , Hemorragia/fisiopatologia , Cavidade Peritoneal/citologia , Animais , Exsudatos e Transudatos/química , Concentração de Íons de Hidrogênio , Masculino , Camundongos , Camundongos Endogâmicos CBA , Equilíbrio Hidroeletrolítico/fisiologiaRESUMO
Thirty minutes after massive blood loss, peritoneal cells gained the ability to trigger reparative erythropoiesis in the bone marrow of normal syngeneic recipients. This was manifested in proliferation of oxyphilic erythroblasts and activation of the reserve pathway of erythroid differentiation 4 days after cell transplantation. The maximum transfer capacity of peritoneal cells was observed 4 h after blood loss, but 4 days later they only initiated mitoses in oxyphilic erythroblasts.
Assuntos
Líquido Ascítico/patologia , Comunicação Celular , Eritropoese , Transferência Adotiva , Animais , Diferenciação Celular , Hemorragia , Masculino , Camundongos , Camundongos Endogâmicos CBA , Fatores de TempoRESUMO
Experiments on mice have shown that the peritoneal cells and splenocytes transferred from anemic animals to normal syngenic recipients cause the appearance of signs of blood formation by the reserve erythropoiesis type, i.e. a decrease of the mitotic index in basophilic erythroblasts and appearance of mitotic oxyphilic erythroblasts. The capacity of peritoneal cells (PC) to transfer this type of the cell differentiation becomes manifest at 30 minutes, achieves its maximum at 2-4 hours, and becomes somewhat reduced at 48 hours after the blood loss. The transfer effect depends on the dose of introduced cells. The PC transfer capacity is higher than that of splenocytes. The lymphocyte inactivation by monoclonal anti-Thy-1,2 antibodies reduces the PC transfer capacity, which is indicative of the role played by the cell cooperation in promoting the transfer effect. Stimulation of erythropoiesis under the effect of macrophages and lymphocytes taken from anemic donors is significant and is accompanied by increased numbers of erythroid cells in the bone marrow and of reticulocytes in the peripheral blood.
Assuntos
Eritropoese , Linfócitos/fisiologia , Macrófagos/fisiologia , Anemia/sangue , Anemia/fisiopatologia , Animais , Diferenciação Celular , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Índice Mitótico , Peritônio/fisiologia , Reticulócitos/fisiologia , Baço/citologia , Baço/fisiologia , Fatores de TempoRESUMO
Transplantation of allogenic bone marrow from HLA-identical sibs to patients with acute and chronic leukemia receiving immunosuppressive therapy is associated with the appearance of erythrocytes simultaneously carrying donor and recipient antigenic markers: AB0 system, rhesus factor and its subtypes, M and N antigens. Integration of genes responsible for each antigen is realized independently presumably at the level of stem cell, which ensures long-term (>3 years) repopulation of these erythrocytes. Experiments on inbred mice showed that transplantation of allogenic bone marrow is associated with an increase of chromosome number in 39% bone marrow cells 4 days after transplantation, which indicate the possibility of integration of whole chromosomes.