RESUMO
Congenital coronary artery fistulas are infrequent but sometimes hemodynamically important anomalies depending on their magnitude and the cardiac chamber or vascular site involved. Fistula from left circumflex artery to coronary sinus are potentially curable causes of ischemic heart disease.
Les fistules congénitales des artères coronaires sont des anomalies peu fréquentes, mais parfois importantes sur le plan hémodynamique, en fonction de leur taille et de la chambre cardiaque ou du site vasculaire impliqué. Les fistules entre l'artère circonflexe et le sinus coronaire sont des causes potentiellement curables des cardiopathies ischémiques.
Assuntos
Doença da Artéria Coronariana , Anomalias dos Vasos Coronários , Fístula Vascular , Angiografia Coronária , Doença da Artéria Coronariana/diagnóstico por imagem , Doença da Artéria Coronariana/cirurgia , Seio Coronário , Anomalias dos Vasos Coronários/diagnóstico por imagem , Anomalias dos Vasos Coronários/cirurgia , Humanos , Fístula Vascular/diagnóstico por imagem , Fístula Vascular/cirurgiaRESUMO
Permanent residence at high-altitude and chronic mountain sickness (CMS) may alter the cerebrovascular homeostasis and orthostatic responses. Healthy male participants living at sea-level (LL; n = 15), 3800 m (HL3800m; n = 13) and 5100 m (HL5100m; n = 17), respectively, and CMS highlanders living at 5100 m (n = 31) were recruited. Middle cerebral artery mean blood flow velocity (MCAv), cerebral oxygen delivery (CDO2), mean blood pressure (MAP), heart rate variability and spontaneuous cardiac baroreflex sensitivity (cBRS) were assessed while sitting, initial 30 s and after 3 min of standing. Cerebral autoregulation index (ARI) was estimated (ΔMCAv%baseline)/ΔMAP%baseline) in response to the orthostatic challenge. Altitude and CMS were associated with hypoxemia and elevated hemoglobin concentration. While sitting, MCAv and LFpower negatively correlated with altitude but were not affected by CMS. CDO2 remained preserved. BRS was comparable across all altitudes, but lower with CMS. Within initial 30 s of standing, altitude and CMS correlated with a lesser ΔMAP while ARI remained unaffected. After 3 min standing, MCAv, CDO2 and cBRS remained preserved across altitudes. The LF/HF ratio increased in HL5100m compared to LL and HL3800m from sitting to standing. In contrary, CMS showed blunted autonomic nervous activation in responses to standing. Despite altitude- and CMS-associated hypoxemia, erythrocytosis and impaired blood pressure regulation (CMS only), cerebral homeostasis remained overall preserved.
Assuntos
Doença da Altitude , Altitude , Barorreflexo , Pressão Sanguínea , Circulação Cerebrovascular , Frequência Cardíaca , Homeostase , Humanos , Masculino , Doença da Altitude/fisiopatologia , Adulto , Pressão Sanguínea/fisiologia , Frequência Cardíaca/fisiologia , Barorreflexo/fisiologia , Circulação Cerebrovascular/fisiologia , Pessoa de Meia-Idade , Velocidade do Fluxo Sanguíneo , Artéria Cerebral Média/fisiopatologia , Hipóxia/fisiopatologiaRESUMO
Arginine is the sole substrate for nitric oxide (NO) synthesis by NO synthases (NOS) and promotes the proliferation and maturation of human T-cells. Arginine is also metabolized by the enzyme arginase, producing urea and ornithine, the precursor for polyamine production. We sought to determine the molecular mechanisms regulating arginase and NOS in splenic immune cells after trauma. C3H/HeN mice underwent laparotomy as simulated moderate trauma or anesthesia alone (n = 24 per group). Six, 12, 24, or 48 h later, 6 animals from each group were sacrificed, and splenectomy was performed and plasma collected. Six separate animals had neither surgery nor anesthesia and were sacrificed to provide resting values (t = 0 h). Spleen arginase I and II and iNOS mRNA abundance, arginase I protein expression, and arginase activity were determined. Plasma NO metabolites (nitrite + nitrate) were also measured. Trauma increased spleen arginase I protein expression and activity (P = 0.01) within 12 and for at least 48 h after injury and coincided with up-regulated arginase I mRNA abundance at 24 h. Neither arginase II nor iNOS mRNA abundance in the spleen was significantly increased by trauma at 24 h. Plasma nitrite + nitrate was decreased in animals 48 h post-injury compared to anesthesia controls (P < 0.05). Trauma induces up-regulation of arginase I gene expression in splenic immune cells within 24 h of injury. Arginase II is not significantly up-regulated at that time point. Arginase I, rather than iNOS appears to be the dominant route for arginine metabolism in splenic immune cells 24 h after trauma.
Assuntos
Arginina/metabolismo , Enzimas/metabolismo , Baço/metabolismo , Ferimentos e Lesões/metabolismo , Animais , Arginase/genética , Arginase/metabolismo , Enzimas/genética , Regulação Enzimológica da Expressão Gênica , Isoenzimas , Camundongos , Camundongos Endogâmicos C3H , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase/genética , Óxido Nítrico Sintase/metabolismo , Óxido Nítrico Sintase Tipo II , RNA Mensageiro/metabolismoRESUMO
BACKGROUND: Arginase, which metabolizes L-arginine within the urea cycle, is essential for production of polyamines and affects production of nitric oxide by depletion of L-arginine, the common substrate for both arginase and nitric oxide synthase. Having shown that trauma increases splenic macrophage arginase activity, we seek to define the mechanisms for this. RAW macrophage arginase activity and expression are increased by 8-bromo-cAMP in vitro. We hypothesize that since catecholamines increase cAMP, trauma-induced splenic arginase activity may be mediated by post-injury catecholamine release. METHODS: RAW 264.7 macrophage arginase activity was measured in vitro in response to 4 catecholamines with or without propranolol or lipopolysaccharide (LPS). C57BL/6 mice underwent laparotomy as a model of moderate trauma after propranolol treatment, with and without intraperitoneal Escherichia coli LPS administration as a simulated pro-inflammatory stimulus. RESULTS: Macrophage arginase activity increased in vitro in response to catecholamines or LPS (P < .05). Propranolol pretreatment blocked macrophage arginase activity induced by epinephrine (10 mumol/L) in vitro (P < .05). Trauma or LPS alone increased splenic arginase activity in vivo (P < .05). Propranolol did not alter LPS-induced splenic arginase activity but did significantly reduce trauma-induced splenic arginase activity (P < .05). CONCLUSIONS: Catecholamines alone increase macrophage arginase activity through beta-adrenoceptor activation. Increased splenic arginase activity induced by moderate trauma is decreased by beta-adrenoceptor blockade, suggesting that trauma-induced arginase activity is partly mediated by endogenous catecholamines.
Assuntos
8-Bromo Monofosfato de Adenosina Cíclica/farmacologia , Arginase/metabolismo , Isoproterenol/farmacologia , Macrófagos/fisiologia , Propranolol/farmacologia , Receptores Adrenérgicos beta/fisiologia , Agonistas Adrenérgicos beta/farmacologia , Antagonistas Adrenérgicos beta/farmacologia , Animais , Arginase/biossíntese , Linhagem Celular , Dopamina/farmacologia , Indução Enzimática , Epinefrina/farmacologia , Cinética , Laparotomia , Lipopolissacarídeos/farmacologia , Macrófagos/enzimologia , Camundongos , Camundongos Endogâmicos C57BL , Norepinefrina/farmacologia , Receptores Adrenérgicos beta/efeitos dos fármacos , Baço/efeitos dos fármacos , Baço/enzimologia , Ferimentos e Lesões/enzimologiaRESUMO
BACKGROUND: Although expressed primarily in the liver, arginase activity also is present in extrahepatic tissues and specifically in macrophages, where it may play diverse physiologic roles in wound healing, cellular proliferation, and the regulation of nitric oxide production. Arginase activity in immune cells is upregulated by certain cytokines such as IL-4, IL-10, and TGF-beta and by catecholamines. Since the release of these substances is increased after trauma, we hypothesized that arginase activity would also be increased in immune cells after trauma. The current work tests this hypothesis. METHODS: A model of surgical trauma was created in C3H/HeN mice by performing an exploratory laparotomy. Tissue arginase activity and arginase I protein expression were determined. As a control, arginase activity and expression were also stimulated with the use of endotoxin. In addition, we evaluated the expression of inducible nitric oxide synthase and the accumulation of nitric oxide metabolites in plasma. RESULTS: Surgical trauma was associated with a significant increase in arginase activity in splenic and renal tissues (P < .05). Splenic macrophages from trauma animals exhibited arginase activity levels approximately 10 times those of controls (P < .05). Endotoxin alone increased arginase activity in the spleen, but this increase was less than that of trauma alone (P < .05). Arginase activity remained elevated after trauma for up to 4 days and normalized by day 7. Arginase I expression was upregulated by trauma in both splenic and renal tissue and by endotoxin in the spleen only. Despite upregulation of inducible nitric oxide synthase in trauma animals, circulating nitric oxide metabolites were decreased 2 days after trauma compared with controls (P < .05). Endotoxin-induced nitric oxide metabolites were also reduced in trauma animals compared with endotoxin treatment alone (P < .05), but this normalized by day 4. CONCLUSIONS: Extrahepatic arginase expression and activity is increased after trauma and may provide the necessary precursors for cellular proliferation and repair or may play a regulatory role in the production of nitric oxide.
Assuntos
Arginase/biossíntese , Laparotomia/efeitos adversos , Macrófagos/enzimologia , Animais , Arginase/genética , Linhagem Celular , Regulação Enzimológica da Expressão Gênica , Rim/enzimologia , Lipopolissacarídeos/toxicidade , Fígado/enzimologia , Pulmão/enzimologia , Masculino , Camundongos , Camundongos Endogâmicos C3H , Músculo Esquelético/enzimologia , Óxido Nítrico/sangue , Óxido Nítrico Sintase/biossíntese , Óxido Nítrico Sintase/genética , Óxido Nítrico Sintase Tipo II , Baço/enzimologia , Ferimentos e LesõesRESUMO
In patients with portal hypertension, vascular lesions in the colon may develop that have been collectively termed portal hypertensive colopathy. Endoscopic diagnosis of these lesions is now established, but the management of hemorrhage from them is not. We report five cases of endoscopic management of bleeding colonic vascular lesions in patients with portal hypertension. Endoscopy data from January 1, 1996 to June 30, 1999 identified 158 patients with portal hypertension who underwent colonoscopy. Forty-five of these 158 patients had portal hypertensive colopathy (angiodysplasias or varices). Those who had colonoscopic hemostasis attempted were identified and reviewed. Five patients underwent colonoscopic intervention for bleeding. Initial hemostasis was achieved in four of five cases. Repeat endoscopic intervention was necessary in three of the four cases. One patient required surgery. In patients with portal hypertension, colonic vascular lesions may develop. Hemorrhage from these lesions is rare. Colonoscopy is effective for localization and diagnosis of bleeding vascular lesions and permits simultaneous hemostatic intervention.
Assuntos
Colonoscopia , Varizes Esofágicas e Gástricas/complicações , Varizes Esofágicas e Gástricas/cirurgia , Hemostasia Cirúrgica/métodos , Hipertensão Portal/complicações , Adulto , Idoso , Feminino , Humanos , Masculino , Estudos RetrospectivosRESUMO
This investigation evaluated the risks of a simulation drill designed to improve the skill of anesthesia personnel in dealing with an unexpected difficult intubation. In a controlled prospective study, 40 patients with normal airways scheduled to undergo noncardiothoracic surgery were randomized into two groups of 20 patients. In the control group, intubation was performed by standard techniques. In the simulation group, intubation of a difficult airway was simulated and performed with the aid of an endotracheal tube introducer. Heart rate (HR); systolic, mean, and diastolic blood pressures (BPs); and arterial oxygen saturation were measured noninvasively during the preinduction period and 1 minute postintubation. A record was kept of all adverse events, including electrocardiogram (EKG) evidence of myocardial ischemia or cardiac arrhythmias, esophageal intubation, pulmonary aspiration, or tracheal injury. There were no significant differences in percent changes in HR, BP, or oxygen saturation between the two groups. There were five uncomplicated esophageal intubations in the simulation group compared with none in the control group (p = 0.001). No other adverse events were recorded. The potential hazards of esophageal intubation should be considered before this simulation drill is performed.
Assuntos
Intubação Intratraqueal/métodos , Ensino/métodos , Adulto , Idoso , Pressão Sanguínea , Esôfago , Frequência Cardíaca , Humanos , Intubação Intratraqueal/efeitos adversos , Intubação Intratraqueal/instrumentação , Laringoscópios , Masculino , Pessoa de Meia-Idade , Consumo de Oxigênio , Distribuição Aleatória , Segurança , Traqueia/lesõesRESUMO
Sciatic hernia is a rare pelvic floor hernia that occurs through the greater or lesser sciatic foramen. Sciatic hernias often present as pelvic pain, particularly in women, and diagnosis can be difficult. Transabdominal and transgluteal operative approaches, including laparoscopic repair, have been reported. We show a laparoscopic technique using a plug of human allogeneic dermal matrix and lightweight polypropylene extraperitoneal patch avoiding fixation. The patient had significant improvement of her symptoms and no complications at 12 months follow-up.
Assuntos
Hérnia Abdominal/cirurgia , Laparoscopia/métodos , Diafragma da Pelve/cirurgia , Nervo Isquiático , Idoso , Diagnóstico Diferencial , Feminino , Hérnia Abdominal/diagnóstico , Humanos , Imageamento por Ressonância Magnética , Polipropilenos , Telas Cirúrgicas , Tomografia Computadorizada por Raios XRESUMO
BACKGROUND: Arginase is a metabolic enzyme for the amino acid arginine that participates in the immune response to trauma. We hypothesize that surgical trauma induces arginase expression and activity in the human immune system. METHODS: Peripheral mononuclear cell (MNC) arginase activity and expression and plasma nitric oxide metabolites and interleukin (IL)-10 were measured in patients undergoing elective general surgery. Twenty-two healthy volunteers served as a comparison population. RESULTS: MNC arginase activity increased within 6 hours of surgery (p < 0.05) and coincided with increased arginase I protein expression. Plasma nitric oxide metabolites decreased significantly postoperatively (p < 0.05). Patients lacking an elevation in IL-10 failed to demonstrate increased MNC arginase activity. CONCLUSION: Increased MNC arginase expression may contribute to postsurgical immune dysfunction by affecting arginine use and availability and nitric oxide metabolism in the immune system. Plasma IL-10 may play a role in regulating MNC arginase activity.
Assuntos
Arginase/metabolismo , Sistema Imunitário/enzimologia , Leucócitos Mononucleares/enzimologia , Procedimentos Cirúrgicos Operatórios , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise de Variância , Estudos de Casos e Controles , Feminino , Humanos , Sistema Imunitário/metabolismo , Interleucina-10/metabolismo , Interleucina-6/metabolismo , Leucócitos Mononucleares/metabolismo , Fígado/metabolismo , Masculino , Pessoa de Meia-Idade , Óxido Nítrico/sangue , Óxido Nítrico/metabolismoRESUMO
OBJECTIVE: To determine the effect of trauma on arginase, an arginine-metabolizing enzyme, in cells of the immune system in humans. SUMMARY BACKGROUND DATA: Arginase, classically considered an enzyme exclusive to the liver, is now known to exist in cells of the immune system. Arginase expression is induced in these cells by cytokines interleukin (IL) 4, IL-10, and transforming growth factor beta, corresponding to a T-helper 2 cytokine profile. In contrast, nitric oxide synthase expression is induced by IL-1, tumor necrosis factor, and gamma interferon, a T-helper 1 cytokine profile. Trauma is associated with a decrease in the production of nitric oxide metabolites and a state of immunosuppression characterized by an increase in the production of IL-4, IL-10, and transforming growth factor beta. This study tests the hypothesis that trauma increases arginase activity and expression in cells of the immune system. METHODS: Seventeen severely traumatized patients were prospectively followed up in the intensive care unit for 7 days. Twenty volunteers served as controls. Peripheral mononuclear cells were isolated and assayed for arginase activity and expression, and plasma was collected for evaluation of levels of arginine, citrulline, ornithine, nitrogen oxides, and IL-10. RESULTS: Markedly increased mononuclear cell arginase activity was observed early after trauma and persisted throughout the intensive care unit stay. Increased arginase activity corresponded with increased arginase I expression. Increased arginase activity coincided with decreased plasma arginine concentration. Plasma arginine and citrulline levels were decreased throughout the study period. Ornithine levels decreased early after injury but recovered by postinjury day 3. Increased arginase activity correlated with the severity of trauma, early alterations in lactate level, and increased levels of circulating IL-10. Increased arginase activity was associated with an increase in length of stay. Plasma nitric oxide metabolites were decreased during this same period. CONCLUSIONS: Markedly altered arginase expression and activity in cells of the human immune system after trauma have not been reported previously. Increased mononuclear cell arginase may partially explain the benefit of arginine supplementation for trauma patients. Arginase, rather than nitric oxide synthase, appears to be the dominant route for arginine metabolism in immune cells after trauma.